##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211542_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2754483 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.055456141860375 33.0 31.0 34.0 30.0 34.0 2 32.21922480552612 33.0 31.0 34.0 30.0 34.0 3 32.20923853950088 34.0 31.0 34.0 30.0 34.0 4 35.90930748165808 37.0 35.0 37.0 35.0 37.0 5 35.86712533713223 37.0 35.0 37.0 35.0 37.0 6 35.93055248480386 37.0 35.0 37.0 35.0 37.0 7 35.87104767028876 37.0 35.0 37.0 35.0 37.0 8 35.86974797085333 37.0 35.0 37.0 35.0 37.0 9 37.49826228733305 39.0 37.0 39.0 35.0 39.0 10-11 37.431137531072075 39.0 37.0 39.0 34.0 39.0 12-13 37.33064498855139 39.0 37.0 39.0 34.0 39.0 14-15 38.4407170056958 40.0 38.0 41.0 34.0 41.0 16-17 38.329464186201186 40.0 38.0 41.0 33.5 41.0 18-19 38.37852965511132 40.0 38.0 41.0 34.0 41.0 20-21 38.3505167757434 40.0 37.5 41.0 34.0 41.0 22-23 38.233660545372764 40.0 37.0 41.0 34.0 41.0 24-25 38.14582863644466 40.0 37.0 41.0 34.0 41.0 26-27 37.900700058776906 40.0 37.0 41.0 33.0 41.0 28-29 37.88710767138515 40.0 37.0 41.0 33.0 41.0 30-31 37.65129844693178 39.5 36.5 41.0 33.0 41.0 32-33 37.09494758181481 39.0 35.0 41.0 31.5 41.0 34-35 36.88652698165137 39.0 35.0 41.0 31.0 41.0 36-37 36.77126560592315 39.0 35.0 41.0 31.0 41.0 38-39 36.62506557491914 39.0 35.0 41.0 30.0 41.0 40-41 36.42022368625982 39.0 35.0 41.0 30.0 41.0 42-43 36.263622610849296 39.0 35.0 41.0 29.5 41.0 44-45 35.97801729035903 39.0 35.0 41.0 27.5 41.0 46-47 35.506741737015616 39.0 35.0 40.0 24.5 41.0 48-49 35.17383135056561 39.0 34.5 40.0 23.0 41.0 50-51 34.93160549547774 38.0 34.0 40.0 23.0 41.0 52-53 34.802687110430526 38.0 34.0 40.0 22.0 41.0 54-55 34.6694031148495 38.0 34.0 40.0 22.0 41.0 56-57 34.450258723687895 38.0 33.5 40.0 20.5 41.0 58-59 34.22426114083841 38.0 33.0 40.0 20.0 41.0 60-61 33.84890376887424 37.5 33.0 40.0 19.5 41.0 62-63 33.33657641016481 37.0 32.0 40.0 18.0 41.0 64-65 33.142221062900006 36.0 32.0 39.0 18.0 41.0 66-67 32.79699021558673 36.0 31.0 39.0 18.0 40.0 68-69 32.374524003234 35.5 31.0 39.0 16.0 40.0 70-71 31.965168599697293 35.0 30.5 38.0 15.0 40.0 72-73 31.541388166127724 35.0 30.0 37.0 13.5 39.0 74-75 31.103608190720365 34.5 29.5 37.0 12.5 39.0 76-77 29.50412545657388 32.5 27.5 35.0 10.5 37.5 78-79 30.29670667780487 34.0 29.0 36.0 11.0 37.5 80-81 30.30897504177735 34.0 29.0 35.0 11.0 37.0 82-83 30.100309023508224 34.0 29.0 35.0 10.0 37.0 84-85 29.823446541510695 34.0 29.0 35.0 10.0 36.0 86-87 29.48460309974685 34.0 29.0 35.0 10.0 36.0 88-89 29.251097937435084 34.0 27.5 35.0 10.0 35.5 90-91 28.944195335385988 33.5 27.0 35.0 10.0 35.0 92-93 28.624735385914526 33.0 26.0 35.0 10.0 35.0 94-95 28.541247123325867 33.0 26.0 35.0 10.0 35.0 96-97 28.34216784057117 33.0 25.5 35.0 8.0 35.0 98-99 28.07928638514015 33.0 25.0 35.0 8.0 35.0 100 26.449055594098784 31.0 20.0 34.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.39432869173344187 1101 2 0.317939916060503 1101 3 0.3426187439745334 1101 4 0.23322648251758693 1101 5 0.2173944886383694 1101 6 0.20782822210379237 1101 7 0.23874346361093757 1101 8 0.18386741372134452 1101 9 0.20824754076855356 1101 10-11 0.26139115703922755 1101 12-13 0.303773142359951 1101 14-15 0.3598960358955807 1101 16-17 0.3222018252834502 1101 18-19 0.3066721706877331 1101 20-21 0.28513429555478353 1101 22-23 0.3174880958548627 1101 24-25 0.28910259438151087 1101 26-27 0.2740351394439742 1101 28-29 0.38243377589118666 1101 30-31 0.3849430108260279 1101 32-33 0.6180655191264677 1101 34-35 0.575722336741272 1101 36-37 0.5444503647518033 1101 38-39 0.5756745897069848 1101 40-41 0.512771887170004 1101 42-43 0.594031007508022 1101 44-45 0.6732452610197939 1101 46-47 0.7212521108594174 1101 48-49 0.7201256939794476 1101 50-51 0.6617893701805997 1101 52-53 0.6598764403260233 1101 54-55 0.7375451482453741 1101 56-57 0.7718832683978079 1101 58-59 0.7022992459307744 1101 60-61 0.5662953689790768 1101 62-63 0.37251645359086183 1101 64-65 0.5184413452194576 1101 66-67 0.478732999634488 1101 68-69 0.6153399841483562 1101 70-71 0.6456761166185565 1101 72-73 0.4488311623846677 1101 74-75 0.5486478077738433 1101 76-77 0.4115006724669428 1101 78-79 0.523902738092449 1101 80-81 0.5875663520099685 1101 82-83 0.6421583650885303 1101 84-85 0.6048627518923908 1101 86-87 0.6567121429387051 1101 88-89 0.48983130317262535 1101 90-91 0.5357815489588837 1101 92-93 0.5045214728037912 1101 94-95 0.44989427924294034 1101 96-97 0.39605443958308584 1101 98-99 0.24731381156619747 1101 100 0.08990266456965657 1102 1 0.19784992444203198 1102 2 0.12416126199964594 1102 3 0.2539742641620393 1102 4 0.13323519256027083 1102 5 0.13776094388854432 1102 6 0.13767278743307543 1102 7 0.16495752478711267 1102 8 0.17832861839942638 1102 9 0.09291469836490052 1102 10-11 0.1647136424103195 1102 12-13 0.13408963363801973 1102 14-15 0.27251055316125417 1102 16-17 0.0917143004400387 1102 18-19 0.060825277401598044 1102 20-21 0.19951853512846895 1102 22-23 0.19534558372019006 1102 24-25 0.15126616898302103 1102 26-27 0.11244405219310494 1102 28-29 0.23985037723253555 1102 30-31 0.2934143191536194 1102 32-33 0.4825412141482772 1102 34-35 0.554434200611226 1102 36-37 0.5825271081460315 1102 38-39 0.6710218432721646 1102 40-41 0.6087545779397274 1102 42-43 0.5035455414019765 1102 44-45 0.5683704084966479 1102 46-47 0.7399054590790044 1102 48-49 0.8290586345690443 1102 50-51 0.6679101547179016 1102 52-53 0.6642552163274829 1102 54-55 0.6571059245477215 1102 56-57 0.6976044203405394 1102 58-59 0.8273358677011373 1102 60-61 0.82079291828979 1102 62-63 0.8236471754950401 1102 64-65 0.8968531085280063 1102 66-67 0.8240842123099767 1102 68-69 0.7303105679587674 1102 70-71 0.689596506828476 1102 72-73 0.75290865009163 1102 74-75 0.7034331844040018 1102 76-77 0.7345264621114893 1102 78-79 0.5917638683392603 1102 80-81 0.6322290754027691 1102 82-83 0.6569456420916424 1102 84-85 0.6197331702119833 1102 86-87 0.4801120685074345 1102 88-89 0.5449678528871758 1102 90-91 0.6200531914353853 1102 92-93 0.47095217649605914 1102 94-95 0.6873419200091071 1102 96-97 0.6408422708616435 1102 98-99 0.6526910751875157 1102 100 0.5155307774201354 1103 1 0.34174980944855093 1103 2 0.1987901028782204 1103 3 0.26636786307249594 1103 4 0.15067565613174594 1103 5 0.14016231610123953 1103 6 0.15975025063837478 1103 7 0.22147778355456182 1103 8 0.18040364040237478 1103 9 0.23183710025104887 1103 10-11 0.15925305819204993 1103 12-13 0.23039699386313117 1103 14-15 0.2684510906112294 1103 16-17 0.24802256005548173 1103 18-19 0.31952270321794884 1103 20-21 0.34373256433295296 1103 22-23 0.3478815432733171 1103 24-25 0.2981828661647157 1103 26-27 0.29314506272239527 1103 28-29 0.4037270065745062 1103 30-31 0.485467563061853 1103 32-33 0.5640825122255109 1103 34-35 0.6604883824551067 1103 36-37 0.7334467347705527 1103 38-39 0.7429223193066292 1103 40-41 0.7464409605275364 1103 42-43 0.615765686460783 1103 44-45 0.6041153927894385 1103 46-47 0.5857840028375492 1103 48-49 0.6652391807559468 1103 50-51 0.847241522240914 1103 52-53 0.7428580776736098 1103 54-55 0.889717818342568 1103 56-57 0.8441112524950256 1103 58-59 0.8998646321315249 1103 60-61 0.7157794595557547 1103 62-63 0.8958553715107342 1103 64-65 0.7522613353799485 1103 66-67 0.7577143444561116 1103 68-69 0.8864922705046823 1103 70-71 0.8352495605798929 1103 72-73 0.8819750186746411 1103 74-75 0.821517354491565 1103 76-77 0.835346192165904 1103 78-79 0.7468361086563569 1103 80-81 0.8543939079555614 1103 82-83 0.7886234547743314 1103 84-85 0.5973621203022468 1103 86-87 0.681937188479683 1103 88-89 0.7810282662212806 1103 90-91 0.7723944972216792 1103 92-93 0.8142546093469321 1103 94-95 0.7170063831694371 1103 96-97 0.8764086619294105 1103 98-99 0.9272319042969279 1103 100 0.6903786840891151 1104 1 0.20960445654847604 1104 2 0.2026780388416256 1104 3 0.22306057636897947 1104 4 0.05701578700018217 1104 5 0.06857855395143986 1104 6 0.09351557685652523 1104 7 0.13021928663568616 1104 8 0.13764905047796816 1104 9 0.047554017854757547 1104 10-11 0.16719410386894396 1104 12-13 0.1440111225715981 1104 14-15 0.17866327535171678 1104 16-17 0.20986742378713785 1104 18-19 0.24941279914578018 1104 20-21 0.10500884919414233 1104 22-23 0.17094551633729083 1104 24-25 0.18108738303150318 1104 26-27 0.28551530603603936 1104 28-29 0.33793067851402014 1104 30-31 0.39405342164771895 1104 32-33 0.3882963487034985 1104 34-35 0.5075756069008222 1104 36-37 0.4006025698509248 1104 38-39 0.4742227449466796 1104 40-41 0.6252510742433088 1104 42-43 0.49154867356452314 1104 44-45 0.5426453464137353 1104 46-47 0.6617607514558088 1104 48-49 0.6721351848217623 1104 50-51 0.650151121376922 1104 52-53 0.5075553650124078 1104 54-55 0.534842016296956 1104 56-57 0.5894482704735537 1104 58-59 0.644296370902893 1104 60-61 0.5611990644093154 1104 62-63 0.49900955485360043 1104 64-65 0.6550502306216757 1104 66-67 0.5797205702359278 1104 68-69 0.5408407871173537 1104 70-71 0.5031867945548605 1104 72-73 0.5569734304655078 1104 74-75 0.6142973557621332 1104 76-77 0.5962921295269368 1104 78-79 0.7140904127031114 1104 80-81 0.5877717111446827 1104 82-83 0.41680146125422723 1104 84-85 0.5072820745618252 1104 86-87 0.6710547794840629 1104 88-89 0.6694788093888029 1104 90-91 0.4815711696058962 1104 92-93 0.649284150036852 1104 94-95 0.606365381962398 1104 96-97 0.6644939169957986 1104 98-99 0.5298111559327836 1104 100 0.7127724328979568 1105 1 0.1724961676264556 1105 2 0.15836574714704454 1105 3 0.16191925115008843 1105 4 0.06973749793399975 1105 5 0.09278292562679269 1105 6 0.07672163754423167 1105 7 0.0713870896694786 1105 8 0.06539207671449532 1105 9 0.1295123552351498 1105 10-11 0.19636968292935109 1105 12-13 0.09813920628346295 1105 14-15 0.20986516112797204 1105 16-17 0.16702448066599374 1105 18-19 0.04908026671672161 1105 20-21 0.0764872395490741 1105 22-23 0.17900767303141407 1105 24-25 0.20074439559947876 1105 26-27 0.20638341158903017 1105 28-29 0.1551513965297815 1105 30-31 0.2998596704025758 1105 32-33 0.4461736167998893 1105 34-35 0.5032773339850678 1105 36-37 0.5756771952704227 1105 38-39 0.5103738008334417 1105 40-41 0.39762050309623476 1105 42-43 0.48744454135695037 1105 44-45 0.5859107198646001 1105 46-47 0.6314306673502301 1105 48-49 0.6723222948713001 1105 50-51 0.6448982149971769 1105 52-53 0.608385526277992 1105 54-55 0.6202707813091877 1105 56-57 0.5974053808563156 1105 58-59 0.6611750326057972 1105 60-61 0.7472122721219208 1105 62-63 0.6247395321877036 1105 64-65 0.6050293986029018 1105 66-67 0.5706849331861719 1105 68-69 0.675842076268367 1105 70-71 0.7574697654488851 1105 72-73 0.5773380581466334 1105 74-75 0.7155722209566413 1105 76-77 0.6976987457193822 1105 78-79 0.7397991680827509 1105 80-81 0.7687739498481889 1105 82-83 0.7961558908173068 1105 84-85 0.8196168393136212 1105 86-87 0.7931227289682532 1105 88-89 0.7204632552086601 1105 90-91 0.6662225564653461 1105 92-93 0.684827851547464 1105 94-95 0.7377087198638854 1105 96-97 0.754893357662592 1105 98-99 0.8869313934296166 1105 100 0.9572617553953329 1106 1 0.2024350545736766 1106 2 0.20040178295362665 1106 3 0.22669498365065976 1106 4 0.12334371989083337 1106 5 0.0994066900531223 1106 6 0.10324192908702656 1106 7 0.1448643690750373 1106 8 0.14391058160726544 1106 9 0.08442219968623021 1106 10-11 0.04983608112684834 1106 12-13 0.06787752667207059 1106 14-15 0.1688272768838388 1106 16-17 0.20613616232910914 1106 18-19 0.1956294922737456 1106 20-21 0.21164273894557795 1106 22-23 0.174136952819957 1106 24-25 0.11888836195310404 1106 26-27 0.14182464292483132 1106 28-29 0.20706423932674767 1106 30-31 0.17916982209332133 1106 32-33 0.24432974256155404 1106 34-35 0.26773944761846025 1106 36-37 0.22925145043593176 1106 38-39 0.21185172547058073 1106 40-41 0.31695399726580575 1106 42-43 0.3064157530858296 1106 44-45 0.29054086963095926 1106 46-47 0.2655058283397551 1106 48-49 0.32222260942170067 1106 50-51 0.46099235654909876 1106 52-53 0.4846049455790009 1106 54-55 0.4908417122113917 1106 56-57 0.4976813140484353 1106 58-59 0.6520881980185536 1106 60-61 0.7233797380173215 1106 62-63 0.6464979981401839 1106 64-65 0.5852015570487552 1106 66-67 0.5353606264917588 1106 68-69 0.3727856335979922 1106 70-71 0.5752854089188126 1106 72-73 0.5003056965754631 1106 74-75 0.5090683997989878 1106 76-77 0.5526587748491352 1106 78-79 0.5406030190965119 1106 80-81 0.5927039643894787 1106 82-83 0.6083929106020527 1106 84-85 0.49286140142164214 1106 86-87 0.4476154840269295 1106 88-89 0.48418763284081834 1106 90-91 0.5649176604877191 1106 92-93 0.5988863534876359 1106 94-95 0.6763675602676464 1106 96-97 0.7360011726528128 1106 98-99 0.7337622946455085 1106 100 0.6947027329777811 1107 1 0.19431416139045155 1107 2 0.12150057660989688 1107 3 0.23212856285380923 1107 4 0.17173334447206656 1107 5 0.08483998168922824 1107 6 0.07020335196873617 1107 7 0.11235563422622619 1107 8 0.11657158320326744 1107 9 0.1739813594153432 1107 10-11 0.11335820222133464 1107 12-13 0.1449357877541857 1107 14-15 0.21159209018093605 1107 16-17 0.1366337251984575 1107 18-19 0.08772750949900399 1107 20-21 0.12635179299380894 1107 22-23 0.2772081687971806 1107 24-25 0.24136424226770004 1107 26-27 0.16546768595923567 1107 28-29 0.3500957710525867 1107 30-31 0.25031139134664926 1107 32-33 0.19717270220152017 1107 34-35 0.26314232904019974 1107 36-37 0.4329118193844437 1107 38-39 0.4775421020396209 1107 40-41 0.32454037521516454 1107 42-43 0.44766904213383896 1107 44-45 0.4001075061030832 1107 46-47 0.2864435844541475 1107 48-49 0.39966297520697225 1107 50-51 0.4669694374560862 1107 52-53 0.48158477275121214 1107 54-55 0.39084099329379285 1107 56-57 0.48858880373196456 1107 58-59 0.5411265019481561 1107 60-61 0.3799528016127738 1107 62-63 0.40999747333033554 1107 64-65 0.442985637337479 1107 66-67 0.41673275261865683 1107 68-69 0.5089310131145979 1107 70-71 0.5077333952306269 1107 72-73 0.46412652793177145 1107 74-75 0.5969869895702296 1107 76-77 0.5307857069401791 1107 78-79 0.5374599113595231 1107 80-81 0.5787220954912939 1107 82-83 0.4521534247719181 1107 84-85 0.47639962627031096 1107 86-87 0.5072777365395424 1107 88-89 0.5892998558778118 1107 90-91 0.4729120241737519 1107 92-93 0.5101683273477953 1107 94-95 0.7796059245863276 1107 96-97 0.9174707121141985 1107 98-99 0.8785929176594998 1107 100 0.850713603011858 1108 1 0.15873157276940475 1108 2 0.08540947970129764 1108 3 0.13121168435659314 1108 4 0.06833623778420161 1108 5 0.04542236911436959 1108 6 0.07102687556441367 1108 7 0.06365668193954832 1108 8 0.11326380749876108 1108 9 0.06225899597275486 1108 10-11 0.06069155165033635 1108 12-13 0.14171958493032122 1108 14-15 0.247575690989855 1108 16-17 0.2527714190794512 1108 18-19 0.20311434024956299 1108 20-21 0.139267880503688 1108 22-23 0.19999291561799737 1108 24-25 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74-75 -0.3735860884326989 2316 76-77 -0.42149124440921426 2316 78-79 -0.3014500152944741 2316 80-81 -0.3436051608670212 2316 82-83 -0.2382498738259038 2316 84-85 -0.3483812754608948 2316 86-87 -0.3099484805377095 2316 88-89 -0.11892482745354727 2316 90-91 -0.14791489921552525 2316 92-93 -0.168299017322731 2316 94-95 -0.07196993550634545 2316 96-97 -0.004351405847334178 2316 98-99 -0.22439846691161947 2316 100 -0.24628619511841165 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 6.0 11 23.0 12 58.0 13 206.0 14 646.0 15 2131.0 16 5533.0 17 13467.0 18 23993.0 19 31366.0 20 34541.0 21 39590.0 22 45350.0 23 44268.0 24 39128.0 25 36654.0 26 38913.0 27 44001.0 28 51402.0 29 61064.0 30 72452.0 31 87572.0 32 107698.0 33 136330.0 34 188336.0 35 243960.0 36 345074.0 37 517881.0 38 476059.0 39 66757.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.736583763099475 16.933639025488265 11.97706945783209 20.352707753580173 2 14.240505639325823 21.79468828765466 43.552829762329466 20.411976310690054 3 18.03314040999658 35.16966289605738 28.966963421532043 17.830233272413995 4 10.830140633684774 18.051698680794537 35.66792196368001 35.450238721840684 5 9.588002172498722 39.42834675170779 33.22986372508016 17.753787350713324 6 24.726852384657192 33.084561310135584 26.206269606816285 15.982316698390939 7 26.00713092075718 33.23284260603533 24.396048187627226 16.363978285580274 8 29.997386048031366 32.14580043202817 22.87429432373069 14.98251919620977 9 32.566619579790476 14.324139956572612 21.373339388916175 31.735901074720736 10-11 23.9676011796043 28.029034849734053 29.09807393982827 18.90529003083337 12-13 26.383735169176937 24.9891903489693 28.73597332058321 19.891101161270555 14-15 24.780203762375734 25.064140893227517 29.475204602823833 20.68045074157292 16-17 21.121432727015293 26.38789530099738 32.59797038810587 19.892701583881458 18-19 21.57329261390349 24.798481298868488 34.764195074241435 18.86403101298658 20-21 21.124809009886558 26.12167492951723 34.66214831452189 18.091367746074322 22-23 19.544811131526316 26.83980986631611 31.88070138751991 21.734677614637665 24-25 19.321295133040465 26.796622159197657 31.912140270319476 21.9699424374424 26-27 20.551843717615203 24.36590922663861 32.87660391243269 22.20564314331349 28-29 20.585456367960276 26.508545536957566 33.29064482757807 19.615353267504087 30-31 23.303817833717595 25.80446361196161 33.3046764510315 17.587042103289303 32-33 19.993958761284663 26.024850403100203 34.58569228456848 19.395498551046654 34-35 19.058940434378194 27.923804102129925 33.333581423583 19.683674039908887 36-37 21.819266343819514 26.573643894917716 32.56374863811337 19.043341123149403 38-39 23.151983706306908 26.782848782329623 30.918752995164173 19.1464145161993 40-41 20.799812658783935 28.104631521430328 31.78721160208508 19.30834421770066 42-43 22.234970024008025 26.77320359621136 30.298216870969352 20.69360950881126 44-45 21.66509551015933 27.115329422778338 31.458855577224387 19.76071948983795 46-47 20.4021140815447 25.94003252377289 33.379564193061526 20.27828920162089 48-49 21.342259631766588 26.63181397098348 32.59674064725588 19.429185749994055 50-51 19.817238547319302 28.116842141188553 32.85503546161091 19.210883849881238 52-53 19.16213764580712 29.294866895890582 29.116223276749842 22.426772181552455 54-55 19.367360553294034 28.831320653678418 29.8161767709085 21.985142022119057 56-57 21.55557205440093 26.966366377596003 29.150797369228847 22.327264198774216 58-59 20.857904087514576 28.01918256969683 30.686139199248615 20.436774143539978 60-61 22.66247753944507 28.512714607448135 30.137390157039622 18.687417696067165 62-63 19.753074540763706 28.668276018700052 31.394727989309075 20.183921451227175 64-65 18.821775831856737 28.53007344159662 30.75279951044987 21.895351216096774 66-67 21.302497766593245 27.147624147188992 30.306707881288965 21.2431702049288 68-69 21.89305348342479 27.278358458511416 29.93094403594875 20.897644022115045 70-71 19.894206749398087 28.902244807230204 30.30723029465012 20.896318148721587 72-73 21.001026586875483 28.52577223205039 28.85880028210716 21.614400898966966 74-75 22.036579990865533 27.99175646837317 28.86403291894212 21.107630621819183 76-77 20.16901666168676 27.243544245341006 31.933653136450985 20.65378595652125 78-79 20.543057627348794 27.248460700737205 31.491202798286942 20.717278873627055 80-81 18.85515210959101 28.838964772891686 30.743504688191813 21.562378429325495 82-83 19.127813171345352 29.616545780249794 28.239437348483488 23.01620369992137 84-85 19.2315080152389 30.302624832788915 27.32801041683309 23.137856735139092 86-87 19.768928564814495 28.20190662802525 28.669703366211944 23.35946144094831 88-89 19.36360336924407 27.9417657393856 29.875996378242363 22.818634513127964 90-91 21.46955741951485 28.103589365373242 29.280756100036477 21.14609711507543 92-93 18.406766260723224 29.044854731782983 30.031818988216823 22.51656001927697 94-95 17.641152829521246 29.236146571880784 29.380275684835738 23.742424913762232 96-97 19.722841811033128 27.642557626298526 29.531427450916308 23.103173111752035 98-99 20.71134359959789 27.214533628979094 28.5482257169328 23.525897054490216 100 17.149290443555383 30.439829860412626 29.55460000711699 22.856279688915002 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2418.0 1 1966.5 2 5047.5 3 7341.5 4 4641.0 5 3438.5 6 4385.0 7 6900.0 8 9932.5 9 11483.0 10 10566.0 11 9341.5 12 9699.0 13 10381.5 14 11146.0 15 11715.5 16 12260.5 17 12709.5 18 13045.5 19 13459.5 20 13836.5 21 14409.0 22 15452.5 23 17020.5 24 18446.0 25 20127.5 26 22722.0 27 25263.0 28 27492.0 29 30990.5 30 35861.5 31 40042.0 32 45195.0 33 51995.0 34 58713.5 35 63961.0 36 71524.0 37 78369.0 38 78724.5 39 77886.0 40 73441.0 41 67863.0 42 64512.0 43 62144.5 44 63132.5 45 66533.0 46 70261.5 47 74972.5 48 86858.0 49 119584.0 50 208470.0 51 221928.5 52 133792.0 53 87199.0 54 71654.0 55 60665.5 56 52089.0 57 46397.0 58 39703.0 59 29944.5 60 20165.5 61 13776.0 62 10314.5 63 7752.0 64 5639.0 65 4149.0 66 3062.0 67 2321.5 68 1814.5 69 1372.5 70 1067.5 71 809.5 72 587.5 73 475.0 74 394.5 75 290.5 76 190.0 77 123.0 78 92.0 79 67.0 80 44.5 81 32.0 82 25.5 83 17.5 84 13.0 85 10.5 86 7.5 87 4.0 88 2.5 89 2.0 90 3.0 91 2.5 92 1.5 93 0.5 94 1.5 95 3.0 96 1.5 97 1.5 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002831747373282028 2 0.06712693452818551 3 7.623935235759305E-4 4 7.986979770795463E-4 5 0.0018515271286844027 6 7.260890700723149E-4 7 0.0 8 0.0011980469656193195 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 5.627190293060441E-4 18-19 8.713068840867778E-4 20-21 0.003140335228062762 22-23 0.0 24-25 5.4456680255423614E-5 26-27 2.722834012771181E-4 28-29 0.0012161991923711273 30-31 0.0019785927159470577 32-33 0.002940660733792875 34-35 0.003957185431894115 36-37 0.001161742512115704 38-39 0.0015610915006554768 40-41 0.0054638202522941685 42-43 0.007097520659956877 44-45 0.005173384624265243 46-47 0.006897846165686991 48-49 0.008150349811561734 50-51 0.00860415548035693 52-53 0.004229468833171234 54-55 0.00255946397200491 56-57 0.0029588129605446828 58-59 0.0039027287516386925 60-61 0.007623935235759305 62-63 0.013523408930096863 64-65 0.004265773286674849 66-67 0.010256008114771448 68-69 0.012343514191229352 70-71 0.005391211345286938 72-73 0.007642087462511113 74-75 0.002940660733792875 76-77 0.0029043562802892595 78-79 0.008222958718568965 80-81 0.006153604868862868 82-83 0.016391460756882508 84-85 0.01738983322823194 86-87 0.022363543358227298 88-89 0.020747995177316396 90-91 0.02423322271366351 92-93 0.03407172961314338 94-95 0.038083371725292914 96-97 0.02325300246906588 98-99 0.018569727967099454 100 0.01847896683334041 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2754483.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.91101039901901 #Duplication Level Percentage of deduplicated Percentage of total 1 82.23031522407128 45.975800096076604 2 12.466949770051533 13.94079516474798 3 2.666874681624605 4.473229741715815 4 0.9026784481428284 2.018786564043361 5 0.4401469897051453 1.2304531459250647 6 0.2657050470470134 0.8913502589110442 7 0.16701397157660147 0.6536543931120582 8 0.11477037700743445 0.51335421938896 9 0.08590322596107862 0.4322642544017233 >10 0.5291065390856836 5.635779050972102 >50 0.06682654033435652 2.610911102591801 >100 0.05471980918984111 6.037874616503133 >500 0.0050895732912237725 1.9618599026197212 >1k 0.0029083275949834415 3.2650114658587337 >5k 2.643934177257674E-4 0.98558643979475 >10k+ 7.270818987458604E-4 9.373289583337284 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 54582 1.9815696811343546 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 40291 1.4627427361141818 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24873 0.9030006719954343 No Hit GTACCTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 23165 0.8409926654112587 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22369 0.8120943204223805 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 22321 0.810351706654207 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15933 0.5784388576731097 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14946 0.5426063620650409 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 13443 0.48804076844910643 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 11315 0.4107848913934121 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 10394 0.377348489716582 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 8775 0.31857157949422815 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6286 0.22820979472372854 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 6235 0.22635826759504415 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 5373 0.1950638286749274 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 4791 0.17393463673582302 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 4250 0.1542939273903669 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 3739 0.13574235165001927 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 3637 0.13203929739265047 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 3603 0.13080494597352751 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3526 0.1280095030537491 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3373 0.1224549216676959 No Hit GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 3290 0.11944165202689579 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 3131 0.11366924391982089 No Hit GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG 3127 0.11352402610580643 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2955 0.10727966010318452 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 2862 0.10390334592734825 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 2847 0.103358779124794 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 2782 0.100998989647059 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 1.815222675180787E-5 12-13 0.0 0.0 0.0 3.630445350361574E-5 3.630445350361574E-5 14-15 0.0 0.0 0.0 3.630445350361574E-5 3.630445350361574E-5 16-17 0.0 0.0 0.0 5.4456680255423614E-5 3.630445350361574E-5 18-19 0.0 0.0 0.0 7.260890700723148E-5 3.630445350361574E-5 20-21 0.0 0.0 0.0 1.4521781401446296E-4 3.630445350361574E-5 22-23 0.0 0.0 0.0 8.531546573349699E-4 3.630445350361574E-5 24-25 0.0 0.0 0.0 0.001851527128684403 3.630445350361574E-5 26-27 0.0 0.0 0.0 0.002940660733792875 3.630445350361574E-5 28-29 0.0 0.0 0.0 0.005082623490506204 3.630445350361574E-5 30-31 0.0 0.0 0.0 0.014031671279147485 3.630445350361574E-5 32-33 0.0 0.0 0.0 0.031548570094642084 3.630445350361574E-5 34-35 0.0 0.0 0.0 0.05400287458662842 3.630445350361574E-5 36-37 0.0 1.815222675180787E-5 0.0 0.08800199529276456 3.630445350361574E-5 38-39 0.0 3.630445350361574E-5 0.0 0.15478403751266573 3.630445350361574E-5 40-41 0.0 3.630445350361574E-5 0.0 0.27411677617905067 3.630445350361574E-5 42-43 0.0 3.630445350361574E-5 0.0 0.3664934581190009 3.630445350361574E-5 44-45 0.0 3.630445350361574E-5 0.0 0.45854339997741866 3.630445350361574E-5 46-47 0.0 3.630445350361574E-5 0.0 0.5590159750486752 3.630445350361574E-5 48-49 0.0 3.630445350361574E-5 0.0 0.6771506667494408 3.630445350361574E-5 50-51 0.0 3.630445350361574E-5 0.0 0.8221869584963857 3.630445350361574E-5 52-53 0.0 3.630445350361574E-5 0.0 0.990240273764623 3.630445350361574E-5 54-55 0.0 3.630445350361574E-5 0.0 1.1805482190305767 3.630445350361574E-5 56-57 0.0 3.630445350361574E-5 0.0 1.3684600703652918 5.4456680255423614E-5 58-59 0.0 3.630445350361574E-5 0.0 1.5818031913792896 9.076113375903935E-5 60-61 0.0 3.630445350361574E-5 0.0 1.797941755313066 1.0891336051084723E-4 62-63 0.0 3.630445350361574E-5 0.0 2.049259334691846 1.4521781401446296E-4 64-65 0.0 3.630445350361574E-5 0.0 2.29378798126545 1.4521781401446296E-4 66-67 0.0 3.630445350361574E-5 0.0 2.557666901556481 1.4521781401446296E-4 68-69 0.0 3.630445350361574E-5 0.0 2.9093299904192547 1.4521781401446296E-4 70-71 0.0 3.630445350361574E-5 0.0 3.352335084297126 1.4521781401446296E-4 72-73 0.0 3.630445350361574E-5 0.0 3.7088629699293842 1.4521781401446296E-4 74-75 0.0 3.630445350361574E-5 0.0 4.073160008611416 1.4521781401446296E-4 76-77 0.0 3.630445350361574E-5 0.0 4.465828977706524 1.6337004076627084E-4 78-79 0.0 3.630445350361574E-5 0.0 4.899921328249258 1.8152226751807872E-4 80-81 0.0 3.630445350361574E-5 0.0 5.299469991283301 1.9967449426988658E-4 82-83 0.0 3.630445350361574E-5 0.0 5.767198417997134 2.1782672102169446E-4 84-85 0.0 3.630445350361574E-5 0.0 6.2377767443110015 2.1782672102169446E-4 86-87 0.0 3.630445350361574E-5 0.0 6.720099561333289 2.1782672102169446E-4 88 0.0 3.630445350361574E-5 0.0 7.107213949042343 2.1782672102169446E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCA 1050 0.0 75.63291 4 TATAACG 1050 0.0 73.88704 2 TACCTGG 9765 0.0 70.97393 2 ACCTGGG 9740 0.0 69.04144 3 CCTGGGG 3655 0.0 62.997326 4 TACCCGG 600 0.0 62.692036 2 ATAACGC 1290 0.0 61.19962 3 TATCACG 385 0.0 61.063667 2 GTACCTG 11585 0.0 60.270397 1 GTATAAC 1550 0.0 53.692696 1 CCTGGGA 7170 0.0 51.840683 4 CTGGGGG 2705 0.0 51.768124 5 GTACCCG 810 0.0 50.501915 1 TCACGCA 550 0.0 46.136482 4 ATCACGC 520 0.0 45.18516 3 ACCCGGG 800 0.0 43.46812 3 ACAACGC 385 0.0 42.720512 3 CTGGGAA 7020 0.0 39.292927 5 GTACCGG 140 1.2732926E-11 36.94352 1 GTATCAC 765 0.0 36.877666 1 >>END_MODULE