##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211543_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5747633 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12957873267135 33.0 31.0 34.0 30.0 34.0 2 32.341472045970924 34.0 31.0 34.0 30.0 34.0 3 32.350596323738834 34.0 31.0 34.0 30.0 34.0 4 35.99085153836371 37.0 35.0 37.0 35.0 37.0 5 35.91515811813315 37.0 35.0 37.0 35.0 37.0 6 35.934479637095826 37.0 35.0 37.0 35.0 37.0 7 35.86086376774578 37.0 35.0 37.0 35.0 37.0 8 35.85434699814689 37.0 35.0 37.0 35.0 37.0 9 37.50190678493216 39.0 37.0 39.0 35.0 39.0 10-11 37.46517148885463 39.0 37.0 39.0 34.5 39.0 12-13 37.38280305997269 39.0 37.0 39.0 34.0 39.0 14-15 38.61704583434607 40.0 38.0 41.0 34.0 41.0 16-17 38.547566102428604 40.0 38.0 41.0 34.0 41.0 18-19 38.53785088574723 40.0 38.0 41.0 34.0 41.0 20-21 38.47992225669245 40.0 38.0 41.0 34.0 41.0 22-23 38.37064901325468 40.0 38.0 41.0 34.0 41.0 24-25 38.259222274630275 40.0 38.0 41.0 33.5 41.0 26-27 38.085158272979506 40.0 37.0 41.0 33.0 41.0 28-29 38.120426269387764 40.0 38.0 41.0 33.0 41.0 30-31 38.03843617015909 40.0 37.0 41.0 33.0 41.0 32-33 37.846816506899444 40.0 37.0 41.0 33.0 41.0 34-35 37.72836826916402 40.0 37.0 41.0 33.0 41.0 36-37 37.6644518708832 40.0 37.0 41.0 33.0 41.0 38-39 37.565315669946216 40.0 37.0 41.0 32.0 41.0 40-41 37.399569005188745 40.0 36.0 41.0 31.5 41.0 42-43 37.24611200819537 40.0 36.0 41.0 31.0 41.0 44-45 37.059694225431585 39.5 36.0 41.0 31.0 41.0 46-47 36.793167552625576 39.0 35.5 41.0 30.0 41.0 48-49 36.562455884013474 39.0 35.0 41.0 30.0 41.0 50-51 36.361784146621744 39.0 35.0 41.0 29.0 41.0 52-53 36.16527690616294 39.0 35.0 40.0 28.5 41.0 54-55 35.966275073582466 39.0 35.0 40.0 28.0 41.0 56-57 35.76855472504943 38.0 34.5 40.0 28.0 41.0 58-59 35.50788507199398 38.0 34.0 40.0 27.0 41.0 60-61 35.198509195002536 38.0 34.0 40.0 26.0 41.0 62-63 34.87559931192545 37.0 34.0 40.0 26.0 41.0 64-65 34.56377764551077 37.0 33.0 40.0 26.0 41.0 66-67 34.22016532718773 36.0 33.0 39.0 25.5 41.0 68-69 33.82062598638431 36.0 32.5 39.0 25.0 40.0 70-71 33.43605880890446 35.0 32.0 38.5 24.0 40.0 72-73 33.03221125983514 35.0 31.5 38.0 23.5 40.0 74-75 32.622932344497286 35.0 31.0 37.0 22.5 39.0 76-77 31.07806831090294 33.5 29.5 35.5 21.0 38.5 78-79 31.91239228391931 34.0 31.0 36.0 22.0 39.0 80-81 31.87804518834101 34.0 31.0 36.0 22.0 37.5 82-83 31.595701656664577 34.0 31.0 35.5 21.5 37.0 84-85 31.296111634128344 34.0 30.5 35.0 20.0 37.0 86-87 31.00543528092347 34.0 30.0 35.0 20.0 36.0 88-89 30.724064062545402 34.0 30.0 35.0 19.5 36.0 90-91 30.43613397027959 34.0 29.0 35.0 19.0 35.5 92-93 30.221845149124867 34.0 29.0 35.0 18.5 35.0 94-95 29.999807659953238 34.0 29.0 35.0 18.0 35.0 96-97 29.788672223852842 34.0 29.0 35.0 18.0 35.0 98-99 29.62417033237856 34.0 29.0 35.0 17.0 35.0 100 27.767742825611865 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3044987918534616 1101 2 0.24014169403366736 1101 3 0.3058727655345308 1101 4 0.169239743680059 1101 5 0.16285660790650525 1101 6 0.18566552138191383 1101 7 0.22913177158128661 1101 8 0.22294683611946198 1101 9 0.2268368118338273 1101 10-11 0.19609014220506538 1101 12-13 0.2073525601650701 1101 14-15 0.29654998239724506 1101 16-17 0.3081123919510276 1101 18-19 0.3292165152760944 1101 20-21 0.24738293661831534 1101 22-23 0.34378524340572625 1101 24-25 0.28837121089779316 1101 26-27 0.20035491386978066 1101 28-29 0.3271722353233244 1101 30-31 0.3353556877827941 1101 32-33 0.41789896420454653 1101 34-35 0.36502502978508744 1101 36-37 0.4201854331760515 1101 38-39 0.3931006102190082 1101 40-41 0.3981610011202079 1101 42-43 0.3417735567278939 1101 44-45 0.35314677578198683 1101 46-47 0.418006743840003 1101 48-49 0.43455413103356477 1101 50-51 0.3035708255830869 1101 52-53 0.30701058020364513 1101 54-55 0.2745302690578839 1101 56-57 0.17098274889127651 1101 58-59 0.25522273412502017 1101 60-61 0.2581163985832333 1101 62-63 0.32096284145696075 1101 64-65 0.2637608183379143 1101 66-67 0.17788600855185166 1101 68-69 0.23166972315002 1101 70-71 0.2818808854891728 1101 72-73 0.10692528384656441 1101 74-75 0.08986185562054061 1101 76-77 0.17090335655445088 1101 78-79 0.24062315352518127 1101 80-81 0.19326516843268138 1101 82-83 0.1688658049216798 1101 84-85 0.166734888205891 1101 86-87 0.2583247293640163 1101 88-89 0.17421338263047303 1101 90-91 0.0194507120248133 1101 92-93 0.02965130650180825 1101 94-95 0.11012666626029599 1101 96-97 0.07787083233123937 1101 98-99 -0.03434215105787075 1101 100 -0.05316014794455981 1102 1 0.2707699019553118 1102 2 0.2420243685359793 1102 3 0.2922533703998198 1102 4 0.18776162666577534 1102 5 0.18402765385241082 1102 6 0.18481214404260982 1102 7 0.16437850362742523 1102 8 0.18361126202635347 1102 9 0.25054317143763427 1102 10-11 0.2199973507888302 1102 12-13 0.24699078037055244 1102 14-15 0.34100580821549187 1102 16-17 0.35188381702199933 1102 18-19 0.3000766952589373 1102 20-21 0.29154734367752866 1102 22-23 0.3092766802959446 1102 24-25 0.3199473542787814 1102 26-27 0.30704283897191686 1102 28-29 0.3455398776182719 1102 30-31 0.34389569038724943 1102 32-33 0.4239864056391909 1102 34-35 0.3805959285147793 1102 36-37 0.42566068839706617 1102 38-39 0.3191742991306583 1102 40-41 0.4233816130735022 1102 42-43 0.35626054805399576 1102 44-45 0.4130353250954144 1102 46-47 0.459241821154734 1102 48-49 0.43769370281788156 1102 50-51 0.40563261330323286 1102 52-53 0.49778757780894267 1102 54-55 0.4847905287106187 1102 56-57 0.46850276195637264 1102 58-59 0.5455531046599376 1102 60-61 0.5653191667758435 1102 62-63 0.5847021242895778 1102 64-65 0.5548409717183276 1102 66-67 0.49422696830294655 1102 68-69 0.48689699789736096 1102 70-71 0.5036266045005746 1102 72-73 0.4840172432322092 1102 74-75 0.4167340481820574 1102 76-77 0.47850661625891533 1102 78-79 0.5136003691627273 1102 80-81 0.4504953685457238 1102 82-83 0.44430621816903937 1102 84-85 0.373456118660787 1102 86-87 0.28741434636920005 1102 88-89 0.25476549413247085 1102 90-91 0.188007349332036 1102 92-93 0.1901331131050874 1102 94-95 0.2743127115809365 1102 96-97 0.3003651769828686 1102 98-99 0.1995721969900366 1102 100 0.26926893032937116 1103 1 0.2597183751542502 1103 2 0.22769300823163974 1103 3 0.2750557959881661 1103 4 0.16488677651195616 1103 5 0.1439264215375431 1103 6 0.16347844811316037 1103 7 0.16273419206405748 1103 8 0.1846535085304737 1103 9 0.1862594151543817 1103 10-11 0.18426161535765573 1103 12-13 0.23231309924321408 1103 14-15 0.32578422084234404 1103 16-17 0.3218053995753465 1103 18-19 0.30123510243235785 1103 20-21 0.22783444204606695 1103 22-23 0.24167539654730064 1103 24-25 0.27758906015344564 1103 26-27 0.2643090661380896 1103 28-29 0.31045164042361506 1103 30-31 0.3670688806448297 1103 32-33 0.3682991184193085 1103 34-35 0.3344148605330304 1103 36-37 0.318339977639134 1103 38-39 0.26947419432165276 1103 40-41 0.2639674482379277 1103 42-43 0.3707587783937143 1103 44-45 0.3671978130688345 1103 46-47 0.3709272584969412 1103 48-49 0.3042067297642532 1103 50-51 0.36221374486003555 1103 52-53 0.3666777149531484 1103 54-55 0.40020128133709676 1103 56-57 0.48685896086728775 1103 58-59 0.4995898937737877 1103 60-61 0.42266260015923507 1103 62-63 0.45767739589135914 1103 64-65 0.4424363330959693 1103 66-67 0.5015134574829645 1103 68-69 0.5400152278951467 1103 70-71 0.38086800677584876 1103 72-73 0.397522700091244 1103 74-75 0.427074508934119 1103 76-77 0.3331562473796694 1103 78-79 0.3851113353770579 1103 80-81 0.4207686447001464 1103 82-83 0.3907709640035577 1103 84-85 0.33194259427967765 1103 86-87 0.2974348310213131 1103 88-89 0.3436493539401013 1103 90-91 0.35648537870232033 1103 92-93 0.3280914448809149 1103 94-95 0.3638151532127658 1103 96-97 0.39243655510020403 1103 98-99 0.3576846161774121 1103 100 0.45921259657017544 1104 1 0.2436265005332814 1104 2 0.23557685050098343 1104 3 0.3033358845847758 1104 4 0.0609547568089539 1104 5 0.11469766186576891 1104 6 0.12546783358450142 1104 7 0.12040810459114937 1104 8 0.11451593559073814 1104 9 0.1885641339561488 1104 10-11 0.2026984689468705 1104 12-13 0.22452766532781254 1104 14-15 0.27560840680407495 1104 16-17 0.3056390763683865 1104 18-19 0.2295128035356413 1104 20-21 0.1662303398235423 1104 22-23 0.22854214727311728 1104 24-25 0.17178203408784043 1104 26-27 0.28673378495924595 1104 28-29 0.31975017549650175 1104 30-31 0.26092977486166546 1104 32-33 0.21540773070154984 1104 34-35 0.4067517389909554 1104 36-37 0.32586180563005485 1104 38-39 0.3515704667780213 1104 40-41 0.4232778723003676 1104 42-43 0.46523342806239043 1104 44-45 0.3870525104680027 1104 46-47 0.34010925238592193 1104 48-49 0.3845891698357562 1104 50-51 0.428424933550545 1104 52-53 0.31716534531391716 1104 54-55 0.39124935696900565 1104 56-57 0.4132518026533205 1104 58-59 0.39385099004284996 1104 60-61 0.4131680967263378 1104 62-63 0.46928811522734293 1104 64-65 0.5500235565890961 1104 66-67 0.5011843591022469 1104 68-69 0.49436133831707707 1104 70-71 0.36197279082768574 1104 72-73 0.4540583115537231 1104 74-75 0.43413916225312477 1104 76-77 0.37472692954347053 1104 78-79 0.4343569602046635 1104 80-81 0.5192492051720734 1104 82-83 0.3982073633630776 1104 84-85 0.4707986601851566 1104 86-87 0.5081968510200241 1104 88-89 0.4596041113053353 1104 90-91 0.3469530268347647 1104 92-93 0.4186027708098692 1104 94-95 0.4900097845765252 1104 96-97 0.5023725751270298 1104 98-99 0.5111905592178836 1104 100 0.5855849204567924 1105 1 0.28218456960519234 1105 2 0.1891109753687843 1105 3 0.27163093952962214 1105 4 0.17037220800907704 1105 5 0.04334113488167901 1105 6 0.14240346492219658 1105 7 0.16575094841986981 1105 8 0.17294310691909232 1105 9 0.17223159761250173 1105 10-11 0.16917994668649072 1105 12-13 0.1914606548147617 1105 14-15 0.23922818913313648 1105 16-17 0.254989137156052 1105 18-19 0.28898582145416896 1105 20-21 0.23066009727904913 1105 22-23 0.2722394381147808 1105 24-25 0.3648909452324176 1105 26-27 0.33571537973096355 1105 28-29 0.3626725192661695 1105 30-31 0.44222129223657447 1105 32-33 0.47147588618394565 1105 34-35 0.5025400139176313 1105 36-37 0.4883407476949557 1105 38-39 0.5073990358901526 1105 40-41 0.49459499115440764 1105 42-43 0.5062943903060173 1105 44-45 0.5250395883664467 1105 46-47 0.5713967222697605 1105 48-49 0.6252188013107514 1105 50-51 0.6126962828736211 1105 52-53 0.5561237583158913 1105 54-55 0.5185814303298812 1105 56-57 0.502983386851362 1105 58-59 0.5503029104298918 1105 60-61 0.6189746838831311 1105 62-63 0.5242979992632684 1105 64-65 0.5873713191548617 1105 66-67 0.5910696601081966 1105 68-69 0.5611176814885965 1105 70-71 0.6035089724484664 1105 72-73 0.5637761681681326 1105 74-75 0.5412823878386845 1105 76-77 0.5038682502354135 1105 78-79 0.6372903181701624 1105 80-81 0.6437569831948622 1105 82-83 0.6169785977876074 1105 84-85 0.5750918370941491 1105 86-87 0.6518465383466285 1105 88-89 0.6826742205022533 1105 90-91 0.6824906258931911 1105 92-93 0.6833850553242691 1105 94-95 0.6971342599244181 1105 96-97 0.7679920690488267 1105 98-99 0.8114109811885939 1105 100 0.9101785178404924 1106 1 0.25403855855038415 1106 2 0.16651543418813475 1106 3 0.2125188553466728 1106 4 0.1737704678178673 1106 5 0.20046586831057311 1106 6 0.19023837133739363 1106 7 0.20402238562429886 1106 8 0.17780222638268128 1106 9 0.218444454466578 1106 10-11 0.21294862709181928 1106 12-13 0.24573111645483436 1106 14-15 0.32577125792402484 1106 16-17 0.33283463764588817 1106 18-19 0.3123671126756591 1106 20-21 0.3025424847796856 1106 22-23 0.27390826878301056 1106 24-25 0.318976766778448 1106 26-27 0.35597130727914816 1106 28-29 0.3734650234483041 1106 30-31 0.4553099405439909 1106 32-33 0.46799209574238887 1106 34-35 0.3755912642381958 1106 36-37 0.4442460586739898 1106 38-39 0.42724594658866266 1106 40-41 0.48476927114280244 1106 42-43 0.46607207816166607 1106 44-45 0.47557023609459037 1106 46-47 0.5582572580323273 1106 48-49 0.5602008910110001 1106 50-51 0.5648097738648232 1106 52-53 0.6386763999398255 1106 54-55 0.6699424826766531 1106 56-57 0.6295508203898379 1106 58-59 0.6656796738187865 1106 60-61 0.68416418834952 1106 62-63 0.6743231699603882 1106 64-65 0.7889712837702234 1106 66-67 0.7493602162829021 1106 68-69 0.7480927580068553 1106 70-71 0.7488340185431355 1106 72-73 0.7201658406049631 1106 74-75 0.6706891567083488 1106 76-77 0.7199634810899624 1106 78-79 0.7313690658342189 1106 80-81 0.8317065836382795 1106 82-83 0.7591486280670523 1106 84-85 0.7115082860368673 1106 86-87 0.6103493871092383 1106 88-89 0.7160431702408978 1106 90-91 0.7628680158215708 1106 92-93 0.799665504851248 1106 94-95 0.803065134932357 1106 96-97 0.8281743455994466 1106 98-99 0.8649272608362288 1106 100 0.9524902084683404 1107 1 0.17168594027862127 1107 2 0.17260593745233166 1107 3 0.22792595607643307 1107 4 0.14196799738016352 1107 5 0.09472487965155807 1107 6 0.11270647605309847 1107 7 0.17946174368464085 1107 8 0.15117132976829595 1107 9 0.18683122318274314 1107 10-11 0.16118910021859278 1107 12-13 0.15702499222076938 1107 14-15 0.24221856005625142 1107 16-17 0.22466306346142773 1107 18-19 0.14882873053318235 1107 20-21 0.21337193010311495 1107 22-23 0.3131926378094718 1107 24-25 0.28522484414114047 1107 26-27 0.34026012378686943 1107 28-29 0.34198983923807447 1107 30-31 0.24955849678287478 1107 32-33 0.3197642987497815 1107 34-35 0.32449542869135684 1107 36-37 0.43622063923299237 1107 38-39 0.41003466360137253 1107 40-41 0.4435307550522296 1107 42-43 0.4323564504384123 1107 44-45 0.4560897084062603 1107 46-47 0.3833908901275791 1107 48-49 0.4134814095262911 1107 50-51 0.40637028543171283 1107 52-53 0.476821758723176 1107 54-55 0.44070994495432103 1107 56-57 0.4998834479285392 1107 58-59 0.5399980607500225 1107 60-61 0.5239224136618503 1107 62-63 0.44721848928881514 1107 64-65 0.5278538446237988 1107 66-67 0.5607329296581796 1107 68-69 0.46983310455286187 1107 70-71 0.5709032584048543 1107 72-73 0.48231145507785556 1107 74-75 0.5778957014939223 1107 76-77 0.5386099841562455 1107 78-79 0.6998370918206582 1107 80-81 0.6515878360692255 1107 82-83 0.6125866954008004 1107 84-85 0.5497014060379648 1107 86-87 0.5453290348716102 1107 88-89 0.45200399051317675 1107 90-91 0.3611003812784901 1107 92-93 0.5483707153924477 1107 94-95 0.682169304224832 1107 96-97 0.7132110179083817 1107 98-99 0.7391035378303492 1107 100 0.8952883404900973 1108 1 0.17924207607687492 1108 2 0.16423931973920958 1108 3 0.2320700029229812 1108 4 0.12240393215899559 1108 5 0.08529665865262359 1108 6 0.0976415261927599 1108 7 0.13399459975433814 1108 8 0.13351871541989624 1108 9 0.1089619587307169 1108 10-11 0.2082039188932825 1108 12-13 0.2072760760142245 1108 14-15 0.21944689227941439 1108 16-17 0.23359623327475276 1108 18-19 0.23990481515139805 1108 20-21 0.20086539906859713 1108 22-23 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-0.2144246153417697 2316 78-79 -0.04140449398599344 2316 80-81 -0.019384603508207476 2316 82-83 0.01901230718489444 2316 84-85 -0.0804750327135757 2316 86-87 -0.08573552720828914 2316 88-89 -0.014811004257627047 2316 90-91 0.061411844051757924 2316 92-93 0.11681093472730453 2316 94-95 0.1813875695428031 2316 96-97 0.24217373050067081 2316 98-99 0.08518312568311259 2316 100 0.06286571868152535 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 14.0 11 27.0 12 78.0 13 202.0 14 626.0 15 1539.0 16 3615.0 17 7495.0 18 13626.0 19 20855.0 20 28917.0 21 38723.0 22 49576.0 23 55713.0 24 59046.0 25 64389.0 26 73735.0 27 88236.0 28 106638.0 29 128764.0 30 154031.0 31 187657.0 32 227884.0 33 285918.0 34 374757.0 35 512746.0 36 744904.0 37 1161058.0 38 1165153.0 39 191689.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.26956230796895 20.200217731606905 13.710670988820596 23.819548971603545 2 15.00260291073322 21.443092014200225 39.89563981983331 23.658665255233245 3 17.029677165222814 27.29546884395252 31.048263061488097 24.62659092933657 4 11.85741255963573 17.633559589971682 38.304437519432014 32.20459033096058 5 12.217825568550651 38.43538284901418 35.6733726509351 13.673418931500066 6 30.27945785305257 38.21401410990653 18.109476436483934 13.397051600556964 7 26.39229053072804 33.22162357965444 23.112157648200572 17.273928241416943 8 25.667223944395413 36.167411588488584 20.54367896905534 17.621685498060664 9 26.481845045055074 15.051955309595325 20.648573186313946 37.81762645903565 10-11 24.283396312882193 26.565640151345782 29.313362561597096 19.837600974174936 12-13 24.8948828152389 24.51369981347104 29.213999919619084 21.377417451670976 14-15 22.34307583660961 25.804753017459536 26.777718758313206 25.07445238761765 16-17 21.705571547292067 28.642944303665548 27.54588382445397 22.10560032458842 18-19 21.583599988969272 27.539539891691835 29.695855552461502 21.18100456687739 20-21 22.30305807819625 26.915973772366335 29.644288305621476 21.13667984381594 22-23 22.10014974860086 26.849243853948224 29.384957251097976 21.66564914635294 24-25 21.83075569208328 26.9778928087254 29.536250755093572 21.655100744097744 26-27 21.83302448091132 27.050243918309853 29.446922474533377 21.66980912624545 28-29 21.804716856797494 27.01278060191446 29.66279271571371 21.51970982557434 30-31 22.074107801834035 26.99956642365281 29.540468227657506 21.38585754685565 32-33 21.869267028751505 27.16033841755379 29.412352178076702 21.55804237561801 34-35 22.061523345661527 27.230890931379264 29.41215721103585 21.295428511923355 36-37 22.105588329809812 27.126965201101616 29.240041916866737 21.527404552221835 38-39 22.013995516354345 27.280826368777678 29.25209067159862 21.453087443269357 40-41 22.052480158126013 27.130712341328085 29.359231223623556 21.457576276922357 42-43 21.925753424490686 27.233230063500898 29.11222714410035 21.728789367908067 44-45 21.87329485505572 27.45633958970733 29.008369477766298 21.66199607747065 46-47 21.93666273055835 27.392756128118716 28.759139315527786 21.911441825795148 48-49 21.920817937981273 27.493820834206957 28.723555301902596 21.86180592590917 50-51 21.936110436622386 27.67825939511078 28.529950880884208 21.855679287382628 52-53 22.03056827822548 27.676490361896068 28.39980923534762 21.89313212453083 54-55 21.929115709308764 27.677108290770576 28.509558123032242 21.88421787688842 56-57 22.035765885896584 27.755534243829654 28.22922814279908 21.97947172747468 58-59 21.914003193766412 27.640077001158424 28.50533958909749 21.940580215977672 60-61 22.10932581849337 27.827800151934696 28.363025442275774 21.69984858729616 62-63 21.953379555877277 27.76082835160571 28.4883244429325 21.797467649584508 64-65 21.846806072539227 27.576025706863494 28.483555518746734 22.093612701850546 66-67 21.85917179552185 27.60303139397038 28.354711514184906 22.183085296322865 68-69 21.791594686281524 27.722259304784323 28.364636390591443 22.121509618342714 70-71 21.805812704976436 27.54328244515214 28.35311390186743 22.297790948003996 72-73 21.76263065781496 27.853779303424535 28.08738804254198 22.296201996218517 74-75 21.79712228263139 27.886686529312364 27.995369094447085 22.320822093609156 76-77 21.71635211885883 27.768515710513363 28.18714433183232 22.327987838795487 78-79 21.689003193815843 27.683312945146078 28.144146006390415 22.48353785464767 80-81 21.684204885228393 27.948331613831908 27.852904287061126 22.514559213878574 82-83 21.81856955035178 27.89246913469609 27.886065463443522 22.40289585150861 84-85 21.730382766439675 27.90290195574811 27.83394843355434 22.532766844257875 86-87 21.579491234658608 27.901556144370492 28.0070573101475 22.5118953108234 88-89 21.574343871118636 27.75490078017107 27.971208917115874 22.699546431594424 90-91 21.782179337785593 27.806747163738173 27.897153227052694 22.51392027142354 92-93 21.67348205739722 27.795994793668616 27.786213560009653 22.744309588924512 94-95 21.48680376073913 27.67781050956315 27.84903958641164 22.986346143286084 96-97 21.57293658695159 27.624700657340036 27.873293631547448 22.92906912416092 98-99 21.50394848600911 27.55211616959009 27.801853711555967 23.14208163284483 100 20.709751606253572 28.047148558400252 27.931950046305627 23.31114978904055 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3250.0 1 2366.0 2 2608.5 3 3632.5 4 2682.0 5 2124.5 6 3180.5 7 5977.0 8 9307.5 9 10690.0 10 8879.0 11 6766.0 12 6658.5 13 7107.0 14 7522.0 15 7893.5 16 8428.0 17 9018.0 18 9679.0 19 10399.0 20 11425.5 21 13015.5 22 15330.0 23 18777.5 24 23189.5 25 28570.0 26 35479.5 27 43406.5 28 51843.0 29 61512.0 30 73076.5 31 84222.0 32 96309.0 33 109749.0 34 123357.5 35 136243.5 36 153206.5 37 169107.0 38 177915.0 39 185434.5 40 188121.5 41 187916.0 42 189902.0 43 193784.0 44 198031.0 45 203942.5 46 207398.0 47 207394.0 48 209359.5 49 212145.5 50 211949.5 51 204455.5 52 194157.0 53 185500.5 54 175874.0 55 163153.0 56 148018.0 57 132373.5 58 116803.5 59 100512.0 60 84006.0 61 69150.0 62 55775.0 63 43325.0 64 32194.5 65 23766.0 66 17383.5 67 12890.5 68 9783.5 69 7382.5 70 5795.0 71 4468.5 72 3367.5 73 2631.0 74 2064.0 75 1537.0 76 1119.0 77 782.0 78 507.5 79 333.0 80 234.5 81 162.0 82 114.5 83 93.5 84 74.0 85 51.0 86 37.5 87 23.0 88 21.0 89 16.0 90 9.5 91 5.5 92 3.0 93 3.5 94 4.0 95 2.0 96 0.5 97 2.0 98 3.5 99 3.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0033057086282300904 2 0.07068996924473084 3 8.525248567540759E-4 4 5.219539939310669E-4 5 0.0013918773171495119 6 6.959386585747559E-4 7 0.0 8 8.699233232184448E-4 9 1.73984664643689E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 8.090286905931537E-4 18-19 8.960210229149981E-4 20-21 0.003192618596211693 22-23 0.0 24-25 1.73984664643689E-5 26-27 2.0878159757242677E-4 28-29 9.656148887724739E-4 30-31 0.001513666582400094 32-33 0.003079528564193295 34-35 0.004132135785287613 36-37 0.0011482987866483472 38-39 0.001357080384220774 40-41 0.00553271233566931 42-43 0.006811499620800424 44-45 0.005584907735062416 46-47 0.007246461282409646 48-49 0.008664436299255712 50-51 0.008377361602593624 52-53 0.004123436552055429 54-55 0.002427086071779461 56-57 0.00252277763733349 58-59 0.003253513228836984 60-61 0.007429145180285519 62-63 0.01388397623856638 64-65 0.003801564922464604 66-67 0.009986719750547747 68-69 0.012126731125665122 70-71 0.00567190006738426 72-73 0.007246461282409646 74-75 0.0028011531007633922 76-77 0.003062130097728926 78-79 0.007524836745839548 80-81 0.005697997767080814 82-83 0.016171874578630892 84-85 0.017546353429316035 86-87 0.02176548154692549 88-89 0.020895558223707048 90-91 0.023261749662861216 92-93 0.03369213030825037 94-95 0.036771658872443665 96-97 0.022478818671964617 98-99 0.0173984664643689 100 0.017937818924764332 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5747633.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.25558390284833 #Duplication Level Percentage of deduplicated Percentage of total 1 75.29942657580094 45.3721091587454 2 15.355915649529273 18.505593276505454 3 4.625101389171718 8.360645544432503 4 1.8357226521525418 4.42450161156547 5 0.8920925543403452 2.6876778878580474 6 0.5205008182101744 1.881784843389862 7 0.32101041716662193 1.3539869087690213 8 0.2178531621647972 1.0501495593057408 9 0.16020241334052618 0.8687780962630975 >10 0.6992053122483923 7.3159838879906625 >50 0.04271783905310145 1.7822391061572553 >100 0.02706851220063478 3.130112891389092 >500 0.002318266546959744 0.9834543355993115 >1k 7.465601548593771E-4 0.8928933462665144 >5k 0.0 0.0 >10k+ 1.1787791918829754E-4 1.390089545762469 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23162 0.40298328024771246 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20879 0.36326258130955824 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14672 0.2552702999652205 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.479693292873779E-5 2 0.0 0.0 0.0 0.0 3.479693292873779E-5 3 0.0 1.7398466464368896E-5 0.0 0.0 3.479693292873779E-5 4 0.0 1.7398466464368896E-5 0.0 0.0 3.479693292873779E-5 5 0.0 1.7398466464368896E-5 0.0 0.0 3.479693292873779E-5 6 0.0 1.7398466464368896E-5 0.0 0.0 5.219539939310669E-5 7 0.0 1.7398466464368896E-5 0.0 0.0 5.219539939310669E-5 8 0.0 1.7398466464368896E-5 0.0 0.0 5.219539939310669E-5 9 0.0 1.7398466464368896E-5 0.0 0.0 8.699233232184449E-5 10-11 0.0 1.7398466464368896E-5 0.0 0.0 8.699233232184449E-5 12-13 0.0 1.7398466464368896E-5 0.0 0.0 9.569156555402894E-5 14-15 0.0 2.6097699696553346E-5 0.0 0.0 1.0439079878621338E-4 16-17 1.7398466464368896E-5 3.479693292873779E-5 0.0 1.7398466464368896E-5 1.0439079878621338E-4 18-19 1.7398466464368896E-5 3.479693292873779E-5 0.0 2.6097699696553346E-5 1.0439079878621338E-4 20-21 1.7398466464368896E-5 3.479693292873779E-5 0.0 4.349616616092224E-5 1.1309003201839782E-4 22-23 3.479693292873779E-5 3.479693292873779E-5 0.0 2.0008236434024233E-4 1.5658619817932008E-4 24-25 6.959386585747558E-5 3.479693292873779E-5 0.0 4.0886396191266907E-4 1.9138313110805786E-4 26-27 6.959386585747558E-5 3.479693292873779E-5 0.0 6.959386585747558E-4 1.9138313110805786E-4 28-29 6.959386585747558E-5 3.479693292873779E-5 0.0 0.0019312297775449477 2.000823643402423E-4 30-31 6.959386585747558E-5 3.479693292873779E-5 0.0 0.006489627991209598 2.0878159757242677E-4 32-33 6.959386585747558E-5 3.479693292873779E-5 0.0 0.016041386080148122 2.2618006403679567E-4 34-35 6.959386585747558E-5 3.479693292873779E-5 0.0 0.030003655417804163 2.2618006403679567E-4 36-37 1.0439079878621338E-4 5.219539939310669E-5 0.0 0.05315231504864698 2.2618006403679567E-4 38-39 1.0439079878621338E-4 6.959386585747558E-5 0.0 0.0907851980110769 2.2618006403679567E-4 40-41 1.2178926525058228E-4 6.959386585747558E-5 0.0 0.1331330653853508 2.4357853050116455E-4 42-43 1.3918773171495117E-4 6.959386585747558E-5 0.0 0.1747588964013534 2.4357853050116455E-4 44-45 1.3918773171495117E-4 6.959386585747558E-5 0.0 0.22662372493163707 2.4357853050116455E-4 46-47 1.3918773171495117E-4 6.959386585747558E-5 0.0 0.2915722002431262 2.4357853050116455E-4 48-49 1.3918773171495117E-4 6.959386585747558E-5 0.0 0.3628711158141099 2.6097699696553346E-4 50-51 1.3918773171495117E-4 6.959386585747558E-5 0.0 0.4387893242313836 2.6097699696553346E-4 52-53 1.3918773171495117E-4 6.959386585747558E-5 0.0 0.5159515230008597 2.783754634299024E-4 54-55 1.6528543141150452E-4 1.0439079878621338E-4 0.0 0.6021957212647363 2.957739298942713E-4 56-57 1.9138313110805786E-4 1.1309003201839782E-4 0.0 0.7062472499548944 3.044731631264557E-4 58-59 2.000823643402423E-4 1.2178926525058228E-4 0.0 0.8240783640848328 3.218716295908246E-4 60-61 2.0878159757242677E-4 1.2178926525058228E-4 0.0 0.9529923013525742 3.3057086282300903E-4 62-63 2.1748083080461124E-4 1.2178926525058228E-4 0.0 1.0849944664177409 3.3057086282300903E-4 64-65 2.2618006403679567E-4 1.2178926525058228E-4 0.0 1.2177186678411793 3.392700960551935E-4 66-67 2.2618006403679567E-4 1.3918773171495117E-4 0.0 1.3744092568192854 3.566685625195624E-4 68-69 2.2618006403679567E-4 1.3918773171495117E-4 0.0 1.5586503174437198 3.6536779575174684E-4 70-71 2.2618006403679567E-4 1.3918773171495117E-4 0.0 1.7601767545005047 3.6536779575174684E-4 72-73 2.2618006403679567E-4 1.3918773171495117E-4 0.0 1.9639997891305865 4.871570610023291E-4 74-75 2.2618006403679567E-4 1.3918773171495117E-4 0.0 2.1670659904694682 4.871570610023291E-4 76-77 2.2618006403679567E-4 1.3918773171495117E-4 0.0 2.3934200391709073 4.871570610023291E-4 78-79 2.2618006403679567E-4 1.3918773171495117E-4 0.0 2.6575983539658847 4.871570610023291E-4 80-81 2.2618006403679567E-4 1.3918773171495117E-4 0.0 2.9447078475609003 5.04555527466698E-4 82-83 2.2618006403679567E-4 1.3918773171495117E-4 0.0 3.241264708446068 5.393524603954359E-4 84-85 2.2618006403679567E-4 1.3918773171495117E-4 0.0 3.535377084792992 5.393524603954359E-4 86-87 2.2618006403679567E-4 1.3918773171495117E-4 0.0 3.845348163322188 5.393524603954359E-4 88 2.2618006403679567E-4 1.3918773171495117E-4 0.0 4.102523595365257 5.741493933241736E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11915 0.0 48.503094 1 GTATCAA 19900 0.0 35.090126 1 TACCTGG 5375 0.0 34.818687 2 GTACCTG 6225 0.0 30.744442 1 TCAACGC 24320 0.0 28.268406 4 ATCAACG 24460 0.0 28.145275 3 CAACGCA 24765 0.0 27.760454 5 TATCAAC 25415 0.0 27.21638 2 AACGCAG 25665 0.0 26.878284 6 ACCTGGG 6680 0.0 26.872696 3 GTACATG 21985 0.0 25.965925 1 TACATGG 22350 0.0 24.847355 2 ACATGGG 22320 0.0 23.959206 3 ACGCAGA 28875 0.0 23.776134 7 CGCAGAG 29475 0.0 23.22837 8 CATGGGG 11550 0.0 22.295599 4 GCAGAGT 32125 0.0 20.975458 9 GAGTACT 19425 0.0 20.91303 12-13 ATGGGGG 6740 0.0 19.661188 5 AGAGTAC 29635 0.0 19.281538 10-11 >>END_MODULE