FastQCFastQC Report
Mon 6 Jun 2016
ERR1211539_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211539_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9094088
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT469970.5167862901700533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT406690.4472026221870737No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT322450.35457101360796156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA215100.047.6351551
GTATCAA376150.036.978381
GTACATG435650.029.0460281
ATCAACG481600.028.2393863
TCAACGC484350.028.1468184
CAACGCA488900.027.8943255
TACATGG448950.027.8181922
ACATGGG440850.027.161383
AACGCAG504900.027.1114086
TATCAAC509100.026.8867242
ACGCAGA570450.023.8230677
CATGGGA246900.023.6396684
CGCAGAG574700.023.556958
TACCTGG54850.022.7178962
CATGGGG148800.022.7073694
GCAGAGT622400.021.3136719
AGAGTAC572300.021.14758310-11
GAGTACT365350.020.69595112-13
AGTACTT402000.020.34634812-13
ATGGGAT119800.019.5741185
Produced by FastQC (version 0.11.5)