FastQCFastQC Report
Mon 6 Jun 2016
ERR1211543_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211543_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5747633
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231620.40298328024771246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208790.36326258130955824No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146720.2552702999652205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119150.048.5030941
GTATCAA199000.035.0901261
TACCTGG53750.034.8186872
GTACCTG62250.030.7444421
TCAACGC243200.028.2684064
ATCAACG244600.028.1452753
CAACGCA247650.027.7604545
TATCAAC254150.027.216382
AACGCAG256650.026.8782846
ACCTGGG66800.026.8726963
GTACATG219850.025.9659251
TACATGG223500.024.8473552
ACATGGG223200.023.9592063
ACGCAGA288750.023.7761347
CGCAGAG294750.023.228378
CATGGGG115500.022.2955994
GCAGAGT321250.020.9754589
GAGTACT194250.020.9130312-13
ATGGGGG67400.019.6611885
AGAGTAC296350.019.28153810-11
Produced by FastQC (version 0.11.5)