FastQCFastQC Report
Mon 6 Jun 2016
ERR1211539_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211539_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9094088
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT418670.46037601571482484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT361310.39730207141166873No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242150.26627189004548885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA197100.052.0885541
GTATCAA361200.038.3935471
GTACATG441950.030.6649191
ATCAACG436000.030.618973
TCAACGC436450.030.5754554
CAACGCA440400.030.3959065
AACGCAG455600.029.448836
TACATGG455850.029.3341732
ACATGGG439650.029.0287593
TATCAAC467650.028.7045962
ACGCAGA509100.026.1799557
CGCAGAG510800.025.946388
CATGGGA247600.025.4677724
CATGGGG147350.024.809534
TACCTGG55200.024.531252
GCAGAGT554150.023.3687889
GAGTACT324450.021.68838712-13
CATTCCG14850.021.1998449
AGAGTAC512650.021.08546610-11
CAGAGTA552350.020.31004710-11
Produced by FastQC (version 0.11.5)