FastQCFastQC Report
Mon 6 Jun 2016
ERR1211545_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211545_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3842823
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165340.4302566108301111No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156110.4062378100682753No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106810.27794670740754907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86550.048.407451
TACCTGG37250.039.6368682
GTACCTG40300.037.4542921
GTATCAA141100.035.2248841
ACCTGGG42850.033.6693533
TCAACGC175250.028.0224114
ATCAACG175350.027.952833
CAACGCA178050.027.7137035
TAACGCA3600.027.4134734
AACGCAG183500.027.0951276
TATAACG3650.027.0534322
TATCAAC184050.026.7234082
ACGCAGA204950.024.2135017
TATCACG2352.0008883E-1024.010952
GTACATG147800.023.9246181
CGCAGAG208100.023.82448
TACATGG148950.023.0766532
ACATGGG146400.022.502163
GCAGAGT228750.021.3036299
GAGTACT143200.021.15136712-13
Produced by FastQC (version 0.11.5)