FastQCFastQC Report
Mon 6 Jun 2016
ERR1211542_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211542_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2754483
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA457561.661146574511442No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229660.833768079164039No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202350.7346206166456646No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG194030.7044153113306562No Hit
GTACCTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA191540.695375502408256No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT163410.5932510747025849No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156110.5667488236449454No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135580.49221578060202226No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG87880.31904353738977514No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG85830.3116011244215339No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC68830.24988355346538715No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63690.23122306436452866No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT52630.19107033878952964No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG41670.1512806577495668No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT38280.13897344801184106No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC37250.13523408930096864No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG36150.13124059941557092No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG36050.13087755488053476No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG32830.11918752085237048No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30810.11185402124464011No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30690.11141836780259672No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC30620.1111642366280714No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA28130.10212442770567108No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG91550.072.364152
TATAACG9100.072.33652
ACCTGGG90350.070.2635043
TAACGCA10250.066.473234
GTACCTG109400.060.9928361
TACCCGG6200.059.152592
CCTGGGG36250.057.943154
GTACCCG6850.057.7450831
ATAACGC12550.054.6653023
ATCACGC4150.054.349533
GTATAAC13050.053.0436171
TATCACG4350.051.8827322
CCTGGGA66600.051.7874454
TCACGCA4650.047.494964
CTGGGGG25800.046.8007625
ACAACGC3350.043.4833
GTACACG3900.043.4674531
ACCCGGG9100.038.727773
CTGGGAA62350.038.2041975
CACGCAG6700.035.0618445
Produced by FastQC (version 0.11.5)