FastQCFastQC Report
Mon 6 Jun 2016
ERR1211538_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211538_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1402223
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38890.27734532952319285No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37760.26928669690912216No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28220.2012518693531628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26500.038.8614461
TACCTGG13800.038.501142
GTACCTG14200.037.751761
GTATCAA41300.031.539181
ACCTGGG18350.028.4258373
TCAACGC49200.025.500994
CAACGCA50300.024.9433145
ATCAACG50250.024.8755053
GTACATG55100.024.6641961
AACGCAG51800.024.492296
TATCAAC51700.024.4644782
TACATGG56050.023.7402362
ACATGGG55000.022.7271673
ACGCAGA58800.021.576547
CATGGGG25650.021.4343034
CGCAGAG59950.021.0842658
GGTATAC2258.014649E-820.885453
TAACGCA1656.870768E-519.93544
TACCGTA1200.005071592519.5787127
GCAGAGT66750.018.6547749
Produced by FastQC (version 0.11.5)