FastQCFastQC Report
Mon 6 Jun 2016
ERR1211544_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211544_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5613265
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198240.3531634440918075No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174530.31092421255721936No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110870.19751428090425094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97900.051.746171
GTATCAA165200.038.018451
TCAACGC201850.030.3787844
ATCAACG204000.030.0824533
TACCTGG48650.030.0607012
CAACGCA204000.030.0090715
TATCAAC213500.029.1836472
AACGCAG211800.029.0532466
GTACCTG53300.028.2664591
ACGCAGA237150.025.671467
GTACATG181950.025.5395771
CGCAGAG242650.024.9745358
TACATGG187550.024.2204172
ACATGGG183650.023.923293
ACCTGGG62550.022.53693
GCAGAGT267150.021.89469
GAGTACT168900.021.3370812-13
CATGGGG112450.020.9347064
CAGAGTA260050.020.18017610-11
AGAGTAC244600.019.72616410-11
Produced by FastQC (version 0.11.5)