FastQCFastQC Report
Mon 6 Jun 2016
ERR1211543_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211543_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5747633
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222200.38659392483827687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191370.3329544527286276No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122570.21325300345376957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101050.050.4589231
GTATCAA177950.038.363291
TACCTGG50250.032.001062
TCAACGC215350.030.8323754
ATCAACG217300.030.5775833
CAACGCA217550.030.4960585
AACGCAG225350.029.5792456
TATCAAC230750.028.8125722
GTACCTG58550.028.6265451
GTACATG204950.028.3703611
TACATGG210450.026.9670032
ACGCAGA255800.025.8031127
ACATGGG211400.025.6738193
CGCAGAG260750.025.1344188
ACCTGGG63500.024.7164553
CATGGGG119200.023.9682884
GCAGAGT287100.022.3380329
GAGTACT182600.021.0488412-13
ATGGGGG70450.020.6084635
CAGAGTA281850.019.76191110-11
Produced by FastQC (version 0.11.5)