FastQCFastQC Report
Mon 6 Jun 2016
ERR1211540_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211540_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5540654
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229730.41462614341195103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212620.3837453123764812No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146620.26462580049214407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112550.047.292051
GTATCAA180300.034.502561
TACCTGG48150.032.224432
GTACCTG54650.029.1673321
TCAACGC227800.026.859234
ATCAACG227900.026.8062023
CAACGCA229650.026.6837835
GTACATG205400.026.509121
AACGCAG235700.026.057736
TATCAAC236050.026.0338952
TACATGG212200.025.392582
CATGGGG124850.024.879934
ACATGGG211150.024.7930743
ACCTGGG61850.024.6173653
ATGGGGG79300.024.000385
ACGCAGA272500.022.4350747
CGCAGAG277300.021.9958888
TGGGGGG75700.020.733436
GAGTACT184750.020.11983512-13
GCAGAGT303400.019.8092379
Produced by FastQC (version 0.11.5)