##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211539_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9094088 Sequences flagged as poor quality 0 Sequence length 100 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.838227538594303 31.0 31.0 34.0 30.0 34.0 2 32.08413740883088 33.0 31.0 34.0 30.0 34.0 3 32.087992110918655 33.0 31.0 34.0 30.0 34.0 4 35.81986923812481 37.0 35.0 37.0 35.0 37.0 5 35.73308549466422 37.0 35.0 37.0 35.0 37.0 6 35.756312122776905 37.0 35.0 37.0 33.0 37.0 7 35.67204660874186 37.0 35.0 37.0 33.0 37.0 8 35.661351638559026 37.0 35.0 37.0 33.0 37.0 9 37.249247423161066 39.0 37.0 39.0 34.0 39.0 10-11 37.19084002705934 39.0 37.0 39.0 33.0 39.0 12-13 37.10815037197793 39.0 37.0 39.0 33.0 39.0 14-15 38.22251412126208 40.0 38.0 41.0 33.0 41.0 16-17 38.15794739395528 40.0 37.0 41.0 33.0 41.0 18-19 38.1829837142548 40.0 37.5 41.0 33.0 41.0 20-21 38.139406447353494 40.0 37.5 41.0 33.0 41.0 22-23 38.02533453601944 40.0 37.0 41.0 33.0 41.0 24-25 37.90856609260874 40.0 37.0 41.0 33.0 41.0 26-27 37.70332176244611 39.0 37.0 41.0 32.0 41.0 28-29 37.726443047395186 40.0 37.0 41.0 32.5 41.0 30-31 37.60962803526863 39.0 37.0 41.0 32.5 41.0 32-33 37.392949628373955 39.0 36.0 41.0 32.0 41.0 34-35 37.269799291583716 39.0 36.0 41.0 31.5 41.0 36-37 37.19241896493634 39.0 36.0 41.0 31.0 41.0 38-39 37.084461960341706 39.0 36.0 41.0 31.0 41.0 40-41 36.91003193503296 39.0 35.0 40.0 30.5 41.0 42-43 36.73473117920126 39.0 35.0 40.0 30.0 41.0 44-45 36.51065516410222 39.0 35.0 40.0 30.0 41.0 46-47 36.19708485336847 39.0 35.0 40.0 29.0 41.0 48-49 35.9289564824972 38.5 35.0 40.0 28.0 41.0 50-51 35.71735076678387 38.0 35.0 40.0 27.0 41.0 52-53 35.52494059877142 38.0 34.0 40.0 26.5 41.0 54-55 35.328928585252314 38.0 34.0 40.0 26.0 41.0 56-57 35.137099728966774 38.0 34.0 40.0 26.0 41.0 58-59 34.88545745323775 38.0 33.5 40.0 25.5 41.0 60-61 34.57664996204127 38.0 33.0 40.0 25.0 41.0 62-63 34.262237290864135 37.0 33.0 40.0 23.5 41.0 64-65 33.95958005904495 37.0 32.0 39.0 23.0 40.5 66-67 33.629410722658506 36.0 32.0 39.0 22.5 40.0 68-69 33.22728337354994 36.0 31.0 39.0 22.0 40.0 70-71 32.84507643867092 35.0 31.0 38.5 22.0 40.0 72-73 32.435074962986945 35.0 31.0 38.0 21.0 40.0 74-75 32.0096092098515 35.0 30.0 37.0 20.0 39.0 76-77 30.52084859966167 33.0 28.5 35.5 19.5 38.5 78-79 31.4036325577672 34.0 30.0 36.0 20.0 39.0 80-81 31.374998020692125 34.0 30.0 36.0 20.0 38.0 82-83 31.06216813604619 34.0 30.0 35.5 19.5 37.0 84-85 30.71867069023304 34.0 29.0 35.0 19.0 37.0 86-87 30.398389811050873 34.0 29.0 35.0 18.0 36.0 88-89 30.067630036128968 34.0 29.0 35.0 18.0 36.0 90-91 29.738575764826557 33.5 29.0 35.0 17.5 35.5 92-93 29.48935852611059 33.0 28.5 35.0 16.5 35.0 94-95 29.228114737838474 33.0 27.0 35.0 15.0 35.0 96-97 28.993984993327533 33.0 27.0 35.0 12.5 35.0 98-99 28.80511932587413 33.0 26.5 34.0 11.5 35.0 100 26.898508349600313 31.0 24.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2073194904563742 1101 2 0.16564960985036237 1101 3 0.20785677986315676 1101 4 0.11087537396281988 1101 5 0.14062134373322976 1101 6 0.1315916939095274 1101 7 0.1508097232158292 1101 8 0.11106143647172928 1101 9 0.16046486394336767 1101 10-11 0.13917722944891864 1101 12-13 0.13666620972426813 1101 14-15 0.17731617737516814 1101 16-17 0.16115453584875894 1101 18-19 0.20600082633483652 1101 20-21 0.17366801761795614 1101 22-23 0.17033634508293716 1101 24-25 0.08548387157705406 1101 26-27 0.11912639727996321 1101 28-29 0.17259733541253297 1101 30-31 0.2150388970587116 1101 32-33 0.2540265294443316 1101 34-35 0.21480213398266557 1101 36-37 0.31153792927600676 1101 38-39 0.2871571597093805 1101 40-41 0.2802818994740761 1101 42-43 0.271023447663147 1101 44-45 0.2606573053754957 1101 46-47 0.37709176998549054 1101 48-49 0.3510940725324829 1101 50-51 0.3177079801987617 1101 52-53 0.28428868368209237 1101 54-55 0.2672136917096708 1101 56-57 0.27941268514845063 1101 58-59 0.2734802842437887 1101 60-61 0.1488546086265785 1101 62-63 0.14687817652887958 1101 64-65 0.1308750064094255 1101 66-67 0.020930743905296367 1101 68-69 0.034716020333512176 1101 70-71 0.01886658978623501 1101 72-73 -0.13600015510685637 1101 74-75 -0.003812416636666427 1101 76-77 0.11875243947816827 1101 78-79 0.21072615989007915 1101 80-81 0.1613781406020891 1101 82-83 0.18226362336888258 1101 84-85 0.1070971258235538 1101 86-87 0.07297293502858793 1101 88-89 0.027799153104794527 1101 90-91 -0.0644893866323244 1101 92-93 -0.11493951317390838 1101 94-95 -0.06353394326156447 1101 96-97 -0.14247831475287498 1101 98-99 -0.27225672322020955 1101 100 -0.13723012868504014 1102 1 0.22615312523938158 1102 2 0.1809885492416825 1102 3 0.270889361549024 1102 4 0.15862765892666175 1102 5 0.1552112300965902 1102 6 0.11017615500234257 1102 7 0.11187548989871487 1102 8 0.13822382312952897 1102 9 0.15878843104844975 1102 10-11 0.18201636857124015 1102 12-13 0.21234758585851665 1102 14-15 0.2591937393885573 1102 16-17 0.25904973658047226 1102 18-19 0.18444050819012858 1102 20-21 0.28957023961505257 1102 22-23 0.2101574458190214 1102 24-25 0.21954476111716303 1102 26-27 0.20825807601696766 1102 28-29 0.25556622658837114 1102 30-31 0.3035988663948572 1102 32-33 0.388017580054111 1102 34-35 0.34848795649963193 1102 36-37 0.3603568241170194 1102 38-39 0.34531841037136957 1102 40-41 0.3312042397366355 1102 42-43 0.27213207826621755 1102 44-45 0.3011670765455321 1102 46-47 0.4002865208900488 1102 48-49 0.3641502307128448 1102 50-51 0.3196329443501327 1102 52-53 0.33488969897448584 1102 54-55 0.4249018887605729 1102 56-57 0.3682753807921699 1102 58-59 0.41234498234523187 1102 60-61 0.4328191485493065 1102 62-63 0.4631537976922928 1102 64-65 0.39379973850220296 1102 66-67 0.35807993287388484 1102 68-69 0.41074574174788125 1102 70-71 0.36021880881825297 1102 72-73 0.4695998852099734 1102 74-75 0.3410575131815641 1102 76-77 0.393109022529611 1102 78-79 0.3853094633667169 1102 80-81 0.4101116658830932 1102 82-83 0.4698456881014934 1102 84-85 0.3055984947015915 1102 86-87 0.2852491282051197 1102 88-89 0.3221941864331086 1102 90-91 0.26762970060935487 1102 92-93 0.1443858140348908 1102 94-95 0.24539793446517066 1102 96-97 0.21759287905373625 1102 98-99 0.16462087276803672 1102 100 0.24091411086696723 1103 1 0.21858119025824507 1103 2 0.18010697816354337 1103 3 0.2592291580827393 1103 4 0.11986799540164128 1103 5 0.13584321296873014 1103 6 0.18390347227941106 1103 7 0.15746836228120742 1103 8 0.15278788314795833 1103 9 0.19481531143943442 1103 10-11 0.2081778784890247 1103 12-13 0.18879822106539024 1103 14-15 0.2672936317715724 1103 16-17 0.28004117587062183 1103 18-19 0.24389494739789797 1103 20-21 0.20958128536407372 1103 22-23 0.22656456807946057 1103 24-25 0.20237941028034356 1103 26-27 0.26604260278655545 1103 28-29 0.2844316423060178 1103 30-31 0.40954468717963977 1103 32-33 0.36927256688369425 1103 34-35 0.30794408588537436 1103 36-37 0.44202445315385575 1103 38-39 0.3977975744118467 1103 40-41 0.4033053161578266 1103 42-43 0.39749915989926166 1103 44-45 0.37825312419082735 1103 46-47 0.5022617160126828 1103 48-49 0.4767149306318217 1103 50-51 0.4645763616977021 1103 52-53 0.410049597803841 1103 54-55 0.444999013155325 1103 56-57 0.501994583761558 1103 58-59 0.581751275567143 1103 60-61 0.47909432646493855 1103 62-63 0.5507702821516105 1103 64-65 0.5290162751120278 1103 66-67 0.4982215992718295 1103 68-69 0.4724999753789447 1103 70-71 0.5070433848945228 1103 72-73 0.4516334959145212 1103 74-75 0.361932783882569 1103 76-77 0.3915666261284372 1103 78-79 0.5010452143411257 1103 80-81 0.4693293088742756 1103 82-83 0.4565770072590212 1103 84-85 0.40796564396251966 1103 86-87 0.4666862130227152 1103 88-89 0.4498818886380356 1103 90-91 0.4152328071232283 1103 92-93 0.31016040484207963 1103 94-95 0.37861523348230364 1103 96-97 0.4123053670125181 1103 98-99 0.32182377483266933 1103 100 0.41562223177133717 1104 1 0.25183337678283024 1104 2 0.1890120626503844 1104 3 0.2696517013297708 1104 4 0.05598041313727009 1104 5 0.14856062641652557 1104 6 0.13660703774677785 1104 7 0.14160572705332442 1104 8 0.13599594073356513 1104 9 0.2369794144519588 1104 10-11 0.24033508955248095 1104 12-13 0.2825036173230018 1104 14-15 0.336194078559636 1104 16-17 0.27750241046593516 1104 18-19 0.2823899970911512 1104 20-21 0.14973332271124917 1104 22-23 0.25063519629384956 1104 24-25 0.3083040627139155 1104 26-27 0.3352714042292746 1104 28-29 0.39306467671605105 1104 30-31 0.29453773166506636 1104 32-33 0.318566813935675 1104 34-35 0.4655820098789931 1104 36-37 0.3200842775042432 1104 38-39 0.467887652434122 1104 40-41 0.5670552196467611 1104 42-43 0.5669248904143345 1104 44-45 0.5160617799951268 1104 46-47 0.50372347484263 1104 48-49 0.5000238475715335 1104 50-51 0.4713220842293637 1104 52-53 0.5134842545712388 1104 54-55 0.5593810317832251 1104 56-57 0.5055795393850673 1104 58-59 0.5737291987979845 1104 60-61 0.4881484384975536 1104 62-63 0.48607668396402914 1104 64-65 0.5294166500142126 1104 66-67 0.6009218727689287 1104 68-69 0.5763040459906179 1104 70-71 0.5485042569079823 1104 72-73 0.6373160970609604 1104 74-75 0.6866072173251787 1104 76-77 0.5633054541270717 1104 78-79 0.6829688815608677 1104 80-81 0.7802063972469853 1104 82-83 0.5996507431553617 1104 84-85 0.5930064711121688 1104 86-87 0.7240908980803837 1104 88-89 0.6970767320379245 1104 90-91 0.5913890096138203 1104 92-93 0.5127865547448636 1104 94-95 0.5795857755679954 1104 96-97 0.5136722116729651 1104 98-99 0.5850396711317849 1104 100 0.8721028602575558 1105 1 0.24451903093003935 1105 2 0.17010789899847367 1105 3 0.20527133478082504 1105 4 0.12622532094055572 1105 5 0.08198861741346519 1105 6 0.11452019916909251 1105 7 0.14663513368132186 1105 8 0.14083618324468006 1105 9 0.15348954062941544 1105 10-11 0.21049720970269448 1105 12-13 0.18772581711569813 1105 14-15 0.28107466398552816 1105 16-17 0.26475235584120327 1105 18-19 0.2745742377998397 1105 20-21 0.2612554738898183 1105 22-23 0.3083132407814375 1105 24-25 0.2744070928932274 1105 26-27 0.28219196815306447 1105 28-29 0.3339220846232891 1105 30-31 0.36983230827993196 1105 32-33 0.4297104100649989 1105 34-35 0.4518597484681024 1105 36-37 0.44971099213482546 1105 38-39 0.46290670871536577 1105 40-41 0.4699756958685626 1105 42-43 0.45680037627201386 1105 44-45 0.5068579855232187 1105 46-47 0.4848496288609354 1105 48-49 0.5288657050089256 1105 50-51 0.6067818883666263 1105 52-53 0.5763269633227637 1105 54-55 0.5158140104527433 1105 56-57 0.554492904185274 1105 58-59 0.5632983325367178 1105 60-61 0.6285463938315345 1105 62-63 0.5690407994856983 1105 64-65 0.6252067277438798 1105 66-67 0.6148884144602604 1105 68-69 0.6484051361414558 1105 70-71 0.7428932197639213 1105 72-73 0.668292439449516 1105 74-75 0.5417271611354302 1105 76-77 0.5727889120955147 1105 78-79 0.7548071681664297 1105 80-81 0.7488344886475744 1105 82-83 0.672295132004809 1105 84-85 0.6714090247521369 1105 86-87 0.663861103749106 1105 88-89 0.733201634790781 1105 90-91 0.7655031628246576 1105 92-93 0.6737050365024757 1105 94-95 0.7514955846574018 1105 96-97 0.8135947919864428 1105 98-99 0.8247354057153196 1105 100 0.8805390355133476 1106 1 0.24894745313732258 1106 2 0.195777326882407 1106 3 0.1938381635928792 1106 4 0.1686246994484506 1106 5 0.17734167366992182 1106 6 0.13422130023469947 1106 7 0.15977923418678586 1106 8 0.16406488208538406 1106 9 0.15631080616608983 1106 10-11 0.1176067045437108 1106 12-13 0.2136772561723106 1106 14-15 0.2694810379918593 1106 16-17 0.3151314876966751 1106 18-19 0.29077046110958804 1106 20-21 0.3130367856029679 1106 22-23 0.25261687695772395 1106 24-25 0.2976139496134351 1106 26-27 0.2283815465218808 1106 28-29 0.3893930391680982 1106 30-31 0.43651406978825236 1106 32-33 0.40061204015739804 1106 34-35 0.3788165370884471 1106 36-37 0.40951747716775344 1106 38-39 0.3987987656983236 1106 40-41 0.44766744625917454 1106 42-43 0.4307079316290512 1106 44-45 0.47959047091674023 1106 46-47 0.5101427090825226 1106 48-49 0.479689299435357 1106 50-51 0.5222421817092169 1106 52-53 0.6049616923344914 1106 54-55 0.5318544995436696 1106 56-57 0.5601778753917017 1106 58-59 0.6487805512747684 1106 60-61 0.6262694455760922 1106 62-63 0.5892226730139001 1106 64-65 0.6983807661011312 1106 66-67 0.7385365382000302 1106 68-69 0.6616639203209402 1106 70-71 0.6795433096958661 1106 72-73 0.7096232647014205 1106 74-75 0.6458895198275876 1106 76-77 0.5652433223126145 1106 78-79 0.6770434501459022 1106 80-81 0.6998285509418665 1106 82-83 0.7507096940472167 1106 84-85 0.6399466491340853 1106 86-87 0.6097551362550391 1106 88-89 0.6506717192627036 1106 90-91 0.7550470979440256 1106 92-93 0.781910896431377 1106 94-95 0.9021515355416234 1106 96-97 0.7938508845785712 1106 98-99 0.8527897032001874 1106 100 0.9814360874892678 1107 1 0.19177158913941028 1107 2 0.17815071392538329 1107 3 0.24500711521572072 1107 4 0.16375719377287368 1107 5 0.18559537956905103 1107 6 0.20127809775726746 1107 7 0.1960271462556662 1107 8 0.20111245291590052 1107 9 0.19799806797724528 1107 10-11 0.1868565353210414 1107 12-13 0.2012201010571104 1107 14-15 0.2666904367248506 1107 16-17 0.2544523353454622 1107 18-19 0.2022269087986146 1107 20-21 0.29732551351038694 1107 22-23 0.34377895526349533 1107 24-25 0.3585475897909447 1107 26-27 0.37598768170359165 1107 28-29 0.39092118290133016 1107 30-31 0.3470166665082246 1107 32-33 0.3110880041368915 1107 34-35 0.3533190228546488 1107 36-37 0.4316612452549222 1107 38-39 0.4089647932431646 1107 40-41 0.4579757658466548 1107 42-43 0.44146055096286574 1107 44-45 0.46898828743795207 1107 46-47 0.46097050615491497 1107 48-49 0.5325050783611758 1107 50-51 0.40343841374040323 1107 52-53 0.49181224954998726 1107 54-55 0.48714697388753336 1107 56-57 0.5514354599670384 1107 58-59 0.7193579850361829 1107 60-61 0.6499348898058557 1107 62-63 0.6032339734952075 1107 64-65 0.6916637668030674 1107 66-67 0.6304104291294195 1107 68-69 0.5752429595594961 1107 70-71 0.6077028630259846 1107 72-73 0.5813602642535471 1107 74-75 0.664097019360927 1107 76-77 0.5497990238789789 1107 78-79 0.6498281216269248 1107 80-81 0.6149594686080562 1107 82-83 0.6374783536924227 1107 84-85 0.5895179513772462 1107 86-87 0.5361044710504714 1107 88-89 0.5619273182220788 1107 90-91 0.3847323069287967 1107 92-93 0.52171783223719 1107 94-95 0.7134230808228104 1107 96-97 0.7178120375533759 1107 98-99 0.7870538340677875 1107 100 0.8920388182645631 1108 1 0.1881924287243315 1108 2 0.16107459416828362 1108 3 0.23132593229296106 1108 4 0.14271767990479134 1108 5 0.09934227039030219 1108 6 0.08839725458994252 1108 7 0.11225625655407612 1108 8 0.09625816967960077 1108 9 0.15568844117913727 1108 10-11 0.17850957265888212 1108 12-13 0.1999014993199708 1108 14-15 0.21491319101550488 1108 16-17 0.25813781137365055 1108 18-19 0.28637385992524145 1108 20-21 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82-83 -0.13226500820569953 2316 84-85 -0.13650587940292525 2316 86-87 -0.17864348898003612 2316 88-89 -0.20527804118755455 2316 90-91 -0.17175953554554724 2316 92-93 -0.01113709281551678 2316 94-95 0.06467349825162572 2316 96-97 0.02186702704238286 2316 98-99 -0.08182304975082033 2316 100 -0.11392769795665103 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 3.0 10 15.0 11 42.0 12 105.0 13 323.0 14 1079.0 15 2942.0 16 7147.0 17 15337.0 18 27905.0 19 42092.0 20 59530.0 21 82203.0 22 102154.0 23 105401.0 24 103862.0 25 112042.0 26 131289.0 27 158831.0 28 192339.0 29 232852.0 30 280475.0 31 338795.0 32 412644.0 33 511383.0 34 659479.0 35 880335.0 36 1235766.0 37 1769004.0 38 1472153.0 39 156544.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.32955530764591 22.9621891362426 14.875022199154039 22.83323335695745 2 13.721066355664602 23.77135847022192 40.785859674988174 21.721715499125306 3 15.795431915671077 29.973656282944077 32.19374425514644 22.037167546238408 4 10.341185183474662 19.97972297225599 41.2533270434961 28.42576480077326 5 10.742459100794793 40.52986042796402 36.78577452990133 11.94190594133985 6 26.696521401116225 42.226173530231634 19.14437320727268 11.932931861379464 7 24.5682909600171 34.38408557295685 24.681804266684026 16.365819200342024 8 24.465989033658207 37.44184082529947 21.466255582796936 16.625914558245388 9 26.26350273430507 14.770338606944911 21.91029904716077 37.05585961158925 10-11 22.797734088343987 28.151399018791107 30.509211039083855 18.54165585378105 12-13 23.927616491065404 26.43697201962418 30.082851628442565 19.552559860867852 14-15 21.477552229536375 27.330189679272955 28.17569502296437 23.016563068226304 16-17 19.85478700785934 30.2004965680198 28.993379276339915 20.951337147780944 18-19 19.66361534876755 29.03845109200644 31.698910291781583 19.599023267444426 20-21 20.775536211606124 28.218260684109136 31.606262627606625 19.39994047667811 22-23 20.363933139859654 28.49943831640952 31.050381302666086 20.086247241064743 24-25 20.140884958593695 28.691380931993553 31.492266980306376 19.67546712910637 26-27 20.088748939488717 28.61508098592266 31.52880601837646 19.76736405621217 28-29 19.922102699299813 28.300373047138095 31.958868597788108 19.818655655773984 30-31 20.352322589250655 28.458136036781305 31.520424474819404 19.669116899148634 32-33 20.2057893102426 28.66380011840951 31.400722509155628 19.729688062192267 34-35 20.361731660427495 28.746027151042902 31.54280834957387 19.349432838955735 36-37 20.525131837171898 28.322144433302764 31.295495054257305 19.857228675268033 38-39 20.145568946904117 28.96848984313511 31.351590297859378 19.534350912101395 40-41 20.271875333335533 28.589455985414062 31.628348888075138 19.510319793175267 42-43 20.14002110065169 28.54867698649967 31.21471815542387 20.09658375742476 44-45 20.29535753388572 28.84916195465416 31.014009108843105 19.84147140261701 46-47 20.377637393192636 28.938490855812628 30.564223748352 20.11964800264274 48-49 20.24779031022945 29.043798674304355 30.52513442557798 20.18327658988821 50-51 20.40315639255158 29.123583769757005 30.097755310736257 20.37550452695515 52-53 20.517673230594472 29.224966439927126 30.019301681068928 20.238058648409474 54-55 20.616352042190872 29.212137573387025 29.952451695695252 20.21905868872685 56-57 20.57236442726417 29.295483677147455 29.87737977740333 20.254772118185045 58-59 20.463319635401145 29.091076037269225 30.13378851375053 20.3118158135791 60-61 20.705793071337432 29.63426847371546 29.64864137585733 20.011297079089783 62-63 20.431008842643745 29.23688819749306 30.196264977872904 20.13583798199029 64-65 20.29160901741035 29.037905664373454 30.086374365968144 20.584110952248054 66-67 20.351204326595546 29.227525774635698 29.690331571274314 20.730938327494446 68-69 20.336801952415158 29.272799694891145 29.829345128881656 20.56105322381204 70-71 20.411696901664193 29.022540549147667 29.983963545575072 20.581799003613067 72-73 20.52077773613018 29.15154573947939 29.65621965722642 20.671456867164007 74-75 20.32383826428831 29.47563908639241 29.42531942292275 20.775203226396535 76-77 20.150542035339843 29.234246974226156 29.84735215098695 20.767858839447047 78-79 20.245675855208017 29.14751069877749 29.62029079076792 20.986522655246574 80-81 20.100073612162216 29.486877922795422 29.42759450981272 20.98545395522964 82-83 20.399568536937483 29.30520831740953 29.498174541281806 20.79704860437118 84-85 20.090044860211883 29.386320219868928 29.418038006354557 21.105596913564632 86-87 19.87805314627293 29.401847481965966 29.73862615834691 20.981473213414198 88-89 20.278512407465115 29.07229784449174 29.567433946154992 21.08175580188815 90-91 20.25140013499544 29.175512658764546 29.629898685229765 20.94318852101025 92-93 20.27740882342055 29.167975369205358 29.403187094681467 21.151428712692628 94-95 19.93394686588609 28.863516827522385 29.54433193347123 21.65820437312029 96-97 20.193807167285232 28.801120950344167 29.482324850133935 21.522747032236673 98-99 19.858451645435697 28.808315285336157 29.468183239159313 21.865049830068838 100 19.000892148957202 29.328913060787986 29.655429461063726 22.01476532919109 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4748.0 1 3263.5 2 2465.0 3 3259.5 4 2717.0 5 2525.0 6 4099.5 7 8174.0 8 12879.0 9 15113.5 10 13379.0 11 11053.5 12 11276.5 13 12173.0 14 13176.5 15 14129.0 16 15298.5 17 16513.5 18 18042.5 19 20088.0 20 22613.5 21 26176.0 22 30838.5 23 37612.5 24 46260.5 25 57876.5 26 75369.5 27 93735.0 28 107311.0 29 127068.5 30 156788.5 31 183273.0 32 215554.0 33 257897.0 34 303386.0 35 338397.5 36 370722.5 37 400235.5 38 407714.5 39 410524.0 40 392875.0 41 365575.5 42 351597.0 43 342726.0 44 334853.0 45 333989.5 46 326807.5 47 308461.5 48 298463.5 49 285268.0 50 269158.5 51 243958.5 52 213617.5 53 192188.0 54 172326.5 55 150537.5 56 128884.0 57 107804.0 58 89738.0 59 74416.5 60 59336.0 61 45751.5 62 35317.5 63 26809.5 64 19641.0 65 14146.0 66 10066.5 67 7529.0 68 5612.0 69 4042.0 70 3027.0 71 2238.5 72 1614.0 73 1212.0 74 933.5 75 651.5 76 444.5 77 314.5 78 215.5 79 149.0 80 107.5 81 77.5 82 57.0 83 44.0 84 33.5 85 23.0 86 15.5 87 12.0 88 8.5 89 6.0 90 7.0 91 6.0 92 5.0 93 5.5 94 5.0 95 5.0 96 5.0 97 3.0 98 3.0 99 4.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0030019502780267797 2 0.057048051437373376 3 8.686962343007897E-4 4 5.827962078220487E-4 5 0.0016054386102267758 6 5.827962078220487E-4 7 0.0 8 8.796923891653567E-4 9 4.3984619458267834E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.367423759259863E-4 18-19 0.001033638557269294 20-21 0.0019078328690023674 22-23 0.0 24-25 1.6494232296850438E-5 26-27 8.247116148425219E-5 28-29 6.927577564677184E-4 30-31 0.001330534738612602 32-33 0.0024631386896629987 34-35 0.0036342291827393797 36-37 0.0011545962607795307 38-39 0.0012700558868574837 40-41 0.004376469636097649 42-43 0.005827962078220488 44-45 0.004370971558665366 46-47 0.005465088967689778 48-49 0.007405910301285846 50-51 0.0073564276043952955 52-53 0.00352426763409371 54-55 0.0019573155658929183 56-57 0.0021607444308874074 58-59 0.0028370079550582754 60-61 0.006454742905500804 62-63 0.011540464530363023 64-65 0.0033208387690992214 66-67 0.008236119993560652 68-69 0.010336385572692942 70-71 0.004750338901492926 72-73 0.006515221757255923 74-75 0.002501625231688983 76-77 0.002375169450746463 78-79 0.007065029500484271 80-81 0.005300146644721274 82-83 0.01434998209825988 84-85 0.015372624500664607 86-87 0.01942470756825753 88-89 0.01801719974559296 90-91 0.021211582733749665 92-93 0.029739100831221336 94-95 0.033796681976246544 96-97 0.019826067220814224 98-99 0.015784980308085866 100 0.015790478385518152 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9094088.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.236083382828056 #Duplication Level Percentage of deduplicated Percentage of total 1 68.1753744447517 30.158115485935603 2 16.38864311535399 14.499387667644204 3 6.652469421944043 8.82837576152492 4 3.1747415252713176 5.617525233233151 5 1.796872431495881 3.9743299353978396 6 1.089816087539134 2.892551719219715 7 0.678025029660183 2.0995220223384625 8 0.4564792754181353 1.6154284231943656 9 0.31791405756043645 1.2656945482934998 >10 1.131312972276643 7.920672943880741 >50 0.05458514516391019 1.7137455062282319 >100 0.06325581751890902 6.378137884152414 >500 0.012514224425254284 3.8637102802987893 >1k 0.007680207771138926 6.303458935950326 >5k 1.80710771085575E-4 0.47294128225699383 >10k+ 1.3553307831418125E-4 2.3964023704508084 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 46997 0.5167862901700533 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 40669 0.4472026221870737 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32245 0.35457101360796156 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 1.0996154864566959E-5 3 0.0 0.0 0.0 0.0 1.0996154864566959E-5 4 0.0 0.0 0.0 0.0 1.0996154864566959E-5 5 0.0 0.0 0.0 0.0 1.0996154864566959E-5 6 0.0 0.0 0.0 0.0 1.0996154864566959E-5 7 0.0 0.0 0.0 0.0 1.0996154864566959E-5 8 0.0 0.0 0.0 0.0 1.0996154864566959E-5 9 0.0 0.0 0.0 0.0 1.0996154864566959E-5 10-11 0.0 0.0 0.0 0.0 1.0996154864566959E-4 12-13 0.0 0.0 0.0 0.0 1.4295001323937046E-4 14-15 0.0 0.0 0.0 0.0 1.4295001323937046E-4 16-17 0.0 0.0 0.0 1.0996154864566959E-5 1.7044040040078786E-4 18-19 0.0 0.0 0.0 1.0996154864566959E-5 2.474134844527566E-4 20-21 0.0 0.0 0.0 8.247116148425219E-5 3.0789233620787484E-4 22-23 0.0 0.0 0.0 1.5394616810393742E-4 3.1339041364015835E-4 24-25 0.0 0.0 0.0 3.0239425877559133E-4 3.188884910724418E-4 26-27 0.0 0.0 0.0 5.003250463377966E-4 3.4637887823385917E-4 28-29 0.0 0.0 0.0 0.0013690212806385864 3.90363497692127E-4 30-31 0.0 0.0 0.0 0.004931775456758281 4.0685772998897747E-4 32-33 0.0 0.0 0.0 0.012772033875194523 4.0685772998897747E-4 34-35 0.0 0.0 0.0 0.024719356135546523 4.178538848535444E-4 36-37 0.0 0.0 0.0 0.044798334918245794 4.178538848535444E-4 38-39 0.0 0.0 0.0 0.07708304560061438 4.3434811715039484E-4 40-41 0.0 0.0 0.0 0.11577301649159322 4.508423494472453E-4 42-43 0.0 0.0 0.0 0.15274208914626736 5.333135109314975E-4 44-45 0.0 0.0 0.0 0.1998056319666139 5.498077432283478E-4 46-47 0.0 0.0 0.0 0.25887147782163533 5.608038980929148E-4 48-49 0.0 0.0 0.0 0.32560164361725996 6.322789047126001E-4 50-51 0.0 0.0 0.0 0.39964425239782153 6.487731370094505E-4 52-53 0.0 0.0 0.0 0.4716525725284383 6.487731370094505E-4 54-55 0.0 0.0 0.0 0.5512592356704707 6.487731370094505E-4 56-57 0.0 0.0 0.0 0.6468323156758544 6.487731370094505E-4 58-59 0.0 0.0 0.0 0.7585697433321517 6.65267369306301E-4 60-61 0.0 0.0 0.0 0.8849870377326456 6.707654467385844E-4 62-63 0.0 0.0 0.0 1.0142908227850884 6.707654467385844E-4 64-65 0.0 0.0 0.0 1.1451120772088417 7.367423759259862E-4 66-67 0.0 0.0 0.0 1.2955724642207114 7.367423759259862E-4 68-69 0.0 0.0 0.0 1.4728799633344214 7.367423759259862E-4 70-71 0.0 0.0 0.0 1.6774689226671218 7.477385307905532E-4 72-73 0.0 0.0 0.0 1.8884026633566775 7.477385307905532E-4 74-75 5.498077432283479E-6 0.0 0.0 2.096675334569008 8.577000794362228E-4 76-77 1.0996154864566959E-5 0.0 0.0 2.3220965092926305 9.731597055141759E-4 78-79 1.0996154864566959E-5 0.0 0.0 2.583178214242044 9.786577829464594E-4 80-81 1.0996154864566959E-5 0.0 0.0 2.8803822879215595 0.0010061481701078767 82-83 1.0996154864566959E-5 1.0996154864566959E-5 0.0 3.1951637151520855 0.001033638557269294 84-85 1.0996154864566959E-5 1.0996154864566959E-5 0.0 3.4996637375842417 0.001055630866998428 86-87 1.0996154864566959E-5 1.0996154864566959E-5 0.0 3.809969729784889 0.001055630866998428 88 1.0996154864566959E-5 1.0996154864566959E-5 0.0 4.060879991484578 0.001055630866998428 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 21510 0.0 47.635155 1 GTATCAA 37615 0.0 36.97838 1 GTACATG 43565 0.0 29.046028 1 ATCAACG 48160 0.0 28.239386 3 TCAACGC 48435 0.0 28.146818 4 CAACGCA 48890 0.0 27.894325 5 TACATGG 44895 0.0 27.818192 2 ACATGGG 44085 0.0 27.16138 3 AACGCAG 50490 0.0 27.111408 6 TATCAAC 50910 0.0 26.886724 2 ACGCAGA 57045 0.0 23.823067 7 CATGGGA 24690 0.0 23.639668 4 CGCAGAG 57470 0.0 23.55695 8 TACCTGG 5485 0.0 22.717896 2 CATGGGG 14880 0.0 22.707369 4 GCAGAGT 62240 0.0 21.313671 9 AGAGTAC 57230 0.0 21.147583 10-11 GAGTACT 36535 0.0 20.695951 12-13 AGTACTT 40200 0.0 20.346348 12-13 ATGGGAT 11980 0.0 19.574118 5 >>END_MODULE