##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211545_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3842823 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11055362164742 33.0 31.0 34.0 30.0 34.0 2 32.32547322632346 34.0 31.0 34.0 30.0 34.0 3 32.33587261239979 34.0 31.0 34.0 30.0 34.0 4 35.97629060719164 37.0 35.0 37.0 35.0 37.0 5 35.90791821533284 37.0 35.0 37.0 35.0 37.0 6 35.92620685365941 37.0 35.0 37.0 35.0 37.0 7 35.857668958471415 37.0 35.0 37.0 35.0 37.0 8 35.85339319557523 37.0 35.0 37.0 35.0 37.0 9 37.4957972303174 39.0 37.0 39.0 35.0 39.0 10-11 37.45862026432131 39.0 37.0 39.0 34.5 39.0 12-13 37.387756214637 39.0 37.0 39.0 34.0 39.0 14-15 38.61212395158455 40.0 38.0 41.0 34.0 41.0 16-17 38.544405896394395 40.0 38.0 41.0 34.0 41.0 18-19 38.55886661966996 40.0 38.0 41.0 34.0 41.0 20-21 38.51085074696388 40.0 38.0 41.0 34.0 41.0 22-23 38.39595487484071 40.0 38.0 41.0 34.0 41.0 24-25 38.29473384540479 40.0 38.0 41.0 34.0 41.0 26-27 38.120587260979754 40.0 38.0 41.0 33.0 41.0 28-29 38.14591044136043 40.0 38.0 41.0 33.5 41.0 30-31 38.05286751432476 40.0 37.5 41.0 33.0 41.0 32-33 37.847083511262426 40.0 37.0 41.0 33.0 41.0 34-35 37.717032244264175 40.0 37.0 41.0 33.0 41.0 36-37 37.63897296336573 40.0 37.0 41.0 33.0 41.0 38-39 37.537226669039924 40.0 37.0 41.0 32.0 41.0 40-41 37.37630720956963 40.0 36.5 41.0 31.5 41.0 42-43 37.224531028361184 40.0 36.0 41.0 31.0 41.0 44-45 37.033421914046 40.0 36.0 41.0 31.0 41.0 46-47 36.76145778767328 39.0 36.0 41.0 30.0 41.0 48-49 36.53073053325641 39.0 35.5 41.0 30.0 41.0 50-51 36.332196668959256 39.0 35.0 41.0 29.0 41.0 52-53 36.1465573616063 39.0 35.0 40.0 28.0 41.0 54-55 35.96684494706105 39.0 35.0 40.0 28.0 41.0 56-57 35.78207505263708 38.5 35.0 40.0 27.0 41.0 58-59 35.547283988880054 38.0 34.0 40.0 27.0 41.0 60-61 35.253649595622804 38.0 34.0 40.0 26.0 41.0 62-63 34.950314521381806 38.0 34.0 40.0 26.0 41.0 64-65 34.65554164217295 37.0 33.5 40.0 26.0 41.0 66-67 34.33262473967706 37.0 33.0 39.5 25.0 41.0 68-69 33.9492128573187 36.0 32.5 39.0 24.5 40.5 70-71 33.568892452241485 36.0 32.0 39.0 23.5 40.0 72-73 33.16049399100609 35.0 32.0 38.5 23.0 40.0 74-75 32.74803471302217 35.0 31.0 37.5 22.0 39.5 76-77 31.182745080894957 33.5 29.5 36.0 20.5 38.5 78-79 32.00045812674692 34.5 31.0 36.0 22.0 39.0 80-81 31.954693203408016 35.0 31.0 36.0 21.5 38.5 82-83 31.645396366161023 34.0 31.0 36.0 20.5 37.0 84-85 31.319684383069426 34.0 30.5 35.0 20.0 37.0 86-87 31.014995876729166 34.0 30.0 35.0 20.0 36.5 88-89 30.724774729411166 34.0 30.0 35.0 19.5 36.0 90-91 30.43323085658642 34.0 29.5 35.0 18.5 36.0 92-93 30.21260294840538 34.0 29.0 35.0 18.0 35.0 94-95 29.989859147819196 34.0 29.0 35.0 18.0 35.0 96-97 29.78001419789566 34.0 29.0 35.0 17.5 35.0 98-99 29.617052489797214 34.0 29.0 35.0 17.0 35.0 100 27.77673262598876 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3349350831094071 1101 2 0.2536597160442682 1101 3 0.26173827449768083 1101 4 0.19067751178925363 1101 5 0.20590190574865375 1101 6 0.18962520610961064 1101 7 0.19424985355186664 1101 8 0.17912075479779332 1101 9 0.19050959540463452 1101 10-11 0.1717111288048585 1101 12-13 0.2582656820503644 1101 14-15 0.34546033298114054 1101 16-17 0.2801628831490959 1101 18-19 0.3220396660980569 1101 20-21 0.274349426641578 1101 22-23 0.2808396003145077 1101 24-25 0.26848348891489593 1101 26-27 0.25911941145376716 1101 28-29 0.29863180490877284 1101 30-31 0.43516657642412326 1101 32-33 0.4488326997235106 1101 34-35 0.35217083442117314 1101 36-37 0.3608790667028927 1101 38-39 0.27073095920771806 1101 40-41 0.365082938290314 1101 42-43 0.32069812520843044 1101 44-45 0.3525656736257403 1101 46-47 0.4295168191475369 1101 48-49 0.3310881411623967 1101 50-51 0.3274646104072687 1101 52-53 0.25552281310712743 1101 54-55 0.2518200199784246 1101 56-57 0.35222252572135204 1101 58-59 0.3014832845062543 1101 60-61 0.19184048521417907 1101 62-63 0.18866864749077905 1101 64-65 0.2905775654885332 1101 66-67 0.28382791836011023 1101 68-69 0.25256919659014443 1101 70-71 0.28415364579000624 1101 72-73 0.1521586557339134 1101 74-75 0.1460844423830423 1101 76-77 0.2671192847817849 1101 78-79 0.3082659861546091 1101 80-81 0.2738289736541333 1101 82-83 0.4231065932050768 1101 84-85 0.2647698068167301 1101 86-87 0.24023222809479705 1101 88-89 0.2241898657912138 1101 90-91 0.132464224749544 1101 92-93 0.05095405385869256 1101 94-95 0.03128823454344598 1101 96-97 0.12277822925663884 1101 98-99 -0.010459348763699694 1101 100 0.11095818018248949 1102 1 0.2833515311214825 1102 2 0.23057990533528283 1102 3 0.3395650455998762 1102 4 0.20565740983040826 1102 5 0.23872518550917476 1102 6 0.22478797654271432 1102 7 0.20255516214173497 1102 8 0.20750312575916752 1102 9 0.25484947897471244 1102 10-11 0.2560191526665818 1102 12-13 0.2817971303833673 1102 14-15 0.3788017706358957 1102 16-17 0.44397955172838266 1102 18-19 0.34681729972697184 1102 20-21 0.35166134641268343 1102 22-23 0.4318402246714257 1102 24-25 0.3971167696673916 1102 26-27 0.39637231879802926 1102 28-29 0.41625364689784305 1102 30-31 0.39227325587616946 1102 32-33 0.4838814909801741 1102 34-35 0.410920875753213 1102 36-37 0.42018402713110703 1102 38-39 0.4218667636459017 1102 40-41 0.43512372571915137 1102 42-43 0.38810338731190797 1102 44-45 0.4253390437332385 1102 46-47 0.36406884791852434 1102 48-49 0.3621441601770812 1102 50-51 0.35846072227803205 1102 52-53 0.3324566176581243 1102 54-55 0.381033116302504 1102 56-57 0.345314337309496 1102 58-59 0.3915507578446622 1102 60-61 0.37513261617741733 1102 62-63 0.40218976767656756 1102 64-65 0.5446252850297384 1102 66-67 0.3675275346042781 1102 68-69 0.3245598837523147 1102 70-71 0.31913883433217904 1102 72-73 0.24763508285629143 1102 74-75 0.1771787786293899 1102 76-77 0.31158438984296666 1102 78-79 0.37389931942311705 1102 80-81 0.3541092146053444 1102 82-83 0.28632594087851615 1102 84-85 0.2104196340001785 1102 86-87 0.1598527902494311 1102 88-89 0.2933495513954121 1102 90-91 0.24667291556449555 1102 92-93 0.11873995973056495 1102 94-95 0.2517819238704462 1102 96-97 0.24691633467376306 1102 98-99 0.22893251592395458 1102 100 0.3278749462081372 1103 1 0.2999723669087828 1103 2 0.24800193088938016 1103 3 0.3052402270905432 1103 4 0.14904757499490273 1103 5 0.15689520645181432 1103 6 0.20370513005413926 1103 7 0.23139452706017494 1103 8 0.2471100189185904 1103 9 0.3086158605128375 1103 10-11 0.2650415894467386 1103 12-13 0.33751641197807203 1103 14-15 0.3724660158862392 1103 16-17 0.34239600785803503 1103 18-19 0.4025494850991649 1103 20-21 0.35711656279362103 1103 22-23 0.33667428787066456 1103 24-25 0.3840290651954774 1103 26-27 0.4091224065244603 1103 28-29 0.4201715804137294 1103 30-31 0.47380086283403955 1103 32-33 0.4993978019327088 1103 34-35 0.5252797371238884 1103 36-37 0.520093546613694 1103 38-39 0.4750863716869205 1103 40-41 0.560502605663558 1103 42-43 0.6395465881574722 1103 44-45 0.5633774923679269 1103 46-47 0.682786566807998 1103 48-49 0.6109270643282017 1103 50-51 0.6286353932028135 1103 52-53 0.6010161978654978 1103 54-55 0.6991146982208818 1103 56-57 0.7170673520748849 1103 58-59 0.7079808488374724 1103 60-61 0.688900291945167 1103 62-63 0.7018212589025765 1103 64-65 0.6357602358162708 1103 66-67 0.6796471861329536 1103 68-69 0.7325002906411555 1103 70-71 0.7445128223967643 1103 72-73 0.7225439764093693 1103 74-75 0.7225070526547839 1103 76-77 0.7048786240885114 1103 78-79 0.6987565400881124 1103 80-81 0.676525310199608 1103 82-83 0.5430404311273094 1103 84-85 0.5886412931640379 1103 86-87 0.6257602397349942 1103 88-89 0.6504415638030672 1103 90-91 0.6303174353687133 1103 92-93 0.5693236919575391 1103 94-95 0.6184131019391117 1103 96-97 0.5571687903396558 1103 98-99 0.6848944270542923 1103 100 0.8250189743206562 1104 1 0.26659762494648476 1104 2 0.2292916916540335 1104 3 0.29977793169175726 1104 4 0.07261987106873136 1104 5 0.16741837036499874 1104 6 0.18963685675856112 1104 7 0.22457672346660473 1104 8 0.19330293980202384 1104 9 0.2539891943685362 1104 10-11 0.3126194987579538 1104 12-13 0.2891139185054712 1104 14-15 0.40402765498719617 1104 16-17 0.34018904520038973 1104 18-19 0.3667566158889528 1104 20-21 0.2764994904605942 1104 22-23 0.374062556245633 1104 24-25 0.3791182820207126 1104 26-27 0.47101570452871755 1104 28-29 0.4552494311870987 1104 30-31 0.49757976611225985 1104 32-33 0.4067145079272976 1104 34-35 0.4838935905033068 1104 36-37 0.38179581704851273 1104 38-39 0.4822718786515523 1104 40-41 0.5002098467329361 1104 42-43 0.5419958575422683 1104 44-45 0.45167096723074707 1104 46-47 0.4532269279311478 1104 48-49 0.5064154407669079 1104 50-51 0.4390032804683912 1104 52-53 0.5621870131430029 1104 54-55 0.5600632866819666 1104 56-57 0.6563654849515856 1104 58-59 0.6840861548339561 1104 60-61 0.6214593474994672 1104 62-63 0.6127313037579896 1104 64-65 0.6635488962406484 1104 66-67 0.7502911504692946 1104 68-69 0.7171032081219835 1104 70-71 0.6408526247432818 1104 72-73 0.6808629132386272 1104 74-75 0.7435895597287256 1104 76-77 0.6652900463824025 1104 78-79 0.6683134308270056 1104 80-81 0.8236821581744636 1104 82-83 0.6563402423555829 1104 84-85 0.7555869902550398 1104 86-87 0.7441586121428685 1104 88-89 0.7482112545370327 1104 90-91 0.6364516881898652 1104 92-93 0.6375503571423877 1104 94-95 0.6673976329835298 1104 96-97 0.7118762786957653 1104 98-99 0.767956439826623 1104 100 0.8838176474017736 1105 1 0.2802203285558562 1105 2 0.19430747451325914 1105 3 0.20940815435693594 1105 4 0.12918376013302435 1105 5 0.0657741126153013 1105 6 0.14090740713092487 1105 7 0.10591827675891352 1105 8 0.13608081420981222 1105 9 0.09250593614445535 1105 10-11 0.1591197092796648 1105 12-13 0.19437396353683312 1105 14-15 0.23053559793593337 1105 16-17 0.2679871659379458 1105 18-19 0.23010893530931043 1105 20-21 0.19068693251583113 1105 22-23 0.23867048321432094 1105 24-25 0.2336225347415919 1105 26-27 0.34011514121502984 1105 28-29 0.3998506492723166 1105 30-31 0.3504482298235345 1105 32-33 0.3054928893196873 1105 34-35 0.39296103518464776 1105 36-37 0.37568350673745954 1105 38-39 0.4408775775323335 1105 40-41 0.445840364379265 1105 42-43 0.3192591026859688 1105 44-45 0.3336072130052088 1105 46-47 0.42230046828166934 1105 48-49 0.5430874789475908 1105 50-51 0.5074997155049488 1105 52-53 0.38059745000461476 1105 54-55 0.3633850616478256 1105 56-57 0.4057106678341924 1105 58-59 0.46177312193307074 1105 60-61 0.45081024535688385 1105 62-63 0.36882019110688447 1105 64-65 0.43312450863998464 1105 66-67 0.4875247242479759 1105 68-69 0.45333597742734355 1105 70-71 0.5621143712733172 1105 72-73 0.5144452746425117 1105 74-75 0.42242621243583756 1105 76-77 0.44441652832643186 1105 78-79 0.5916250569823944 1105 80-81 0.561970563112915 1105 82-83 0.35813208186395684 1105 84-85 0.4542467067038416 1105 86-87 0.3285739419529925 1105 88-89 0.3778601996146911 1105 90-91 0.5198535503096586 1105 92-93 0.5772795990978246 1105 94-95 0.6266112460671991 1105 96-97 0.6631022627508258 1105 98-99 0.4871321065717815 1105 100 0.7852739971445573 1106 1 0.18110379991368575 1106 2 0.15203621940869994 1106 3 0.22516053639138534 1106 4 0.1129606688270357 1106 5 0.16043823936784918 1106 6 0.13201839596861475 1106 7 0.08536237217846576 1106 8 0.10558705664623602 1106 9 0.14963542098259097 1106 10-11 0.17179417723868085 1106 12-13 0.16947631699390087 1106 14-15 0.2771571330608893 1106 16-17 0.2727812017529274 1106 18-19 0.27064852717674626 1106 20-21 0.30112171721113157 1106 22-23 0.249926126842702 1106 24-25 0.2976905867462847 1106 26-27 0.35132000293030075 1106 28-29 0.36596256725160003 1106 30-31 0.36378414615190735 1106 32-33 0.3491503790435999 1106 34-35 0.3674446584641018 1106 36-37 0.4138690221063541 1106 38-39 0.3766094950138452 1106 40-41 0.4178458023966982 1106 42-43 0.5325601580680512 1106 44-45 0.5782027618533405 1106 46-47 0.5675182406566037 1106 48-49 0.582810570197779 1106 50-51 0.6669436502081183 1106 52-53 0.6216151061503439 1106 54-55 0.5629822829508271 1106 56-57 0.6781366303160681 1106 58-59 0.8330611454485535 1106 60-61 0.848473445869665 1106 62-63 0.7476844854285858 1106 64-65 0.734213177989794 1106 66-67 0.782093163367108 1106 68-69 0.6801160034611655 1106 70-71 0.7287121661329152 1106 72-73 0.7448793941505443 1106 74-75 0.8322246160533879 1106 76-77 0.6624563453642196 1106 78-79 0.757777282407659 1106 80-81 0.7669273410083406 1106 82-83 0.8294990070793844 1106 84-85 0.7862005949410005 1106 86-87 0.6900347445103492 1106 88-89 0.8582928961130705 1106 90-91 0.7915900785656653 1106 92-93 0.8081685794561615 1106 94-95 0.8862204954290505 1106 96-97 0.8787834273374635 1106 98-99 0.8897096848727521 1106 100 0.8400953603253036 1107 1 0.23022210566915646 1107 2 0.17726289419289998 1107 3 0.24822157007189105 1107 4 0.19994073014338198 1107 5 0.18577363107492317 1107 6 0.16549233652219897 1107 7 0.24397383963948727 1107 8 0.20535005375436555 1107 9 0.21738345864213926 1107 10-11 0.22488608652527375 1107 12-13 0.2632403142977182 1107 14-15 0.3567944534061738 1107 16-17 0.3072792557200117 1107 18-19 0.28506656548858444 1107 20-21 0.3285662172219972 1107 22-23 0.373902557013821 1107 24-25 0.37955116816439016 1107 26-27 0.3601293351909547 1107 28-29 0.4537580054895116 1107 30-31 0.4003010733768022 1107 32-33 0.3951615551432752 1107 34-35 0.3581330540197527 1107 36-37 0.4068633035962179 1107 38-39 0.4014339171834038 1107 40-41 0.36079116127126554 1107 42-43 0.41136470678410575 1107 44-45 0.4339658459350062 1107 46-47 0.49585748094273896 1107 48-49 0.494831400360205 1107 50-51 0.557090635487846 1107 52-53 0.49866713806963503 1107 54-55 0.6026911390313643 1107 56-57 0.5491113862465795 1107 58-59 0.6759575817078272 1107 60-61 0.6363058478613581 1107 62-63 0.614862846412251 1107 64-65 0.634267536451226 1107 66-67 0.6789218907268122 1107 68-69 0.6283134768798817 1107 70-71 0.7004033665851779 1107 72-73 0.6579134660341737 1107 74-75 0.6787402386751964 1107 76-77 0.675721984293574 1107 78-79 0.7630826682440244 1107 80-81 0.7960776095391395 1107 82-83 0.7077567096733759 1107 84-85 0.7250908125922777 1107 86-87 0.6158169627704417 1107 88-89 0.5560989886094276 1107 90-91 0.4998036420889598 1107 92-93 0.5753980373935867 1107 94-95 0.7054753496396557 1107 96-97 0.8239476986079275 1107 98-99 0.9410403800087543 1107 100 1.1795596033293698 1108 1 0.2321862087594937 1108 2 0.1702350139599531 1108 3 0.1871173832520725 1108 4 0.11992834712771128 1108 5 0.0627864187869136 1108 6 0.16708345026722782 1108 7 0.13855517495287728 1108 8 0.15265951500094843 1108 9 0.17442500169963182 1108 10-11 0.16281304979245448 1108 12-13 0.16795929247986408 1108 14-15 0.11324255715600628 1108 16-17 0.2504658875973931 1108 18-19 0.2776786092972543 1108 20-21 0.26714213583076685 1108 22-23 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-0.18654134283271873 2316 84-85 -0.3057567714966183 2316 86-87 -0.327820569713527 2316 88-89 -0.27616211377738153 2316 90-91 -0.15611187748196897 2316 92-93 -0.08110996073383347 2316 94-95 -0.01516107879810491 2316 96-97 0.06458286735499996 2316 98-99 -0.15062861325008114 2316 100 -0.3685467727040468 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 2.0 10 6.0 11 19.0 12 53.0 13 141.0 14 388.0 15 1195.0 16 2931.0 17 6398.0 18 11432.0 19 16821.0 20 22190.0 21 29861.0 22 36467.0 23 39172.0 24 39112.0 25 41405.0 26 47047.0 27 56126.0 28 68465.0 29 82794.0 30 100061.0 31 120405.0 32 147557.0 33 185198.0 34 241095.0 35 327052.0 36 473502.0 37 757359.0 38 842062.0 39 146481.0 40 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.73315002429287 20.748116359082736 14.193386873938083 23.32534674268631 2 14.631408916828573 22.090153656979876 40.80202553163565 22.476411894555902 3 16.719956927139677 28.28212619421665 31.375632970931804 23.622283907711868 4 11.152516191352538 18.608631432562575 39.84647100337463 30.392381372710258 5 11.529669789765908 38.72504270363728 36.35240155263243 13.392885953964386 6 28.345250428138446 39.49353062702473 19.21547288838162 12.945746056455205 7 25.309050143605365 33.516870279999885 23.9940533300649 17.180026246329845 8 24.697049303018876 36.618240875697765 21.42296723705998 17.261742584223377 9 25.944188931988005 15.45132717570577 21.49373559326974 37.11074829903649 10-11 23.261375816684765 27.19616542317978 30.275984608190388 19.26647415194507 12-13 24.316602664239284 25.329399766786032 29.77753594167621 20.576461627298475 14-15 21.736116911968104 26.63173661654466 27.829579972848084 23.802566498639152 16-17 20.742511345578666 29.23233119422275 28.59132665495037 21.433830805248206 18-19 20.444752901248325 28.19406651763415 30.81547226614652 20.545708314971005 20-21 21.495383770624375 27.5488085502548 30.79801467330524 20.157793005815584 22-23 21.111784227376592 27.64854118964105 30.384680741215508 20.85499384176685 24-25 20.810391215000017 27.740530854530643 30.707269109193945 20.741808821275402 26-27 20.834106420312796 27.793346499015435 30.635023403392147 20.737523677279622 28-29 20.771501654461346 27.68147842525414 30.858101460878114 20.6889184594064 30-31 21.142845469723905 27.716323233669065 30.706615647887148 20.43421564871988 32-33 20.850301623308777 27.808456382440816 30.63188268062072 20.709359313629687 34-35 21.12505803265651 27.993886532884478 30.548975918789672 20.33207951566934 36-37 21.19157853702915 27.840794279865094 30.30893157590612 20.658695607199636 38-39 20.955868767445114 28.15296325615455 30.343520786849886 20.547647189550446 40-41 21.217289313459435 27.844836918900295 30.48292730912282 20.454946458517455 42-43 20.99576909389124 27.89515836783184 30.185429886215104 20.92364265206181 44-45 21.05601605570572 28.146266812903637 30.05212717411798 20.74558995727266 46-47 21.0317073050131 28.053458208644095 29.718288856393134 21.19654562994967 48-49 20.96833987223731 28.17095420199455 29.75774066695129 21.102965258816848 50-51 21.14183980400352 28.360075346426484 29.452614145203743 21.045470704366252 52-53 21.168538928180052 28.488093853272677 29.24631487563754 21.097052342909723 54-55 21.142996031740868 28.46691803224936 29.355736254024794 21.034349681984978 56-57 21.240401340299226 28.5182464314377 29.07360768038506 21.167744547878016 58-59 21.15746966482486 28.44402720228069 29.32268194761876 21.07582118527569 60-61 21.399060755515244 28.714737180832607 29.076163226227123 20.810038837425033 62-63 21.1575768901039 28.565102378730252 29.351844285014554 20.925476446151293 64-65 21.120894085849347 28.27009632746576 29.30030355168088 21.308706035004015 66-67 21.060383644296806 28.419172247214302 29.070906646675894 21.449537461812998 68-69 21.018635260327198 28.52360648053745 29.144864239265043 21.312894019870306 70-71 21.133711886158817 28.236476369681963 29.155693783129284 21.47411796102994 72-73 21.013729841634802 28.515546672548187 28.928033977503176 21.54268950831383 74-75 21.078971864482558 28.501338764204448 28.76293711516547 21.65675225614753 76-77 20.89056282067163 28.414043608739238 29.06007105391083 21.635322516678308 78-79 20.923730978222327 28.265782250864763 28.962951945934673 21.84753482497824 80-81 20.881129161517705 28.559191704270294 28.680919069583943 21.878760064628057 82-83 21.144312402725507 28.47474897324984 28.746063473720923 21.63487515030373 84-85 20.994145486762147 28.55726616562958 28.617583736665065 21.831004610943207 86-87 20.78814778568096 28.48744105419533 28.902005670249668 21.822405489874043 88-89 20.896115980505073 28.293920523682175 28.864452990285073 21.945510505527686 90-91 21.109559377496705 28.435624767126082 28.705813821378502 21.749002033998714 92-93 20.967718003630303 28.4284116345655 28.67353385466763 21.930336507136566 94-95 20.70335125650968 28.359853646917333 28.70835985364692 22.22843524292607 96-97 20.81479839763933 28.28962274820134 28.699752326711664 22.195826527447668 98-99 20.711626493041926 28.17179989568357 28.67023098489494 22.446342626379565 100 19.96327038302442 28.585724953157506 28.863695693874053 22.587308969944022 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1764.0 1 1254.5 2 1519.5 3 2128.5 4 1472.5 5 1141.5 6 1647.0 7 3039.0 8 4728.0 9 5719.5 10 5123.0 11 4113.5 12 4224.0 13 4692.5 14 5165.5 15 5457.5 16 5864.0 17 6424.0 18 7002.0 19 7683.0 20 8755.0 21 10280.0 22 12213.5 23 14856.5 24 18263.0 25 22650.0 26 28414.5 27 34866.5 28 41318.0 29 49308.5 30 58964.0 31 68478.5 32 78825.5 33 90983.0 34 103394.5 35 113528.0 36 125035.5 37 135893.5 38 141370.0 39 145264.0 40 144327.5 41 141642.5 42 140731.5 43 139960.0 44 141255.0 45 142915.5 46 141180.5 47 137541.5 48 135233.0 49 133102.0 50 130712.0 51 123726.0 52 113410.0 53 104907.5 54 96337.5 55 86835.0 56 76866.0 57 66830.5 58 57352.5 59 47828.5 60 38104.5 61 29625.5 62 22862.0 63 17215.0 64 12690.0 65 9311.0 66 6816.0 67 5063.0 68 3880.0 69 3000.5 70 2286.5 71 1775.5 72 1456.5 73 1139.0 74 852.5 75 635.0 76 466.0 77 305.5 78 211.5 79 151.5 80 102.0 81 71.0 82 46.0 83 30.5 84 23.0 85 14.5 86 10.0 87 6.5 88 4.0 89 4.0 90 3.0 91 2.5 92 3.0 93 3.0 94 2.5 95 2.5 96 3.0 97 2.0 98 1.5 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003434974756838918 2 0.0688556303529983 3 8.32721153173071E-4 4 8.066986171364124E-4 5 0.0015353296261628494 6 6.245408648798031E-4 7 0.0 8 9.368112973197048E-4 9 2.602253603665847E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.895972049714493E-4 18-19 9.758451013746925E-4 20-21 0.00339594095278393 22-23 0.0 24-25 0.0 26-27 1.0409014414663388E-4 28-29 8.197098851547417E-4 30-31 0.0014312394820162157 32-33 0.0030186141802523822 34-35 0.004137583229828696 36-37 0.0012100479257046188 38-39 0.0014832845540895326 40-41 0.005074394527148401 42-43 0.006908983317732822 44-45 0.0052435410113866815 46-47 0.007026084729897786 48-49 0.008027952367309138 50-51 0.007936873491180833 52-53 0.0037472451892788194 54-55 0.0020818028829326773 56-57 0.0025762310676291883 58-59 0.00339594095278393 60-61 0.0069350058537694815 62-63 0.013557741275099061 64-65 0.003890369137480441 66-67 0.009979642570058522 68-69 0.01187928770073459 70-71 0.005373653691569973 72-73 0.0072993213582827 74-75 0.0026022536036658466 76-77 0.002784411355922456 78-79 0.007455456574502651 80-81 0.005711946660046534 82-83 0.015691589230105055 84-85 0.01714885124815793 86-87 0.021871941538811442 88-89 0.020765983757253456 90-91 0.022990910588387755 92-93 0.03304862076655626 94-95 0.037160181460348295 96-97 0.022548527475764562 98-99 0.017357031536451198 100 0.01800759493736766 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3842823.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.60917324666688 #Duplication Level Percentage of deduplicated Percentage of total 1 80.65237531700478 56.14145166196587 2 13.829345966759629 19.252986785765298 3 3.184675973677094 6.650479847585592 4 1.0482443421552017 2.9186968807167917 5 0.42908971445731503 1.4934290136011033 6 0.2282389810282485 0.953251606322284 7 0.13170756272731937 0.6417638186247554 8 0.08107145324468144 0.4514653467414468 9 0.0630548404650956 0.3950275782578203 >10 0.29746182836461904 3.7609077318638335 >50 0.0287982264271905 1.406999694098038 >100 0.02352205289884727 3.13472518931309 >500 0.001832655045394654 0.8789234464843476 >1k 4.46989034234092E-4 0.5873802318148132 >5k 0.0 0.0 >10k+ 1.340967102702276E-4 1.332511166845068 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16534 0.4302566108301111 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15611 0.4062378100682753 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10681 0.27794670740754907 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.6022536036658467E-5 0.0 10-11 0.0 0.0 0.0 2.6022536036658467E-5 0.0 12-13 0.0 0.0 0.0 2.6022536036658467E-5 0.0 14-15 0.0 0.0 0.0 2.6022536036658467E-5 0.0 16-17 1.3011268018329233E-5 0.0 0.0 2.6022536036658467E-5 0.0 18-19 2.6022536036658467E-5 0.0 0.0 2.6022536036658467E-5 0.0 20-21 2.6022536036658467E-5 0.0 0.0 6.505634009164616E-5 0.0 22-23 2.6022536036658467E-5 0.0 0.0 1.561352162199508E-4 0.0 24-25 5.204507207331693E-5 0.0 0.0 4.0334930856820625E-4 0.0 26-27 5.204507207331693E-5 0.0 0.0 6.765859369531201E-4 0.0 28-29 5.204507207331693E-5 0.0 0.0 0.001613397234272825 0.0 30-31 1.0409014414663387E-4 0.0 0.0 0.005360642423551644 2.6022536036658467E-5 32-33 1.0409014414663387E-4 0.0 0.0 0.01341461732689744 5.204507207331693E-5 34-35 1.0409014414663387E-4 0.0 0.0 0.025371972635742007 5.204507207331693E-5 36-37 1.0409014414663387E-4 0.0 0.0 0.046567328237600326 5.204507207331693E-5 38-39 1.0409014414663387E-4 0.0 0.0 0.07905646447936843 5.204507207331693E-5 40-41 1.0409014414663387E-4 0.0 0.0 0.11809026853435611 5.204507207331693E-5 42-43 1.0409014414663387E-4 0.0 0.0 0.15459988659378796 5.204507207331693E-5 44-45 1.0409014414663387E-4 1.3011268018329233E-5 0.0 0.20116721483138827 5.204507207331693E-5 46-47 1.0409014414663387E-4 2.6022536036658467E-5 0.0 0.25750600535075385 5.204507207331693E-5 48-49 1.0409014414663387E-4 2.6022536036658467E-5 0.0 0.319634810138276 5.204507207331693E-5 50-51 1.0409014414663387E-4 2.6022536036658467E-5 0.0 0.3879699897705411 5.204507207331693E-5 52-53 1.0409014414663387E-4 2.6022536036658467E-5 0.0 0.4558107412181097 6.505634009164616E-5 54-55 1.0409014414663387E-4 2.6022536036658467E-5 0.0 0.5350883972537896 7.80676081099754E-5 56-57 1.0409014414663387E-4 2.6022536036658467E-5 0.0 0.6291208312222552 9.107887612830464E-5 58-59 1.0409014414663387E-4 2.6022536036658467E-5 0.0 0.7397816657181453 1.0409014414663387E-4 60-61 1.0409014414663387E-4 2.6022536036658467E-5 0.0 0.8539945763830392 1.0409014414663387E-4 62-63 1.0409014414663387E-4 2.6022536036658467E-5 0.0 0.9755718647463076 1.0409014414663387E-4 64-65 1.0409014414663387E-4 2.6022536036658467E-5 0.0 1.098853629219977 1.0409014414663387E-4 66-67 1.0409014414663387E-4 2.6022536036658467E-5 0.0 1.2433177380274865 1.4312394820162157E-4 68-69 1.0409014414663387E-4 2.6022536036658467E-5 0.0 1.4165627716915403 1.8215775225660926E-4 70-71 1.0409014414663387E-4 2.6022536036658467E-5 0.0 1.6028320846419417 1.8215775225660926E-4 72-73 1.561352162199508E-4 2.6022536036658467E-5 0.0 1.7915084821757339 2.342028243299262E-4 74-75 1.561352162199508E-4 5.204507207331693E-5 0.0 1.981368905099194 2.342028243299262E-4 76-77 1.561352162199508E-4 5.204507207331693E-5 0.0 2.1953001738565634 2.342028243299262E-4 78-79 1.561352162199508E-4 6.505634009164616E-5 0.0 2.4420718830921953 2.342028243299262E-4 80-81 1.561352162199508E-4 7.80676081099754E-5 0.0 2.7101040042697777 2.342028243299262E-4 82-83 1.561352162199508E-4 7.80676081099754E-5 0.0 2.9919931258868804 2.342028243299262E-4 84-85 1.561352162199508E-4 7.80676081099754E-5 0.0 3.276796771540089 2.342028243299262E-4 86-87 1.561352162199508E-4 7.80676081099754E-5 0.0 3.575626564117057 2.342028243299262E-4 88 1.561352162199508E-4 7.80676081099754E-5 0.0 3.817896374618347 2.342028243299262E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8655 0.0 48.40745 1 TACCTGG 3725 0.0 39.636868 2 GTACCTG 4030 0.0 37.454292 1 GTATCAA 14110 0.0 35.224884 1 ACCTGGG 4285 0.0 33.669353 3 TCAACGC 17525 0.0 28.022411 4 ATCAACG 17535 0.0 27.95283 3 CAACGCA 17805 0.0 27.713703 5 TAACGCA 360 0.0 27.413473 4 AACGCAG 18350 0.0 27.095127 6 TATAACG 365 0.0 27.053432 2 TATCAAC 18405 0.0 26.723408 2 ACGCAGA 20495 0.0 24.213501 7 TATCACG 235 2.0008883E-10 24.01095 2 GTACATG 14780 0.0 23.924618 1 CGCAGAG 20810 0.0 23.8244 8 TACATGG 14895 0.0 23.076653 2 ACATGGG 14640 0.0 22.50216 3 GCAGAGT 22875 0.0 21.303629 9 GAGTACT 14320 0.0 21.151367 12-13 >>END_MODULE