FastQCFastQC Report
Mon 6 Jun 2016
ERR1211544_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211544_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5613265
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203130.36187495156562177No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188200.335277240607739No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128510.2289398416073355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104900.047.5238531
GTATCAA168450.035.2625431
TACCTGG51500.029.5874442
GTACCTG55500.028.2184091
TCAACGC210550.027.6080534
ATCAACG213700.027.2235773
CAACGCA215700.027.0575795
TATCAAC224650.026.147232
AACGCAG224850.026.0607646
GTACATG196800.025.1164881
ACCTGGG60900.024.0750053
TACATGG204250.023.6471062
ACATGGG200150.023.4317133
ACGCAGA254250.022.954847
CGCAGAG260950.022.3294478
CATGGGG117300.021.1122234
GAGTACT172250.019.67038512-13
GCAGAGT290000.019.6227469
AGAGTAC264100.019.29667710-11
AGTACTT186000.018.40574512-13
Produced by FastQC (version 0.11.5)