##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211546_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4888435 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13993496896246 33.0 31.0 34.0 30.0 34.0 2 32.35681030841159 34.0 31.0 34.0 31.0 34.0 3 32.369944573263226 34.0 31.0 34.0 30.0 34.0 4 36.00420318568212 37.0 35.0 37.0 35.0 37.0 5 35.9331366787121 37.0 35.0 37.0 35.0 37.0 6 35.95124574633804 37.0 35.0 37.0 35.0 37.0 7 35.87875403886929 37.0 35.0 37.0 35.0 37.0 8 35.874272850104376 37.0 35.0 37.0 35.0 37.0 9 37.52723294878627 39.0 37.0 39.0 35.0 39.0 10-11 37.491621244836026 39.0 37.0 39.0 35.0 39.0 12-13 37.410008622391416 39.0 37.0 39.0 34.0 39.0 14-15 38.650029712985855 40.0 38.0 41.0 34.0 41.0 16-17 38.58143649245618 40.0 38.0 41.0 34.0 41.0 18-19 38.573880290931555 40.0 38.0 41.0 34.0 41.0 20-21 38.51215419658848 40.0 38.0 41.0 34.0 41.0 22-23 38.401404846336305 40.0 38.0 41.0 34.0 41.0 24-25 38.294464179231184 40.0 38.0 41.0 34.0 41.0 26-27 38.12286334992692 40.0 38.0 41.0 33.0 41.0 28-29 38.15090708989687 40.0 38.0 41.0 33.5 41.0 30-31 38.06410548570248 40.0 37.5 41.0 33.0 41.0 32-33 37.867228059696 40.0 37.0 41.0 33.0 41.0 34-35 37.73894508160588 40.0 37.0 41.0 33.0 41.0 36-37 37.671738501176755 40.0 37.0 41.0 33.0 41.0 38-39 37.56566488047811 40.0 37.0 41.0 32.5 41.0 40-41 37.399001418654436 40.0 36.5 41.0 31.5 41.0 42-43 37.24674000983955 40.0 36.0 41.0 31.0 41.0 44-45 37.06015002756506 40.0 36.0 41.0 31.0 41.0 46-47 36.799697551465854 39.0 36.0 41.0 30.0 41.0 48-49 36.573224661880545 39.0 35.0 41.0 30.0 41.0 50-51 36.36862993984782 39.0 35.0 41.0 29.0 41.0 52-53 36.17141385740017 39.0 35.0 40.0 28.5 41.0 54-55 35.97863897136814 39.0 35.0 40.0 28.0 41.0 56-57 35.78110223415061 38.0 34.5 40.0 28.0 41.0 58-59 35.525413961728034 38.0 34.0 40.0 27.0 41.0 60-61 35.21868992837176 38.0 34.0 40.0 26.0 41.0 62-63 34.8996356707208 37.5 34.0 40.0 26.0 41.0 64-65 34.59052232462946 37.0 33.0 40.0 26.0 41.0 66-67 34.25288164821666 36.5 33.0 39.0 25.5 41.0 68-69 33.86161123140637 36.0 32.5 39.0 25.0 40.0 70-71 33.483438155565125 35.5 32.0 39.0 24.0 40.0 72-73 33.075799514568565 35.0 31.5 38.0 23.5 40.0 74-75 32.66757725120617 35.0 31.0 37.0 22.5 39.5 76-77 31.124044505040978 33.5 29.5 35.5 21.0 38.5 78-79 31.94842991673204 34.0 31.0 36.0 22.0 39.0 80-81 31.909778998800228 34.5 31.0 36.0 22.0 38.0 82-83 31.619639209685715 34.0 31.0 35.5 21.5 37.0 84-85 31.317423981294628 34.0 30.5 35.0 20.0 37.0 86-87 31.012550847050232 34.0 30.0 35.0 20.0 36.0 88-89 30.73060826215343 34.0 30.0 35.0 19.5 36.0 90-91 30.44691481015908 34.0 29.5 35.0 19.0 36.0 92-93 30.233559002011894 34.0 29.0 35.0 18.5 35.0 94-95 30.014276348156415 34.0 29.0 35.0 18.0 35.0 96-97 29.801388992591697 34.0 29.0 35.0 18.0 35.0 98-99 29.63435015500871 34.0 29.0 35.0 17.0 35.0 100 27.774256587230884 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31520894228982144 1101 2 0.26480803528664865 1101 3 0.30168076598771876 1101 4 0.17551235551273692 1101 5 0.1923656459893266 1101 6 0.17154312738954758 1101 7 0.22285537796739163 1101 8 0.19557472669323772 1101 9 0.19716501392762353 1101 10-11 0.15820630796813617 1101 12-13 0.2517421791128953 1101 14-15 0.35088218116253245 1101 16-17 0.34913149008795585 1101 18-19 0.34188717340241936 1101 20-21 0.32044133384057005 1101 22-23 0.334516295405173 1101 24-25 0.2860913111129264 1101 26-27 0.2242861776053715 1101 28-29 0.30386230529023806 1101 30-31 0.4067112118897569 1101 32-33 0.4380949501096296 1101 34-35 0.3707733851636803 1101 36-37 0.37998794352179033 1101 38-39 0.3707127406152395 1101 40-41 0.3956504729340722 1101 42-43 0.38983017883435167 1101 44-45 0.3831444738318126 1101 46-47 0.4155755300390851 1101 48-49 0.37103053355048843 1101 50-51 0.4122174345321028 1101 52-53 0.46483577733854275 1101 54-55 0.35701975672838415 1101 56-57 0.34560681344611055 1101 58-59 0.4410378748230599 1101 60-61 0.41406623260615305 1101 62-63 0.4678236099450359 1101 64-65 0.4271325415969258 1101 66-67 0.4354165742984648 1101 68-69 0.3716697756382814 1101 70-71 0.21595636445245248 1101 72-73 0.19155256998600834 1101 74-75 0.15582306281155667 1101 76-77 0.29786800791586643 1101 78-79 0.33242165684366753 1101 80-81 0.3457175468843765 1101 82-83 0.2563514679589822 1101 84-85 0.2748706506657861 1101 86-87 0.23685321786908275 1101 88-89 0.2565910541068597 1101 90-91 0.16040411699391655 1101 92-93 0.11409713614861516 1101 94-95 0.1267095230480777 1101 96-97 0.12686071240376506 1101 98-99 0.1097212823789917 1101 100 0.14310277499105695 1102 1 0.34312564772088905 1102 2 0.2728836963266872 1102 3 0.3018347727217616 1102 4 0.19929059949924977 1102 5 0.187527379555128 1102 6 0.16507793006070415 1102 7 0.20574144672348638 1102 8 0.17970539255302498 1102 9 0.22500038958298774 1102 10-11 0.22787944745645206 1102 12-13 0.2758818291460088 1102 14-15 0.36904724525810906 1102 16-17 0.381331373657261 1102 18-19 0.3003814195136272 1102 20-21 0.3719319359082931 1102 22-23 0.3846654424755016 1102 24-25 0.3638616598395785 1102 26-27 0.3097207257830803 1102 28-29 0.3271115703541341 1102 30-31 0.2965040835646491 1102 32-33 0.40015717540364193 1102 34-35 0.454634026106703 1102 36-37 0.46264928050012344 1102 38-39 0.5126953116946069 1102 40-41 0.4236201852560697 1102 42-43 0.4276268254182156 1102 44-45 0.4720507469835482 1102 46-47 0.5704412909681835 1102 48-49 0.4561317302619585 1102 50-51 0.43138548760592954 1102 52-53 0.47602279698560324 1102 54-55 0.4583160426775734 1102 56-57 0.4346204586707998 1102 58-59 0.44612965539110405 1102 60-61 0.45944371981323684 1102 62-63 0.5325069077738718 1102 64-65 0.4315113534899737 1102 66-67 0.43308524370107904 1102 68-69 0.39530754549465996 1102 70-71 0.40492894904028276 1102 72-73 0.5789542121029356 1102 74-75 0.4667146491137828 1102 76-77 0.5333502572647077 1102 78-79 0.4518046955373869 1102 80-81 0.43835548168090455 1102 82-83 0.40276086065260586 1102 84-85 0.31845007906871814 1102 86-87 0.3616639710270739 1102 88-89 0.2894512516097656 1102 90-91 0.34579518979503376 1102 92-93 0.22122701406540557 1102 94-95 0.22005093525092434 1102 96-97 0.33791295603571214 1102 98-99 0.1692279483686363 1102 100 0.33760432542165475 1103 1 0.29855677872476605 1103 2 0.22969830837734406 1103 3 0.2729574486394313 1103 4 0.14434893921639969 1103 5 0.17482656328777324 1103 6 0.14860002101767833 1103 7 0.2296850770454384 1103 8 0.22269429099752358 1103 9 0.2717525053816985 1103 10-11 0.2806511948783026 1103 12-13 0.2600622580874017 1103 14-15 0.38851036662148175 1103 16-17 0.33668280559398056 1103 18-19 0.37168162637247804 1103 20-21 0.3479678647215678 1103 22-23 0.37031964423062647 1103 24-25 0.3376191090795615 1103 26-27 0.32662677618430536 1103 28-29 0.4268155433137082 1103 30-31 0.44851968143355236 1103 32-33 0.4752067580377357 1103 34-35 0.5145021716992773 1103 36-37 0.5255499502159537 1103 38-39 0.5464985796067978 1103 40-41 0.5709609167385068 1103 42-43 0.6254795734894287 1103 44-45 0.48780618652103414 1103 46-47 0.5940605410104567 1103 48-49 0.6209663562205279 1103 50-51 0.6011291699911752 1103 52-53 0.5329772732845584 1103 54-55 0.5562307292764714 1103 56-57 0.6104046534963388 1103 58-59 0.5631047038637078 1103 60-61 0.49929037677748767 1103 62-63 0.5711969975959121 1103 64-65 0.5244764742000498 1103 66-67 0.4690492193166165 1103 68-69 0.5769463221129669 1103 70-71 0.6327028928398164 1103 72-73 0.5329499815207726 1103 74-75 0.6162253611422059 1103 76-77 0.5937769054921809 1103 78-79 0.555023752704539 1103 80-81 0.5778446536404154 1103 82-83 0.5759601998305897 1103 84-85 0.44492241357657036 1103 86-87 0.42086863457653223 1103 88-89 0.6112254225600999 1103 90-91 0.48067907553032896 1103 92-93 0.5256314586404116 1103 94-95 0.6341087406628247 1103 96-97 0.6062652991476725 1103 98-99 0.5766278527703683 1103 100 0.6579411042364711 1104 1 0.21427468616438716 1104 2 0.2152752890462608 1104 3 0.2706991703173358 1104 4 0.13041255462359658 1104 5 0.14147038034186465 1104 6 0.2005909461888109 1104 7 0.21672597846265518 1104 8 0.16649164293225027 1104 9 0.25334684649475747 1104 10-11 0.25011666192150983 1104 12-13 0.25954303305568516 1104 14-15 0.31464237020507824 1104 16-17 0.319398675594158 1104 18-19 0.29594146733748516 1104 20-21 0.19928939583539318 1104 22-23 0.28656745222510693 1104 24-25 0.3327812649912474 1104 26-27 0.3638485554917068 1104 28-29 0.41533713626806446 1104 30-31 0.32919442664086773 1104 32-33 0.31527251785100674 1104 34-35 0.43793351455606455 1104 36-37 0.30925437365949193 1104 38-39 0.4474551980858692 1104 40-41 0.5508913560162014 1104 42-43 0.5414483028738672 1104 44-45 0.5130017247583538 1104 46-47 0.4571985374029026 1104 48-49 0.4242051901518167 1104 50-51 0.46138414094458824 1104 52-53 0.49266685021110845 1104 54-55 0.5183033084589042 1104 56-57 0.5937382592962663 1104 58-59 0.5863199501737455 1104 60-61 0.5862277855158524 1104 62-63 0.6076040195674679 1104 64-65 0.7299497127422541 1104 66-67 0.7246580968984517 1104 68-69 0.6555701055437453 1104 70-71 0.5897579056779207 1104 72-73 0.5979892145968293 1104 74-75 0.5971041955751986 1104 76-77 0.4861128100176302 1104 78-79 0.656400493537074 1104 80-81 0.7448806534774981 1104 82-83 0.610932086210326 1104 84-85 0.613520985860994 1104 86-87 0.6291129288793549 1104 88-89 0.6707909308820064 1104 90-91 0.5257873182884829 1104 92-93 0.5853784860885511 1104 94-95 0.5376725826166044 1104 96-97 0.5119691430829398 1104 98-99 0.5800186525974738 1104 100 0.6977033057252378 1105 1 0.2519942345141004 1105 2 0.15945274313175162 1105 3 0.21239283100723583 1105 4 0.14617135037730122 1105 5 0.09018852984373638 1105 6 0.11778742799459962 1105 7 0.17091197752684906 1105 8 0.1455388531350792 1105 9 0.17574307906663478 1105 10-11 0.16835498537508897 1105 12-13 0.18982593985878538 1105 14-15 0.2661492406211039 1105 16-17 0.2610784808181279 1105 18-19 0.2744136131311663 1105 20-21 0.2303553170315098 1105 22-23 0.21743355793669394 1105 24-25 0.2880339919078452 1105 26-27 0.2645638980013345 1105 28-29 0.3795336647712446 1105 30-31 0.37231703460641086 1105 32-33 0.4040168128852528 1105 34-35 0.3855262069080112 1105 36-37 0.4375489978602829 1105 38-39 0.4182557064795631 1105 40-41 0.3710072005835485 1105 42-43 0.398209977632483 1105 44-45 0.4507453440749387 1105 46-47 0.46442485118890175 1105 48-49 0.5572983108253808 1105 50-51 0.5752004647358504 1105 52-53 0.5485612657682424 1105 54-55 0.48283029429400415 1105 56-57 0.5507876200104391 1105 58-59 0.53802304974338 1105 60-61 0.615623649299927 1105 62-63 0.6091180912501883 1105 64-65 0.6036721940885101 1105 66-67 0.6258554487202304 1105 68-69 0.5827904716698669 1105 70-71 0.5813216253388305 1105 72-73 0.5284407111337472 1105 74-75 0.577170506583677 1105 76-77 0.5337915063752554 1105 78-79 0.7426571335233056 1105 80-81 0.7672649900144997 1105 82-83 0.7793197090135493 1105 84-85 0.6872784044350126 1105 86-87 0.6088536632555197 1105 88-89 0.6360259220870965 1105 90-91 0.7244489196113442 1105 92-93 0.7713641954000927 1105 94-95 0.8427887500610005 1105 96-97 0.7941095154363396 1105 98-99 0.8708943330617522 1105 100 0.9316331284875474 1106 1 0.2934904097654254 1106 2 0.22230628101764438 1106 3 0.23145826267563763 1106 4 0.1555033091321647 1106 5 0.166972638782795 1106 6 0.12405032019471918 1106 7 0.1732053941413838 1106 8 0.13859185523249806 1106 9 0.14598246477225985 1106 10-11 0.15333134853263886 1106 12-13 0.18867790507975002 1106 14-15 0.26326506654343973 1106 16-17 0.2557890597134005 1106 18-19 0.3077889160309937 1106 20-21 0.3035432372246021 1106 22-23 0.278015344913463 1106 24-25 0.3382402582665591 1106 26-27 0.3354093398110436 1106 28-29 0.3665731867320403 1106 30-31 0.3764101373600539 1106 32-33 0.35407782174308267 1106 34-35 0.41685181027138185 1106 36-37 0.44167961323528715 1106 38-39 0.3666915398230586 1106 40-41 0.3469377514743499 1106 42-43 0.35720659548321976 1106 44-45 0.44491703939605287 1106 46-47 0.48823471861778245 1106 48-49 0.46303149863590676 1106 50-51 0.4127872977880642 1106 52-53 0.5112879398949914 1106 54-55 0.4224045534551024 1106 56-57 0.4674951685367503 1106 58-59 0.5978083169575754 1106 60-61 0.5127713636033775 1106 62-63 0.6233298212576486 1106 64-65 0.5411669700005532 1106 66-67 0.5886101556168413 1106 68-69 0.5707959657390376 1106 70-71 0.6063989100051543 1106 72-73 0.5238195311292273 1106 74-75 0.5472497606184064 1106 76-77 0.5637337411830359 1106 78-79 0.6525821843315889 1106 80-81 0.5858366558348855 1106 82-83 0.5661647702143071 1106 84-85 0.52189967110235 1106 86-87 0.43246482521471563 1106 88-89 0.5817384204754319 1106 90-91 0.5636119725267754 1106 92-93 0.5393741534888932 1106 94-95 0.7132290343362762 1106 96-97 0.69285424868022 1106 98-99 0.6622343472143228 1106 100 0.8273535238044332 1107 1 0.1996143840475355 1107 2 0.13771416391885793 1107 3 0.20475773622722926 1107 4 0.13856764899613694 1107 5 0.11600494820779517 1107 6 0.09772612279486736 1107 7 0.10086748058013484 1107 8 0.08971705740851377 1107 9 0.16755259812757828 1107 10-11 0.13436529206941827 1107 12-13 0.16772331796308748 1107 14-15 0.2226927506999843 1107 16-17 0.2105811333827674 1107 18-19 0.07743322839441191 1107 20-21 0.13443752781999763 1107 22-23 0.26863798486049717 1107 24-25 0.2448085311227075 1107 26-27 0.22180738498972374 1107 28-29 0.22657165497437148 1107 30-31 0.1645285489806554 1107 32-33 0.22026428889505212 1107 34-35 0.18682907557571582 1107 36-37 0.3225771201664074 1107 38-39 0.29511683962642365 1107 40-41 0.3194758419518209 1107 42-43 0.22897006437769818 1107 44-45 0.2953129685456872 1107 46-47 0.25485912275160416 1107 48-49 0.26516558180786376 1107 50-51 0.2376650681459509 1107 52-53 0.24842464056004587 1107 54-55 0.350318473649466 1107 56-57 0.3103510649686143 1107 58-59 0.3969419853821492 1107 60-61 0.3376439807162015 1107 62-63 0.286719320223618 1107 64-65 0.45159523665149237 1107 66-67 0.5125447044289118 1107 68-69 0.3550671067492317 1107 70-71 0.43876137789066405 1107 72-73 0.4039110883012853 1107 74-75 0.4790538122062955 1107 76-77 0.3812638547925502 1107 78-79 0.5337787394050331 1107 80-81 0.4987397773153539 1107 82-83 0.5353424473517805 1107 84-85 0.48092691257737386 1107 86-87 0.40108700030350164 1107 88-89 0.5116657135184433 1107 90-91 0.3748215422094887 1107 92-93 0.44303604943527475 1107 94-95 0.6248416766324816 1107 96-97 0.6667108768728767 1107 98-99 0.7500643548091404 1107 100 0.9505624601710956 1108 1 0.2912889584990026 1108 2 0.20270913268091562 1108 3 0.23415731724349342 1108 4 0.17075849244100993 1108 5 0.10216971773907346 1108 6 0.09985601115713649 1108 7 0.12259586012429935 1108 8 0.10026393091228414 1108 9 0.18119950296929943 1108 10-11 0.14373305563060512 1108 12-13 0.20471575557197497 1108 14-15 0.214717851987956 1108 16-17 0.2687903148493689 1108 18-19 0.2771488673375089 1108 20-21 0.28091124217308305 1108 22-23 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2316 84-85 -0.17745709659007503 2316 86-87 -0.1995015618159819 2316 88-89 -0.0677090847910371 2316 90-91 -0.006009147319129937 2316 92-93 0.15194379536898595 2316 94-95 0.18097813497391968 2316 96-97 0.1555872144664292 2316 98-99 0.11111122514106953 2316 100 -0.08746813931150044 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 1.0 10 11.0 11 27.0 12 60.0 13 159.0 14 511.0 15 1459.0 16 3427.0 17 7047.0 18 12392.0 19 18613.0 20 25168.0 21 33882.0 22 42396.0 23 47363.0 24 49781.0 25 53175.0 26 61841.0 27 72758.0 28 88574.0 29 106854.0 30 129405.0 31 156418.0 32 191829.0 33 241446.0 34 319077.0 35 433350.0 36 629544.0 37 981892.0 38 1010764.0 39 169177.0 40 33.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.31989100482991 20.00965572828126 13.830214655840795 23.840238611048033 2 15.085332126926549 21.560092773227595 39.987799460801675 23.36677563904418 3 17.166209802798463 27.066443007937156 31.251309221831274 24.516037967433107 4 11.880780000360035 17.936452931097406 38.490690629751036 31.69207643879152 5 12.234026666579384 38.06811928566724 35.80998231310831 13.887871734645074 6 29.8648026658975 38.162674571077304 18.508717558469932 13.463805204555266 7 25.97925511948098 33.266720330739794 23.253249761938125 17.5007747878411 8 25.139928798483098 36.33548053454938 20.870292585742636 17.65429808122488 9 26.1890617731568 15.30277385354901 20.84929447753616 37.65886989575803 10-11 24.146357678889053 26.553487977236067 29.56828719211772 19.731867151757157 12-13 24.745148498445822 24.559127819025925 29.265337475081495 21.43038620744676 14-15 22.167114833274862 25.958338404826904 27.094857556661793 24.77968920523644 16-17 21.63261260730496 28.619289649546147 27.783710184346468 21.96438755880242 18-19 21.412447211637048 27.653727568269947 29.803260297668675 21.130564922424323 20-21 22.243845378679364 27.01569959809978 29.76603417399747 20.97442084922339 22-23 21.955053099816197 26.93441766127605 29.526423078142592 21.584106160765153 24-25 21.668910568403852 26.959581845680862 29.719013316021726 21.652494269893563 26-27 21.671123790485655 27.05488818595913 29.633518253001117 21.6404697705541 28-29 21.68047691697771 27.24944859236093 29.69947414265369 21.37060034800767 30-31 22.084132424645052 27.065133229617672 29.671710275317004 21.179024070420276 32-33 21.831983628635896 27.130675410826054 29.592414854965178 21.44492610557288 34-35 22.01634352852753 27.287244505148024 29.494033070631126 21.20237889569332 36-37 22.015436828745425 27.259490859384854 29.340724611013336 21.384347700856388 38-39 21.897043091397318 27.39937306242751 29.339633053215845 21.363950792959326 40-41 22.05595617021703 27.202040103654056 29.39463395703475 21.34736976909416 42-43 21.881640144362294 27.363330082934773 29.1250815625685 21.62994821013443 44-45 21.885338731943147 27.514886955696323 29.099579556529516 21.500194755831014 46-47 21.854243600940322 27.35132723321388 28.883230931603094 21.911198234242704 48-49 21.84712183485713 27.445934030413195 28.877242476999843 21.82970165772983 50-51 21.93089412598434 27.713114224199657 28.65573207725966 21.70025957255634 52-53 21.960350178919054 27.822772349334446 28.417628088941548 21.79924938280495 54-55 21.845196840471296 27.735416064892586 28.630630866530122 21.788756228105992 56-57 22.01546156655837 27.769960382049884 28.346201947521994 21.86837610386975 58-59 21.915250244692132 27.786582841775502 28.5072649705096 21.790901943022764 60-61 22.147069946035 27.80348830536681 28.483909464982695 21.565532283615504 62-63 21.990370412880285 27.825582678814182 28.51483663695053 21.669210271355002 64-65 21.947484311564843 27.620585714535583 28.46867309659551 21.963256877304065 66-67 21.84309928212804 27.643737549660703 28.38650763238916 22.126655535822096 68-69 21.760208142126523 27.737583713291876 28.454049192542925 22.048158952038683 70-71 21.78177028129802 27.564896082596295 28.456734547000394 22.19659908910529 72-73 21.729238538241866 27.886068887466376 28.150427398687288 22.234265175604467 74-75 21.778993606669808 27.884964039115868 28.050195390112815 22.28584696410151 76-77 21.664443158151958 27.73730638829015 28.229615005884977 22.368635447672915 78-79 21.604259432943707 27.72031529427216 28.29016205480754 22.38526321797659 80-81 21.65462309123948 27.894792562220594 27.971304086768978 22.479280259770945 82-83 21.743847746560714 27.933041304187707 28.014972461146893 22.30813848810469 84-85 21.702607947882576 27.99401585058941 27.913996675449816 22.3893795260782 86-87 21.58702195268173 27.909809650668155 28.044607178152486 22.458561218497625 88-89 21.458682976558705 27.774619444093485 28.079585916707632 22.687111662640174 90-91 21.73179852570401 27.812236173820093 27.971928382919188 22.48403691755671 92-93 21.529594841221808 27.88905115344314 27.934500689979625 22.646853315355425 94-95 21.377209500258925 27.858195383830342 27.928725854211173 22.83586926169956 96-97 21.366400989009605 27.7864004426972 27.942856540715056 22.904342027578135 98-99 21.29965546072601 27.741421735242554 27.83159999766754 23.127322806363896 100 20.458520340852917 28.277992202154717 28.06461130261991 23.198876154372456 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2341.0 1 1707.5 2 2252.5 3 3240.5 4 2247.0 5 1617.5 6 2302.0 7 4218.5 8 6492.5 9 7753.0 10 6799.5 11 5308.5 12 5363.0 13 5761.0 14 6149.5 15 6615.5 16 7051.5 17 7586.5 18 8228.0 19 8898.5 20 9899.0 21 11400.5 22 13684.0 23 17011.5 24 21399.0 25 26612.0 26 32978.0 27 40292.0 28 48415.0 29 57548.0 30 67126.0 31 76455.0 32 86134.0 33 95753.5 34 105268.5 35 114877.0 36 126841.5 37 137710.5 38 145392.0 39 152271.5 40 156580.0 41 160318.0 42 163946.0 43 168523.0 44 172729.0 45 175953.0 46 178735.5 47 180362.5 48 183618.5 49 185954.0 50 184512.5 51 177856.5 52 167708.5 53 158881.5 54 149125.0 55 135865.0 56 121502.0 57 107389.0 58 93498.0 59 79260.5 60 65044.5 61 52926.5 62 42679.0 63 33236.5 64 25032.0 65 18710.0 66 14051.0 67 10834.0 68 8271.0 69 6341.5 70 5039.0 71 3846.5 72 2956.5 73 2430.5 74 1946.0 75 1446.5 76 1036.5 77 716.0 78 482.0 79 352.5 80 273.0 81 188.0 82 123.0 83 95.0 84 65.5 85 42.5 86 32.0 87 22.0 88 16.5 89 13.5 90 11.5 91 5.5 92 4.0 93 3.0 94 1.5 95 2.5 96 2.0 97 1.0 98 2.0 99 2.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0029661844741722043 2 0.06965419403142314 3 7.159755627312217E-4 4 5.523240055355139E-4 5 0.00141149468081298 6 5.114111162365869E-4 7 0.0 8 0.0011455609003699547 9 2.045644464946348E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.341497841333679E-4 18-19 9.614528985247836E-4 20-21 0.003211661809965766 22-23 0.0 24-25 1.022822232473174E-5 26-27 1.2273866789678088E-4 28-29 7.568884520301487E-4 30-31 0.0014319511254624435 32-33 0.0029354998071980092 34-35 0.004101517152217428 36-37 0.0012376149012925405 38-39 0.0014217229031377116 40-41 0.005155024051664797 42-43 0.0064744647315551915 44-45 0.005380044942808895 46-47 0.0068324525129208015 48-49 0.008693988976021978 50-51 0.008162121415135929 52-53 0.003907180928047525 54-55 0.002270665356090446 56-57 0.002465001580260349 58-59 0.00305823847509479 60-61 0.00735409185148212 62-63 0.014043349251856678 64-65 0.004101517152217428 66-67 0.010555525439123156 68-69 0.01270345212731682 70-71 0.005605065833952993 72-73 0.0076916231881982685 74-75 0.0028127611393012285 76-77 0.0028536740286001553 78-79 0.0074972869640283655 80-81 0.005778945613473432 82-83 0.016262873496323466 84-85 0.01752094484226547 86-87 0.022604371337657143 88-89 0.02071215020758177 90-91 0.02497731891699491 92-93 0.03470435834781479 94-95 0.03875473438840856 96-97 0.02294190267437329 98-99 0.018277833294295616 100 0.0190449499686505 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4888435.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.56231028619622 #Duplication Level Percentage of deduplicated Percentage of total 1 77.25447254037617 51.422361722289445 2 15.117647972738613 20.125311503178256 3 4.047913130911742 8.083153494939461 4 1.4986186285540837 3.9900607261796304 5 0.6895848435941613 2.295018016398633 6 0.3747301031777393 1.4965740840777004 7 0.2234148542933606 1.040970619781399 8 0.15680301903928867 0.8349736965684372 9 0.10946506121203758 0.6557622632903798 >10 0.491189327104333 5.558614259954131 >50 0.023563725301269108 1.077038298785373 >100 0.011588118403755896 1.4365794325817087 >500 5.603751899928829E-4 0.27499948002751134 >1k 3.362250776027471E-4 0.5183188995226758 >5k 0.0 0.0 >10k+ 1.1207502586757441E-4 1.1902635024252346 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18817 0.38492891896895426 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17500 0.3579877813656109 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11549 0.2362514792566537 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.0456444649463478E-5 0.0 5 0.0 0.0 0.0 2.0456444649463478E-5 0.0 6 0.0 0.0 0.0 2.0456444649463478E-5 0.0 7 0.0 0.0 0.0 2.0456444649463478E-5 0.0 8 0.0 0.0 0.0 2.0456444649463478E-5 0.0 9 0.0 0.0 0.0 6.136933394839044E-5 8.182577859785391E-5 10-11 0.0 0.0 0.0 1.1251044557204914E-4 8.182577859785391E-5 12-13 0.0 1.0228222324731739E-5 0.0 1.5342333487097608E-4 8.182577859785391E-5 14-15 0.0 2.0456444649463478E-5 0.0 1.6365155719570782E-4 9.205400092258565E-5 16-17 0.0 2.0456444649463478E-5 0.0 1.7387977952043956E-4 1.022822232473174E-4 18-19 0.0 2.0456444649463478E-5 0.0 2.2502089114409828E-4 1.022822232473174E-4 20-21 0.0 2.0456444649463478E-5 0.0 2.6593378044302525E-4 1.022822232473174E-4 22-23 0.0 2.0456444649463478E-5 0.0 2.863902250924887E-4 1.022822232473174E-4 24-25 0.0 2.0456444649463478E-5 0.0 3.9890067066453784E-4 1.2273866789678088E-4 26-27 0.0 2.0456444649463478E-5 0.0 5.420957832107822E-4 1.2273866789678088E-4 28-29 0.0 1.022822232473174E-4 0.0 0.001217158456643077 1.2273866789678088E-4 30-31 0.0 1.022822232473174E-4 0.0 0.004889090271221771 1.2273866789678088E-4 32-33 0.0 1.022822232473174E-4 0.0 0.013368286578424383 1.4319511254624434E-4 34-35 0.0 1.022822232473174E-4 0.0 0.026583149821977792 1.5342333487097608E-4 36-37 0.0 1.2273866789678088E-4 0.0 0.047908993369043466 1.7387977952043956E-4 38-39 0.0 1.2273866789678088E-4 0.0 0.08133482392626679 1.841080018451713E-4 40-41 0.0 1.2273866789678088E-4 0.0 0.12095895721227755 1.841080018451713E-4 42-43 4.0912889298926956E-5 1.4319511254624434E-4 0.0 0.1607671985001335 2.045644464946348E-4 44-45 4.0912889298926956E-5 1.4319511254624434E-4 0.0 0.2082363783092135 2.2502089114409828E-4 46-47 4.0912889298926956E-5 1.4319511254624434E-4 0.0 0.2656167055509585 2.4547733579356176E-4 48-49 4.0912889298926956E-5 1.5342333487097608E-4 0.0 0.33020792953163947 3.4775955904087913E-4 50-51 4.0912889298926956E-5 1.841080018451713E-4 0.0 0.39945299467007334 3.4775955904087913E-4 52-53 4.0912889298926956E-5 1.841080018451713E-4 0.0 0.4724620456240085 3.682160036903426E-4 54-55 6.136933394839044E-5 1.9433622416990305E-4 0.0 0.5548708328943721 3.9890067066453784E-4 56-57 6.136933394839044E-5 2.045644464946348E-4 0.0 0.6505149398529386 4.193571153140013E-4 58-59 6.136933394839044E-5 2.045644464946348E-4 0.0 0.7619411938585662 4.5004178228819655E-4 60-61 6.136933394839044E-5 2.045644464946348E-4 0.0 0.8826546737350502 4.909546715871235E-4 62-63 6.136933394839044E-5 2.045644464946348E-4 0.0 1.0082265592157817 5.011828939118553E-4 64-65 6.136933394839044E-5 2.045644464946348E-4 0.0 1.1390966638607245 5.11411116236587E-4 66-67 6.136933394839044E-5 2.045644464946348E-4 0.0 1.2913642096090059 5.11411116236587E-4 68-69 6.136933394839044E-5 2.045644464946348E-4 0.0 1.4705319800713317 5.11411116236587E-4 70-71 6.136933394839044E-5 2.045644464946348E-4 0.0 1.6660649062532282 5.420957832107823E-4 72-73 7.159755627312217E-5 2.045644464946348E-4 0.0 1.8551642805928688 6.136933394839043E-4 74-75 8.182577859785391E-5 2.045644464946348E-4 0.0 2.0555658406013375 6.23921561808636E-4 76-77 8.182577859785391E-5 2.045644464946348E-4 0.0 2.2839927297795715 6.341497841333678E-4 78-79 8.182577859785391E-5 2.045644464946348E-4 0.0 2.544781714393257 6.341497841333678E-4 80-81 8.182577859785391E-5 2.045644464946348E-4 0.0 2.8132111810835165 6.443780064580996E-4 82-83 8.182577859785391E-5 2.2502089114409828E-4 0.0 3.0952850963549685 6.750626734322948E-4 84-85 8.182577859785391E-5 2.2502089114409828E-4 0.0 3.3902977128672056 6.750626734322948E-4 86-87 8.182577859785391E-5 2.2502089114409828E-4 0.0 3.704457561571341 6.750626734322948E-4 88 8.182577859785391E-5 2.2502089114409828E-4 0.0 3.960920008141665 6.750626734322948E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9445 0.0 48.339306 1 GTATCAA 15130 0.0 35.055267 1 TACCTGG 4820 0.0 34.045643 2 GTACCTG 4995 0.0 33.794193 1 ACCTGGG 5615 0.0 28.119686 3 TCAACGC 18940 0.0 27.664057 4 ATCAACG 18930 0.0 27.62902 3 CAACGCA 19240 0.0 27.134731 5 TATCAAC 19620 0.0 26.937077 2 AACGCAG 19915 0.0 26.309408 6 GTACATG 15435 0.0 24.583857 1 ACGCAGA 22570 0.0 23.193707 7 TACATGG 15920 0.0 22.771646 2 CGCAGAG 23095 0.0 22.605421 8 ACATGGG 15655 0.0 22.362698 3 CATGGGG 9010 0.0 21.8008 4 GAGTACT 16725 0.0 20.131468 12-13 ATGGGGG 5940 0.0 19.93566 5 GCAGAGT 25685 0.0 19.850079 9 ATAACGC 540 0.0 19.144758 3 >>END_MODULE