FastQCFastQC Report
Mon 6 Jun 2016
ERR1211537_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211537_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250177
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6090.24342765322151916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4040.16148566814695195No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3290.1315068931196713No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3250.1299080251182163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG6400.070.5546342
ACCTGGG6600.066.92433
GTACCTG7300.062.563051
CCTGGGG6100.048.530044
TGGGGGG5600.043.633026
GGTATCA4150.043.1126371
CTGGGGG4350.042.1284335
CATGGGG6150.041.2589874
ATGGGGG4350.039.9685
ATAGGGC608.815989E-539.1578373
TGCACCG500.001701365837.5915225
CTAATAG500.001701365837.5915223
GGATAGG651.3956682E-436.21811
GTACATG11100.036.054961
TACATGG11400.035.0710142
ACATGGG11700.032.9327433
GTATACA1052.0285133E-631.3890251
GTCTAAA753.234402E-431.3890231
TACAGCG600.004159403531.326277
GTATCAA6050.029.5435372
Produced by FastQC (version 0.11.5)