FastQCFastQC Report
Mon 6 Jun 2016
ERR1211540_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211540_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5540654
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212410.3833662957477583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195510.35286448134101134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123700.22325884272867427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108500.050.5470281
GTATCAA174150.034.9521981
TACCTGG51850.031.7401082
GTACCTG58400.029.7449321
GTACATG199600.029.0569211
TCAACGC212400.028.0534
ATCAACG213650.027.9773543
CAACGCA213500.027.8636935
TACATGG204250.027.4872552
AACGCAG219900.027.153956
TATCAAC222350.026.8568922
ACATGGG205800.026.2816353
CATGGGG128500.025.8541854
ACCTGGG65300.024.971163
ACGCAGA249350.023.758687
ATGGGGG86350.023.127545
CGCAGAG255800.023.0312528
GAGTACT171100.021.10469412-13
GCAGAGT280650.020.4917959
CAGAGTA270200.019.9800410-11
Produced by FastQC (version 0.11.5)