##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1211544_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5613265
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1594095415057	33.0	31.0	34.0	30.0	34.0
2	32.37487362524306	34.0	31.0	34.0	31.0	34.0
3	32.38735798149562	34.0	31.0	34.0	30.0	34.0
4	36.01428402186606	37.0	35.0	37.0	35.0	37.0
5	35.93715956756006	37.0	35.0	37.0	35.0	37.0
6	35.95703961954406	37.0	35.0	37.0	35.0	37.0
7	35.880707573934245	37.0	35.0	37.0	35.0	37.0
8	35.87783259831845	37.0	35.0	37.0	35.0	37.0
9	37.532008020287655	39.0	37.0	39.0	35.0	39.0
10-11	37.49669960352843	39.0	37.0	39.0	35.0	39.0
12-13	37.41410498168179	39.0	37.0	39.0	34.0	39.0
14-15	38.665763597478474	40.0	38.0	41.0	34.0	41.0
16-17	38.59426581499359	40.0	38.0	41.0	34.0	41.0
18-19	38.57594599577964	40.0	38.0	41.0	34.0	41.0
20-21	38.513610260694975	40.0	38.0	41.0	34.0	41.0
22-23	38.40266164166488	40.0	38.0	41.0	34.0	41.0
24-25	38.289909794032525	40.0	38.0	41.0	34.0	41.0
26-27	38.119006941592815	40.0	38.0	41.0	33.0	41.0
28-29	38.16053215018354	40.0	38.0	41.0	33.5	41.0
30-31	38.086292826011245	40.0	37.5	41.0	33.0	41.0
32-33	37.90491630094072	40.0	37.0	41.0	33.0	41.0
34-35	37.78549079724545	40.0	37.0	41.0	33.0	41.0
36-37	37.72749478244836	40.0	37.0	41.0	33.0	41.0
38-39	37.627211970216976	40.0	37.0	41.0	32.5	41.0
40-41	37.46611811485829	40.0	37.0	41.0	32.0	41.0
42-43	37.309546137586594	40.0	36.0	41.0	31.0	41.0
44-45	37.119891542622696	40.0	36.0	41.0	31.0	41.0
46-47	36.86127405351431	39.0	36.0	41.0	30.0	41.0
48-49	36.634776818838944	39.0	35.0	41.0	30.0	41.0
50-51	36.43021654954826	39.0	35.0	41.0	29.5	41.0
52-53	36.226143875266885	39.0	35.0	40.0	28.5	41.0
54-55	36.023188821479124	39.0	35.0	40.0	28.0	41.0
56-57	35.81670587438862	38.0	34.5	40.0	28.0	41.0
58-59	35.54889525080323	38.0	34.0	40.0	27.5	41.0
60-61	35.23093680772242	38.0	34.0	40.0	26.5	41.0
62-63	34.90390913309811	37.0	34.0	40.0	26.0	41.0
64-65	34.5781685881568	37.0	33.0	39.5	26.0	41.0
66-67	34.22690751282899	36.0	33.0	39.0	26.0	41.0
68-69	33.825153008097786	36.0	32.5	39.0	25.0	40.0
70-71	33.43669940756405	35.0	32.0	38.5	24.5	40.0
72-73	33.02834829640147	35.0	31.5	37.5	24.0	40.0
74-75	32.62236568200504	35.0	31.0	37.0	23.0	39.0
76-77	31.083028950174274	33.5	29.5	35.5	21.0	38.0
78-79	31.918835561834335	34.0	31.0	36.0	22.5	38.5
80-81	31.888319364932887	34.0	31.0	36.0	22.5	37.5
82-83	31.61424669955899	34.0	31.0	35.0	21.5	37.0
84-85	31.321033302365024	34.0	30.5	35.0	20.5	37.0
86-87	31.03069621690763	34.0	30.0	35.0	20.0	36.0
88-89	30.754619815740035	34.0	30.0	35.0	20.0	36.0
90-91	30.479554501702665	34.0	29.5	35.0	19.0	35.5
92-93	30.272883428806587	34.0	29.0	35.0	19.0	35.0
94-95	30.053500057453192	34.0	29.0	35.0	18.0	35.0
96-97	29.84456969339591	34.0	29.0	35.0	18.0	35.0
98-99	29.679270620574655	34.0	29.0	35.0	17.5	35.0
100	27.808110431273064	31.0	25.0	34.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.332114528173868
1101	2	0.249427584188048
1101	3	0.3233826922109202
1101	4	0.18988250731793954
1101	5	0.18158847072771067
1101	6	0.18741501100216595
1101	7	0.2108555015562672
1101	8	0.18701077303511227
1101	9	0.2576339171258013
1101	10-11	0.24927298731088854
1101	12-13	0.2396005533559773
1101	14-15	0.31505377601894935
1101	16-17	0.34838151041670784
1101	18-19	0.35485682171672295
1101	20-21	0.3433670743259398
1101	22-23	0.31949667103724977
1101	24-25	0.3411375037190325
1101	26-27	0.2992234329445651
1101	28-29	0.4036850826088667
1101	30-31	0.3969686529008385
1101	32-33	0.4330374911855017
1101	34-35	0.435794082979605
1101	36-37	0.43644651461934814
1101	38-39	0.4219189072616558
1101	40-41	0.46079184585362754
1101	42-43	0.4560565764108375
1101	44-45	0.44997334985868065
1101	46-47	0.4955437318754434
1101	48-49	0.5237483716619167
1101	50-51	0.463272990617817
1101	52-53	0.42780906613111114
1101	54-55	0.3799444099341045
1101	56-57	0.41551675555825796
1101	58-59	0.32949134953025805
1101	60-61	0.38857652730678893
1101	62-63	0.2545720679917238
1101	64-65	0.311925078121547
1101	66-67	0.23048072273002873
1101	68-69	0.25594936247158273
1101	70-71	0.27420766540169694
1101	72-73	0.22445114758607332
1101	74-75	0.2788385363595012
1101	76-77	0.2668633875725952
1101	78-79	0.36998420031376256
1101	80-81	0.3207018689587322
1101	82-83	0.2709947424362582
1101	84-85	0.27949937242713574
1101	86-87	0.21076795958714456
1101	88-89	0.16559432601335544
1101	90-91	0.11596643420684671
1101	92-93	0.08204656522371323
1101	94-95	0.10554014872298012
1101	96-97	0.0946335887094314
1101	98-99	-0.07106718704181247
1101	100	-0.14903683367973386
1102	1	0.31393136109274167
1102	2	0.25564101274472506
1102	3	0.2893322234235072
1102	4	0.21091705368728242
1102	5	0.1898011023223063
1102	6	0.12770677463190339
1102	7	0.17094924925771693
1102	8	0.18095535776192406
1102	9	0.2005181549608821
1102	10-11	0.21950684990476788
1102	12-13	0.23366767051292214
1102	14-15	0.3460911843311365
1102	16-17	0.3044990795376137
1102	18-19	0.2831474251152244
1102	20-21	0.3618535770278726
1102	22-23	0.2975683299390468
1102	24-25	0.29778129473107384
1102	26-27	0.2279468000526066
1102	28-29	0.29770974011470486
1102	30-31	0.3638242296400378
1102	32-33	0.4079076816347822
1102	34-35	0.33738366922444385
1102	36-37	0.3966547742685762
1102	38-39	0.3749807704157959
1102	40-41	0.4362444504759324
1102	42-43	0.3616857470616637
1102	44-45	0.367729465378666
1102	46-47	0.3533363738457993
1102	48-49	0.34157717524852416
1102	50-51	0.36128333880065355
1102	52-53	0.3985129338815767
1102	54-55	0.40620637903685264
1102	56-57	0.35008707875630307
1102	58-59	0.3685845009773061
1102	60-61	0.37830187371337587
1102	62-63	0.34244525516708535
1102	64-65	0.3422883013147384
1102	66-67	0.28373714126774985
1102	68-69	0.28432629364723994
1102	70-71	0.32536985774773086
1102	72-73	0.3002087359404868
1102	74-75	0.35601088400554914
1102	76-77	0.4084099605574174
1102	78-79	0.3302986335434319
1102	80-81	0.24835919658879035
1102	82-83	0.2098928983513062
1102	84-85	0.14562869448901594
1102	86-87	0.254913892654848
1102	88-89	0.16421023039511695
1102	90-91	0.1274429864244908
1102	92-93	0.03961723545434381
1102	94-95	0.06915366554408564
1102	96-97	0.017372600061744237
1102	98-99	-0.11280900497608215
1102	100	-0.06679979595294938
1103	1	0.259647818795095
1103	2	0.23762046502741896
1103	3	0.29466384368784304
1103	4	0.1473185006588551
1103	5	0.13968618416379996
1103	6	0.16209242767244803
1103	7	0.16909842272239217
1103	8	0.20467296736916296
1103	9	0.2659681534556455
1103	10-11	0.25052543160009577
1103	12-13	0.26593268023360395
1103	14-15	0.2953500812911116
1103	16-17	0.3304030381807408
1103	18-19	0.33955418548573846
1103	20-21	0.3112247356944522
1103	22-23	0.3305878289396489
1103	24-25	0.3123034587000646
1103	26-27	0.358477208578762
1103	28-29	0.3865964649457041
1103	30-31	0.4954936197681903
1103	32-33	0.5008751671587319
1103	34-35	0.46831747222573483
1103	36-37	0.4928221331146716
1103	38-39	0.4997321210718013
1103	40-41	0.5196257097004846
1103	42-43	0.522121466075312
1103	44-45	0.4815491451269196
1103	46-47	0.5969444009749836
1103	48-49	0.5569879062842773
1103	50-51	0.5830532146064513
1103	52-53	0.5793699473503082
1103	54-55	0.5607420267892564
1103	56-57	0.5414127903586206
1103	58-59	0.572070424406526
1103	60-61	0.5374621739731467
1103	62-63	0.6288926664311916
1103	64-65	0.5706789316499297
1103	66-67	0.6130774989564003
1103	68-69	0.6167645495848149
1103	70-71	0.6337853463328642
1103	72-73	0.5888905748013471
1103	74-75	0.5435959746408159
1103	76-77	0.5347764488201072
1103	78-79	0.544054194514775
1103	80-81	0.5032388362937006
1103	82-83	0.5029590340510879
1103	84-85	0.4524193901437066
1103	86-87	0.46300893342309024
1103	88-89	0.4101091316291807
1103	90-91	0.5203814267539961
1103	92-93	0.498492785362167
1103	94-95	0.5675658846880438
1103	96-97	0.6665053514546848
1103	98-99	0.5530207844408146
1103	100	0.51858787668424
1104	1	0.24094258422083215
1104	2	0.2005880789086092
1104	3	0.2675670377761534
1104	4	0.08880167909648407
1104	5	0.14299835454255572
1104	6	0.17073285901167168
1104	7	0.20209218665456063
1104	8	0.21141792635015833
1104	9	0.2308483989834258
1104	10-11	0.2526098699924404
1104	12-13	0.28472422703475786
1104	14-15	0.3191375725120693
1104	16-17	0.3057789939115878
1104	18-19	0.2505676885373447
1104	20-21	0.22527425025516123
1104	22-23	0.2957595433472946
1104	24-25	0.3198249383802221
1104	26-27	0.31923212970918513
1104	28-29	0.4507002702871148
1104	30-31	0.3908925478108429
1104	32-33	0.4449147776398661
1104	34-35	0.49566542772168276
1104	36-37	0.4084162478108837
1104	38-39	0.5118583788136704
1104	40-41	0.6034707982617817
1104	42-43	0.6248371297731765
1104	44-45	0.5963904249803775
1104	46-47	0.5723340313000094
1104	48-49	0.5444736146350451
1104	50-51	0.5429495045720572
1104	52-53	0.6033418883410704
1104	54-55	0.5912903279467443
1104	56-57	0.6455135508069532
1104	58-59	0.6254727464658387
1104	60-61	0.5571910280310846
1104	62-63	0.6392404343826641
1104	64-65	0.7004979429236826
1104	66-67	0.6436994818968458
1104	68-69	0.6179260220610985
1104	70-71	0.44963097040486133
1104	72-73	0.5530150442535202
1104	74-75	0.6081425625867709
1104	76-77	0.5082557874014988
1104	78-79	0.641082900192675
1104	80-81	0.7273030751340848
1104	82-83	0.579475961689063
1104	84-85	0.5829589531474539
1104	86-87	0.6368709820036003
1104	88-89	0.673828146219055
1104	90-91	0.5436298345255146
1104	92-93	0.6160938172140824
1104	94-95	0.5942014026336189
1104	96-97	0.5104323018984083
1104	98-99	0.5892404820055894
1104	100	0.6347490630885062
1105	1	0.24033631589885118
1105	2	0.12388494894537416
1105	3	0.2071308194509598
1105	4	0.12555411731334942
1105	5	0.09591242779490017
1105	6	0.13303405759845788
1105	7	0.16945835956466482
1105	8	0.11321919801370228
1105	9	0.12891502604445293
1105	10-11	0.1623191345638304
1105	12-13	0.18386093585728958
1105	14-15	0.23916153810481688
1105	16-17	0.2269822980560221
1105	18-19	0.26835233146331916
1105	20-21	0.23616021698386191
1105	22-23	0.28959640838258593
1105	24-25	0.29517454859059455
1105	26-27	0.30075791607779934
1105	28-29	0.3318173899315511
1105	30-31	0.38402823432665656
1105	32-33	0.3456663804012692
1105	34-35	0.43137400734246256
1105	36-37	0.3790772993841287
1105	38-39	0.40063023113156504
1105	40-41	0.4482894514430811
1105	42-43	0.4527915744141424
1105	44-45	0.4602904559772085
1105	46-47	0.4611462230114398
1105	48-49	0.49555366606265494
1105	50-51	0.5602779232908617
1105	52-53	0.5597633771564929
1105	54-55	0.5880072362700943
1105	56-57	0.6218112519478467
1105	58-59	0.4978264656120217
1105	60-61	0.5617158486602776
1105	62-63	0.5309102344339109
1105	64-65	0.5828924724309488
1105	66-67	0.5879923663708126
1105	68-69	0.5980599201800629
1105	70-71	0.5962450209534964
1105	72-73	0.5946176162482004
1105	74-75	0.633645825956684
1105	76-77	0.5955096908649331
1105	78-79	0.6756282411846932
1105	80-81	0.6458784060079772
1105	82-83	0.6155266454750574
1105	84-85	0.6184496078972757
1105	86-87	0.5665349364262298
1105	88-89	0.7087907286159769
1105	90-91	0.6647827400240836
1105	92-93	0.60765016272099
1105	94-95	0.6727752719723767
1105	96-97	0.6644630417448987
1105	98-99	0.7941238650678066
1105	100	0.7390328456028641
1106	1	0.28045600537935655
1106	2	0.17837081837062385
1106	3	0.20308001644265516
1106	4	0.12784145111159972
1106	5	0.16925066532012778
1106	6	0.14194804257735427
1106	7	0.17920550470027052
1106	8	0.19152694582945173
1106	9	0.12557792319793748
1106	10-11	0.1632878824522379
1106	12-13	0.1875483866931944
1106	14-15	0.25172957101815285
1106	16-17	0.27033429196701064
1106	18-19	0.2747084446405097
1106	20-21	0.2693586850401104
1106	22-23	0.30216628992977945
1106	24-25	0.23412405283799131
1106	26-27	0.27077313862144337
1106	28-29	0.35029837386374396
1106	30-31	0.3786697459736885
1106	32-33	0.4001972544991048
1106	34-35	0.417574593040662
1106	36-37	0.4290597692354581
1106	38-39	0.40079463205307775
1106	40-41	0.4266019164624595
1106	42-43	0.4569049122436937
1106	44-45	0.49409030375176854
1106	46-47	0.48866893889681506
1106	48-49	0.4973757178422531
1106	50-51	0.5034011965712253
1106	52-53	0.6293411109353642
1106	54-55	0.4504742955271013
1106	56-57	0.5250038426233345
1106	58-59	0.6009945140793818
1106	60-61	0.6009517636639217
1106	62-63	0.6113673697143795
1106	64-65	0.6764325170276493
1106	66-67	0.5904968190285729
1106	68-69	0.555993428007433
1106	70-71	0.6708534611782397
1106	72-73	0.6548668885876694
1106	74-75	0.5819216880256519
1106	76-77	0.6241608383565875
1106	78-79	0.6781136975358955
1106	80-81	0.5981189502457873
1106	82-83	0.5878270344531131
1106	84-85	0.6729414057000795
1106	86-87	0.5966744891588291
1106	88-89	0.6930557946631986
1106	90-91	0.7078904592816251
1106	92-93	0.7124885474994649
1106	94-95	0.6776811000137286
1106	96-97	0.7741167989975999
1106	98-99	0.795734407635468
1106	100	0.9164300456237733
1107	1	0.20649302247158374
1107	2	0.1852921483743657
1107	3	0.2497382348874524
1107	4	0.17921953517312517
1107	5	0.15113437031953225
1107	6	0.12179440660746366
1107	7	0.20671719062177374
1107	8	0.12332344821418673
1107	9	0.2051831787459406
1107	10-11	0.2283081954562931
1107	12-13	0.2161687089508746
1107	14-15	0.28959838281344474
1107	16-17	0.22071581143882923
1107	18-19	0.24124423241363502
1107	20-21	0.27349859222105266
1107	22-23	0.37172846468715903
1107	24-25	0.3533957021562415
1107	26-27	0.38128327070416645
1107	28-29	0.4023626403271692
1107	30-31	0.3015493472450359
1107	32-33	0.3401325819041219
1107	34-35	0.35841131401671333
1107	36-37	0.43851145307489503
1107	38-39	0.44935749183615314
1107	40-41	0.40210948451841233
1107	42-43	0.3460563800365648
1107	44-45	0.41785626144468324
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2316	82-83	-0.09952665643659486
2316	84-85	-0.06151906871413004
2316	86-87	-0.01674602743419129
2316	88-89	-0.10123447589937484
2316	90-91	0.006161490632628386
2316	92-93	0.1211829205362065
2316	94-95	0.22532854316678907
2316	96-97	0.23950965685575554
2316	98-99	0.10498825079545782
2316	100	-0.07685550093341931
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	8.0
11	29.0
12	60.0
13	180.0
14	570.0
15	1422.0
16	3373.0
17	6799.0
18	12428.0
19	18990.0
20	26362.0
21	35895.0
22	46349.0
23	53233.0
24	57351.0
25	62120.0
26	72346.0
27	85681.0
28	103286.0
29	123923.0
30	150641.0
31	181308.0
32	221708.0
33	277720.0
34	364764.0
35	501716.0
36	738414.0
37	1148474.0
38	1131864.0
39	186223.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.57048124481903	19.629708347756466	13.697181712244364	24.10262869518014
2	15.15055138649753	21.069760045524283	39.48534526681872	24.294343301159465
3	17.283968432373655	26.51102691699718	30.852386126392005	25.352618524237165
4	12.177670159068759	17.286009684614907	37.643956700176176	32.89236345614016
5	12.65793400999041	38.06658361218581	35.381689854045376	13.893792523778398
6	31.089933731464747	37.58664809622709	17.69598636932585	13.627431802982315
7	26.924276691016725	32.80634354515598	22.586106303550608	17.683273460276684
8	25.889517762563667	35.93945353906232	20.270059377789178	17.900969320584835
9	26.444997578947856	15.041592571307023	20.261783611739485	38.251626238005635
10-11	24.610480709533576	26.203849274887254	29.025593838879864	20.160076176699302
12-13	25.10867561036224	24.110531036749556	29.00426044378806	21.77653290910014
14-15	22.50722173280613	25.491385138595806	26.417325745354976	25.584067383243088
16-17	22.13058351901825	28.3442547090778	27.121642584413337	22.40351918749062
18-19	22.012119288085355	27.38473110084602	29.05512380547214	21.548025805596485
20-21	22.74839092997144	26.599986371173518	29.093758754628123	21.557863944226924
22-23	22.591470613935975	26.4840806984955	28.8883500857656	22.036098601802927
24-25	22.294541999093575	26.727328342297813	28.9043059439214	22.07382371468721
26-27	22.258399495194766	26.81164971333918	28.89724926041116	22.032701531054894
28-29	22.276890911024662	26.70493890569152	29.099033760914217	21.9191364223696
30-31	22.43642241360113	26.69957551698404	28.980385631605323	21.883616437809504
32-33	22.266822255081863	26.86612567030696	28.88564251483138	21.981409559779802
34-35	22.505214200440868	26.854072046915068	28.858227729480497	21.782486023163575
36-37	22.553265289561633	26.832036375682645	28.698369138960917	21.916329195794805
38-39	22.433209511815658	26.932730015942813	28.726586162192707	21.90747431004882
40-41	22.44211175188893	26.875254989782594	28.74983297612494	21.93280028220354
42-43	22.31628830596957	26.944414453995062	28.636392466066553	22.102904773968813
44-45	22.421350259840086	27.130228614201947	28.450206811133818	21.998214314824153
46-47	22.42719515571501	27.005832297917543	28.307846129670995	22.259126416696446
48-49	22.401135122355438	27.121027396906385	28.250697915313726	22.22713956542445
50-51	22.38258419879746	27.36371835173536	28.048057400350768	22.20564004911641
52-53	22.44467385009822	27.37557111273886	27.918867005679882	22.26088803148303
54-55	22.376529600952903	27.3925304290089	27.954122705142055	22.276817264896145
56-57	22.4460711445438	27.403442567577198	27.826744265594133	22.32374202228487
58-59	22.428982635082686	27.28746633544144	27.973967678104376	22.309583351371497
60-61	22.43322277473313	27.43138725788749	27.950017642519352	22.185372324860026
62-63	22.334377168996877	27.49459752460301	27.94246600897091	22.2285592974292
64-65	22.311143131783922	27.300257320430877	27.94581653205602	22.442783015729177
66-67	22.278023302531135	27.312627890879515	27.924553178107843	22.484795628481503
68-69	22.219777306374247	27.343665646691328	27.918029830982473	22.518527215951952
70-71	22.247599076776986	27.233609094690937	27.852713682389545	22.66607814614253
72-73	22.17862371627872	27.481160358874064	27.657577524360473	22.68263840048674
74-75	22.194190549064285	27.50176150590717	27.5921839911849	22.711863953843647
76-77	22.10582472088027	27.432807544788584	27.71297393433531	22.74839379999583
78-79	22.11960757938696	27.32131865155968	27.688823245923867	22.870250523129496
80-81	22.086067183042683	27.536681154555776	27.481362788002915	22.895888874398622
82-83	22.120159626509462	27.497620872251215	27.52163041833984	22.860589082899484
84-85	22.109401170290862	27.4730145324182	27.478190681861918	22.939393615429022
86-87	21.96212209699367	27.432742181026015	27.6276368581606	22.97749886381972
88-89	21.888968489097657	27.356288188314	27.639338650856942	23.1154046717314
90-91	22.09838593578865	27.406357520047393	27.493101922266998	23.002154621896956
92-93	21.939648650830524	27.361935409022546	27.465448989822576	23.232966950324354
94-95	21.764546116054554	27.25731879296478	27.551626345961612	23.42650874501906
96-97	21.780281819642163	27.233976660878174	27.582426814998982	23.403314704480678
98-99	21.724978179454197	27.173918369554208	27.497148428622246	23.603955022369345
100	21.000672990556573	27.572995064557738	27.650646450460357	23.775685494425332
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2547.0
1	1860.5
2	2215.0
3	3118.0
4	2288.0
5	1792.5
6	2657.0
7	5117.5
8	7968.0
9	9155.0
10	7760.0
11	6006.0
12	6053.0
13	6534.5
14	6871.0
15	7140.0
16	7459.5
17	8014.0
18	8589.5
19	9192.0
20	10130.5
21	11463.0
22	13537.0
23	16560.5
24	20431.5
25	25265.0
26	31423.0
27	38372.0
28	46297.0
29	55141.5
30	64971.5
31	74962.5
32	85541.5
33	97227.0
34	108767.5
35	119265.0
36	132703.0
37	146190.5
38	154900.5
39	162446.0
40	167988.5
41	173126.0
42	179452.5
43	186638.5
44	192581.5
45	198754.0
46	204451.5
47	209116.0
48	214962.0
49	218057.5
50	217667.0
51	211685.5
52	202926.0
53	196259.0
54	188720.0
55	175784.5
56	158776.5
57	142686.0
58	127239.0
59	109972.5
60	91294.5
61	74326.5
62	59776.5
63	46302.5
64	34487.5
65	25600.0
66	19065.5
67	14235.5
68	10728.5
69	8099.0
70	6258.0
71	4872.5
72	3777.0
73	2926.0
74	2285.0
75	1740.0
76	1229.0
77	836.0
78	551.0
79	373.0
80	265.5
81	204.0
82	161.5
83	105.0
84	73.5
85	50.5
86	34.5
87	25.0
88	13.5
89	11.0
90	10.0
91	6.0
92	5.0
93	5.5
94	4.5
95	4.0
96	4.5
97	3.0
98	1.0
99	3.0
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0031354300928247646
2	0.07038684259517411
3	8.194874106246543E-4
4	5.700781986954117E-4
5	0.0014608253841569925
6	7.660425794969595E-4
7	0.0
8	9.798219040077388E-4
9	5.344483112769485E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	6.680603890961856E-4
18-19	0.0011401563973908234
20-21	0.0028949283527501374
22-23	8.907471854615808E-6
24-25	1.7814943709231616E-5
26-27	1.959643808015478E-4
28-29	0.0010332667351354338
30-31	0.0014964552715754555
32-33	0.0029394657120232165
34-35	0.004061807165704808
36-37	0.0011490638692454392
38-39	0.001389565609320066
40-41	0.005255408394223326
42-43	0.006538084341288003
44-45	0.005228685978659479
46-47	0.006823123440635708
48-49	0.008515543093012713
50-51	0.008497728149303479
52-53	0.0037322307070840233
54-55	0.0021021633576893306
56-57	0.0022981277384908783
58-59	0.002957280655732448
60-61	0.007206144730384189
62-63	0.014100527945856822
64-65	0.003999454862722497
66-67	0.01015451791426202
68-69	0.012666424977263679
70-71	0.005700781986954117
72-73	0.007704963154242674
74-75	0.0030285404305693746
76-77	0.003064170317987838
78-79	0.007304126920784962
80-81	0.0053622980564787155
82-83	0.016282858550237696
84-85	0.01754771955359314
86-87	0.022437921601777218
88-89	0.02122650542954947
90-91	0.024504455072048088
92-93	0.03427595169656163
94-95	0.03791020021324488
96-97	0.022651700926287998
98-99	0.017966370730760083
100	0.017850573596650078
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5613265.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.138292845909035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.30000790882573	45.42575641983146
2	15.742521150675756	19.249417364858626
3	4.829496737031979	8.8580155742107
4	1.9856628569967103	4.85600148977237
5	1.0002764823317978	3.0577598251838607
6	0.5499361916072104	2.017329595742735
7	0.38028614274159256	1.627503189212366
8	0.23684884567577155	1.1584427269712672
9	0.1710259059076001	0.9410608727654167
>10	0.752972205010206	7.7713362385067235
>50	0.0347152104918342	1.4400430115465384
>100	0.015255567175450211	1.6673248847546698
>500	4.973978006312767E-4	0.21427390193256077
>1k	3.8261363671588484E-4	0.49970726466938903
>5k	0.0	0.0
>10k+	1.147840910146693E-4	1.2160276400413366
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20313	0.36187495156562177	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18820	0.335277240607739	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12851	0.2289398416073355	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	1.7814943709231616E-5	0.0	0.0	0.0
4	0.0	1.7814943709231616E-5	0.0	0.0	0.0
5	0.0	1.7814943709231616E-5	0.0	0.0	1.7814943709231616E-5
6	0.0	1.7814943709231616E-5	0.0	0.0	1.7814943709231616E-5
7	0.0	1.7814943709231616E-5	0.0	0.0	1.7814943709231616E-5
8	0.0	1.7814943709231616E-5	0.0	0.0	1.7814943709231616E-5
9	0.0	1.7814943709231616E-5	0.0	0.0	3.562988741846323E-5
10-11	0.0	1.7814943709231616E-5	0.0	0.0	3.562988741846323E-5
12-13	0.0	4.453735927307904E-5	0.0	0.0	3.562988741846323E-5
14-15	0.0	8.016724669154227E-5	0.0	0.0	4.453735927307904E-5
16-17	0.0	8.907471854615807E-5	0.0	0.0	5.3444831127694843E-5
18-19	0.0	8.907471854615807E-5	0.0	0.0	7.125977483692646E-5
20-21	0.0	8.907471854615807E-5	0.0	3.562988741846323E-5	7.125977483692646E-5
22-23	0.0	8.907471854615807E-5	0.0	1.6924196523770036E-4	8.016724669154227E-5
24-25	0.0	8.907471854615807E-5	0.0	2.672241556384742E-4	8.907471854615807E-5
26-27	0.0	8.907471854615807E-5	0.0	6.235230298231065E-4	8.907471854615807E-5
28-29	0.0	8.907471854615807E-5	0.0	0.001950736336160862	9.798219040077388E-5
30-31	0.0	8.907471854615807E-5	0.0	0.006217415354521833	1.1579713411000549E-4
32-33	0.0	8.907471854615807E-5	0.0	0.01549009355517689	1.247046059646213E-4
34-35	0.0	9.798219040077388E-5	0.0	0.028797856505972906	1.4251954967385292E-4
36-37	8.907471854615808E-6	1.0688966225538969E-4	0.0	0.052206692539903246	1.4251954967385292E-4
38-39	1.7814943709231616E-5	1.336120778192371E-4	0.0	0.0875604483308734	1.4251954967385292E-4
40-41	1.7814943709231616E-5	1.6033449338308454E-4	0.0	0.126334673314016	1.4251954967385292E-4
42-43	1.7814943709231616E-5	1.6033449338308454E-4	0.0	0.1631937918484162	1.4251954967385292E-4
44-45	3.562988741846323E-5	1.6033449338308454E-4	0.0	0.21084876627061078	1.4251954967385292E-4
46-47	5.3444831127694843E-5	1.6033449338308454E-4	0.0	0.26964698798292974	1.8705690894693194E-4
48-49	5.3444831127694843E-5	1.6033449338308454E-4	0.0	0.33633722975843827	1.9596438080154776E-4
50-51	5.3444831127694843E-5	1.6033449338308454E-4	0.0	0.4051118199479269	1.9596438080154776E-4
52-53	5.3444831127694843E-5	1.6924196523770036E-4	0.0	0.4741625417649087	2.0487185265616358E-4
54-55	5.3444831127694843E-5	1.7814943709231615E-4	0.0	0.5554788523256964	2.1377932451077937E-4
56-57	5.3444831127694843E-5	1.7814943709231615E-4	0.0	0.6513499718969262	2.2268679636539517E-4
58-59	5.3444831127694843E-5	2.1377932451077937E-4	0.0	0.7614285090762685	2.405017400746268E-4
60-61	5.3444831127694843E-5	2.1377932451077937E-4	0.0	0.8799513295737864	2.494092119292426E-4
62-63	5.3444831127694843E-5	2.1377932451077937E-4	0.0	1.0003981639919013	2.8503909934770585E-4
64-65	5.3444831127694843E-5	2.2268679636539517E-4	0.0	1.126599225228098	2.8503909934770585E-4
66-67	5.3444831127694843E-5	2.3159426822001099E-4	0.0	1.273848642456752	2.8503909934770585E-4
68-69	5.3444831127694843E-5	2.3159426822001099E-4	0.0	1.446324019977678	2.8503909934770585E-4
70-71	5.3444831127694843E-5	2.3159426822001099E-4	0.0	1.6351357008799692	2.9394657120232167E-4
72-73	8.907471854615807E-5	2.3159426822001099E-4	0.0	1.827759779736036	3.562988741846323E-4
74-75	8.907471854615807E-5	2.3159426822001099E-4	0.0	2.0243744059829707	3.562988741846323E-4
76-77	8.907471854615807E-5	2.3159426822001099E-4	0.0	2.239863965089836	3.562988741846323E-4
78-79	8.907471854615807E-5	2.3159426822001099E-4	0.0	2.4877945366912124	3.562988741846323E-4
80-81	8.907471854615807E-5	2.405017400746268E-4	0.0	2.7615924065583934	3.741138178938639E-4
82-83	8.907471854615807E-5	2.494092119292426E-4	0.0	3.0479943490998553	4.0974370531232716E-4
84-85	8.907471854615807E-5	2.672241556384742E-4	0.0	3.332730594404504	4.0974370531232716E-4
86-87	8.907471854615807E-5	2.672241556384742E-4	0.0	3.6355757299895872	4.0974370531232716E-4
88	1.4251954967385292E-4	2.672241556384742E-4	0.0	3.878295430556013	4.0974370531232716E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10490	0.0	47.523853	1
GTATCAA	16845	0.0	35.262543	1
TACCTGG	5150	0.0	29.587444	2
GTACCTG	5550	0.0	28.218409	1
TCAACGC	21055	0.0	27.608053	4
ATCAACG	21370	0.0	27.223577	3
CAACGCA	21570	0.0	27.057579	5
TATCAAC	22465	0.0	26.14723	2
AACGCAG	22485	0.0	26.060764	6
GTACATG	19680	0.0	25.116488	1
ACCTGGG	6090	0.0	24.075005	3
TACATGG	20425	0.0	23.647106	2
ACATGGG	20015	0.0	23.431713	3
ACGCAGA	25425	0.0	22.95484	7
CGCAGAG	26095	0.0	22.329447	8
CATGGGG	11730	0.0	21.112223	4
GAGTACT	17225	0.0	19.670385	12-13
GCAGAGT	29000	0.0	19.622746	9
AGAGTAC	26410	0.0	19.296677	10-11
AGTACTT	18600	0.0	18.405745	12-13
>>END_MODULE