##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211542_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2754483 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.53477258708803 31.0 31.0 34.0 30.0 34.0 2 31.481554614786152 31.0 31.0 34.0 29.0 34.0 3 31.5727655607241 33.0 31.0 34.0 28.0 34.0 4 35.16557335804941 37.0 35.0 37.0 32.0 37.0 5 35.28790302935251 37.0 35.0 37.0 33.0 37.0 6 35.447869890647354 37.0 35.0 37.0 33.0 37.0 7 35.41613870915159 37.0 35.0 37.0 33.0 37.0 8 35.3989213220775 37.0 35.0 37.0 33.0 37.0 9 36.99440512067056 39.0 37.0 39.0 33.0 39.0 10-11 36.932527810119 39.0 37.0 39.0 33.0 39.0 12-13 36.86518976519369 39.0 37.0 39.0 33.0 39.0 14-15 37.99905317985262 40.0 38.0 41.0 33.0 41.0 16-17 37.9197571740323 40.0 37.5 41.0 33.0 41.0 18-19 37.98908270626466 40.0 38.0 41.0 33.0 41.0 20-21 37.966355392282324 40.0 37.5 41.0 33.0 41.0 22-23 37.806682415538596 40.0 37.0 41.0 33.0 41.0 24-25 37.74720864133124 40.0 37.0 41.0 33.0 41.0 26-27 37.563075539039446 40.0 36.5 41.0 32.0 41.0 28-29 37.36107447386678 40.0 36.0 41.0 32.0 41.0 30-31 37.05157446969177 39.0 36.0 41.0 31.0 41.0 32-33 36.53964518931502 39.0 35.0 41.0 30.0 41.0 34-35 36.30382525504786 39.0 35.0 41.0 30.0 41.0 36-37 36.12892927638326 39.0 35.0 40.5 29.0 41.0 38-39 35.98406034816697 39.0 35.0 40.0 28.0 41.0 40-41 35.8456984486744 39.0 35.0 40.0 27.0 41.0 42-43 35.758603883196955 39.0 35.0 40.0 27.0 41.0 44-45 35.49872698433789 38.5 35.0 40.0 25.0 41.0 46-47 35.20784662675355 38.5 35.0 40.0 23.5 41.0 48-49 35.04101822374652 39.0 34.5 40.0 22.5 41.0 50-51 34.04357460183998 38.0 33.0 40.0 21.0 40.5 52-53 34.26962210331304 38.0 33.5 39.5 20.5 40.5 54-55 34.90380699390775 39.0 34.0 40.5 22.0 41.0 56-57 34.86647657654812 39.0 34.0 41.0 21.0 41.0 58-59 34.72809380199479 39.0 34.0 41.0 20.5 41.0 60-61 34.47709134527241 38.0 34.0 40.0 20.0 41.0 62-63 34.04325748243863 38.0 33.0 40.0 19.5 41.0 64-65 33.84560224187261 37.0 33.0 40.0 19.0 41.0 66-67 33.54407995983276 37.0 33.0 39.5 18.5 41.0 68-69 33.25517002646232 36.0 33.0 39.0 18.0 41.0 70-71 32.804274704182234 36.0 32.0 39.0 17.5 40.5 72-73 32.404050233746226 35.0 31.5 38.5 16.0 40.0 74-75 32.01787540529384 35.0 31.0 37.0 14.5 39.5 76-77 31.560713026727704 35.0 31.0 37.0 13.0 39.0 78-79 31.225860352015243 35.0 30.5 36.0 12.5 39.0 80-81 30.955332997154095 35.0 30.5 36.0 11.5 37.5 82-83 30.680044494738212 35.0 30.0 35.5 11.0 37.0 84-85 30.414599218800767 34.0 30.0 35.0 10.0 37.0 86-87 30.080044059084774 34.0 29.0 35.0 10.0 36.0 88-89 29.86281563545682 34.0 29.0 35.0 10.0 36.0 90-91 29.616016689883367 34.0 29.0 35.0 10.0 36.0 92-93 29.324661107002658 34.0 29.0 35.0 10.0 35.0 94-95 29.214218602910236 34.0 29.0 35.0 10.0 35.0 96-97 29.130871020078906 34.0 27.5 35.0 10.0 35.0 98-99 28.929902998130686 34.0 27.0 35.0 9.0 35.0 100 27.563375050780856 32.0 24.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26047804537354935 1101 2 0.2521637526813052 1101 3 0.2757358205957203 1101 4 0.15937703126335379 1101 5 0.17824903245115564 1101 6 0.22961696389805297 1101 7 0.20827334740860692 1101 8 0.267950886705286 1101 9 0.20784364540841693 1101 10-11 0.21040531976677812 1101 12-13 0.1913151181748134 1101 14-15 0.30133535540579715 1101 16-17 0.20096131247462523 1101 18-19 0.18398518967619282 1101 20-21 0.16254294237662492 1101 22-23 0.24850944820657617 1101 24-25 0.23624408155033905 1101 26-27 0.19797678906296312 1101 28-29 0.1531125843747887 1101 30-31 0.30622210209357803 1101 32-33 0.28315713302859535 1101 34-35 0.33895397933441274 1101 36-37 0.3475345374671761 1101 38-39 0.3748223776488544 1101 40-41 0.28384503608076983 1101 42-43 0.1125279229748557 1101 44-45 0.28447154621761683 1101 46-47 0.2702099626176917 1101 48-49 0.25824234918169253 1101 50-51 0.3041094927189292 1101 52-53 0.3174196532903295 1101 54-55 0.24838001532153697 1101 56-57 0.3173009958332358 1101 58-59 0.4210778707577063 1101 60-61 0.39382427646614104 1101 62-63 0.2820595889721744 1101 64-65 0.37714041654191277 1101 66-67 0.24064639595232507 1101 68-69 0.2680890415191399 1101 70-71 0.21357140446453826 1101 72-73 0.2903396740137012 1101 74-75 0.14985903713952808 1101 76-77 0.2193570098625699 1101 78-79 0.0989379463181308 1101 80-81 0.1780529334729195 1101 82-83 0.16367505145042216 1101 84-85 0.13668218241355845 1101 86-87 0.05918009670815394 1101 88-89 -0.11606134461389317 1101 90-91 -0.10403828486255051 1101 92-93 0.027392349857329634 1101 94-95 -0.24647433734523005 1101 96-97 -0.23345485777509722 1101 98-99 -0.22674487525278053 1101 100 -0.2877426797226015 1102 1 0.24190189980497934 1102 2 0.23589972753303456 1102 3 0.23770460697004836 1102 4 0.1840818795898329 1102 5 0.18372448200813096 1102 6 0.23029397176085098 1102 7 0.1981538614594882 1102 8 0.22194998402813582 1102 9 0.17767126574557324 1102 10-11 0.15050002168536025 1102 12-13 0.1798455756104218 1102 14-15 0.19044067565823042 1102 16-17 0.2336536658489763 1102 18-19 0.25491858952258184 1102 20-21 0.1696337089395641 1102 22-23 0.16263634561018847 1102 24-25 0.243289318344722 1102 26-27 0.1260496525290833 1102 28-29 0.1313750892246972 1102 30-31 0.07796553590912936 1102 32-33 0.07728339697455766 1102 34-35 0.22751096280908456 1102 36-37 0.1882634888572241 1102 38-39 0.16757087120738134 1102 40-41 0.10336899265725208 1102 42-43 0.05930362353942087 1102 44-45 -0.01608431286972234 1102 46-47 0.12344376391786227 1102 48-49 0.08903591325196913 1102 50-51 0.23245553479635106 1102 52-53 0.3789382888225532 1102 54-55 0.4938032838046382 1102 56-57 0.2927505528087906 1102 58-59 0.36073905009748586 1102 60-61 0.2805679874194098 1102 62-63 0.24162321583393975 1102 64-65 0.309974110154279 1102 66-67 0.10052754346085635 1102 68-69 0.09807890619674708 1102 70-71 0.09310557554561427 1102 72-73 0.14660813336551115 1102 74-75 0.10843486597926955 1102 76-77 0.10368764308266876 1102 78-79 0.05844218652527289 1102 80-81 0.044787300925865026 1102 82-83 0.1128955026306322 1102 84-85 0.11909779397732834 1102 86-87 -0.005769107906584026 1102 88-89 -0.20465237643722034 1102 90-91 -0.2851339290493904 1102 92-93 -0.19062991617904856 1102 94-95 -0.11036012493104508 1102 96-97 -0.13040713465943554 1102 98-99 -0.34607245995794855 1102 100 -0.45382735717388556 1103 1 0.3678191173482297 1103 2 0.411478710065591 1103 3 0.26891730636167566 1103 4 0.33665574202166226 1103 5 0.325249976512211 1103 6 0.26010747674925483 1103 7 0.281761187636242 1103 8 0.25477259213164416 1103 9 0.3508848516791119 1103 10-11 0.32147476808941633 1103 12-13 0.3143262740512611 1103 14-15 0.40223553756969466 1103 16-17 0.4101140509165262 1103 18-19 0.41529145106143517 1103 20-21 0.3909698175345184 1103 22-23 0.4780705262977065 1103 24-25 0.39758744054617523 1103 26-27 0.41241361010355604 1103 28-29 0.43748837386247175 1103 30-31 0.3489235793496803 1103 32-33 0.31944231924796185 1103 34-35 0.3784562863005121 1103 36-37 0.4834234839748248 1103 38-39 0.3692045495211502 1103 40-41 0.23864179702058408 1103 42-43 0.26776945634276217 1103 44-45 0.16455052822727367 1103 46-47 0.18437553231200354 1103 48-49 0.13751754962844842 1103 50-51 0.1904585027844803 1103 52-53 0.2578527246799638 1103 54-55 0.3046467192187734 1103 56-57 0.21858811006462275 1103 58-59 0.24564406805317418 1103 60-61 0.23566014280836356 1103 62-63 0.19353923847288002 1103 64-65 0.21124096569291595 1103 66-67 0.21441413033875278 1103 68-69 0.2557490997583969 1103 70-71 0.12242762795437301 1103 72-73 0.08795824675294028 1103 74-75 0.15191203752512905 1103 76-77 0.10816789182834441 1103 78-79 0.026208679095667264 1103 80-81 0.18858054393471235 1103 82-83 0.22321472195981684 1103 84-85 0.2642972980277989 1103 86-87 0.06742135084673251 1103 88-89 0.0802282294948995 1103 90-91 -0.02481760061773386 1103 92-93 -0.10351368282579898 1103 94-95 0.09432505632823052 1103 96-97 0.038893460865455864 1103 98-99 0.022606913809404716 1103 100 -0.14669197965478986 1104 1 0.2688482768679563 1104 2 0.2739525046148579 1104 3 0.24654896889899902 1104 4 0.2796199755519311 1104 5 0.24380661204997978 1104 6 0.23407355260211915 1104 7 0.3336384508342647 1104 8 0.3297868860138635 1104 9 0.3794027349139739 1104 10-11 0.2851778365094617 1104 12-13 0.30875801282262216 1104 14-15 0.3885324691496379 1104 16-17 0.380414540084935 1104 18-19 0.23944175917400656 1104 20-21 0.2880443363071592 1104 22-23 0.370974407880837 1104 24-25 0.28093665597086215 1104 26-27 0.3065150013747626 1104 28-29 0.3194907879403601 1104 30-31 0.36140055031722085 1104 32-33 0.2808678957678765 1104 34-35 0.3212299347345393 1104 36-37 0.31841490764548297 1104 38-39 0.3316592855607894 1104 40-41 0.3502166651992198 1104 42-43 0.13836217599208567 1104 44-45 0.22150053140369153 1104 46-47 0.388384172169701 1104 48-49 0.45595665769020144 1104 50-51 0.47379818895435477 1104 52-53 0.46735656814606585 1104 54-55 0.5680702628310002 1104 56-57 0.5556823226305383 1104 58-59 0.5936238660329636 1104 60-61 0.5509291854358978 1104 62-63 0.5053555144401045 1104 64-65 0.47090680649083794 1104 66-67 0.5204652071445466 1104 68-69 0.35983397365459524 1104 70-71 0.38401837822436846 1104 72-73 0.5827489207888945 1104 74-75 0.5949937985314193 1104 76-77 0.5388171517228848 1104 78-79 0.3698653718089595 1104 80-81 0.3861696424672054 1104 82-83 0.31360605669076946 1104 84-85 0.29050910159809007 1104 86-87 0.2778812962123389 1104 88-89 0.4535177282560987 1104 90-91 0.40440544747324125 1104 92-93 0.3764685292318859 1104 94-95 0.3551725247228674 1104 96-97 0.3865524405999068 1104 98-99 0.3453310101184073 1104 100 0.32424379316777774 1105 1 0.2924603007830662 1105 2 0.2692527161017928 1105 3 0.26734612978906114 1105 4 0.20300375711043017 1105 5 0.09458413199755711 1105 6 0.12391884738995884 1105 7 0.19427543559628901 1105 8 0.19305770911137898 1105 9 0.15414693119389256 1105 10-11 0.18272205698902155 1105 12-13 0.18731283789865216 1105 14-15 0.2599423135369392 1105 16-17 0.32054086034219864 1105 18-19 0.21755366457229997 1105 20-21 0.25404212147244465 1105 22-23 0.3122687627700529 1105 24-25 0.2529954006095423 1105 26-27 0.30752936589657054 1105 28-29 0.27904618563103867 1105 30-31 0.2597091038283281 1105 32-33 0.1746199916722091 1105 34-35 0.1588739480282797 1105 36-37 0.23923917497405256 1105 38-39 0.2707275803153095 1105 40-41 0.300890982167779 1105 42-43 0.4043929638961359 1105 44-45 0.30397269767605195 1105 46-47 0.23133379224235995 1105 48-49 0.31711597903194644 1105 50-51 0.424195119853259 1105 52-53 0.4191093527004526 1105 54-55 0.5571172647336482 1105 56-57 0.47960563338777007 1105 58-59 0.4700488151109141 1105 60-61 0.47771385609503625 1105 62-63 0.4354974723001064 1105 64-65 0.4070802423228983 1105 66-67 0.329082466832503 1105 68-69 0.41093941770035514 1105 70-71 0.30779682196503444 1105 72-73 0.33805352318801596 1105 74-75 0.3385864461876551 1105 76-77 0.3819457286443182 1105 78-79 0.356228848116352 1105 80-81 0.3795377226152965 1105 82-83 0.3842914123842007 1105 84-85 0.43218951645184944 1105 86-87 0.2856810143715478 1105 88-89 0.21414524821548753 1105 90-91 0.22749493393266462 1105 92-93 0.34177583282186674 1105 94-95 0.37291306246072864 1105 96-97 0.15518291792609062 1105 98-99 0.24420627016415608 1105 100 0.4531423524617537 1106 1 0.26891406461038514 1106 2 0.2615602868661995 1106 3 0.1918216079231172 1106 4 0.21208593405010134 1106 5 0.2144353089556148 1106 6 0.1559396438453362 1106 7 0.19397545950947404 1106 8 0.2860709311705705 1106 9 0.23181597550121325 1106 10-11 0.23206629558509917 1106 12-13 0.2594033174783448 1106 14-15 0.25904026280419146 1106 16-17 0.22532286121099077 1106 18-19 0.26806673687926974 1106 20-21 0.3106797055518271 1106 22-23 0.35001516411824696 1106 24-25 0.37170875553549365 1106 26-27 0.4138224845111722 1106 28-29 0.39216175566343026 1106 30-31 0.48606028149388436 1106 32-33 0.49098846277050257 1106 34-35 0.5575618002702427 1106 36-37 0.5739377886223025 1106 38-39 0.47622894600831955 1106 40-41 0.4294846815379785 1106 42-43 0.47246412944944893 1106 44-45 0.6622944871183094 1106 46-47 0.5023903094350928 1106 48-49 0.5703319372881097 1106 50-51 0.5492494197699216 1106 52-53 0.6436136257088805 1106 54-55 0.5252077212019728 1106 56-57 0.6140440908880933 1106 58-59 0.5569855758125328 1106 60-61 0.6067924814310359 1106 62-63 0.6225289660159348 1106 64-65 0.6081199353850977 1106 66-67 0.6623869109593556 1106 68-69 0.5012977216846153 1106 70-71 0.45351179798817753 1106 72-73 0.507074011649074 1106 74-75 0.6231312159677955 1106 76-77 0.4758052865380513 1106 78-79 0.5157328782196124 1106 80-81 0.5477487237194509 1106 82-83 0.4513330609508941 1106 84-85 0.5012012909849197 1106 86-87 0.5570979981119955 1106 88-89 0.5124843782565343 1106 90-91 0.5059974377425007 1106 92-93 0.43479996203043925 1106 94-95 0.426169194401151 1106 96-97 0.3839008290926067 1106 98-99 0.3554034789715672 1106 100 0.3788827061788389 1107 1 0.24187557376220425 1107 2 0.23810128673679642 1107 3 0.3260456801599574 1107 4 0.24199074936016984 1107 5 0.2668666882709232 1107 6 0.2699139641087456 1107 7 0.23624062507619925 1107 8 0.22462576301574444 1107 9 0.2834471995303147 1107 10-11 0.379476394939509 1107 12-13 0.36497996128343146 1107 14-15 0.3797458085345369 1107 16-17 0.43112812081214 1107 18-19 0.34056598201579646 1107 20-21 0.3479570208259517 1107 22-23 0.503830459809059 1107 24-25 0.4483507633285626 1107 26-27 0.4166740711623049 1107 28-29 0.4129143904964252 1107 30-31 0.4052812623850528 1107 32-33 0.44942675807309485 1107 34-35 0.5199922449926717 1107 36-37 0.5396234922269585 1107 38-39 0.532864195975975 1107 40-41 0.45080957477870953 1107 42-43 0.4648691244889136 1107 44-45 0.40915569288690534 1107 46-47 0.3855514620037823 1107 48-49 0.44091418865045995 1107 50-51 0.39721847755651396 1107 52-53 0.4226974371224159 1107 54-55 0.48458636072231087 1107 56-57 0.4129168280949571 1107 58-59 0.32725653585509207 1107 60-61 0.4183613034126594 1107 62-63 0.36649904466552385 1107 64-65 0.4190916963018694 1107 66-67 0.5202080155043589 1107 68-69 0.49393852169611563 1107 70-71 0.5355500012693142 1107 72-73 0.3766371973167324 1107 74-75 0.4543013262497624 1107 76-77 0.4885614009322161 1107 78-79 0.5201348106701147 1107 80-81 0.6355752920381939 1107 82-83 0.6663580792832953 1107 84-85 0.5292106800487453 1107 86-87 0.6085850791627649 1107 88-89 0.41139773430766624 1107 90-91 0.6610812635343883 1107 92-93 0.5712645409603994 1107 94-95 0.6553263354096508 1107 96-97 0.5933089442344475 1107 98-99 0.4836824621345137 1107 100 0.7368332158914157 1108 1 0.14310735259846652 1108 2 0.22774464112097093 1108 3 0.17363299631001183 1108 4 0.1498480819163106 1108 5 0.15597980520943366 1108 6 0.17066425239374183 1108 7 0.15318293013748274 1108 8 0.13753058355474224 1108 9 0.17089314968661284 1108 10-11 0.1483352347404292 1108 12-13 0.18958455383872064 1108 14-15 0.26375699962581933 1108 16-17 0.35075173500346324 1108 18-19 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2316 88-89 0.03390458511665173 2316 90-91 0.05456203138187732 2316 92-93 0.1092197630055054 2316 94-95 0.16970352088152296 2316 96-97 -0.001782140780552055 2316 98-99 0.20509828825738552 2316 100 -0.04822749585020958 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 12.0 1 17.0 2 36.0 3 32.0 4 62.0 5 44.0 6 198.0 7 1823.0 8 2890.0 9 2971.0 10 3479.0 11 3916.0 12 4520.0 13 5061.0 14 5855.0 15 7052.0 16 10619.0 17 17506.0 18 26258.0 19 30599.0 20 34011.0 21 38850.0 22 42975.0 23 41317.0 24 35996.0 25 31988.0 26 32325.0 27 34777.0 28 39974.0 29 46215.0 30 55523.0 31 67740.0 32 85971.0 33 113335.0 34 166956.0 35 221519.0 36 325668.0 37 539277.0 38 550584.0 39 126142.0 40 390.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.38676381387649 15.69033381383286 11.004045214472006 20.91885715781864 2 14.711361231277328 20.704760326542004 43.665762512791886 20.91811592938878 3 18.675846661308967 34.33161356720797 28.421716626072342 18.570823145410724 4 11.072592356944652 17.485674400801557 35.81808350192854 35.62364974032525 5 9.764166342564701 38.419391937505154 33.60510690280899 18.211334817121152 6 24.989171953016623 32.24750319577146 26.25851313216099 16.504811719050938 7 26.12176450043313 32.82668316411109 24.422534986272705 16.62901734918308 8 29.357314195696066 32.08996054623156 23.173706588419897 15.379018669652474 9 32.505005727314476 14.343845814522146 21.14499091435532 32.00615754380806 10-11 24.03795936379563 27.022384276021366 29.41995918117651 19.5196971790065 12-13 26.28895942164111 24.720942999176554 28.586078519364072 20.40401905981826 14-15 24.66559719739987 24.67442068195732 29.221365613972917 21.4386165066699 16-17 21.017827114692714 26.15018664726399 32.46881784377979 20.363168394263504 18-19 21.46522821263378 24.911951486217518 34.05200358820728 19.570816712941422 20-21 21.164536057617678 26.010200270775048 34.177225301943956 18.64803836966332 22-23 19.644967297946895 26.668222393937384 31.435521612048227 22.25128869606749 24-25 19.28272671255583 26.735631132156655 31.70252143497504 22.279120720312473 26-27 20.36305332519311 24.317670661077432 32.47443426432169 22.844841749407774 28-29 20.220275963196677 26.36696997174422 32.90460034287503 20.508153722184073 30-31 23.282206595254806 25.500359286307432 33.005231165064366 18.21220295337339 32-33 20.00741482707603 25.710586081803115 33.986088666008335 20.295910425112513 34-35 19.128699094500956 27.676926713656126 33.15881437251426 20.035559819328665 36-37 21.599863467472304 26.56053320309486 32.08962852077355 19.749974808659285 38-39 22.43691833970595 27.070302145629764 30.623009691463597 19.869769823200688 40-41 20.673841253194684 27.522628780511543 31.71836745958485 20.085162506708926 42-43 21.944707335869197 26.68730293735469 30.039955461390793 21.328034265385323 44-45 21.33742630962984 26.866576054635967 31.25808176691644 20.53791586881775 46-47 20.213967337527347 25.716822353549933 33.192679539203134 20.87653076971958 48-49 21.24369357348133 26.65408558404 32.06229905215249 20.039921790326183 50-51 19.692966666648513 27.94627116364007 32.61064566428246 19.750116505428963 52-53 19.021281277733635 29.053704669346043 28.987761249961874 22.93725280295845 54-55 19.241776482426513 28.724599904349624 29.801265824163814 22.23235778906005 56-57 21.369381617031106 26.88377845389189 29.03980903225994 22.70703089681707 58-59 20.44501940491591 27.93686934023286 30.595837588797348 21.02227366605388 60-61 22.430796739889868 28.195689403480028 30.208474049412953 19.165039807217155 62-63 19.580518260884094 28.350958542725202 31.09476314344697 20.97376005294373 64-65 18.833646556811477 28.27570929229652 30.813854464569427 22.076789686322577 66-67 21.190913233801577 26.960704747276132 30.08970930264226 21.758672716280035 68-69 21.357541551511854 27.491243210776112 29.947846922009695 21.20336831570234 70-71 19.85258733060237 28.312617424752823 30.63487180567445 21.199923438970362 72-73 20.84755962694999 28.44192167460651 29.018170552816123 21.692348145627367 74-75 21.847485257523235 27.807980341064088 29.02387942558048 21.3206549758322 76-77 20.20404250428576 27.01655877673993 31.999533749932368 20.779864969041938 78-79 20.790965272835543 27.316869036034337 31.079014829123757 20.81315086200636 80-81 18.888027882095166 28.644891102814135 30.720742573574324 21.746338441516375 82-83 19.456281771968044 29.344172113289762 28.237726942628903 22.96181917211329 84-85 19.54814500928285 30.258372993783954 27.38357583488471 22.80990616204848 86-87 19.967486281799964 28.194575394406762 28.767559777182626 23.07037854661065 88-89 19.40242469487973 27.780160174524582 30.031797431804545 22.78561769879114 90-91 21.72227030848868 27.699650456117453 29.52279479680572 21.05528443858815 92-93 18.983925760362556 28.552356120757434 30.05813816256534 22.40557995631467 94-95 18.43035070700777 28.763178042716948 29.838300443487764 22.968170806787523 96-97 20.469326210909227 27.324042157227442 29.64496625689853 22.561665374964804 98-99 21.031047549088402 27.256715767022012 28.811698536539136 22.90053814735045 100 18.980916009740845 29.02960206729608 29.809414332695926 22.180067590267146 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2235.0 1 1805.5 2 5189.5 3 7467.0 4 4426.0 5 3216.0 6 4118.0 7 6450.0 8 9390.0 9 10704.5 10 9587.0 11 8339.0 12 8576.0 13 9062.5 14 9606.0 15 10103.5 16 10673.5 17 11302.0 18 11808.0 19 12188.5 20 12560.0 21 13282.5 22 14474.5 23 16018.5 24 17666.5 25 19506.0 26 22186.5 27 25255.5 28 27468.5 29 30702.0 30 36042.5 31 40176.5 32 44922.5 33 52177.5 34 59435.0 35 64625.5 36 71625.0 37 78121.0 38 78394.5 39 77604.0 40 73695.0 41 68790.0 42 65523.0 43 63265.0 44 64405.0 45 67373.0 46 70814.5 47 75961.5 48 89008.0 49 120984.0 50 204746.5 51 217116.5 52 132698.0 53 87485.5 54 72666.0 55 61923.5 56 53444.0 57 48255.0 58 41394.5 59 30955.5 60 20756.0 61 14440.0 62 11120.5 63 8474.0 64 6239.5 65 4664.5 66 3539.5 67 2831.5 68 2326.5 69 1860.0 70 1523.0 71 1273.5 72 1091.0 73 942.0 74 814.0 75 678.5 76 601.0 77 548.0 78 462.5 79 405.5 80 369.0 81 299.0 82 246.5 83 230.0 84 203.5 85 161.5 86 127.0 87 98.5 88 72.5 89 58.5 90 43.5 91 25.0 92 17.5 93 13.5 94 10.5 95 6.5 96 6.0 97 6.5 98 4.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1658024391510131 2 0.09943789814640352 3 0.029551825151943215 4 0.03071356766405892 5 0.026139206522603334 6 0.0022508761172241763 7 0.004247621059923041 8 0.0043565344204338895 9 0.005010014583498972 10-11 0.004229468833171234 12-13 0.007515021875248459 14-15 0.017026788693195783 16-17 0.008114045358058118 18-19 0.016754505291918666 20-21 0.019477339304689846 22-23 0.04412806323364493 24-25 0.016554830797648776 26-27 0.009421005684188286 28-29 0.025776161987567176 30-31 0.014957434843489685 32-33 0.019658861572207923 34-35 0.019313969263923574 36-37 0.020348646188776624 38-39 0.018624184647354873 40-41 0.025013768463991247 42-43 0.03329118386281563 44-45 0.03577803892781332 46-47 0.020257885055017584 48-49 0.012633949819258278 50-51 0.01159927289440523 52-53 0.022036803276694755 54-55 0.02555833526654548 56-57 0.018515271286844028 58-59 0.024342136074174354 60-61 0.02581246644107079 62-63 0.049791557980209 64-65 0.031149221106102305 66-67 0.021347018660126057 68-69 0.027682145796507 70-71 0.040806205738064094 72-73 0.038918374155876075 74-75 0.04100588023233398 76-77 0.021637454288154983 78-79 0.016082872902101773 80-81 0.021818976555673063 82-83 0.017535051042246404 84-85 0.017426137681735558 86-87 0.02063908181680555 88-89 0.017680268856260867 90-91 0.027282796807967233 92-93 0.02534050854552379 94-95 0.018787554688121145 96-97 0.013232973302067939 98-99 0.01806146561804883 100 0.04095142355207856 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2754483.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.915725367694904 #Duplication Level Percentage of deduplicated Percentage of total 1 82.71243400893891 47.903506125552944 2 12.096711052367633 14.011795903225668 3 2.5936081522135974 4.50632092365052 4 0.8681602490613111 2.0112052223913803 5 0.4317869956408848 1.2503628528439779 6 0.26054967685369274 0.9053954117580074 7 0.16888567527818485 0.6846795471564338 8 0.1175810694261496 0.54478343402598 9 0.08907855052565687 0.4643143981556651 >10 0.531848932682316 5.817964475254672 >50 0.0682164172752026 2.7951678016503663 >100 0.052450006130287524 6.022725284074196 >500 0.005068707936944385 1.9697353634283474 >1k 0.002764749783786221 3.1474743958085325 >5k 3.291368790221692E-4 1.3681322969062357 >10k+ 5.266190064354707E-4 6.5964365641170515 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 45756 1.661146574511442 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22966 0.833768079164039 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20235 0.7346206166456646 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 19403 0.7044153113306562 No Hit GTACCTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 19154 0.695375502408256 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 16341 0.5932510747025849 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15611 0.5667488236449454 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13558 0.49221578060202226 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 8788 0.31904353738977514 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 8583 0.3116011244215339 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 6883 0.24988355346538715 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6369 0.23122306436452866 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 5263 0.19107033878952964 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 4167 0.1512806577495668 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 3828 0.13897344801184106 No Hit GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 3725 0.13523408930096864 No Hit GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG 3615 0.13124059941557092 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 3605 0.13087755488053476 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 3283 0.11918752085237048 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3081 0.11185402124464011 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3069 0.11141836780259672 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 3062 0.1111642366280714 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 2813 0.10212442770567108 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 7.260890700723148E-5 10-11 0.0 0.0 0.0 1.815222675180787E-5 7.260890700723148E-5 12-13 0.0 0.0 0.0 7.260890700723148E-5 7.260890700723148E-5 14-15 0.0 3.630445350361574E-5 0.0 7.260890700723148E-5 7.260890700723148E-5 16-17 0.0 3.630445350361574E-5 0.0 7.260890700723148E-5 7.260890700723148E-5 18-19 0.0 3.630445350361574E-5 0.0 7.260890700723148E-5 7.260890700723148E-5 20-21 0.0 3.630445350361574E-5 0.0 1.4521781401446296E-4 7.260890700723148E-5 22-23 0.0 3.630445350361574E-5 0.0 8.713068840867778E-4 9.076113375903935E-5 24-25 0.0 3.630445350361574E-5 0.0 0.0018878315821880187 1.0891336051084723E-4 26-27 0.0 3.630445350361574E-5 0.0 0.0029588129605446828 1.0891336051084723E-4 28-29 0.0 3.630445350361574E-5 0.0 0.005010014583498972 1.0891336051084723E-4 30-31 0.0 3.630445350361574E-5 0.0 0.013668626744111326 1.0891336051084723E-4 32-33 0.0 3.630445350361574E-5 0.0 0.03104030774559146 1.0891336051084723E-4 34-35 0.0 3.630445350361574E-5 0.0 0.05336754665031514 1.4521781401446296E-4 36-37 0.0 5.4456680255423614E-5 0.0 0.086731339420138 1.4521781401446296E-4 38-39 0.0 7.260890700723148E-5 0.0 0.15325925046551386 1.4521781401446296E-4 40-41 0.0 9.076113375903935E-5 0.0 0.2719566611955855 1.4521781401446296E-4 42-43 0.0 1.0891336051084723E-4 0.0 0.36400660305400323 1.8152226751807872E-4 44-45 0.0 1.0891336051084723E-4 0.0 0.4559113270984065 1.8152226751807872E-4 46-47 0.0 1.0891336051084723E-4 0.0 0.5565835766639329 1.8152226751807872E-4 48-49 0.0 1.0891336051084723E-4 0.0 0.6746638116844432 2.1782672102169446E-4 50-51 0.0 1.270655872626551E-4 0.0 0.8195911900708772 2.1782672102169446E-4 52-53 0.0 1.4521781401446296E-4 0.0 0.9880801587811578 2.1782672102169446E-4 54-55 0.0 1.4521781401446296E-4 0.0 1.1795135421057235 2.1782672102169446E-4 56-57 0.0 1.4521781401446296E-4 0.0 1.3690772460748533 2.1782672102169446E-4 58-59 0.0 1.4521781401446296E-4 0.0 1.5843263508977912 2.1782672102169446E-4 60-61 0.0 1.4521781401446296E-4 0.0 1.8028247043093022 2.1782672102169446E-4 62-63 0.0 1.4521781401446296E-4 0.0 2.0561753330842847 2.904356280289259E-4 64-65 0.0 1.4521781401446296E-4 0.0 2.3039350760197106 2.904356280289259E-4 66-67 0.0 1.4521781401446296E-4 0.0 2.5712266149400813 3.085878547807338E-4 68-69 0.0 1.4521781401446296E-4 0.0 2.927736348345588 3.267400815325417E-4 70-71 0.0 1.4521781401446296E-4 0.0 3.3788010309012617 3.267400815325417E-4 72-73 0.0 1.4521781401446296E-4 0.0 3.7411920857743537 3.267400815325417E-4 74-75 0.0 1.4521781401446296E-4 0.0 4.1108621835749215 3.267400815325417E-4 76-77 0.0 1.4521781401446296E-4 0.0 4.507216054700646 3.267400815325417E-4 78-79 0.0 1.4521781401446296E-4 0.0 4.947298640071477 3.267400815325417E-4 80-81 0.0 1.4521781401446296E-4 0.0 5.3516757954214995 3.267400815325417E-4 82-83 0.0 1.6337004076627084E-4 0.0 5.826538047248794 3.267400815325417E-4 84-85 0.0 1.8152226751807872E-4 0.0 6.305629767909259 3.267400815325417E-4 86-87 0.0 1.8152226751807872E-4 0.0 6.7978273962845295 3.267400815325417E-4 88 0.0 1.8152226751807872E-4 0.0 7.1922026746943075 3.267400815325417E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 9155 0.0 72.36415 2 TATAACG 910 0.0 72.3365 2 ACCTGGG 9035 0.0 70.263504 3 TAACGCA 1025 0.0 66.47323 4 GTACCTG 10940 0.0 60.992836 1 TACCCGG 620 0.0 59.15259 2 CCTGGGG 3625 0.0 57.94315 4 GTACCCG 685 0.0 57.745083 1 ATAACGC 1255 0.0 54.665302 3 ATCACGC 415 0.0 54.34953 3 GTATAAC 1305 0.0 53.043617 1 TATCACG 435 0.0 51.882732 2 CCTGGGA 6660 0.0 51.787445 4 TCACGCA 465 0.0 47.49496 4 CTGGGGG 2580 0.0 46.800762 5 ACAACGC 335 0.0 43.483 3 GTACACG 390 0.0 43.467453 1 ACCCGGG 910 0.0 38.72777 3 CTGGGAA 6235 0.0 38.204197 5 CACGCAG 670 0.0 35.061844 5 >>END_MODULE