FastQCFastQC Report
Mon 6 Jun 2016
ERR1211546_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211546_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4888435
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188170.38492891896895426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175000.3579877813656109No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115490.2362514792566537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94450.048.3393061
GTATCAA151300.035.0552671
TACCTGG48200.034.0456432
GTACCTG49950.033.7941931
ACCTGGG56150.028.1196863
TCAACGC189400.027.6640574
ATCAACG189300.027.629023
CAACGCA192400.027.1347315
TATCAAC196200.026.9370772
AACGCAG199150.026.3094086
GTACATG154350.024.5838571
ACGCAGA225700.023.1937077
TACATGG159200.022.7716462
CGCAGAG230950.022.6054218
ACATGGG156550.022.3626983
CATGGGG90100.021.80084
GAGTACT167250.020.13146812-13
ATGGGGG59400.019.935665
GCAGAGT256850.019.8500799
ATAACGC5400.019.1447583
Produced by FastQC (version 0.11.5)