##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211541_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4135655 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.118742013054764 33.0 31.0 34.0 30.0 34.0 2 32.33420389273283 34.0 31.0 34.0 30.0 34.0 3 32.347017582462755 34.0 31.0 34.0 30.0 34.0 4 35.988933796460294 37.0 35.0 37.0 35.0 37.0 5 35.914057386314866 37.0 35.0 37.0 35.0 37.0 6 35.9317588145046 37.0 35.0 37.0 35.0 37.0 7 35.858244703680555 37.0 35.0 37.0 35.0 37.0 8 35.85237018078152 37.0 35.0 37.0 35.0 37.0 9 37.50002502626549 39.0 37.0 39.0 35.0 39.0 10-11 37.46244258769167 39.0 37.0 39.0 34.5 39.0 12-13 37.37492307748108 39.0 37.0 39.0 34.0 39.0 14-15 38.6127925322591 40.0 38.0 41.0 34.0 41.0 16-17 38.53872167770281 40.0 38.0 41.0 34.0 41.0 18-19 38.521556802973166 40.0 38.0 41.0 34.0 41.0 20-21 38.452343099218865 40.0 38.0 41.0 34.0 41.0 22-23 38.335699302770664 40.0 38.0 41.0 34.0 41.0 24-25 38.22044500810634 40.0 38.0 41.0 33.5 41.0 26-27 38.04124751701967 40.0 37.0 41.0 33.0 41.0 28-29 38.07037990354611 40.0 37.5 41.0 33.0 41.0 30-31 37.98483928180663 40.0 37.0 41.0 33.0 41.0 32-33 37.785216247486794 40.0 37.0 41.0 33.0 41.0 34-35 37.653249968868295 40.0 37.0 41.0 33.0 41.0 36-37 37.57780726390378 40.0 37.0 41.0 32.5 41.0 38-39 37.472135369125326 40.0 36.5 41.0 32.0 41.0 40-41 37.299986217419004 40.0 36.0 41.0 31.0 41.0 42-43 37.14144494644742 40.0 36.0 41.0 31.0 41.0 44-45 36.952707733116036 39.0 36.0 41.0 30.5 41.0 46-47 36.686553278743034 39.0 35.0 41.0 30.0 41.0 48-49 36.45520044587859 39.0 35.0 41.0 29.5 41.0 50-51 36.24718394063335 39.0 35.0 40.0 29.0 41.0 52-53 36.0382788216135 39.0 35.0 40.0 28.0 41.0 54-55 35.836585014949264 38.5 35.0 40.0 28.0 41.0 56-57 35.6274465592512 38.0 34.0 40.0 27.0 41.0 58-59 35.35890143157492 38.0 34.0 40.0 26.5 41.0 60-61 35.0356122548907 38.0 34.0 40.0 26.0 41.0 62-63 34.70300822965165 37.0 33.0 40.0 26.0 41.0 64-65 34.378026455301516 36.5 33.0 39.5 26.0 41.0 66-67 34.031848763980555 36.0 33.0 39.0 25.0 40.5 68-69 33.63248711510027 35.5 32.0 39.0 24.5 40.0 70-71 33.2385879624872 35.0 32.0 38.0 24.0 40.0 72-73 32.82941311593932 35.0 31.0 37.5 23.0 40.0 74-75 32.42380674887049 35.0 31.0 37.0 22.0 39.0 76-77 30.894045683211004 33.5 29.5 35.5 20.5 37.5 78-79 31.73109749241656 34.0 30.5 36.0 22.0 38.0 80-81 31.70551641759286 34.0 31.0 36.0 21.5 37.5 82-83 31.42903723835765 34.0 30.5 35.0 20.5 37.0 84-85 31.13721502398048 34.0 30.0 35.0 20.0 36.5 86-87 30.842277583599213 34.0 30.0 35.0 20.0 36.0 88-89 30.578383351609357 34.0 29.5 35.0 19.0 36.0 90-91 30.29995974035552 34.0 29.0 35.0 18.5 35.0 92-93 30.09508094848337 34.0 29.0 35.0 18.0 35.0 94-95 29.87722609840521 34.0 29.0 35.0 18.0 35.0 96-97 29.674628807286872 34.0 29.0 35.0 17.5 35.0 98-99 29.50843203797222 33.5 29.0 35.0 17.0 35.0 100 27.627611103924288 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34124774195188223 1101 2 0.2725428618365413 1101 3 0.30540189399921047 1101 4 0.17164406029081647 1101 5 0.24376476953792547 1101 6 0.25893073061956073 1101 7 0.20764517387359405 1101 8 0.2083074848320976 1101 9 0.2611311303762065 1101 10-11 0.22534915561140423 1101 12-13 0.19853030042176556 1101 14-15 0.23459813636109317 1101 16-17 0.33811226501763514 1101 18-19 0.2954546416493713 1101 20-21 0.266923990112943 1101 22-23 0.21273820748573513 1101 24-25 0.27773205954176206 1101 26-27 0.19228799157286858 1101 28-29 0.37157188653520024 1101 30-31 0.33418709469538754 1101 32-33 0.42366581179039997 1101 34-35 0.3549311951823384 1101 36-37 0.4562201899680076 1101 38-39 0.4108722644643521 1101 40-41 0.3743210874187426 1101 42-43 0.4179384848983503 1101 44-45 0.3905560050481043 1101 46-47 0.4596332711214828 1101 48-49 0.4178304034256115 1101 50-51 0.36955283068217426 1101 52-53 0.4321853879167463 1101 54-55 0.35151824592816183 1101 56-57 0.3939886946399511 1101 58-59 0.43358841713212115 1101 60-61 0.5150333627630701 1101 62-63 0.4223054726625719 1101 64-65 0.3202936408532011 1101 66-67 0.21500058232408747 1101 68-69 0.29562762050772307 1101 70-71 0.29751774704110545 1101 72-73 0.10969074106919408 1101 74-75 0.15865850891291444 1101 76-77 0.22264099237484203 1101 78-79 0.36340964553916777 1101 80-81 0.3194955528550487 1101 82-83 0.1332700138688523 1101 84-85 0.21966315161654038 1101 86-87 0.1962274517664504 1101 88-89 0.15601902501909848 1101 90-91 0.1469511973715676 1101 92-93 -0.016423681973549265 1101 94-95 0.03484898819747073 1101 96-97 -0.05044382112721735 1101 98-99 -0.01786064954952593 1101 100 0.12140456531209765 1102 1 0.26548207507466515 1102 2 0.18242012386575368 1102 3 0.3041331025499403 1102 4 0.19678029000549913 1102 5 0.20163413350742587 1102 6 0.1521871733122353 1102 7 0.1913429808913989 1102 8 0.1567558713334236 1102 9 0.1862790204596081 1102 10-11 0.18497808277807337 1102 12-13 0.15683499127963074 1102 14-15 0.3173077997657643 1102 16-17 0.2342204862167634 1102 18-19 0.30558332795774845 1102 20-21 0.32936260127392814 1102 22-23 0.3011900456111505 1102 24-25 0.2886626141046307 1102 26-27 0.2254236555078748 1102 28-29 0.3249894156720785 1102 30-31 0.29016017183376164 1102 32-33 0.3319141813241373 1102 34-35 0.310896597202607 1102 36-37 0.32607083943312176 1102 38-39 0.37680854074304193 1102 40-41 0.31725682639073227 1102 42-43 0.3068433293166848 1102 44-45 0.27561440470704923 1102 46-47 0.35991799137044467 1102 48-49 0.49448662612083893 1102 50-51 0.3723768383354056 1102 52-53 0.2964828836298139 1102 54-55 0.3786637101900041 1102 56-57 0.3121125442196515 1102 58-59 0.4223832047148832 1102 60-61 0.3907377061729349 1102 62-63 0.28487814900967123 1102 64-65 0.3280486092452648 1102 66-67 0.33400173906365893 1102 68-69 0.4227527323901157 1102 70-71 0.38385868556907354 1102 72-73 0.33853010147666396 1102 74-75 0.41880508768907276 1102 76-77 0.370376483883593 1102 78-79 0.4039448496837501 1102 80-81 0.3286147795440435 1102 82-83 0.2886007247813893 1102 84-85 0.24398436610786334 1102 86-87 0.17311181189984381 1102 88-89 0.1959478464095561 1102 90-91 0.17642960699799204 1102 92-93 0.16780738353428504 1102 94-95 0.22548228858348196 1102 96-97 0.1870815697130368 1102 98-99 0.21577270584553787 1102 100 0.14172634112814464 1103 1 0.24977520345991877 1103 2 0.22568893930305478 1103 3 0.2849443686024671 1103 4 0.15447519232252915 1103 5 0.1470057350105165 1103 6 0.17179297435558993 1103 7 0.16058051504866455 1103 8 0.1623129904239775 1103 9 0.2076459101123902 1103 10-11 0.20504040191300987 1103 12-13 0.2307436001204124 1103 14-15 0.30263764464559983 1103 16-17 0.29926917892019134 1103 18-19 0.2712817910391081 1103 20-21 0.28332588093983446 1103 22-23 0.2409642131375449 1103 24-25 0.27706291750295264 1103 26-27 0.25536067405694496 1103 28-29 0.3371763657818292 1103 30-31 0.4249632163371686 1103 32-33 0.353164013825392 1103 34-35 0.43195509717400427 1103 36-37 0.5009444810614241 1103 38-39 0.5093335542101372 1103 40-41 0.5082712045988913 1103 42-43 0.49174865844549487 1103 44-45 0.5781472930152347 1103 46-47 0.6166849409964286 1103 48-49 0.5298594831446692 1103 50-51 0.525952077623451 1103 52-53 0.5062360048658903 1103 54-55 0.49825321250081345 1103 56-57 0.5982752370543878 1103 58-59 0.5217997619234325 1103 60-61 0.6175384089573939 1103 62-63 0.6008512524534382 1103 64-65 0.6240928166296484 1103 66-67 0.5708411844536911 1103 68-69 0.5155161815321208 1103 70-71 0.5795319288095229 1103 72-73 0.5280697227547861 1103 74-75 0.5918773258218053 1103 76-77 0.5406876322550431 1103 78-79 0.5367585465319316 1103 80-81 0.4839473159224674 1103 82-83 0.44160488450973645 1103 84-85 0.42511521402206753 1103 86-87 0.48921992221826827 1103 88-89 0.4928892519861421 1103 90-91 0.5972560660033217 1103 92-93 0.5821058066079132 1103 94-95 0.6232917792452106 1103 96-97 0.5567776185086402 1103 98-99 0.465033887563159 1103 100 0.6065469955577711 1104 1 0.23575150880881068 1104 2 0.1768824946618821 1104 3 0.21356919292625065 1104 4 0.07647960847317137 1104 5 0.11851985936427667 1104 6 0.16371300912162212 1104 7 0.1830965886134308 1104 8 0.209778601743686 1104 9 0.1954460914477707 1104 10-11 0.20325661264792672 1104 12-13 0.21466833597406065 1104 14-15 0.32027149541889344 1104 16-17 0.25235716941335085 1104 18-19 0.3032043231939241 1104 20-21 0.20505837316901676 1104 22-23 0.2635283814563323 1104 24-25 0.23494220237073904 1104 26-27 0.33361414449151994 1104 28-29 0.2773310576927912 1104 30-31 0.23932619550036804 1104 32-33 0.2529198572914453 1104 34-35 0.3392236857498716 1104 36-37 0.2413358662061711 1104 38-39 0.2742659743297722 1104 40-41 0.3308351308143571 1104 42-43 0.47242914008582915 1104 44-45 0.4190774092425187 1104 46-47 0.4364944299004989 1104 48-49 0.4625967348456115 1104 50-51 0.4440220821684804 1104 52-53 0.4981486834159199 1104 54-55 0.4977326671016513 1104 56-57 0.5269200814597141 1104 58-59 0.4624297212584523 1104 60-61 0.44553334099014563 1104 62-63 0.36857947695993687 1104 64-65 0.49026979210188415 1104 66-67 0.5384817023797197 1104 68-69 0.5739474666062634 1104 70-71 0.46838849069852273 1104 72-73 0.5981613784127333 1104 74-75 0.6030772345302395 1104 76-77 0.5027732759290657 1104 78-79 0.6341031900008183 1104 80-81 0.7265420041571851 1104 82-83 0.565414376393548 1104 84-85 0.6124582018767661 1104 86-87 0.6024950101506548 1104 88-89 0.5840446406890649 1104 90-91 0.43223570185573124 1104 92-93 0.45409817346296677 1104 94-95 0.48662301652256446 1104 96-97 0.5146682868879111 1104 98-99 0.465936630286393 1104 100 0.6111024487160392 1105 1 0.2354005684877123 1105 2 0.20666176350093934 1105 3 0.22778135871627114 1105 4 0.1084593450915392 1105 5 0.08396373819320502 1105 6 0.09502564868813579 1105 7 0.18761690314740065 1105 8 0.14237929247664738 1105 9 0.21819498285312022 1105 10-11 0.1851636423903642 1105 12-13 0.20879371917563816 1105 14-15 0.2750626351282719 1105 16-17 0.2802484583464562 1105 18-19 0.2735666581394298 1105 20-21 0.2672019736586648 1105 22-23 0.3392355360810342 1105 24-25 0.2944403450116866 1105 26-27 0.2860337912228843 1105 28-29 0.2879938861701845 1105 30-31 0.4213767340110266 1105 32-33 0.4333321098640539 1105 34-35 0.4777725820940688 1105 36-37 0.4609607255372339 1105 38-39 0.4721302759945232 1105 40-41 0.5238308587121452 1105 42-43 0.5585600830174542 1105 44-45 0.5064121607303349 1105 46-47 0.5168618095249329 1105 48-49 0.47099425554466734 1105 50-51 0.5511214623432394 1105 52-53 0.529194201941884 1105 54-55 0.40160352332124205 1105 56-57 0.49084448888632437 1105 58-59 0.5420528872707209 1105 60-61 0.5358374681019384 1105 62-63 0.5213648805518929 1105 64-65 0.6098340447309383 1105 66-67 0.6009972685655569 1105 68-69 0.5440458554839722 1105 70-71 0.591883641809595 1105 72-73 0.5424990261812539 1105 74-75 0.6086890341400633 1105 76-77 0.6290140729177836 1105 78-79 0.6211262297197244 1105 80-81 0.6228031562489669 1105 82-83 0.5897394653809798 1105 84-85 0.6216538401697598 1105 86-87 0.6971429938665707 1105 88-89 0.6447217565438166 1105 90-91 0.6261123083037496 1105 92-93 0.7246589794827116 1105 94-95 0.7726354976932122 1105 96-97 0.7680742918197012 1105 98-99 0.7040925355593188 1105 100 0.7995441368137719 1106 1 0.32838063365509385 1106 2 0.22224112184170508 1106 3 0.27082985689114736 1106 4 0.16805048871377437 1106 5 0.15734554793057498 1106 6 0.11437774848566562 1106 7 0.20164516169789692 1106 8 0.15673034001690667 1106 9 0.22061648083205654 1106 10-11 0.2484984271130486 1106 12-13 0.232788595771261 1106 14-15 0.32397692333065464 1106 16-17 0.30034497573772967 1106 18-19 0.30642321007377404 1106 20-21 0.39338561795216265 1106 22-23 0.34783458251823873 1106 24-25 0.37664181236630867 1106 26-27 0.393718680082749 1106 28-29 0.33629561550058895 1106 30-31 0.4007812289638153 1106 32-33 0.43082490734444434 1106 34-35 0.46891816370206385 1106 36-37 0.4489102026742273 1106 38-39 0.41556056213592996 1106 40-41 0.47017669576466403 1106 42-43 0.42725898504141924 1106 44-45 0.5439291964127477 1106 46-47 0.5297120479156376 1106 48-49 0.5241514627812904 1106 50-51 0.4386327650377453 1106 52-53 0.4969399040151927 1106 54-55 0.3702149065678597 1106 56-57 0.44649206252618967 1106 58-59 0.5189385685891708 1106 60-61 0.5121288054846502 1106 62-63 0.5725525524508157 1106 64-65 0.5893390483956935 1106 66-67 0.5173613205543219 1106 68-69 0.4690209278720161 1106 70-71 0.5241338758461254 1106 72-73 0.5577381062906923 1106 74-75 0.6076605796520127 1106 76-77 0.511573452884253 1106 78-79 0.6720219546524255 1106 80-81 0.7111265838500351 1106 82-83 0.61825258956911 1106 84-85 0.7020951350993307 1106 86-87 0.4786489554482962 1106 88-89 0.5684376761078198 1106 90-91 0.5750060593469328 1106 92-93 0.7025356694441811 1106 94-95 0.8585363387733373 1106 96-97 0.7370869116500316 1106 98-99 0.7709454880480884 1106 100 0.9512252085196629 1107 1 0.20591683021242346 1107 2 0.1417026927285363 1107 3 0.17011061483631806 1107 4 0.13717795937073163 1107 5 0.08784948921492486 1107 6 0.1119058433889677 1107 7 0.17550117506151253 1107 8 0.10408858016066347 1107 9 0.15894405901507724 1107 10-11 0.12457978158116134 1107 12-13 0.14401361558976333 1107 14-15 0.1774103075881115 1107 16-17 0.13308174696820885 1107 18-19 0.09848744612676086 1107 20-21 0.17636799381996582 1107 22-23 0.250600931246872 1107 24-25 0.26930967842235276 1107 26-27 0.21763942053242147 1107 28-29 0.23476694224334693 1107 30-31 0.12269009455800273 1107 32-33 0.1338668846022486 1107 34-35 0.14407174369140563 1107 36-37 0.2334975400904966 1107 38-39 0.2122163880925072 1107 40-41 0.1781477502659996 1107 42-43 0.14170690861568147 1107 44-45 0.2235013398014516 1107 46-47 0.22369096051233583 1107 48-49 0.22275642374240334 1107 50-51 0.18117427035169698 1107 52-53 0.19626854160009088 1107 54-55 0.14734120407084816 1107 56-57 0.08873291776952641 1107 58-59 0.26020540453301777 1107 60-61 0.18968131107484965 1107 62-63 0.23430277011669176 1107 64-65 0.2566674251926955 1107 66-67 0.26776689272237775 1107 68-69 0.24691363244808429 1107 70-71 0.3892254775948132 1107 72-73 0.2800901351010978 1107 74-75 0.28179685190047365 1107 76-77 0.2970458930660307 1107 78-79 0.3302255450282523 1107 80-81 0.3267491591540086 1107 82-83 0.321332696288529 1107 84-85 0.2856124589926132 1107 86-87 0.25933108221666856 1107 88-89 0.3187635630852448 1107 90-91 0.25789262698859616 1107 92-93 0.27724200406441923 1107 94-95 0.4717877901891576 1107 96-97 0.4899329162012904 1107 98-99 0.40526383410461975 1107 100 0.6926423597094882 1108 1 0.2467453809609026 1108 2 0.2249548592319286 1108 3 0.2650219357505321 1108 4 0.15040180019141047 1108 5 0.1656433200578391 1108 6 0.1933958626048451 1108 7 0.2092215115884386 1108 8 0.2028146791865808 1108 9 0.2096279170741937 1108 10-11 0.21019746901898628 1108 12-13 0.2293287906987942 1108 14-15 0.317811171095002 1108 16-17 0.34887662653310514 1108 18-19 0.3130070850403399 1108 20-21 0.3314120484455856 1108 22-23 0.3625851649439369 1108 24-25 0.3448316242224081 1108 26-27 0.3821908437602346 1108 28-29 0.36956394529102 1108 30-31 0.38735446578520794 1108 32-33 0.3568322450773138 1108 34-35 0.395462696605712 1108 36-37 0.4415333770227221 1108 38-39 0.5124675398375658 1108 40-41 0.5402848675522947 1108 42-43 0.5302184970124912 1108 44-45 0.5538635772058456 1108 46-47 0.5872712026505056 1108 48-49 0.5585786006462143 1108 50-51 0.5289888227938278 1108 52-53 0.5410363661001156 1108 54-55 0.5367075442839635 1108 56-57 0.5879828400305129 1108 58-59 0.7367535997047483 1108 60-61 0.6409530987552543 1108 62-63 0.6453374462468915 1108 64-65 0.6711978569989796 1108 66-67 0.7093290865778243 1108 68-69 0.657440174661879 1108 70-71 0.6652664445474272 1108 72-73 0.7072100894237607 1108 74-75 0.6620823975000718 1108 76-77 0.6667080340136593 1108 78-79 0.76499486770253 1108 80-81 0.8445896032173899 1108 82-83 0.8167808126645895 1108 84-85 0.7694536872388973 1108 86-87 0.8076047005377163 1108 88-89 0.8071600713202969 1108 90-91 0.7450796519692382 1108 92-93 0.7035887664985125 1108 94-95 0.8469600141892322 1108 96-97 0.8831426677685243 1108 98-99 0.9569995274838234 1108 100 1.0731866422689293 1109 1 0.11302443326105305 1109 2 0.10290440376877541 1109 3 0.15046438996386513 1109 4 0.09456099598047985 1109 5 0.07818673326747216 1109 6 0.06394713525782691 1109 7 0.09811298757747267 1109 8 0.11947258309467657 1109 9 0.09061263081740378 1109 10-11 0.10026102655876912 1109 12-13 0.1789014935541431 1109 14-15 0.19291782638510568 1109 16-17 0.2660430208031599 1109 18-19 0.21930703276423458 1109 20-21 0.2347590717114585 1109 22-23 0.24869534136080773 1109 24-25 0.21526121971344736 1109 26-27 0.3007944556191049 1109 28-29 0.3169333150934506 1109 30-31 0.29087086065613477 1109 32-33 0.3035169930703958 1109 34-35 0.33864509517803754 1109 36-37 0.357553602470972 1109 38-39 0.3822429328097243 1109 40-41 0.3313437743205512 1109 42-43 0.4121330646229353 1109 44-45 0.4185251494550357 1109 46-47 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78-79 -0.16311985673295126 2316 80-81 -0.08829243720164826 2316 82-83 -0.08465020105591847 2316 84-85 -0.1685903132353026 2316 86-87 -0.1009745574066443 2316 88-89 0.007008297137815589 2316 90-91 0.06810167404434964 2316 92-93 0.08583489181126325 2316 94-95 0.20911477861947603 2316 96-97 0.15570620858376927 2316 98-99 0.01043168302038211 2316 100 -0.17284624889665778 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 5.0 10 7.0 11 26.0 12 45.0 13 161.0 14 447.0 15 1300.0 16 2902.0 17 6085.0 18 10933.0 19 16096.0 20 22070.0 21 28796.0 22 36196.0 23 41145.0 24 44098.0 25 47936.0 26 54074.0 27 64687.0 28 78207.0 29 93826.0 30 113241.0 31 137097.0 32 167644.0 33 210725.0 34 278970.0 35 380770.0 36 549182.0 37 836600.0 38 793242.0 39 119137.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.43786429811382 19.20962316477174 13.240411546695082 24.112100990419343 2 15.558132703719851 21.234284060658528 39.30089385047712 23.906689385144496 3 17.520784615455295 26.623361707076864 30.780042997066225 25.07581068040161 4 12.252352850429753 17.165809641209957 37.32988653218307 33.25195097617723 5 12.51963140473446 37.571322903390154 35.13904777380172 14.76999791807366 6 30.770925237956604 37.022623891703994 18.32585039079917 13.880600479540227 7 26.890202398410896 32.852450216471155 22.54634392859172 17.711003456526235 8 26.047110734115194 35.66613260702512 20.447028714934504 17.839727943925187 9 26.67735260251462 15.140531582187485 20.312144101029727 37.86997171426817 10-11 24.802238097713662 26.017087982435672 29.07214213951599 20.108531780334673 12-13 25.1265398105016 23.97188595276927 28.996785273433108 21.90478896329602 14-15 22.513930683289587 25.349382383201696 26.7536823066721 25.38300462683662 16-17 22.259463648503157 28.00697113074134 27.30462305901583 22.428942161739663 18-19 22.006486107967678 27.11943443382448 29.289550653737006 21.58452880447083 20-21 22.76533366251295 26.489798716417116 29.229735413822667 21.515132207247266 22-23 22.496753536834373 26.305376815205427 29.12466454825953 22.07320509970066 24-25 22.192487572703133 26.341130084866865 29.22966960001973 22.23671274241027 26-27 22.188179458213078 26.504963671956606 29.112076269547117 22.194780600283195 28-29 22.228163826927545 26.796897829479278 29.1116532573528 21.863285086240385 30-31 22.591947112841616 26.545293636530147 29.195200935294114 21.66755831533412 32-33 22.379328884669107 26.633746406311037 29.083530274158313 21.903394434861543 34-35 22.527362517600682 26.755866833772902 28.997256191704352 21.719514456922067 36-37 22.530298760290663 26.75664379577699 28.92712350655218 21.785933937380165 38-39 22.500842381096714 26.899262585572558 28.754145258539964 21.84574977479076 40-41 22.55383841215963 26.73780517662694 28.895537091639405 21.81281931957402 42-43 22.3553115271502 26.84308562988556 28.68478082662822 22.11682201633602 44-45 22.30590374044739 27.071764421540607 28.58279543397225 22.03953640403975 46-47 22.265691263141925 26.83687663716718 28.493869672273362 22.403562427417537 48-49 22.297836407117657 26.992659940142488 28.446162758122373 22.26334089461748 50-51 22.3716977098705 27.297638001679196 28.22408563126555 22.106578657184752 52-53 22.412907166507757 27.323666467496928 27.986379401189843 22.277046964805475 54-55 22.312019223503455 27.18781302422585 28.24803155556227 22.252136196708427 56-57 22.431043029343133 27.252505712662163 27.978430922126442 22.338020335868265 58-59 22.374825611431497 27.267667999711282 28.090805014749655 22.266701374107566 60-61 22.59118606540995 27.280958961267963 28.14676244414166 21.981092529180422 62-63 22.44263075684524 27.375862101487776 28.035096620419488 22.146410521247496 64-65 22.392121188819626 27.093805170212914 28.027296356399244 22.486777284568216 66-67 22.21512742047639 27.173938408549585 28.048029806066864 22.562904364907162 68-69 22.097890192505062 27.222390695991745 28.119041953354206 22.560677158148987 70-71 22.156524468456958 27.10787603678596 28.04876824973615 22.68683124502093 72-73 22.07971796368582 27.436417777051254 27.765639894432255 22.71822436483068 74-75 22.19096881804945 27.37220289589086 27.697951626500906 22.738876659558784 76-77 22.045300503755374 27.250716291153342 27.847627600658587 22.8563556044327 78-79 21.9926022939853 27.21746189426929 27.942844305758953 22.847091505986455 80-81 22.055633016412834 27.390372735841712 27.626781498961893 22.927212748783557 82-83 22.11873408096952 27.434626170620085 27.666077870117157 22.780561878293238 84-85 22.052222134218635 27.509276149006062 27.588237534711872 22.85026418206343 86-87 22.007391725538504 27.44177199224574 27.62304013644328 22.92779614577248 88-89 21.74707042799213 27.338596925282584 27.739835770320298 23.17449687640499 90-91 22.10337018975774 27.285218031907082 27.64798982657237 22.963421951762804 92-93 21.9105120722678 27.390292818149288 27.539810649447748 23.15938446013517 94-95 21.78896644259262 27.237254223959635 27.621168641631378 23.35261069181637 96-97 21.658762098705118 27.223876229218767 27.655148812259057 23.46221285981706 98-99 21.680854076553125 27.102037983330128 27.549661779097214 23.667446161019534 100 20.83115271246664 27.677084955699133 27.810364825529373 23.681397506304855 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2358.0 1 1816.0 2 2969.0 3 4288.5 4 2752.5 5 1826.5 6 2656.0 7 4700.5 8 7066.0 9 7992.0 10 6675.0 11 5094.0 12 4955.5 13 5338.0 14 5748.0 15 6009.5 16 6338.0 17 6717.0 18 7117.5 19 7565.5 20 8165.5 21 9203.0 22 10757.0 23 12913.5 24 15974.5 25 19696.0 26 23937.0 27 28775.5 28 34121.5 29 40291.0 30 47024.0 31 54006.0 32 61324.0 33 68595.0 34 75556.0 35 82451.0 36 90526.5 37 98957.0 38 106132.5 39 112213.0 40 118003.5 41 123620.5 42 129060.5 43 134931.0 44 139939.5 45 145260.0 46 150899.0 47 155378.5 48 161555.0 49 166796.5 50 168818.0 51 165773.5 52 158204.0 53 150632.5 54 142303.5 55 130980.0 56 117812.5 57 105631.5 58 93209.5 59 79388.0 60 65304.0 61 53209.0 62 43324.0 63 33756.0 64 25182.0 65 18781.0 66 14000.0 67 10611.5 68 8022.0 69 6077.0 70 4729.0 71 3705.5 72 2892.0 73 2292.0 74 1799.0 75 1335.5 76 977.0 77 642.5 78 403.5 79 278.5 80 209.5 81 149.0 82 93.0 83 70.0 84 60.0 85 40.5 86 22.5 87 14.5 88 13.0 89 10.5 90 6.5 91 5.5 92 5.5 93 4.0 94 1.0 95 1.0 96 0.5 97 1.0 98 1.5 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003240115531880682 2 0.06647072833686561 3 5.803191997398236E-4 4 7.979388996422574E-4 5 0.0014991579326612107 6 6.528590997073015E-4 7 0.0 8 8.946587662655614E-4 9 2.4179966655825985E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.649490830352145E-4 18-19 0.001136458432823821 20-21 0.0029378659486828567 22-23 1.2089983327912993E-5 24-25 2.4179966655825985E-5 26-27 1.6925976659078187E-4 28-29 8.342088496259964E-4 30-31 0.001390348082709994 32-33 0.0025751664488454667 34-35 0.003917154598243809 36-37 0.0011727283828075602 38-39 0.0013661681160541679 40-41 0.004872263281148935 42-43 0.006117531563923973 44-45 0.004848083314493109 46-47 0.0065890409137125796 48-49 0.00808819884637379 50-51 0.008293728562948311 52-53 0.0037237148649972014 54-55 0.0022366469156639033 56-57 0.002538896498861728 58-59 0.0032522055152085947 60-61 0.007386979813354837 62-63 0.013444061460639246 64-65 0.003820434731620505 66-67 0.00941809701244422 68-69 0.011872363628010557 70-71 0.0052833227142979776 72-73 0.00727816996340362 74-75 0.002550986482189641 76-77 0.002804876132075814 78-79 0.0077134093632084885 80-81 0.005633932230807453 82-83 0.01612803775943593 84-85 0.017470025908834272 86-87 0.021677340106947992 88-89 0.020214452124270522 90-91 0.023261127922904596 92-93 0.033017744468530376 94-95 0.03700743896674166 96-97 0.022124669490080774 98-99 0.017155686342308536 100 0.01806243509190201 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4135655.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.93840164332798 #Duplication Level Percentage of deduplicated Percentage of total 1 77.35993944362582 51.783506975809544 2 15.038553648826806 20.133134885598082 3 3.963200753601555 7.958709715131629 4 1.4697605752048404 3.9353369481036133 5 0.6943010649868323 2.323770177473947 6 0.4044503271504188 1.6243955066142075 7 0.24539004763259906 1.1498212297394406 8 0.16712269040372527 0.8949540619166492 9 0.11604379909133139 0.6991007788613878 >10 0.5154417574213648 5.775781433845165 >50 0.01698214942339351 0.776839029269559 >100 0.007885093569461233 0.9574905926741137 >500 5.909584974278187E-4 0.29012833273882055 >1k 1.6884528219042878E-4 0.330279513043028 >5k 4.2211320547607195E-5 0.17541066104637845 >10k+ 1.2663396164282157E-4 1.191340158134416 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16492 0.3987760100878821 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15141 0.3661088751358612 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10530 0.2546150488858476 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6208 0.1501092329993677 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.253989996747795E-5 2 0.0 0.0 0.0 4.8359933311651964E-5 7.253989996747795E-5 3 0.0 0.0 0.0 4.8359933311651964E-5 7.253989996747795E-5 4 0.0 0.0 0.0 4.8359933311651964E-5 7.253989996747795E-5 5 0.0 0.0 0.0 4.8359933311651964E-5 7.253989996747795E-5 6 2.4179966655825982E-5 0.0 0.0 4.8359933311651964E-5 1.2089983327912991E-4 7 2.4179966655825982E-5 0.0 0.0 4.8359933311651964E-5 1.2089983327912991E-4 8 2.4179966655825982E-5 0.0 0.0 4.8359933311651964E-5 1.2089983327912991E-4 9 2.4179966655825982E-5 0.0 0.0 7.253989996747795E-5 1.450797999349559E-4 10-11 2.4179966655825982E-5 0.0 0.0 7.253989996747795E-5 1.450797999349559E-4 12-13 2.4179966655825982E-5 0.0 0.0 7.253989996747795E-5 1.450797999349559E-4 14-15 2.4179966655825982E-5 0.0 0.0 7.253989996747795E-5 1.8134974991869488E-4 16-17 2.4179966655825982E-5 0.0 0.0 7.253989996747795E-5 1.9343973324660786E-4 18-19 2.4179966655825982E-5 0.0 0.0 9.671986662330393E-5 1.9343973324660786E-4 20-21 2.4179966655825982E-5 0.0 0.0 1.0880984995121692E-4 2.0552971657452083E-4 22-23 2.4179966655825982E-5 0.0 0.0 2.1761969990243384E-4 2.4179966655825982E-4 24-25 2.4179966655825982E-5 1.2089983327912991E-5 0.0 3.506095165094767E-4 2.659796332140858E-4 26-27 2.4179966655825982E-5 2.4179966655825982E-5 0.0 7.012190330189535E-4 2.901595998699118E-4 28-29 2.4179966655825982E-5 2.4179966655825982E-5 0.0 0.002091567115728947 2.901595998699118E-4 30-31 2.4179966655825982E-5 2.4179966655825982E-5 0.0 0.006250521380531017 2.901595998699118E-4 32-33 2.4179966655825982E-5 2.4179966655825982E-5 0.0 0.015825788176238108 2.901595998699118E-4 34-35 2.4179966655825982E-5 4.8359933311651964E-5 0.0 0.03006778853651961 2.901595998699118E-4 36-37 2.4179966655825982E-5 6.0449916639564955E-5 0.0 0.05296621695958681 2.901595998699118E-4 38-39 2.4179966655825982E-5 7.253989996747795E-5 0.0 0.09011873572626344 2.901595998699118E-4 40-41 2.4179966655825982E-5 7.253989996747795E-5 0.0 0.13563752295585585 2.901595998699118E-4 42-43 2.4179966655825982E-5 9.671986662330393E-5 0.0 0.17947580250286835 2.901595998699118E-4 44-45 2.4179966655825982E-5 9.671986662330393E-5 0.0 0.2319221501793549 2.901595998699118E-4 46-47 2.4179966655825982E-5 9.671986662330393E-5 0.0 0.2970629803501501 3.1433956652573774E-4 48-49 2.4179966655825982E-5 1.0880984995121692E-4 0.0 0.3677047529351457 3.3851953318156375E-4 50-51 2.4179966655825982E-5 1.2089983327912991E-4 0.0 0.4457939552501357 3.3851953318156375E-4 52-53 2.4179966655825982E-5 1.2089983327912991E-4 0.0 0.5243425769315864 3.7478948316530274E-4 54-55 2.4179966655825982E-5 1.450797999349559E-4 0.0 0.6163352600736764 4.4732938313278065E-4 56-57 2.4179966655825982E-5 1.6925976659078187E-4 0.0 0.7217236447430939 4.5941936646069363E-4 58-59 2.4179966655825982E-5 1.6925976659078187E-4 0.0 0.841208949972858 4.5941936646069363E-4 60-61 2.4179966655825982E-5 1.9343973324660786E-4 0.0 0.9696771128152615 4.5941936646069363E-4 62-63 4.8359933311651964E-5 1.9343973324660786E-4 0.0 1.1036462180718654 4.8359933311651964E-4 64-65 4.8359933311651964E-5 1.9343973324660786E-4 0.0 1.2476500094906369 4.8359933311651964E-4 66-67 4.8359933311651964E-5 1.9343973324660786E-4 0.0 1.4130409814164866 4.8359933311651964E-4 68-69 4.8359933311651964E-5 1.9343973324660786E-4 0.0 1.6071456637461297 4.8359933311651964E-4 70-71 4.8359933311651964E-5 1.9343973324660786E-4 0.0 1.8137997487701465 5.319592664281716E-4 72-73 4.8359933311651964E-5 1.9343973324660786E-4 0.0 2.018410626611746 5.803191997398236E-4 74-75 4.8359933311651964E-5 1.9343973324660786E-4 0.0 2.2335760598986134 5.803191997398236E-4 76-77 4.8359933311651964E-5 2.2970968323034684E-4 0.0 2.4717003715251877 5.924091830677365E-4 78-79 4.8359933311651964E-5 2.4179966655825982E-4 0.0 2.7492138488341027 6.286791330514755E-4 80-81 4.8359933311651964E-5 2.538896498861728E-4 0.0 3.037741300954746 6.407691163793886E-4 82-83 4.8359933311651964E-5 2.659796332140858E-4 0.0 3.345503916550099 6.649490830352145E-4 84-85 4.8359933311651964E-5 2.659796332140858E-4 0.0 3.6564582877440213 6.770390663631275E-4 86-87 4.8359933311651964E-5 2.659796332140858E-4 0.0 3.981340319731699 6.770390663631275E-4 88 4.8359933311651964E-5 2.659796332140858E-4 0.0 4.243753407864051 6.770390663631275E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7895 0.0 47.23285 1 TACCTGG 4815 0.0 44.533455 2 GTACCTG 5465 0.0 40.44183 1 GTATCAA 12780 0.0 36.059383 1 ACCTGGG 5975 0.0 35.62825 3 TATAACG 455 0.0 33.07169 2 TCAACGC 16065 0.0 28.578373 4 CAACGCA 16315 0.0 28.082172 5 ATCAACG 16270 0.0 28.045673 3 TATCAAC 16515 0.0 27.733086 2 TAACGCA 495 0.0 27.53065 4 AACGCAG 16975 0.0 27.100718 6 CCTGGGG 5720 0.0 25.631985 4 ACGCAGA 18990 0.0 24.175322 7 GTACATG 12855 0.0 23.887142 1 CGCAGAG 19480 0.0 23.543093 8 ATAACGC 545 0.0 23.28099 3 TACATGG 13035 0.0 23.160173 2 ACATGGG 12820 0.0 22.286903 3 CATGGGG 8135 0.0 21.835236 4 >>END_MODULE