FastQCFastQC Report
Mon 6 Jun 2016
ERR1211541_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211541_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4135655
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164920.3987760100878821No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151410.3661088751358612No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105300.2546150488858476No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62080.1501092329993677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78950.047.232851
TACCTGG48150.044.5334552
GTACCTG54650.040.441831
GTATCAA127800.036.0593831
ACCTGGG59750.035.628253
TATAACG4550.033.071692
TCAACGC160650.028.5783734
CAACGCA163150.028.0821725
ATCAACG162700.028.0456733
TATCAAC165150.027.7330862
TAACGCA4950.027.530654
AACGCAG169750.027.1007186
CCTGGGG57200.025.6319854
ACGCAGA189900.024.1753227
GTACATG128550.023.8871421
CGCAGAG194800.023.5430938
ATAACGC5450.023.280993
TACATGG130350.023.1601732
ACATGGG128200.022.2869033
CATGGGG81350.021.8352364
Produced by FastQC (version 0.11.5)