FastQCFastQC Report
Mon 6 Jun 2016
ERR1211545_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211545_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3842823
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148550.38656477282456153No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138080.35931917759418014No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85820.22332540426660297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73550.050.058321
TACCTGG34900.040.2849922
GTACCTG37050.038.3769951
GTATCAA124850.036.0513531
ACCTGGG43750.031.0388953
ATCAACG149700.029.3792383
TCAACGC149700.029.3156994
CAACGCA151450.028.8816265
AACGCAG156400.028.2034536
TATCAAC158050.027.638772
TATAACG3150.025.3767412
GTACATG137500.024.8249021
ACGCAGA178050.024.5903287
CGCAGAG180650.024.133028
TACATGG141200.023.41092
ACATGGG138250.022.9076883
TAACGCA3650.021.8842284
GCAGAGT199450.021.365579
CATGGGG60050.021.1264214
GAGTACT126400.020.31440712-13
Produced by FastQC (version 0.11.5)