FastQCFastQC Report
Mon 6 Jun 2016
ERR1211541_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211541_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4135655
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155710.37650626079786637No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132800.321109957189369No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86730.20971285080597876No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61580.1489002346665764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70600.049.218761
TACCTGG45750.043.884482
GTACCTG52400.038.638141
GTATCAA116700.037.6031381
TATAACG4000.034.0888372
ACCTGGG57850.033.873493
ATCAACG145600.029.7575233
TCAACGC145950.029.6861614
CAACGCA147150.029.4114275
TATCAAC150200.029.0191022
ATAACGC4250.028.7482643
AACGCAG152750.028.4807856
GTACATG126850.027.4675731
TACATGG129500.026.4686582
TAACGCA4650.026.2752934
ACATGGG130150.025.4549433
ACGCAGA171950.025.2198647
CGCAGAG176300.024.437998
CCTGGGG56400.024.2460584
CATGGGG86850.023.5367584
Produced by FastQC (version 0.11.5)