FastQCFastQC Report
Mon 6 Jun 2016
ERR1211538_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211538_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1402223
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30260.2158001972582107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26700.19041193875724477No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20360.14519801771900762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21300.038.9016151
GTATCAA36600.034.859631
GTACCTG14250.034.0296061
TACCTGG13700.033.9762232
GTACATG56450.027.9392951
TCAACGC44200.027.6366944
ATCAACG44200.027.531383
CAACGCA44500.027.4493985
TACATGG56700.027.115972
ACATGGG55250.026.532173
AACGCAG46400.026.4257016
TATCAAC47400.026.2862132
CATGGGG28350.025.1898444
ACCTGGG17450.024.7709623
ACGCAGA53250.022.3213467
CGCAGAG54200.021.5833848
ATGGGGG18350.021.2501265
GCAGAGT61100.018.9935539
GTCGAAA1250.00634936118.8319171
AGAGTAC55500.018.70729610-11
Produced by FastQC (version 0.11.5)