##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211538_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1402223 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12780563433919 33.0 31.0 34.0 30.0 34.0 2 32.38088092978079 34.0 31.0 34.0 31.0 34.0 3 32.4001432011884 34.0 31.0 34.0 31.0 34.0 4 36.025701332812254 37.0 35.0 37.0 35.0 37.0 5 35.95203330711306 37.0 35.0 37.0 35.0 37.0 6 35.97130912843392 37.0 35.0 37.0 35.0 37.0 7 35.89564926548773 37.0 35.0 37.0 35.0 37.0 8 35.89629324294353 37.0 35.0 37.0 35.0 37.0 9 37.55784208360582 39.0 37.0 39.0 35.0 39.0 10-11 37.52255490032613 39.0 37.0 39.0 35.0 39.0 12-13 37.44407950803831 39.0 37.0 39.0 34.0 39.0 14-15 38.69197410112372 40.0 38.0 41.0 34.0 41.0 16-17 38.62666351928331 40.0 38.0 41.0 34.0 41.0 18-19 38.60781131103968 40.0 38.0 41.0 34.0 41.0 20-21 38.54523638536809 40.0 38.0 41.0 34.0 41.0 22-23 38.43310800065325 40.0 38.0 41.0 34.0 41.0 24-25 38.329206909314706 40.0 38.0 41.0 34.0 41.0 26-27 38.162238459931125 40.0 38.0 41.0 33.5 41.0 28-29 38.19858253644392 40.0 38.0 41.0 34.0 41.0 30-31 38.12972508652333 40.0 38.0 41.0 33.5 41.0 32-33 37.96162236677048 40.0 37.0 41.0 33.0 41.0 34-35 37.85305047770576 40.0 37.0 41.0 33.0 41.0 36-37 37.7934729354746 40.0 37.0 41.0 33.0 41.0 38-39 37.70268495096714 40.0 37.0 41.0 33.0 41.0 40-41 37.54666483148543 40.0 37.0 41.0 32.0 41.0 42-43 37.40756641418662 40.0 37.0 41.0 31.5 41.0 44-45 37.228610213924604 40.0 36.0 41.0 31.0 41.0 46-47 36.98730979309282 39.0 36.0 41.0 30.5 41.0 48-49 36.778478173585796 39.0 36.0 41.0 30.0 41.0 50-51 36.59009515604865 39.0 35.0 40.5 30.0 41.0 52-53 36.39024891190631 39.0 35.0 40.0 29.5 41.0 54-55 36.1946730298961 39.0 35.0 40.0 29.0 41.0 56-57 35.99499615966933 38.5 35.0 40.0 28.0 41.0 58-59 35.737528196299735 38.0 34.0 40.0 28.0 41.0 60-61 35.41943150269251 38.0 34.0 40.0 27.5 41.0 62-63 35.096148044925805 37.5 34.0 40.0 26.5 41.0 64-65 34.77933074839024 37.0 33.5 40.0 26.0 41.0 66-67 34.43139571951109 36.5 33.0 39.0 26.0 41.0 68-69 34.03212149565368 36.0 32.5 39.0 26.0 40.0 70-71 33.64276866090486 35.5 32.0 38.5 25.0 40.0 72-73 33.22490573895878 35.0 32.0 38.0 24.5 40.0 74-75 32.81368690999933 35.0 31.0 37.0 24.0 39.0 76-77 31.32280814107314 33.5 29.5 35.5 22.0 38.5 78-79 32.09207629599572 34.0 31.0 36.0 23.5 39.0 80-81 32.02173762661146 34.0 31.0 36.0 23.5 37.5 82-83 31.72352935303443 34.0 31.0 35.5 22.5 37.0 84-85 31.420143586291196 34.0 30.5 35.0 21.5 37.0 86-87 31.11537394551366 34.0 30.0 35.0 20.5 36.0 88-89 30.830371488700443 34.0 30.0 35.0 20.0 36.0 90-91 30.55307215756695 34.0 29.5 35.0 20.0 35.5 92-93 30.333215900751878 34.0 29.0 35.0 19.0 35.0 94-95 30.097298361244967 34.0 29.0 35.0 18.5 35.0 96-97 29.897795857007054 34.0 29.0 35.0 18.0 35.0 98-99 29.726472536821888 34.0 29.0 35.0 18.0 35.0 100 27.72804539648829 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3631372503485011 1101 2 0.29810601412631854 1101 3 0.33761589469280295 1101 4 0.2775613752125139 1101 5 0.20784094664451658 1101 6 0.11871173737154805 1101 7 0.2767651675037328 1101 8 0.1244130544219928 1101 9 0.17388870779079468 1101 10-11 0.21098555898706195 1101 12-13 0.2554129332861166 1101 14-15 0.23680094443028565 1101 16-17 0.36357057516144664 1101 18-19 0.3351737030643491 1101 20-21 0.29079336254012134 1101 22-23 0.3421898218617727 1101 24-25 0.3344581822918471 1101 26-27 0.0998125549192892 1101 28-29 0.282419308977893 1101 30-31 0.4411113752085356 1101 32-33 0.42934684316383454 1101 34-35 0.44407221150260767 1101 36-37 0.4206940628037259 1101 38-39 0.5789959909896538 1101 40-41 0.5037930727884898 1101 42-43 0.3413721507483771 1101 44-45 0.43282815065328606 1101 46-47 0.482829228244519 1101 48-49 0.5806472145093764 1101 50-51 0.6225166236990916 1101 52-53 0.6948123291476094 1101 54-55 0.5941865621560538 1101 56-57 0.6536805921159967 1101 58-59 0.46872575119786575 1101 60-61 0.4715240203975739 1101 62-63 0.6799668079704233 1101 64-65 0.6685095980308802 1101 66-67 0.5161682527591083 1101 68-69 0.5316330152587909 1101 70-71 0.661776475269761 1101 72-73 0.4665774125836677 1101 74-75 0.5781769981973852 1101 76-77 0.5380560447302862 1101 78-79 0.6932514965987977 1101 80-81 0.7556018048307109 1101 82-83 0.5084066386519943 1101 84-85 0.6562475886852326 1101 86-87 0.7308563352796362 1101 88-89 0.7838490020597355 1101 90-91 0.6702530747700486 1101 92-93 0.6408047753085739 1101 94-95 0.7443300807736861 1101 96-97 0.6658643980727099 1101 98-99 0.45639337200370633 1101 100 0.723694140723925 1102 1 0.2740655713135425 1102 2 0.23929884822000957 1102 3 0.30804469070299234 1102 4 0.1650470026002182 1102 5 0.1627615869996717 1102 6 0.15053976246763057 1102 7 0.2039762672974561 1102 8 0.2854034308346485 1102 9 0.37612527171646803 1102 10-11 0.2638589401958171 1102 12-13 0.27240685327881664 1102 14-15 0.3917377049362045 1102 16-17 0.34964875646606686 1102 18-19 0.438103005504054 1102 20-21 0.4179428348548342 1102 22-23 0.42234334846573063 1102 24-25 0.38847423967387584 1102 26-27 0.3078659254573566 1102 28-29 0.48432990348334926 1102 30-31 0.5434650598755582 1102 32-33 0.571075779262415 1102 34-35 0.6291450945559021 1102 36-37 0.6086350487863257 1102 38-39 0.5928828589156865 1102 40-41 0.5113702569262628 1102 42-43 0.41268741239412066 1102 44-45 0.4128256113729023 1102 46-47 0.4162565720145821 1102 48-49 0.6706033619930665 1102 50-51 0.650291072832168 1102 52-53 0.41033375182937704 1102 54-55 0.37415149726174945 1102 56-57 0.3696421091791038 1102 58-59 0.46200735739257226 1102 60-61 0.3978328197173937 1102 62-63 0.3508717903809142 1102 64-65 0.43680097617538394 1102 66-67 0.5797986248350142 1102 68-69 0.5241785154542313 1102 70-71 0.4833556508881003 1102 72-73 0.49613007126729514 1102 74-75 0.26949964943435845 1102 76-77 0.4299711471717025 1102 78-79 0.42021252007145193 1102 80-81 0.3598435443233683 1102 82-83 0.3487094407050968 1102 84-85 0.3146644472244873 1102 86-87 0.3492417238608603 1102 88-89 0.1560868213599811 1102 90-91 0.37505459719702827 1102 92-93 0.423826832810434 1102 94-95 0.6303827266182545 1102 96-97 0.42656432645929954 1102 98-99 0.3614435441897399 1102 100 0.4027562047309097 1103 1 0.315409079004894 1103 2 0.20038406137649645 1103 3 0.29460457313952304 1103 4 0.09434025501173693 1103 5 0.1923380851632288 1103 6 0.16323686884376087 1103 7 0.20576182060086978 1103 8 0.2335206026810681 1103 9 0.17586752002082306 1103 10-11 0.1942584698644083 1103 12-13 0.2728328851337878 1103 14-15 0.4731543796000537 1103 16-17 0.4432725281711498 1103 18-19 0.32311705631574483 1103 20-21 0.37402738400262336 1103 22-23 0.3154781771272539 1103 24-25 0.30435693987249834 1103 26-27 0.27686520134860615 1103 28-29 0.212806328266673 1103 30-31 0.27062073744069437 1103 32-33 0.39698045475604005 1103 34-35 0.3610043285903757 1103 36-37 0.47130181432728335 1103 38-39 0.4585483264892787 1103 40-41 0.4985972984621867 1103 42-43 0.5695003354573203 1103 44-45 0.34922472615722455 1103 46-47 0.49441669601457505 1103 48-49 0.5296454640216339 1103 50-51 0.5296423896534961 1103 52-53 0.43493915133480954 1103 54-55 0.37441588904432166 1103 56-57 0.4656015435794103 1103 58-59 0.5363450693058596 1103 60-61 0.5200666418968396 1103 62-63 0.5398595697128812 1103 64-65 0.579813423672654 1103 66-67 0.598788304104275 1103 68-69 0.6465029723969025 1103 70-71 0.6374209350595947 1103 72-73 0.5704854739231351 1103 74-75 0.5633479724004289 1103 76-77 0.5507039219622492 1103 78-79 0.5456304335719651 1103 80-81 0.7040334197902354 1103 82-83 0.754552038656076 1103 84-85 0.5602422462634706 1103 86-87 0.47848398815958504 1103 88-89 0.6808245379261741 1103 90-91 0.7794501893425299 1103 92-93 0.6209693174437199 1103 94-95 0.5777195670807131 1103 96-97 0.6165546619824092 1103 98-99 0.6565740824826847 1103 100 0.7224986856806446 1104 1 0.22748522677738947 1104 2 0.26750727528634144 1104 3 0.2886971977314019 1104 4 0.13922710537421779 1104 5 0.19165718035773693 1104 6 0.18172507416274897 1104 7 0.20850404110438348 1104 8 0.27015435813295596 1104 9 0.2780967367600127 1104 10-11 0.2777946224340724 1104 12-13 0.33550451866896225 1104 14-15 0.4673591013885172 1104 16-17 0.3950212933887087 1104 18-19 0.39089718916805083 1104 20-21 0.3428832093855405 1104 22-23 0.44971187775431787 1104 24-25 0.5159636500400566 1104 26-27 0.5442573995795286 1104 28-29 0.5350189507047105 1104 30-31 0.44950510110554376 1104 32-33 0.5991568046950917 1104 34-35 0.5344480621612817 1104 36-37 0.5194343435631055 1104 38-39 0.47091059134216096 1104 40-41 0.6909636751772936 1104 42-43 0.6483440700072478 1104 44-45 0.44107491730986226 1104 46-47 0.655574772642062 1104 48-49 0.7913728377654365 1104 50-51 0.7580272110077644 1104 52-53 0.7281910469522543 1104 54-55 0.8262128707416494 1104 56-57 0.7709161255156047 1104 58-59 0.7086729707386397 1104 60-61 0.7643871321874585 1104 62-63 0.6884409772424931 1104 64-65 0.7143981011117475 1104 66-67 0.7327919362492423 1104 68-69 0.7250013594699922 1104 70-71 0.607053608700113 1104 72-73 0.6765250163316097 1104 74-75 0.7373984504044131 1104 76-77 0.7756521565537859 1104 78-79 0.8076551830665437 1104 80-81 1.0446970449842041 1104 82-83 0.8241271951760716 1104 84-85 0.8991284700564677 1104 86-87 0.9477703353084515 1104 88-89 0.927839521747952 1104 90-91 0.7814627990533829 1104 92-93 0.7662054313423035 1104 94-95 0.7567378110218357 1104 96-97 0.7193056936192725 1104 98-99 0.8286692231426365 1104 100 1.139864940018903 1105 1 0.20001915524110814 1105 2 0.17724368882153385 1105 3 0.19735513849983732 1105 4 0.0844421364376089 1105 5 0.07300346179810902 1105 6 0.12188793078860272 1105 7 0.14672468167377417 1105 8 0.2079117620776998 1105 9 0.18691874049968504 1105 10-11 0.14398196787107054 1105 12-13 0.22182456209738888 1105 14-15 0.2981836384534873 1105 16-17 0.20050051408959746 1105 18-19 0.14067703113681063 1105 20-21 0.13952595020224123 1105 22-23 0.17704766398991012 1105 24-25 0.13526035068544928 1105 26-27 0.3000556986325478 1105 28-29 0.4105180761043101 1105 30-31 0.41875656379930604 1105 32-33 0.373234855240959 1105 34-35 0.3787703976459298 1105 36-37 0.46409807478936216 1105 38-39 0.5267704140093912 1105 40-41 0.5094617751833326 1105 42-43 0.41886340169190817 1105 44-45 0.565529950785951 1105 46-47 0.4661085804712428 1105 48-49 0.5979088170162967 1105 50-51 0.6506455836478509 1105 52-53 0.7216164646493368 1105 54-55 0.6107040945790274 1105 56-57 0.5274997554088472 1105 58-59 0.6099661729824462 1105 60-61 0.6910292698314677 1105 62-63 0.4724725834913599 1105 64-65 0.3519902357398905 1105 66-67 0.3663597027007839 1105 68-69 0.3651111368992659 1105 70-71 0.49441834722475164 1105 72-73 0.4535405178696976 1105 74-75 0.43316659082301356 1105 76-77 0.40676621650914413 1105 78-79 0.4395835261511678 1105 80-81 0.6029780028224323 1105 82-83 0.6230499287546998 1105 84-85 0.570808305993495 1105 86-87 0.5650174551588627 1105 88-89 0.7972731425935997 1105 90-91 0.7597908043612982 1105 92-93 0.6799843082347579 1105 94-95 0.8330162355347746 1105 96-97 0.9718480665006268 1105 98-99 0.7551328216708946 1105 100 0.9630605489217317 1106 1 0.18942228056739197 1106 2 0.13327658943522636 1106 3 0.18932441888592422 1106 4 0.009558205069495784 1106 5 0.08008196618911256 1106 6 -0.007191988932042648 1106 7 0.08276848728636566 1106 8 0.1343252935571897 1106 9 0.10863059330539926 1106 10-11 0.15589254688011067 1106 12-13 0.1855097556611227 1106 14-15 0.044205750078909034 1106 16-17 0.27898123892398985 1106 18-19 0.25704381033379065 1106 20-21 0.162211364186156 1106 22-23 0.20204331359141747 1106 24-25 0.24927652256679522 1106 26-27 0.24873514967026011 1106 28-29 0.24329339832382857 1106 30-31 0.3912003276480789 1106 32-33 0.2936209386062032 1106 34-35 0.40405816200514266 1106 36-37 0.4392175077294169 1106 38-39 0.33491784031363636 1106 40-41 0.23656789010345136 1106 42-43 0.12022142208657982 1106 44-45 0.2574041024582385 1106 46-47 0.4376623107929376 1106 48-49 0.19658327959707123 1106 50-51 0.19607627309900977 1106 52-53 0.2876311515946597 1106 54-55 0.43374171682523155 1106 56-57 0.3799980442521047 1106 58-59 0.49335878774595443 1106 60-61 0.3882322536247784 1106 62-63 0.3362556900239184 1106 64-65 0.4305916116259212 1106 66-67 0.2407385238008004 1106 68-69 0.17478201475167765 1106 70-71 0.43586291154683465 1106 72-73 0.29293726261151676 1106 74-75 0.34192028866173274 1106 76-77 0.49633291681889347 1106 78-79 0.472867670978836 1106 80-81 0.5513852929302061 1106 82-83 0.4114159190794062 1106 84-85 0.2729013697181344 1106 86-87 0.09031078802815173 1106 88-89 0.4025360806787326 1106 90-91 0.5885997232582731 1106 92-93 0.49321369604668064 1106 94-95 0.5050667233594588 1106 96-97 0.5053426546048989 1106 98-99 0.38994714666306507 1106 100 0.696450031398328 1107 1 0.20360920250209347 1107 2 0.23308271097721445 1107 3 0.30295740881480526 1107 4 0.1986255716297265 1107 5 0.08880762478705861 1107 6 0.11381758677778464 1107 7 0.06387695589921805 1107 8 0.10872223770422096 1107 9 0.12372622181316473 1107 10-11 0.13033393372696622 1107 12-13 0.11613425004080824 1107 14-15 0.13845066860888977 1107 16-17 0.26577340997017984 1107 18-19 0.05660610613988837 1107 20-21 0.23614416411685824 1107 22-23 0.30522437315050155 1107 24-25 0.21223274267231318 1107 26-27 0.30396998083538307 1107 28-29 0.3165469242993453 1107 30-31 0.2613926765553032 1107 32-33 0.30826965854364374 1107 34-35 0.41875967174847517 1107 36-37 0.5119237342644638 1107 38-39 0.4703365514572937 1107 40-41 0.3895500403811454 1107 42-43 0.41915858732814826 1107 44-45 0.36283752706830086 1107 46-47 0.34526480987137376 1107 48-49 0.4631097600014442 1107 50-51 0.38490879270229783 1107 52-53 0.47353316017909464 1107 54-55 0.644406205122813 1107 56-57 0.4266428008933545 1107 58-59 0.4224844125945211 1107 60-61 0.4228416113172244 1107 62-63 0.45502936929742077 1107 64-65 0.6028077502366145 1107 66-67 0.7273337482635043 1107 68-69 0.7116586269275373 1107 70-71 0.675465462963885 1107 72-73 0.5372083278872353 1107 74-75 0.4805842192823988 1107 76-77 0.5754736341376052 1107 78-79 0.6825733700670114 1107 80-81 0.6113042041788077 1107 82-83 0.5703748228923224 1107 84-85 0.5290984191740549 1107 86-87 0.5535733694857576 1107 88-89 0.5880658534524521 1107 90-91 0.5638979292621435 1107 92-93 0.5778851607201503 1107 94-95 0.7459382604948281 1107 96-97 0.6222720746344343 1107 98-99 0.9542816770165281 1107 100 0.9845199413443346 1108 1 0.2287301895768863 1108 2 0.21168131571583615 1108 3 0.17137346736500092 1108 4 0.15121888098886416 1108 5 0.10749807194516592 1108 6 0.16183722915425847 1108 7 0.14378623031705473 1108 8 0.21000847656692656 1108 9 0.11920782955284182 1108 10-11 0.2100475434804494 1108 12-13 0.2646539825059193 1108 14-15 0.20166387939841712 1108 16-17 0.31169592850815775 1108 18-19 0.3286118369114135 1108 20-21 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-0.5694863267209485 2316 90-91 -0.5737404868386804 2316 92-93 -0.3309201612579571 2316 94-95 -0.4002625406105089 2316 96-97 -0.28468870021394466 2316 98-99 -0.5622032989091998 2316 100 -0.7128260449756212 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 3.0 11 4.0 12 13.0 13 33.0 14 104.0 15 292.0 16 722.0 17 1406.0 18 2535.0 19 3946.0 20 5462.0 21 7670.0 22 10050.0 23 11844.0 24 13113.0 25 14365.0 26 16776.0 27 20637.0 28 24840.0 29 30583.0 30 37215.0 31 45753.0 32 56191.0 33 71622.0 34 94620.0 35 130545.0 36 189465.0 37 288556.0 38 280200.0 39 43656.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.412856965580396 21.654426080792145 14.026798538832065 22.905918414795394 2 15.524972740921728 22.162415582487967 39.92591524852858 22.38669642806172 3 17.44914463718753 27.39906661760133 31.055860312135398 24.095928433075738 4 11.948487067923445 17.506566415468143 38.57079997232941 31.974146544278998 5 12.287938564586204 38.96531706162084 35.63844505872251 13.108299315070445 6 30.555799217666333 38.38263033842884 18.068127926173947 12.993442517730877 7 26.923820248277195 33.025702759118914 22.986999927971514 17.063477064632373 8 25.904536410380757 36.86323731823336 20.384678471840882 16.847547799545005 9 26.88081710255787 14.872099516268097 20.257976085116276 37.98910729605776 10-11 24.6585600150618 26.6184123352705 29.429841045254573 19.293186604413133 12-13 25.016955220389335 24.480093394559923 29.32668341626118 21.17626796878956 14-15 22.399183296807998 25.920342199493234 26.62900266220138 25.051471841497392 16-17 21.956503832684778 28.83572460866393 27.41818215531065 21.789589403340642 18-19 21.919410873466664 28.023625537231723 29.12770291785512 20.9292606714465 20-21 22.588294529526124 27.25288158027988 29.119508023922712 21.039315866271284 22-23 22.410772038399028 27.131490497588473 28.840134557770053 21.617602906242446 24-25 22.180609710655762 27.29905560636775 28.913100453030815 21.607234229945675 26-27 22.085870976023735 27.39755530515896 28.91176134985951 21.604812368957795 28-29 22.087945708405186 27.390447623245084 29.064809307619566 21.456797360730164 30-31 22.39682470630102 27.29601533588979 28.969598509771423 21.337561448037764 32-33 22.204056220546118 27.37274923833541 28.980054086744182 21.443140454374294 34-35 22.2629684148067 27.51156060294856 28.937736774841387 21.28773420740335 36-37 22.370812951831073 27.440770326291837 28.79452521767409 21.393891504203005 38-39 22.28066184236707 27.551459948495477 28.78173479379293 21.386143415344524 40-41 22.314195170534678 27.443375897318678 28.924622556499298 21.317806375647347 42-43 22.181681379165248 27.402689793014666 28.726012327475082 21.689616500345004 44-45 22.167524749368294 27.632319347976008 28.631101937942756 21.56905396471294 46-47 22.165745281071043 27.61842476404909 28.421444136860263 21.7943858180196 48-49 22.129054413304907 27.662138197648652 28.445161449067292 21.763645939979153 50-51 22.267114079600038 27.70168350408484 28.326159749239554 21.705042667075567 52-53 22.263424041124637 27.77239527263508 28.184041024792528 21.780139661447755 54-55 22.232765189807292 27.693760904750647 28.345416144718456 21.72805776072361 56-57 22.279585260242886 27.810256953366558 28.047601574832914 21.862556211557642 58-59 22.216132446325975 27.764362045061645 28.26401686531143 21.755488643300946 60-61 22.386161753829672 27.89505064747319 28.21060334074342 21.50818425795372 62-63 22.24624602778084 27.870883397291813 28.25277988476419 21.63009069016316 64-65 22.1552946680507 27.656837088261526 28.221814682071734 21.966053561616043 66-67 22.134913519809032 27.708679685588823 28.151083400553308 22.00532339404884 68-69 22.07596906633421 27.799895442326743 28.217020648357426 21.90711484298162 70-71 22.182311576424947 27.615999600616192 28.187653334854794 22.014035488104067 72-73 22.10063039309171 27.908141905842566 27.915559134209754 22.07566856685597 74-75 22.085253842385015 27.906330744286258 27.897166501805394 22.11124891152334 76-77 21.92407632519116 27.80629082692042 28.107356770810117 22.162276077078303 78-79 21.97409777629431 27.78901077583286 28.009213198023847 22.227678249848978 80-81 22.0603616277349 27.96340574089824 27.744738817479803 22.231493813887063 82-83 22.107047421102425 27.99714127473612 27.76786551723277 22.12794578692868 84-85 21.936227046265884 28.004786002902986 27.747975221201067 22.311011729630064 86-87 21.858118385777527 27.920529291155425 27.92516568880285 22.296186634264192 88-89 21.79372507566015 27.809803615281957 27.93070293503605 22.465768374021838 90-91 22.00406653829597 27.901046379293682 27.848012328722664 22.246874753687685 92-93 21.981309304579295 27.865550546592804 27.721737554843862 22.431402593984036 94-95 21.729394244745453 27.81569631089432 27.777387396224395 22.677522048135828 96-97 21.732996137175732 27.71275937468682 27.88755119196179 22.66669329617566 98-99 21.720897406132657 27.670069974112327 27.805767184955123 22.803265434799897 100 20.50639014780293 28.442612778013153 28.073711735075985 22.977285339107933 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 522.0 1 374.5 2 457.0 3 648.5 4 459.0 5 328.5 6 497.5 7 1031.0 8 1634.5 9 1857.5 10 1555.0 11 1210.5 12 1209.0 13 1318.5 14 1385.5 15 1428.0 16 1538.0 17 1637.0 18 1724.5 19 1872.0 20 2103.0 21 2423.5 22 2892.0 23 3511.0 24 4297.5 25 5425.5 26 6845.0 27 8607.5 28 10660.0 29 13255.5 30 16559.0 31 19442.0 32 22643.0 33 26613.5 34 30685.5 35 34676.5 36 39329.5 37 43191.5 38 46010.5 39 48403.0 40 49055.0 41 49344.5 42 50337.0 43 51471.5 44 52094.0 45 53490.5 46 54501.0 47 54205.5 48 54804.5 49 54958.0 50 54233.5 51 52157.5 52 48259.5 53 45008.0 54 42089.0 55 38426.5 56 34206.0 57 29870.5 58 25846.5 59 21940.5 60 17906.5 61 14192.0 62 11140.0 63 8476.5 64 6250.5 65 4611.0 66 3354.5 67 2479.0 68 1897.0 69 1473.0 70 1075.5 71 791.0 72 596.5 73 452.0 74 356.0 75 257.0 76 186.5 77 120.5 78 77.5 79 57.5 80 44.5 81 31.0 82 29.0 83 26.5 84 12.0 85 6.5 86 6.5 87 5.0 88 3.5 89 3.0 90 2.0 91 0.0 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00406497397346927 2 0.06097460960203905 3 7.844686615467013E-4 4 8.557839944145832E-4 5 0.0024960366503758674 6 5.705226629430554E-4 7 0.0 8 9.27099327282465E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 5.348649965091145E-4 18-19 8.201263279806422E-4 20-21 0.002923928647583159 22-23 0.0 24-25 3.5657666433940965E-5 26-27 2.139459986036458E-4 28-29 0.0012123606587539928 30-31 0.0015689373230934026 32-33 0.00221077531890434 34-35 0.0033518206447904508 36-37 8.557839944145832E-4 38-39 0.0011410453258861109 40-41 0.0042432623056389744 42-43 0.005027730967185676 44-45 0.004706811969280207 46-47 0.00552693829726085 48-49 0.00666798362314696 50-51 0.00627574929237361 52-53 0.0033161629783565096 54-55 0.0017472256552631073 56-57 0.003030901646884982 58-59 0.003922343307733506 60-61 0.005705226629430554 62-63 0.010091119600805292 64-65 0.003102216979752864 66-67 0.007844686615467012 68-69 0.008985731941353122 70-71 0.004492865970676561 72-73 0.005847857295166318 74-75 0.0024247213175079855 76-77 0.002745640315413454 78-79 0.00741679461825972 80-81 0.006454037624543314 82-83 0.014227408907142445 84-85 0.01554674256519826 86-87 0.019433428206497825 88-89 0.016402526559612843 90-91 0.020931050196723346 92-93 0.028704421479322475 94-95 0.03284071078565963 96-97 0.019219482207894177 98-99 0.014583985571481854 100 0.015760688563801906 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1402223.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.32073358396697 #Duplication Level Percentage of deduplicated Percentage of total 1 83.66592850084047 62.18113182166193 2 11.313972566833035 16.81725481831818 3 2.6265190056798517 5.856144578230742 4 0.9482926604039238 2.8191122469404513 5 0.45133515896008125 1.6771780053074787 6 0.26728130941945316 1.191872579360221 7 0.15677327765987753 0.8156053501431526 8 0.10871727037834868 0.6463957830212284 9 0.08587886092820796 0.5744321949185904 >10 0.3477000432278113 4.419281093808569 >50 0.019075332335649092 0.9646352468118571 >100 0.007714031963649394 0.9823915643858703 >500 5.074883560370867E-4 0.2634903116452999 >1k 3.0449301362225206E-4 0.7910744054463956 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3889 0.27734532952319285 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3776 0.26928669690912216 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2822 0.2012518693531628 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 1.4263066573576386E-4 0.0 0.0 0.0 5 0.0 1.4263066573576386E-4 0.0 0.0 0.0 6 0.0 1.4263066573576386E-4 0.0 0.0 0.0 7 0.0 1.4263066573576386E-4 0.0 0.0 0.0 8 0.0 1.4263066573576386E-4 0.0 0.0 0.0 9 0.0 1.4263066573576386E-4 0.0 0.0 7.131533286788193E-5 10-11 0.0 1.4263066573576386E-4 0.0 0.0 7.131533286788193E-5 12-13 0.0 1.4263066573576386E-4 0.0 0.0 7.131533286788193E-5 14-15 0.0 1.4263066573576386E-4 0.0 0.0 1.069729993018229E-4 16-17 0.0 1.4263066573576386E-4 0.0 7.131533286788193E-5 1.4263066573576386E-4 18-19 0.0 1.4263066573576386E-4 0.0 7.131533286788193E-5 1.4263066573576386E-4 20-21 0.0 1.4263066573576386E-4 0.0 1.4263066573576386E-4 2.852613314715277E-4 22-23 0.0 1.4263066573576386E-4 0.0 5.348649965091145E-4 3.2091899790546866E-4 24-25 0.0 1.4263066573576386E-4 0.0 8.557839944145832E-4 3.5657666433940965E-4 26-27 0.0 1.4263066573576386E-4 0.0 9.98414660150347E-4 3.5657666433940965E-4 28-29 0.0 1.4263066573576386E-4 0.0 0.0017828833216970482 3.9223433077335064E-4 30-31 0.0 1.4263066573576386E-4 0.0 0.004100631639903211 4.278919972072916E-4 32-33 0.0 1.4263066573576386E-4 0.0 0.008843101275617358 4.278919972072916E-4 34-35 0.0 1.4263066573576386E-4 0.0 0.01675910322395225 4.278919972072916E-4 36-37 0.0 1.4263066573576386E-4 0.0 0.03048730480101952 4.278919972072916E-4 38-39 0.0 1.4263066573576386E-4 0.0 0.0517036163292144 4.278919972072916E-4 40-41 0.0 1.4263066573576386E-4 0.0 0.07744845149451977 4.278919972072916E-4 42-43 0.0 1.4263066573576386E-4 0.0 0.10230184499897663 4.278919972072916E-4 44-45 0.0 1.4263066573576386E-4 0.0 0.13200468113844943 4.635496636412325E-4 46-47 0.0 1.4263066573576386E-4 0.0 0.16841115856750316 4.992073300751735E-4 48-49 0.0 1.4263066573576386E-4 0.0 0.2089182676364601 4.992073300751735E-4 50-51 0.0 1.4263066573576386E-4 0.0 0.25213535935439657 4.992073300751735E-4 52-53 0.0 1.4263066573576386E-4 0.0 0.2946036400772203 4.992073300751735E-4 54-55 0.0 1.4263066573576386E-4 0.0 0.3432050394266818 4.992073300751735E-4 56-57 0.0 1.4263066573576386E-4 0.0 0.40581990168468207 4.992073300751735E-4 58-59 0.0 1.4263066573576386E-4 0.0 0.4781693068791483 4.992073300751735E-4 60-61 0.0 1.4263066573576386E-4 0.0 0.5521589647295758 4.992073300751735E-4 62-63 0.0 1.4263066573576386E-4 0.0 0.6270400642408518 4.992073300751735E-4 64-65 0.0 1.4263066573576386E-4 0.0 0.7084465167095391 4.992073300751735E-4 66-67 0.0 1.4263066573576386E-4 0.0 0.8044012970832741 4.992073300751735E-4 68-69 0.0 1.4263066573576386E-4 0.0 0.9187910910033568 4.992073300751735E-4 70-71 0.0 1.4263066573576386E-4 0.0 1.051544583136919 4.992073300751735E-4 72-73 0.0 1.4263066573576386E-4 0.0 1.1799121822991063 4.992073300751735E-4 74-75 0.0 1.4263066573576386E-4 0.0 1.3121664671025934 4.992073300751735E-4 76-77 0.0 1.4263066573576386E-4 0.0 1.4626418194538244 4.992073300751735E-4 78-79 0.0 1.4263066573576386E-4 0.0 1.634904005996193 4.992073300751735E-4 80-81 0.0 1.4263066573576386E-4 0.0 1.8224276737722889 5.348649965091145E-4 82-83 0.0 1.7828833216970483E-4 0.0 2.02927779675558 5.705226629430554E-4 84-85 0.0 2.139459986036458E-4 0.0 2.2294955937821586 5.705226629430554E-4 86-87 0.0 2.139459986036458E-4 0.0 2.4369518970948274 5.705226629430554E-4 88 0.0 2.139459986036458E-4 0.0 2.6126372196148546 5.705226629430554E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2650 0.0 38.861446 1 TACCTGG 1380 0.0 38.50114 2 GTACCTG 1420 0.0 37.75176 1 GTATCAA 4130 0.0 31.53918 1 ACCTGGG 1835 0.0 28.425837 3 TCAACGC 4920 0.0 25.50099 4 CAACGCA 5030 0.0 24.943314 5 ATCAACG 5025 0.0 24.875505 3 GTACATG 5510 0.0 24.664196 1 AACGCAG 5180 0.0 24.49229 6 TATCAAC 5170 0.0 24.464478 2 TACATGG 5605 0.0 23.740236 2 ACATGGG 5500 0.0 22.727167 3 ACGCAGA 5880 0.0 21.57654 7 CATGGGG 2565 0.0 21.434303 4 CGCAGAG 5995 0.0 21.084265 8 GGTATAC 225 8.014649E-8 20.88545 3 TAACGCA 165 6.870768E-5 19.9354 4 TACCGTA 120 0.0050715925 19.578712 7 GCAGAGT 6675 0.0 18.654774 9 >>END_MODULE