##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211540_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5540654 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.118705120370265 33.0 31.0 34.0 30.0 34.0 2 32.33864359694722 34.0 31.0 34.0 30.0 34.0 3 32.346670627691246 34.0 31.0 34.0 30.0 34.0 4 35.98982881804206 37.0 35.0 37.0 35.0 37.0 5 35.91401953632189 37.0 35.0 37.0 35.0 37.0 6 35.93290394960595 37.0 35.0 37.0 35.0 37.0 7 35.85736358920806 37.0 35.0 37.0 35.0 37.0 8 35.85165993039811 37.0 35.0 37.0 35.0 37.0 9 37.49466922135907 39.0 37.0 39.0 35.0 39.0 10-11 37.45818020760726 39.0 37.0 39.0 34.5 39.0 12-13 37.36721062170639 39.0 37.0 39.0 34.0 39.0 14-15 38.595366539762274 40.0 38.0 41.0 34.0 41.0 16-17 38.519679896994106 40.0 38.0 41.0 34.0 41.0 18-19 38.5030759004262 40.0 38.0 41.0 34.0 41.0 20-21 38.44234507334333 40.0 38.0 41.0 34.0 41.0 22-23 38.333936210418486 40.0 38.0 41.0 34.0 41.0 24-25 38.2216399002717 40.0 38.0 41.0 33.5 41.0 26-27 38.03879442751705 40.0 37.0 41.0 33.0 41.0 28-29 38.07333087393654 40.0 37.5 41.0 33.0 41.0 30-31 37.98772662938346 40.0 37.0 41.0 33.0 41.0 32-33 37.79033332527171 40.0 37.0 41.0 33.0 41.0 34-35 37.668371639882224 40.0 37.0 41.0 33.0 41.0 36-37 37.607818228678425 40.0 37.0 41.0 32.5 41.0 38-39 37.50695342102214 40.0 36.5 41.0 32.0 41.0 40-41 37.33805251149052 40.0 36.0 41.0 31.0 41.0 42-43 37.180434385543656 40.0 36.0 41.0 31.0 41.0 44-45 36.986896492724505 39.0 36.0 41.0 30.5 41.0 46-47 36.70966171141529 39.0 35.0 41.0 30.0 41.0 48-49 36.471576730833576 39.0 35.0 41.0 29.5 41.0 50-51 36.26289874805393 39.0 35.0 40.5 29.0 41.0 52-53 36.058209464081315 39.0 35.0 40.0 28.0 41.0 54-55 35.85117262330404 38.5 35.0 40.0 28.0 41.0 56-57 35.64277709815484 38.0 34.0 40.0 27.5 41.0 58-59 35.370222540515975 38.0 34.0 40.0 27.0 41.0 60-61 35.04055477927335 37.5 34.0 40.0 26.0 41.0 62-63 34.70126198098636 37.0 33.0 40.0 26.0 41.0 64-65 34.378202464907574 36.5 33.0 39.5 26.0 41.0 66-67 34.0260290572196 36.0 33.0 39.0 25.0 40.5 68-69 33.61978585921445 35.5 32.0 39.0 24.5 40.0 70-71 33.22691238254545 35.0 32.0 38.0 24.0 40.0 72-73 32.81508130628623 35.0 31.0 37.5 23.0 39.5 74-75 32.40385782977966 35.0 31.0 37.0 22.0 39.0 76-77 30.868296143379464 33.5 29.5 35.5 20.5 37.5 78-79 31.71405992866546 34.0 30.5 36.0 22.0 38.0 80-81 31.69500983096941 34.0 31.0 36.0 21.5 37.0 82-83 31.423679948251596 34.0 30.5 35.0 20.5 37.0 84-85 31.132709333591308 34.0 30.0 35.0 20.0 36.5 86-87 30.843355134610462 34.0 30.0 35.0 20.0 36.0 88-89 30.579399291130613 34.0 29.5 35.0 19.0 36.0 90-91 30.29828725995162 34.0 29.0 35.0 18.5 35.0 92-93 30.087364325583227 34.0 29.0 35.0 18.0 35.0 94-95 29.86791793892923 34.0 29.0 35.0 18.0 35.0 96-97 29.659050989287543 34.0 29.0 35.0 17.0 35.0 98-99 29.497574925270555 33.5 29.0 35.0 17.0 35.0 100 27.625844169298425 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3420337347238629 1101 2 0.25427020692862357 1101 3 0.2632232546144664 1101 4 0.20019130688153552 1101 5 0.2194737461342271 1101 6 0.19441774609099838 1101 7 0.22345884031905427 1101 8 0.16112946456802746 1101 9 0.21572764698638736 1101 10-11 0.2084615581007938 1101 12-13 0.23173746097796055 1101 14-15 0.29585472061732787 1101 16-17 0.26591660797502925 1101 18-19 0.27932238347947447 1101 20-21 0.2387271479797377 1101 22-23 0.23469025112112263 1101 24-25 0.259156622089165 1101 26-27 0.25548009266394445 1101 28-29 0.2666847478207117 1101 30-31 0.39771683861107476 1101 32-33 0.3873838544718353 1101 34-35 0.4371709476408725 1101 36-37 0.4107104973773801 1101 38-39 0.40967504079731043 1101 40-41 0.3965228824045468 1101 42-43 0.38729667769506904 1101 44-45 0.3740184037619869 1101 46-47 0.4569089695429369 1101 48-49 0.4902263878096562 1101 50-51 0.3983560040087042 1101 52-53 0.441182333011362 1101 54-55 0.3360072558708538 1101 56-57 0.39706050482305955 1101 58-59 0.38993171619047473 1101 60-61 0.41372359573552586 1101 62-63 0.44691942432616827 1101 64-65 0.36162590999444433 1101 66-67 0.2223078522493651 1101 68-69 0.2574689020598342 1101 70-71 0.27859753021204625 1101 72-73 0.17900737050266713 1101 74-75 0.1197588165234933 1101 76-77 0.2765307288005623 1101 78-79 0.3361300771690843 1101 80-81 0.36396339316024395 1101 82-83 0.2912699767892626 1101 84-85 0.27730922210832176 1101 86-87 0.21079234124725943 1101 88-89 0.08433680393124376 1101 90-91 0.08461897919755046 1101 92-93 -0.06451211261386902 1101 94-95 0.13009051631052415 1101 96-97 -0.031042831184006303 1101 98-99 -0.1603842307508323 1101 100 -0.11218437903748679 1102 1 0.29388703952217554 1102 2 0.20246651441897257 1102 3 0.292056738518653 1102 4 0.21446860044104454 1102 5 0.1763026501814835 1102 6 0.11596203091813351 1102 7 0.17239206740700297 1102 8 0.12194471813025132 1102 9 0.2000527674072785 1102 10-11 0.22713296741802225 1102 12-13 0.23448065306209998 1102 14-15 0.3229686901089437 1102 16-17 0.32443162722577057 1102 18-19 0.32880301875220397 1102 20-21 0.3049444008069031 1102 22-23 0.35135587315110683 1102 24-25 0.32839592401521145 1102 26-27 0.27046882623074 1102 28-29 0.2993642976682125 1102 30-31 0.3816003216376629 1102 32-33 0.4346587287694348 1102 34-35 0.5142233100814408 1102 36-37 0.5078292037926389 1102 38-39 0.5520775280719619 1102 40-41 0.5008364444772511 1102 42-43 0.5223842158634184 1102 44-45 0.6135188240293985 1102 46-47 0.5708086773151066 1102 48-49 0.5145969000809316 1102 50-51 0.6032518927057993 1102 52-53 0.551481623543971 1102 54-55 0.6013481831296232 1102 56-57 0.5433698935456803 1102 58-59 0.5518543668357054 1102 60-61 0.6055191835613343 1102 62-63 0.539288679427564 1102 64-65 0.49279629920008716 1102 66-67 0.4581108286791249 1102 68-69 0.4921639140150873 1102 70-71 0.402742141124925 1102 72-73 0.4647273301319572 1102 74-75 0.41741202694392143 1102 76-77 0.4941645301894617 1102 78-79 0.4464132341629714 1102 80-81 0.4442150350030083 1102 82-83 0.3399135770293924 1102 84-85 0.32150871515727175 1102 86-87 0.3053280376230241 1102 88-89 0.23844982757593058 1102 90-91 0.3413632756270495 1102 92-93 0.21940469538365548 1102 94-95 0.23878790685579787 1102 96-97 0.2926405911900254 1102 98-99 0.21872654056170404 1102 100 0.24490725378851863 1103 1 0.34051147795967296 1103 2 0.2753220261592233 1103 3 0.3540081314152843 1103 4 0.17511782847920188 1103 5 0.18662198724744172 1103 6 0.20118030734697356 1103 7 0.18759061670704824 1103 8 0.22575380334406248 1103 9 0.22345094455818781 1103 10-11 0.257379642919183 1103 12-13 0.31651911621042217 1103 14-15 0.3788532937316731 1103 16-17 0.3875103990380353 1103 18-19 0.369206043421066 1103 20-21 0.346152806791558 1103 22-23 0.3649423862714585 1103 24-25 0.3335262090363216 1103 26-27 0.372458313183067 1103 28-29 0.4035316569087115 1103 30-31 0.5097334928601427 1103 32-33 0.5405273644622497 1103 34-35 0.4778362683624735 1103 36-37 0.5027070721052382 1103 38-39 0.5378182533200544 1103 40-41 0.5724262463452021 1103 42-43 0.5448790404002679 1103 44-45 0.5777367833053759 1103 46-47 0.6106078432435069 1103 48-49 0.5496719246682886 1103 50-51 0.5883572356155824 1103 52-53 0.5400197956668578 1103 54-55 0.6211031199141104 1103 56-57 0.593966239665285 1103 58-59 0.5297956603120682 1103 60-61 0.5271513354989352 1103 62-63 0.5652757172812457 1103 64-65 0.6235654261463637 1103 66-67 0.6129165348678498 1103 68-69 0.6568266855677081 1103 70-71 0.7116366509562155 1103 72-73 0.5396719596741448 1103 74-75 0.5558890346216003 1103 76-77 0.5787875738685919 1103 78-79 0.5668017493114412 1103 80-81 0.7111119329076665 1103 82-83 0.649459907160054 1103 84-85 0.4751590506308503 1103 86-87 0.5085509178363488 1103 88-89 0.5245040924889572 1103 90-91 0.650290818932806 1103 92-93 0.5604087927423897 1103 94-95 0.6014978722672808 1103 96-97 0.6187435455537802 1103 98-99 0.6632209290987028 1103 100 0.7532413000462022 1104 1 0.26908490806538765 1104 2 0.237543701506425 1104 3 0.29683855164859096 1104 4 0.09062686264343256 1104 5 0.16116060185035508 1104 6 0.17789213760134714 1104 7 0.1715229958451303 1104 8 0.14573741169063226 1104 9 0.21218977646702797 1104 10-11 0.18849755373564392 1104 12-13 0.2531838072975887 1104 14-15 0.31860077770905093 1104 16-17 0.22031855770436692 1104 18-19 0.27576841857071344 1104 20-21 0.15824322561676496 1104 22-23 0.26589592986390187 1104 24-25 0.2513241289145469 1104 26-27 0.3743807040059721 1104 28-29 0.3710579026739751 1104 30-31 0.2548795143560838 1104 32-33 0.3043023134520837 1104 34-35 0.36144583223173044 1104 36-37 0.30699833106915264 1104 38-39 0.3140586945090007 1104 40-41 0.40160764351288236 1104 42-43 0.4960949210878738 1104 44-45 0.39268382909458666 1104 46-47 0.43182633084108346 1104 48-49 0.4264690366676902 1104 50-51 0.4514710158313022 1104 52-53 0.3955185497275906 1104 54-55 0.521882122269659 1104 56-57 0.5718118320311518 1104 58-59 0.5782097657568173 1104 60-61 0.4866272186102165 1104 62-63 0.4101548908754111 1104 64-65 0.576503102960281 1104 66-67 0.49713075014116015 1104 68-69 0.4619634372974204 1104 70-71 0.360300520323257 1104 72-73 0.5280831748661612 1104 74-75 0.5963647069331515 1104 76-77 0.49609558704478474 1104 78-79 0.5864821600317889 1104 80-81 0.6108701844319704 1104 82-83 0.5004241601711321 1104 84-85 0.5111291166001948 1104 86-87 0.5395852933628085 1104 88-89 0.575850665358999 1104 90-91 0.3630551530471706 1104 92-93 0.42768878052867976 1104 94-95 0.4375879066638433 1104 96-97 0.40922946851368636 1104 98-99 0.4632612513566663 1104 100 0.56148804505154 1105 1 0.28095169849385826 1105 2 0.21853559562842406 1105 3 0.2842401348980417 1105 4 0.1558523515751915 1105 5 0.11064806382497494 1105 6 0.14904731515926528 1105 7 0.19055011259035837 1105 8 0.1994208880263102 1105 9 0.21401702349510998 1105 10-11 0.2238331933845501 1105 12-13 0.24810598076330592 1105 14-15 0.32232041584728677 1105 16-17 0.35779188706400333 1105 18-19 0.3822818204753915 1105 20-21 0.2700558271081732 1105 22-23 0.37647036897229214 1105 24-25 0.4301168745906381 1105 26-27 0.4215801470406646 1105 28-29 0.4581152352278721 1105 30-31 0.5292842704290024 1105 32-33 0.5526700008297354 1105 34-35 0.5998259109245012 1105 36-37 0.5542986219752422 1105 38-39 0.5645358845350188 1105 40-41 0.6080976163822953 1105 42-43 0.58912883485538 1105 44-45 0.6612608117826682 1105 46-47 0.7096817451772282 1105 48-49 0.7964529854951081 1105 50-51 0.8189096325687473 1105 52-53 0.8236190648557127 1105 54-55 0.7563018602866336 1105 56-57 0.794057432840809 1105 58-59 0.7995201301391219 1105 60-61 0.7528516728569414 1105 62-63 0.7327778441238877 1105 64-65 0.7231832678737149 1105 66-67 0.750093080028087 1105 68-69 0.8206151834070781 1105 70-71 0.8344752130408679 1105 72-73 0.7950728297947549 1105 74-75 0.7768340863071899 1105 76-77 0.8105936514278476 1105 78-79 0.844378858749554 1105 80-81 0.8166402932896197 1105 82-83 0.809824404747534 1105 84-85 0.7706497310314191 1105 86-87 0.8041202098871771 1105 88-89 0.7490135391850679 1105 90-91 0.7493697288941448 1105 92-93 0.8224932353058989 1105 94-95 0.9451716363246376 1105 96-97 0.8781157946527074 1105 98-99 0.8981831231974517 1105 100 0.9457053717630401 1106 1 0.22456209419394213 1106 2 0.18515246170669286 1106 3 0.21612933875918827 1106 4 0.19159227863490003 1106 5 0.17430892386176566 1106 6 0.14175182579622003 1106 7 0.1350848897417265 1106 8 0.19306350277033602 1106 9 0.18821521893668347 1106 10-11 0.1481982942767317 1106 12-13 0.2035434079346885 1106 14-15 0.28650953973746596 1106 16-17 0.27415171612058487 1106 18-19 0.25697499503079513 1106 20-21 0.3122182490459693 1106 22-23 0.3342486768403674 1106 24-25 0.31313152600744587 1106 26-27 0.2998451537136688 1106 28-29 0.366440887280298 1106 30-31 0.39734531464134903 1106 32-33 0.37900147070426726 1106 34-35 0.42038948579896385 1106 36-37 0.430624792568878 1106 38-39 0.4553633986350505 1106 40-41 0.46674564865565316 1106 42-43 0.43537742572841154 1106 44-45 0.5613686368205535 1106 46-47 0.5764365093475519 1106 48-49 0.5314797432140352 1106 50-51 0.6011002823014593 1106 52-53 0.6993245042536955 1106 54-55 0.5691696530091264 1106 56-57 0.6368760896964503 1106 58-59 0.6798690826771363 1106 60-61 0.6966506820811915 1106 62-63 0.7209824915494494 1106 64-65 0.7258898427484652 1106 66-67 0.7511492865663172 1106 68-69 0.6890498050252845 1106 70-71 0.7718020922490822 1106 72-73 0.7803706099654093 1106 74-75 0.7369528369148597 1106 76-77 0.7134084892975885 1106 78-79 0.8226810041845987 1106 80-81 0.7728273570296089 1106 82-83 0.7857763264010948 1106 84-85 0.776894561312826 1106 86-87 0.7183492316691193 1106 88-89 0.8564984303950638 1106 90-91 0.8007206367646127 1106 92-93 0.9127194051112077 1106 94-95 1.0090472413258738 1106 96-97 0.8435055049382463 1106 98-99 0.891960774713823 1106 100 0.9814896784754907 1107 1 0.13674396951167722 1107 2 0.12047971175899619 1107 3 0.2239717464787674 1107 4 0.14702329914954504 1107 5 0.06912974309244646 1107 6 0.11113561708626207 1107 7 0.08406670021481943 1107 8 0.14362024772128024 1107 9 0.1736478157177075 1107 10-11 0.16018600532456873 1107 12-13 0.1654490397830699 1107 14-15 0.2519920241670519 1107 16-17 0.218706077291408 1107 18-19 0.16938072518176028 1107 20-21 0.23563276875075445 1107 22-23 0.2711298635126269 1107 24-25 0.3094217141746185 1107 26-27 0.2562403224721663 1107 28-29 0.2951732741887554 1107 30-31 0.20969801874549177 1107 32-33 0.2944143523315006 1107 34-35 0.3321208925083923 1107 36-37 0.40758546720979894 1107 38-39 0.4064245134916433 1107 40-41 0.3114764924050277 1107 42-43 0.31321911884128184 1107 44-45 0.34873992436870793 1107 46-47 0.3652117585241612 1107 48-49 0.4341928485710582 1107 50-51 0.37161651456978007 1107 52-53 0.33034542934618827 1107 54-55 0.2794913192621351 1107 56-57 0.34833366531177745 1107 58-59 0.40514316724051724 1107 60-61 0.43178671979546124 1107 62-63 0.45129292252144637 1107 64-65 0.4326817487035015 1107 66-67 0.3972279590298271 1107 68-69 0.3833182487621869 1107 70-71 0.4438707036263523 1107 72-73 0.41436665290888897 1107 74-75 0.4206057694240428 1107 76-77 0.3841823414982777 1107 78-79 0.46568844433043566 1107 80-81 0.4841408424900031 1107 82-83 0.487019755668495 1107 84-85 0.4245971088925451 1107 86-87 0.397767568988332 1107 88-89 0.3631388500564867 1107 90-91 0.23112888377727003 1107 92-93 0.4486362206497425 1107 94-95 0.6709853987881758 1107 96-97 0.677607450476085 1107 98-99 0.6858893195334588 1107 100 0.651178233626343 1108 1 0.19708490763875375 1108 2 0.17419931061257898 1108 3 0.20184787000781057 1108 4 0.14680849066756707 1108 5 0.11731078426183927 1108 6 0.1212155071577996 1108 7 0.14092670538216368 1108 8 0.13603620153983798 1108 9 0.1416521183526953 1108 10-11 0.19733272296959825 1108 12-13 0.1952412996261046 1108 14-15 0.23231451522876512 1108 16-17 0.26040016804184063 1108 18-19 0.2599345406787208 1108 20-21 0.27785875773889757 1108 22-23 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2316 72-73 -0.4173044749201722 2316 74-75 -0.4489735359333942 2316 76-77 -0.4259156275567584 2316 78-79 -0.2387785252733785 2316 80-81 -0.25548992939029347 2316 82-83 -0.19970437113507344 2316 84-85 -0.1468520157383182 2316 86-87 -0.14805750030010856 2316 88-89 -0.09751891323060846 2316 90-91 -0.04602321503096718 2316 92-93 0.06028465358596691 2316 94-95 0.1157547969127215 2316 96-97 0.036021243465402364 2316 98-99 0.08444564222468998 2316 100 -0.08835172056787499 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 4.0 10 11.0 11 31.0 12 62.0 13 217.0 14 594.0 15 1551.0 16 3598.0 17 7487.0 18 13099.0 19 20440.0 20 28180.0 21 38188.0 22 49065.0 23 56127.0 24 59328.0 25 64488.0 26 73832.0 27 87285.0 28 105761.0 29 127014.0 30 152593.0 31 184349.0 32 226079.0 33 282522.0 34 373990.0 35 507885.0 36 735376.0 37 1127977.0 38 1057045.0 39 156457.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.718418564725184 19.703036881602085 13.667790154148932 23.910754399523796 2 15.473745426174023 21.07563022174703 39.34564940201628 24.10497495006267 3 17.471197608778404 26.57064696434447 30.84518478958683 25.1129706372903 4 12.267077354241678 17.223429767064268 37.29438924511612 33.21510363357793 5 12.662605697990273 38.02591800453023 35.07837669545442 14.23309960202507 6 31.10238323767005 37.160719010564534 17.85708226561172 13.879815486153701 7 27.048557805630885 32.97666665343117 22.39625502693364 17.578520514004303 8 26.259736303117855 35.807756699450096 20.158849107425834 17.773657890006216 9 26.9913540861256 14.925806565725477 20.157356932000063 37.92548241614886 10-11 24.881927295947374 26.1455506876986 28.814504569316185 20.15801744703784 12-13 25.22356566571383 24.065218654693112 28.703759520085537 22.007456159507523 14-15 22.748415620249883 25.392859759876725 26.361653335508766 25.497071284364626 16-17 22.21577388069736 28.141325466577268 27.186115620738853 22.45678503198652 18-19 22.102901074124965 27.15682751498348 29.1797391005997 21.56053231029186 20-21 22.792617327682567 26.530889793622837 29.162400344951628 21.514092533742975 22-23 22.622744535211908 26.29942241475465 28.999410538900282 22.078422511133162 24-25 22.305656030074434 26.464091867871893 29.040963222205036 22.18928887984864 26-27 22.232015502882604 26.494153119662737 29.09755846203521 22.176272915419446 28-29 22.249454504566334 26.742844261335698 29.108704815746222 21.89899641835175 30-31 22.75220369886064 26.487179750967904 29.08068500520975 21.6799315449617 32-33 22.497308691484854 26.578441502021334 29.02640645405066 21.89784335244315 34-35 22.63301104612142 26.756889758894197 28.933174489479786 21.676924705504597 36-37 22.60186572592246 26.737246547201394 28.833408716563508 21.827479010312643 38-39 22.565582092111377 26.87674113246369 28.74369537457755 21.813981400847386 40-41 22.563655495932583 26.676377209464352 28.862837680515923 21.897129614087145 42-43 22.408899653638233 26.90247857270687 28.582647653635707 22.105974120019194 44-45 22.289562137632192 27.008288687649273 28.628474658563736 22.073674516154796 46-47 22.30987908575183 26.81198096128542 28.39727703784459 22.48086291511816 48-49 22.398860037347152 26.957022978398815 28.351083804745386 22.29303317950865 50-51 22.415585182120708 27.21570423950606 28.199534113072566 22.169176465300666 52-53 22.457488079479525 27.320093003343054 27.922526453646512 22.299892463530917 54-55 22.311872690879753 27.16476582565206 28.15927357130094 22.364087912167246 56-57 22.43681503483699 27.26919626121668 27.820823283883694 22.47316542006263 58-59 22.433507737738395 27.2052913147978 28.077797654731977 22.283403292731823 60-61 22.67914312154562 27.27527228469334 28.060372376765013 21.985212216996022 62-63 22.536036262483325 27.28572626366655 28.069976563827716 22.10826091002241 64-65 22.360769068257973 27.07571567588124 28.057640501587915 22.505874754272874 66-67 22.314020423056505 27.080071139368865 27.94017123483627 22.665737202738356 68-69 22.14831227436823 27.163294223826718 28.077066787003613 22.611326714801443 70-71 22.2097631205396 27.047932625911052 28.065097698258928 22.677206555290418 72-73 22.17255134767018 27.4337432459007 27.646576736251717 22.747128670177403 74-75 22.252458221647945 27.40814032840784 27.605397285329868 22.73400416461435 76-77 22.116697497145122 27.23372366421665 27.77414293586172 22.875435902776513 78-79 22.02579150756164 27.152316386607822 27.85383903876271 22.968053067067828 80-81 22.077649461518433 27.426817541679473 27.520827890308325 22.97470510649377 82-83 22.216400699810173 27.32856383673888 27.605325330138985 22.84971013331196 84-85 22.12115222326018 27.505896050796746 27.407587921783122 22.965363804159956 86-87 21.921194626922794 27.48355889642872 27.606467766771914 22.988778709876577 88-89 21.829874821306753 27.265245496857943 27.61438218214174 23.290497499693565 90-91 22.197468976905377 27.19136626540955 27.548953927913082 23.06221082977199 92-93 22.007279153308705 27.252916725568987 27.503249331853596 23.23655478926871 94-95 21.785822293439256 27.12668758040127 27.491317790967972 23.596172335191497 96-97 21.751947653036456 27.049069275891252 27.61684496590665 23.582138105165637 98-99 21.63814658699078 27.029699386798207 27.455950405920472 23.876203620290546 100 20.804194194694723 27.53601892680359 27.689981527737185 23.9698053507645 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3336.0 1 2409.0 2 2695.5 3 3692.5 4 2653.0 5 2173.5 6 3227.5 7 5907.5 8 9066.5 9 10442.5 10 8841.5 11 6806.0 12 6790.5 13 7195.0 14 7623.0 15 7998.5 16 8415.5 17 8968.5 18 9539.0 19 10152.0 20 11045.5 21 12321.5 22 14213.0 23 17122.5 24 20545.0 25 24601.5 26 30148.0 27 36539.5 28 43166.5 29 51059.0 30 60148.0 31 68854.0 32 79044.5 33 91511.0 34 103819.0 35 114166.0 36 127569.5 37 140926.0 38 150665.0 39 159815.5 40 164249.0 41 167277.5 42 172215.5 43 178166.0 44 184696.5 45 192574.0 46 198892.5 47 203421.5 48 209717.5 49 214890.5 50 218424.5 51 215579.0 52 207283.5 53 199800.0 54 190915.0 55 177609.0 56 162236.0 57 146696.0 58 129952.5 59 111231.0 60 91466.5 61 74409.0 62 60067.0 63 46468.0 64 34628.0 65 25971.5 66 19285.0 67 14373.0 68 10854.0 69 8291.5 70 6403.0 71 4827.5 72 3678.5 73 2875.0 74 2215.0 75 1621.0 76 1164.0 77 815.5 78 551.0 79 388.5 80 272.0 81 183.0 82 137.0 83 94.5 84 67.0 85 51.5 86 36.0 87 26.0 88 17.0 89 10.5 90 8.5 91 7.5 92 4.5 93 1.5 94 1.0 95 1.5 96 1.5 97 1.0 98 2.0 99 2.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0030321330297831267 2 0.06703179805127697 3 9.926625990361426E-4 4 4.8730709407228824E-4 5 0.0018770347327228879 6 7.219364356626492E-4 7 0.0 8 9.024205445783116E-4 9 3.609682178313246E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.038880247710829E-4 18-19 9.204689554698777E-4 20-21 0.0030140846188915606 22-23 0.0 24-25 1.804841089156623E-5 26-27 1.263388762409636E-4 28-29 8.753479282409622E-4 30-31 0.001498018103999997 32-33 0.0024094628540240917 34-35 0.0036548032055421613 36-37 0.0013716792277590336 38-39 0.0014258244604337322 40-41 0.00512574869320481 42-43 0.006289871195710832 44-45 0.004900143557060231 46-47 0.006569621564530109 48-49 0.008527874146265043 50-51 0.008455680502698779 52-53 0.0037540694654457757 54-55 0.002238002950554213 56-57 0.0024365354703614413 58-59 0.003221641344144572 60-61 0.007309606411084323 62-63 0.012877541171132505 64-65 0.0037811420817831253 66-67 0.009800287114120463 68-69 0.011803660723084313 70-71 0.005495741116481917 72-73 0.007354727438313239 74-75 0.002977987797108428 76-77 0.0025448259357108384 78-79 0.007571308369012033 80-81 0.005531837938265049 82-83 0.015629923832096358 84-85 0.01676697371826503 86-87 0.021369318495614416 88-89 0.02044884954014454 90-91 0.023697563500626462 92-93 0.03290225305532524 94-95 0.03662022569898788 96-97 0.022208569602072247 98-99 0.017335498661349365 100 0.01799426565889153 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5540654.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.70937402572959 #Duplication Level Percentage of deduplicated Percentage of total 1 74.62707368860502 44.559358553186016 2 15.615048260922382 18.647295140824657 3 4.7643082414238425 8.534215882831267 4 1.8825015335259359 4.496119526772385 5 0.9190613024872105 2.7438287531391525 6 0.5585135837570204 2.000909788259915 7 0.3594422361714747 1.5023449644145048 8 0.23160448599636915 1.1063167104315246 9 0.17482363166163656 0.9394748650278935 >10 0.7969109269423005 8.234974677606846 >50 0.044378769948196374 1.8172299778056475 >100 0.024020713312866038 2.6116790636264686 >500 0.001674659804328102 0.6609529533017066 >1k 5.183469211407746E-4 0.7430897784261581 >5k 0.0 0.0 >10k+ 1.1961852026324674E-4 1.4022093643458922 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22973 0.41462614341195103 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21262 0.3837453123764812 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14662 0.26462580049214407 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.8048410891566232E-5 1.8048410891566232E-5 5 0.0 0.0 0.0 1.8048410891566232E-5 1.8048410891566232E-5 6 0.0 0.0 0.0 1.8048410891566232E-5 1.263388762409636E-4 7 0.0 0.0 0.0 1.8048410891566232E-5 1.263388762409636E-4 8 0.0 0.0 0.0 1.8048410891566232E-5 1.263388762409636E-4 9 0.0 0.0 0.0 1.8048410891566232E-5 1.804841089156623E-4 10-11 0.0 0.0 0.0 1.8048410891566232E-5 1.804841089156623E-4 12-13 0.0 0.0 0.0 1.8048410891566232E-5 1.9853251980722853E-4 14-15 0.0 0.0 0.0 1.8048410891566232E-5 2.0755672525301163E-4 16-17 0.0 0.0 0.0 1.8048410891566232E-5 2.3462934159036098E-4 18-19 0.0 0.0 0.0 1.8048410891566232E-5 2.3462934159036098E-4 20-21 0.0 0.0 0.0 3.6096821783132464E-5 2.3462934159036098E-4 22-23 0.0 0.0 0.0 1.263388762409636E-4 2.526777524819272E-4 24-25 0.0 0.0 0.0 2.8877457426505966E-4 2.7072616337349344E-4 26-27 0.0 0.0 1.8048410891566232E-5 6.046217648674687E-4 2.7072616337349344E-4 28-29 0.0 0.0 1.8048410891566232E-5 0.001958252581734936 2.7072616337349344E-4 30-31 0.0 0.0 1.8048410891566232E-5 0.007056928658602397 2.7072616337349344E-4 32-33 0.0 0.0 1.8048410891566232E-5 0.016559416993012017 2.7072616337349344E-4 34-35 0.0 0.0 1.8048410891566232E-5 0.0315305738275662 3.068229851566259E-4 36-37 0.0 0.0 1.8048410891566232E-5 0.055706420216819166 3.068229851566259E-4 38-39 0.0 0.0 1.8048410891566232E-5 0.09392393027971066 3.2487139604819214E-4 40-41 0.0 0.0 1.8048410891566232E-5 0.1382688758402889 3.4291980693975835E-4 42-43 0.0 0.0 1.8048410891566232E-5 0.18218065953946955 3.4291980693975835E-4 44-45 0.0 0.0 1.8048410891566232E-5 0.23629881959783086 3.5194401238554146E-4 46-47 0.0 0.0 1.8048410891566232E-5 0.300704573864385 3.7901662872289084E-4 48-49 0.0 0.0 1.8048410891566232E-5 0.3707504565345535 3.7901662872289084E-4 50-51 0.0 0.0 1.8048410891566232E-5 0.44677036320983043 3.7901662872289084E-4 52-53 0.0 0.0 1.8048410891566232E-5 0.5242160943455412 4.2413765595180643E-4 54-55 0.0 0.0 1.8048410891566232E-5 0.6159651911128181 4.6925868318072197E-4 56-57 0.0 0.0 1.8048410891566232E-5 0.7227756867691071 4.6925868318072197E-4 58-59 0.0 0.0 1.8048410891566232E-5 0.8467051001560466 4.6925868318072197E-4 60-61 0.0 0.0 1.8048410891566232E-5 0.9776824179961427 4.6925868318072197E-4 62-63 0.0 0.0 1.8048410891566232E-5 1.1136230488314194 4.8730709407228824E-4 64-65 0.0 0.0 1.8048410891566232E-5 1.2541028550059252 4.8730709407228824E-4 66-67 0.0 0.0 1.8048410891566232E-5 1.4167009887280455 4.963312995180714E-4 68-69 0.0 0.0 1.8048410891566232E-5 1.6165600667358042 5.053555049638545E-4 70-71 0.0 0.0 1.8048410891566232E-5 1.8347021849767193 5.053555049638545E-4 72-73 0.0 0.0 1.8048410891566232E-5 2.0425747574203332 6.136459703132518E-4 74-75 0.0 0.0 1.8048410891566232E-5 2.2535697049481884 6.136459703132518E-4 76-77 0.0 0.0 1.8048410891566232E-5 2.4900219360385973 6.136459703132518E-4 78-79 0.0 0.0 1.8048410891566232E-5 2.764096079632477 6.136459703132518E-4 80-81 0.0 0.0 1.8048410891566232E-5 3.0553613346005726 6.407185866506011E-4 82-83 0.0 0.0 1.8048410891566232E-5 3.36053289016062 6.677912029879505E-4 84-85 0.0 0.0 1.8048410891566232E-5 3.6715340824386438 6.677912029879505E-4 86-87 0.0 0.0 1.8048410891566232E-5 3.999058594887896 6.677912029879505E-4 88 0.0 0.0 1.8048410891566232E-5 4.264586815924618 6.677912029879505E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11255 0.0 47.29205 1 GTATCAA 18030 0.0 34.50256 1 TACCTGG 4815 0.0 32.22443 2 GTACCTG 5465 0.0 29.167332 1 TCAACGC 22780 0.0 26.85923 4 ATCAACG 22790 0.0 26.806202 3 CAACGCA 22965 0.0 26.683783 5 GTACATG 20540 0.0 26.50912 1 AACGCAG 23570 0.0 26.05773 6 TATCAAC 23605 0.0 26.033895 2 TACATGG 21220 0.0 25.39258 2 CATGGGG 12485 0.0 24.87993 4 ACATGGG 21115 0.0 24.793074 3 ACCTGGG 6185 0.0 24.617365 3 ATGGGGG 7930 0.0 24.00038 5 ACGCAGA 27250 0.0 22.435074 7 CGCAGAG 27730 0.0 21.995888 8 TGGGGGG 7570 0.0 20.73343 6 GAGTACT 18475 0.0 20.119835 12-13 GCAGAGT 30340 0.0 19.809237 9 >>END_MODULE