##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211537_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 250177 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.913749065661513 31.0 31.0 34.0 27.0 34.0 2 30.889678107899606 31.0 31.0 34.0 27.0 34.0 3 30.98848415321952 31.0 31.0 34.0 27.0 34.0 4 34.48603588659229 37.0 35.0 37.0 30.0 37.0 5 34.56246977140185 37.0 35.0 37.0 30.0 37.0 6 34.69380078904136 37.0 35.0 37.0 31.0 37.0 7 34.63881571847132 37.0 35.0 37.0 30.0 37.0 8 34.60979626424491 37.0 35.0 37.0 30.0 37.0 9 36.15837187271412 39.0 37.0 39.0 30.0 39.0 10-11 36.1004428864364 39.0 37.0 39.0 30.0 39.0 12-13 36.01540309460901 39.0 36.0 39.0 30.0 39.0 14-15 37.17997257941377 40.0 37.0 41.0 30.5 41.0 16-17 37.13425095032717 40.0 37.0 41.0 30.0 41.0 18-19 37.13191660304504 40.0 37.0 41.0 30.0 41.0 20-21 37.0690111401128 40.0 37.0 41.0 30.0 41.0 22-23 36.89283986937248 40.0 36.0 41.0 30.0 41.0 24-25 36.803319249971025 40.0 36.0 41.0 30.0 41.0 26-27 36.65189046155322 40.0 36.0 41.0 29.0 41.0 28-29 36.471070482098675 39.5 36.0 41.0 28.5 41.0 30-31 36.26760653457352 39.0 35.0 41.0 27.5 41.0 32-33 36.04375901861482 39.0 35.0 41.0 27.0 41.0 34-35 35.81262865890949 39.0 35.0 41.0 26.0 41.0 36-37 35.61909967742838 39.0 35.0 40.0 25.0 41.0 38-39 35.470660772173304 39.0 35.0 40.0 25.0 41.0 40-41 35.424027788325866 39.0 35.0 40.0 25.0 41.0 42-43 35.364050252421286 39.0 34.5 40.0 24.0 41.0 44-45 35.199374842611434 38.0 34.0 40.0 24.0 41.0 46-47 35.139733068987155 38.5 34.0 40.0 23.5 41.0 48-49 35.114582875324274 39.0 34.0 40.0 23.0 41.0 50-51 34.17391486827326 38.0 33.0 39.5 22.0 40.5 52-53 34.37848003613442 38.0 33.5 39.5 22.5 40.5 54-55 34.94205302645727 38.5 34.0 40.0 23.0 41.0 56-57 34.89689100117117 38.0 34.0 40.0 23.0 41.0 58-59 34.73109238659029 38.0 34.0 40.0 22.5 41.0 60-61 34.54097099253728 38.0 33.5 40.0 22.0 41.0 62-63 34.25354049333072 37.5 33.0 40.0 22.0 41.0 64-65 33.963401911446695 37.0 33.0 40.0 21.5 41.0 66-67 33.675070050404315 36.5 32.5 39.5 21.0 41.0 68-69 33.37071753198735 36.0 32.0 39.0 20.0 41.0 70-71 33.0405532882719 35.5 32.0 39.0 20.0 41.0 72-73 32.71005128369114 35.0 32.0 38.5 20.0 40.0 74-75 32.35767476626548 35.0 31.0 37.0 19.5 39.5 76-77 32.025747770578434 35.0 31.0 37.0 19.0 39.0 78-79 31.711028591757035 35.0 31.0 36.0 18.5 39.0 80-81 31.395076285989518 35.0 31.0 36.0 18.0 38.5 82-83 31.113605567258382 35.0 30.0 35.5 18.0 37.0 84-85 30.88052458859128 34.5 30.0 35.0 18.0 37.0 86-87 30.594135352170664 34.0 30.0 35.0 17.0 36.0 88-89 30.399247332888315 34.0 30.0 35.0 16.5 36.0 90-91 30.172465894146946 34.0 29.0 35.0 15.0 36.0 92-93 30.01251513928139 34.0 29.0 35.0 15.0 35.5 94-95 29.848213464866873 34.0 29.0 35.0 13.0 35.0 96-97 29.74730890529505 34.0 29.0 35.0 13.0 35.0 98-99 29.575060856913304 34.0 29.0 35.0 10.5 35.0 100 28.219716440759942 32.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1573643685722672 1101 2 0.23352193357672135 1101 3 -0.13803749489277095 1101 4 0.13438226298332268 1101 5 0.4487351975818541 1101 6 0.14078423145103613 1101 7 -0.07283920482719708 1101 8 0.17952659772023338 1101 9 -0.020309998748309965 1101 10-11 -0.05751251423331638 1101 12-13 -0.18637552330699947 1101 14-15 -0.42648101230650326 1101 16-17 -0.23716260648015464 1101 18-19 -0.2463118979301413 1101 20-21 -0.3842527778109712 1101 22-23 -0.013709667755271937 1101 24-25 -0.3252307073872913 1101 26-27 -0.16126526763314075 1101 28-29 -0.1004415570509849 1101 30-31 -0.08969008868572104 1101 32-33 -0.39623510953734353 1101 34-35 -0.46951334950831836 1101 36-37 -0.6570743364227738 1101 38-39 -0.4576672876135248 1101 40-41 -0.6617289861732303 1101 42-43 -0.04796224374744895 1101 44-45 -0.6328305261654847 1101 46-47 -0.9427462660672532 1101 48-49 -0.700732247417335 1101 50-51 -0.4917397719401748 1101 52-53 -0.8387122139495986 1101 54-55 -0.45860913214587384 1101 56-57 -0.5551674141483502 1101 58-59 -0.6083144159177394 1101 60-61 -0.6903325564804135 1101 62-63 -0.7233549367346583 1101 64-65 -0.5421516589899653 1101 66-67 -1.1222943526520552 1101 68-69 -0.8249633515252981 1101 70-71 -0.49761455727581705 1101 72-73 -0.6653390637670924 1101 74-75 -0.5191703176014641 1101 76-77 -0.5445833915664835 1101 78-79 -0.9067290729758248 1101 80-81 -0.7975350962475289 1101 82-83 -0.7641660452025185 1101 84-85 -0.9050300096497033 1101 86-87 -0.5101698408187332 1101 88-89 -0.2621599993763688 1101 90-91 -0.22236265795963917 1101 92-93 -0.41025534323621926 1101 94-95 -0.9550029692044788 1101 96-97 -0.7593711142204036 1101 98-99 -0.9116536347194462 1101 100 -0.9583150477587807 1102 1 0.27020676788940534 1102 2 0.02518795359689463 1102 3 0.36167539406509164 1102 4 0.3362756439098362 1102 5 0.3720222310264063 1102 6 -0.052635493853536275 1102 7 0.16830303153502513 1102 8 0.23856283578960813 1102 9 0.09388259844266145 1102 10-11 0.01512657942696194 1102 12-13 0.36810573687768766 1102 14-15 0.20605565254955138 1102 16-17 0.29504038878638994 1102 18-19 0.3354604307732032 1102 20-21 0.2517757780980787 1102 22-23 0.45357519295707505 1102 24-25 0.4569761677581994 1102 26-27 0.4023414678367061 1102 28-29 0.4085287230762731 1102 30-31 0.013569009630408857 1102 32-33 -0.24192456402636964 1102 34-35 -0.116715956786976 1102 36-37 0.1731964791277818 1102 38-39 0.19093207608631957 1102 40-41 0.011515368602900367 1102 42-43 0.09693571543622426 1102 44-45 -0.022727321385481503 1102 46-47 -0.04139606819342845 1102 48-49 0.4330459787020047 1102 50-51 0.5347210024836642 1102 52-53 0.24329290749061272 1102 54-55 0.11190378785102695 1102 56-57 0.2421865084092687 1102 58-59 0.4645807497530896 1102 60-61 -0.07956977227927808 1102 62-63 0.38532047259256785 1102 64-65 0.44409029026121516 1102 66-67 0.33146525004834615 1102 68-69 -0.05751553589713865 1102 70-71 0.03051456509556516 1102 72-73 -0.08481605608493226 1102 74-75 -0.10914471040040752 1102 76-77 0.027271966546344117 1102 78-79 -0.045155394074605226 1102 80-81 -0.12457785251353215 1102 82-83 0.2844177989815435 1102 84-85 0.2665770362104638 1102 86-87 0.2794553628749128 1102 88-89 0.12087716986394881 1102 90-91 9.942133059759328E-4 1102 92-93 -0.05210371756693988 1102 94-95 -0.0380401384447957 1102 96-97 0.0045537713231667 1102 98-99 0.2966182671972142 1102 100 -0.3472496329997199 1103 1 -0.495440812540771 1103 2 -0.31641405557908797 1103 3 -0.235815942064999 1103 4 -0.2021189795801277 1103 5 -0.4320690798486737 1103 6 -0.3054240673666442 1103 7 -0.5572732736576782 1103 8 -0.4806435958184352 1103 9 -0.5322310771427681 1103 10-11 -0.5206723907300486 1103 12-13 -0.5827382012194917 1103 14-15 -0.39854216148941646 1103 16-17 -0.6149395475148793 1103 18-19 -0.8213100152582555 1103 20-21 -0.9086145520409588 1103 22-23 -0.6280405661662982 1103 24-25 -0.6247939275092946 1103 26-27 -0.331428683553014 1103 28-29 -0.1965180688365109 1103 30-31 -0.36212024155867795 1103 32-33 -0.7041122087165093 1103 34-35 -0.1287120843528129 1103 36-37 -0.6668341074898692 1103 38-39 -0.4533371422712591 1103 40-41 -0.2479026438281764 1103 42-43 -0.3643188971099036 1103 44-45 -0.5026776532081882 1103 46-47 -0.5752938976660147 1103 48-49 -0.8861001215042421 1103 50-51 -0.5446428519913766 1103 52-53 -0.45520441968798053 1103 54-55 -0.19063714630356543 1103 56-57 -0.5378844862170311 1103 58-59 -0.5791104095919692 1103 60-61 -0.43358882577779667 1103 62-63 -0.18212442238870352 1103 64-65 -0.3271204066366309 1103 66-67 -0.38596631590230146 1103 68-69 -0.38752830370543023 1103 70-71 -0.24703092899055434 1103 72-73 -0.3402844662823412 1103 74-75 -0.19941732415316693 1103 76-77 -0.2035938592221811 1103 78-79 -0.22767191505730722 1103 80-81 -0.2600849870525579 1103 82-83 -0.2612516691199822 1103 84-85 -0.12101012863456972 1103 86-87 -0.33705439537856563 1103 88-89 -0.36051830949008234 1103 90-91 -0.1443974497361289 1103 92-93 -0.4057369307051566 1103 94-95 -0.6857959506036799 1103 96-97 -0.7735739909430492 1103 98-99 -0.6703327521228637 1103 100 -0.9680597574507743 1104 1 0.5393405253716175 1104 2 0.4381558663577536 1104 3 0.3134880658265651 1104 4 0.45995645305539057 1104 5 0.5154422764530082 1104 6 0.7806037236043366 1104 7 0.5539271452771999 1104 8 0.44145156572724176 1104 9 0.7130772175243365 1104 10-11 0.635563528064786 1104 12-13 0.5037706341658961 1104 14-15 0.5831842399330043 1104 16-17 0.6959551950959195 1104 18-19 0.7433021655170151 1104 20-21 0.37940317962150516 1104 22-23 0.35427039555718665 1104 24-25 0.6342089100438386 1104 26-27 0.9278907908298493 1104 28-29 0.5691280529712373 1104 30-31 0.4671698830257398 1104 32-33 0.29845742094018846 1104 34-35 0.5078558159796529 1104 36-37 0.45952834425346367 1104 38-39 0.33454662765554843 1104 40-41 0.7243490093814273 1104 42-43 0.6414901708817737 1104 44-45 0.41181822442386107 1104 46-47 0.3598199089175935 1104 48-49 -0.14432354532263503 1104 50-51 0.12205426052430113 1104 52-53 0.14440221994276925 1104 54-55 0.5774385542554228 1104 56-57 0.4897238096679146 1104 58-59 0.46391566423313435 1104 60-61 0.48084007098641024 1104 62-63 0.5721593085674144 1104 64-65 0.5744846985233352 1104 66-67 0.32116897327237837 1104 68-69 0.4270808324849398 1104 70-71 0.10617745457842886 1104 72-73 0.11432686200916464 1104 74-75 0.06636884856004599 1104 76-77 0.06250895032076897 1104 78-79 0.02312881077173401 1104 80-81 0.13577803098356256 1104 82-83 0.35246357694336794 1104 84-85 0.14221859276155513 1104 86-87 0.477901823490555 1104 88-89 0.2964848446998758 1104 90-91 0.45477076966884056 1104 92-93 0.30046062631992143 1104 94-95 0.007474298930500112 1104 96-97 -0.11050602362971063 1104 98-99 -0.0836412416434058 1104 100 -0.10037741726103633 1105 1 0.4772773337583409 1105 2 0.5968022457015714 1105 3 0.48930344148177696 1105 4 0.12756953789328662 1105 5 0.19960554572110567 1105 6 -0.04599548043371726 1105 7 0.5158762813672837 1105 8 0.4085332003612905 1105 9 0.7997230144569727 1105 10-11 0.844715877123221 1105 12-13 0.6417232161888009 1105 14-15 0.5653782313909801 1105 16-17 0.8095435759928336 1105 18-19 0.7307439243987872 1105 20-21 0.7634062858144759 1105 22-23 0.9921501761823066 1105 24-25 0.9829700729248287 1105 26-27 0.6690393542155988 1105 28-29 1.0945539411482983 1105 30-31 0.9506985667764596 1105 32-33 0.8128660309188334 1105 34-35 1.024709339567309 1105 36-37 0.33296918098419326 1105 38-39 0.6144740689290913 1105 40-41 1.1538772563237671 1105 42-43 0.9939945389656373 1105 44-45 1.0332934234143423 1105 46-47 0.788256134893139 1105 48-49 0.6440306577447217 1105 50-51 0.9859763144944012 1105 52-53 0.5780194787274766 1105 54-55 1.072888466893744 1105 56-57 0.883458036031719 1105 58-59 1.0213074328217502 1105 60-61 1.102667143399536 1105 62-63 1.0399628903961897 1105 64-65 0.9663867563761812 1105 66-67 0.7012650711174544 1105 68-69 0.5739637193030376 1105 70-71 0.8694697764576702 1105 72-73 0.5663386073013399 1105 74-75 1.0773557928427167 1105 76-77 0.9934568245199458 1105 78-79 0.7592298105775939 1105 80-81 0.59394149438873 1105 82-83 0.6031733905717935 1105 84-85 0.8374294741438142 1105 86-87 1.0937167138031114 1105 88-89 0.787870012628435 1105 90-91 0.6677633924605324 1105 92-93 0.42261530502308275 1105 94-95 0.6240111364377796 1105 96-97 0.3054022951433417 1105 98-99 0.5485054289060045 1105 100 0.45368975416198865 1106 1 0.2697057916279739 1106 2 0.1257171900576921 1106 3 0.45949422049608657 1106 4 0.5711307461603781 1106 5 0.3935642102680106 1106 6 0.38415157838981173 1106 7 0.4441354228617129 1106 8 0.5240936137158201 1106 9 0.5734710271755716 1106 10-11 0.4645986275206795 1106 12-13 0.40657814464666586 1106 14-15 0.5158452425197524 1106 16-17 0.3073078844189041 1106 18-19 0.3204696795657256 1106 20-21 0.2256479392326014 1106 22-23 0.5705981094096515 1106 24-25 0.5586358121935149 1106 26-27 0.34993164356376383 1106 28-29 0.4852037821657831 1106 30-31 0.4644604904648588 1106 32-33 0.48153653966286925 1106 34-35 0.4313071138121387 1106 36-37 0.5262819790764013 1106 38-39 0.6230690609799652 1106 40-41 0.3706896569596978 1106 42-43 0.3604492289497401 1106 44-45 0.31523897190400874 1106 46-47 0.5197744178820045 1106 48-49 0.297647509890993 1106 50-51 0.5979596213306522 1106 52-53 0.870547300667063 1106 54-55 0.7585999136346047 1106 56-57 0.9557950789570029 1106 58-59 0.8983940782907567 1106 60-61 0.5733050607286359 1106 62-63 0.48744897942639653 1106 64-65 0.8446657706846068 1106 66-67 0.7886060886055262 1106 68-69 0.5639776302728308 1106 70-71 0.5526474394147485 1106 72-73 0.3540969538832144 1106 74-75 0.5879380662926295 1106 76-77 0.5749353618808399 1106 78-79 0.9004758360147918 1106 80-81 0.3881585055175023 1106 82-83 0.4789186210964367 1106 84-85 0.522196962220125 1106 86-87 0.6789710858221909 1106 88-89 0.4943485499893221 1106 90-91 0.7650408169438343 1106 92-93 0.47026037656521424 1106 94-95 0.19152902197037136 1106 96-97 0.17757041914970273 1106 98-99 0.3891254607025054 1106 100 0.48429388770729886 1107 1 0.12887767977910158 1107 2 0.2860323495077992 1107 3 0.34613735151637925 1107 4 0.2724657316649086 1107 5 0.20695848689757668 1107 6 -0.1110906361430608 1107 7 -0.15540399544932626 1107 8 -0.0813024397771187 1107 9 -0.3445243903873134 1107 10-11 0.1261821400989973 1107 12-13 0.1264766763964147 1107 14-15 0.258392937273328 1107 16-17 0.012290115785219768 1107 18-19 0.020752574917821676 1107 20-21 -0.2833065169536866 1107 22-23 -0.2112451525374226 1107 24-25 -0.13997542880527192 1107 26-27 0.06404367947511247 1107 28-29 0.02870186502372718 1107 30-31 0.1432807467072621 1107 32-33 0.04026654994997614 1107 34-35 0.13651729804482216 1107 36-37 -0.052519573341065495 1107 38-39 -0.08135205563838355 1107 40-41 0.15560736012999854 1107 42-43 -0.09337570324889555 1107 44-45 -0.02477318558219821 1107 46-47 -0.11010026649095295 1107 48-49 -0.07807141837983522 1107 50-51 -0.30446486197618583 1107 52-53 0.03325304274131469 1107 54-55 -0.04800253656646447 1107 56-57 -0.1368706836914626 1107 58-59 0.03385068541691538 1107 60-61 -0.01487178393610833 1107 62-63 0.10970770008477615 1107 64-65 0.37187983596096075 1107 66-67 0.13335849672299105 1107 68-69 -0.16100600388035957 1107 70-71 -0.19551724776378165 1107 72-73 0.09164137546743234 1107 74-75 -0.2666104701330667 1107 76-77 -0.11099819278108569 1107 78-79 -0.38728835551236784 1107 80-81 -0.4302799588984705 1107 82-83 -0.3158361249991408 1107 84-85 -0.46523056045826294 1107 86-87 -0.1828836322176457 1107 88-89 0.09626101608864346 1107 90-91 0.003836056820393452 1107 92-93 -0.3430637607208631 1107 94-95 -0.19508488432346738 1107 96-97 -0.2885639678324381 1107 98-99 0.19621304136275342 1107 100 0.09036795485402749 1108 1 -0.1651892682670848 1108 2 0.2139510282915289 1108 3 0.2640556500575144 1108 4 0.015282315311942796 1108 5 0.009802701506501421 1108 6 0.17123949047772413 1108 7 -0.23568588195958995 1108 8 0.1556647452869555 1108 9 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0.08633894081636129 2316 64-65 0.5139707899896209 2316 66-67 0.4945423820418213 2316 68-69 0.311516240311434 2316 70-71 0.3608038100711255 2316 72-73 0.14690583419209702 2316 74-75 0.3078704987250589 2316 76-77 0.7245492614947366 2316 78-79 0.3774546004935644 2316 80-81 0.636903679262673 2316 82-83 0.8391502813280951 2316 84-85 0.42634754137070274 2316 86-87 0.6916158734669793 2316 88-89 0.5136053067460828 2316 90-91 0.8937087706117914 2316 92-93 0.5596426159474532 2316 94-95 0.821017438958787 2316 96-97 0.6990472531265368 2316 98-99 0.9518667734438182 2316 100 0.237093115506525 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 1.0 1 0.0 2 4.0 3 6.0 4 2.0 5 6.0 6 17.0 7 169.0 8 426.0 9 725.0 10 986.0 11 1260.0 12 1269.0 13 1312.0 14 1227.0 15 1195.0 16 1260.0 17 1526.0 18 1875.0 19 2124.0 20 2208.0 21 2439.0 22 2606.0 23 2662.0 24 2695.0 25 2754.0 26 2807.0 27 3175.0 28 3646.0 29 4148.0 30 5102.0 31 6255.0 32 7956.0 33 10404.0 34 14754.0 35 19853.0 36 30839.0 37 48682.0 38 49463.0 39 12314.0 40 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.191563211856504 16.67861017586984 9.55654217393219 23.573284438341467 2 17.169562938392037 20.10091269571341 39.07345980097552 23.65606456491903 3 18.47071618206826 27.296244762178933 30.22182771966862 24.01121133608419 4 11.65498462608807 14.582625280388967 36.96336250844666 36.79902758507631 5 11.506091295464813 38.08948826720936 35.051637073139524 15.352783364186301 6 30.351275512243483 35.231878612485716 20.394205632879515 14.022640242391295 7 28.672047072555536 31.897892208005054 22.362262008978004 17.067798710461414 8 28.864895547685098 33.48829958187095 20.88526634740688 16.76153852303707 9 29.356859327718325 11.973793086907335 19.732734257264266 38.93661332811007 10-11 25.916258097269164 24.48778089354277 30.336802425633042 19.25915858355502 12-13 24.968719393975736 22.881613399692192 30.02478463352721 22.12488257280486 14-15 22.375767364782877 24.43333446609802 27.15396870770873 26.036929461410374 16-17 22.414413406034633 26.650769919570187 28.136842610211225 22.797974064183947 18-19 22.22635339082321 26.393969559950587 29.08515937648672 22.294517672739484 20-21 23.16643377891856 26.11361323841908 28.85169348374084 21.868259498921518 22-23 22.68483805113501 25.683876295420966 29.062018247365167 22.569267406078854 24-25 22.246386951046436 25.664741039838486 29.053311211945548 23.03556079716953 26-27 21.996893907141015 25.784168520528393 29.12191914164703 23.097018430683562 28-29 22.29112317210911 26.5257329055344 28.73189600887583 22.451247913480664 30-31 23.401775826846887 25.960573605664095 28.791422300579285 21.846228266909733 32-33 22.990272708009325 25.933648114312675 28.88701868295745 22.189060494720554 34-35 23.025993575071116 26.414373929261814 28.543356275700216 22.01627621996686 36-37 22.697995754021086 26.452608558326236 28.617178223165773 22.232217464486904 38-39 22.665203389525573 26.838341859121595 28.014360905712575 22.482093845640254 40-41 22.75392479663826 26.373154051141807 28.424663695955953 22.44825745626398 42-43 22.320145071546104 26.79605011926017 27.936269286058458 22.947535523135272 44-45 22.13564940608649 27.11008743254618 28.172553818101115 22.581709343266215 46-47 22.10174695796626 26.265540009715316 28.048080825042334 23.584632207276094 48-49 22.27501414188256 26.370063583503068 28.29415915264638 23.06076312196799 50-51 22.33391433665678 26.66085663343221 28.32051394807749 22.684715081833517 52-53 22.318799439461213 26.931250037482535 28.067120721743667 22.682829801312586 54-55 22.212959575544215 26.530485769176178 28.53361641047785 22.722938244801753 56-57 22.487291278108614 26.88511875133684 27.723704489981948 22.903885480572598 58-59 22.437099437463267 26.92999676151561 27.95371766013506 22.679186140886067 60-61 22.91586699932828 26.992371173591785 28.079718836963824 22.01204299011611 62-63 22.722464511458913 27.012483727961513 27.932916209249914 22.332135551329657 64-65 22.354352433124074 26.691191171178374 27.78979567355752 23.164660722140027 66-67 22.371240774354504 26.798750989532937 27.568187815351152 23.2618204207614 68-69 21.975029738402025 26.893711452533513 28.051260008596646 23.07999880046782 70-71 21.9855759521891 26.531730305896183 28.45160426043264 23.031089481482073 72-73 21.74897979177955 27.574713631901275 27.593308313822245 23.082998262496925 74-75 22.338925912818144 27.271073147420555 27.48781809420407 22.902182845557228 76-77 22.06360375993059 26.90045619744833 27.612340120986286 23.423599921634793 78-79 21.990876780566985 26.865922256737466 28.009323114687906 23.133877848007643 80-81 22.21475892896411 27.002994646419822 27.256679980648745 23.525566443967328 82-83 22.489955022488754 27.161019490254873 27.496851574212894 22.852173913043476 84-85 22.413241644010874 27.533983687829846 27.07840236686391 22.97437230129538 86-87 22.096328606325688 27.543648524925736 27.36873458258537 22.991288286163208 88-89 21.78957447531549 26.972751787595477 27.72077328871591 23.516900448373125 90-91 22.70894351203031 26.654604704165376 27.581443237137513 23.055008546666798 92-93 22.22839744345841 27.008818349203427 27.610769141575386 23.152015065762775 94-95 22.190149305645786 26.671317683793472 27.447331229047933 23.69120178151281 96-97 22.02284339044847 26.764625926488577 27.73109243697479 23.48143824608816 98-99 22.051109831766137 26.705926565598414 27.439550949913645 23.803412652721807 100 22.00507867954332 26.968188271049527 27.443664647191728 23.583068402215424 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2.0 1 3.5 2 28.5 3 71.5 4 78.5 5 72.5 6 97.0 7 151.0 8 202.0 9 251.0 10 282.5 11 293.0 12 307.5 13 313.5 14 339.0 15 382.5 16 424.5 17 448.0 18 476.0 19 495.0 20 525.5 21 570.0 22 644.5 23 788.5 24 947.5 25 1166.5 26 1449.5 27 1711.5 28 1992.0 29 2317.0 30 2756.0 31 3181.0 32 3580.0 33 4043.5 34 4525.0 35 5011.0 36 5618.5 37 6202.0 38 6485.5 39 6737.5 40 6982.5 41 7209.0 42 7420.5 43 7613.0 44 7925.0 45 8338.5 46 8744.5 47 9031.5 48 9392.0 49 9792.5 50 9999.5 51 9903.0 52 9545.5 53 9217.5 54 8733.5 55 8029.0 56 7321.0 57 6664.0 58 6086.5 59 5330.0 60 4393.5 61 3539.5 62 2881.5 63 2278.5 64 1698.5 65 1339.5 66 1075.5 67 888.0 68 742.5 69 578.0 70 465.0 71 379.5 72 320.5 73 276.0 74 228.5 75 178.0 76 147.5 77 114.5 78 87.5 79 70.5 80 47.0 81 39.0 82 36.5 83 28.5 84 24.5 85 18.0 86 11.5 87 12.0 88 10.5 89 6.5 90 4.0 91 2.5 92 1.0 93 0.5 94 1.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15588963014185955 2 0.10312698609384555 3 0.029179341026553198 4 0.031177926028371914 5 0.026381322024007007 6 0.0011991510010912274 7 0.003997170003637425 8 0.005995755005456137 9 0.005596038005092395 10-11 0.006195613505638009 12-13 0.008793774008002334 14-15 0.020585425518732738 16-17 0.008394057007638591 18-19 0.018386982016732153 20-21 0.022584010520551447 22-23 0.04536787954128477 24-25 0.016788114015277182 26-27 0.009393349508547949 28-29 0.023783161521642678 30-31 0.0159886800145497 32-33 0.02238415202036958 34-35 0.022983727520915192 36-37 0.023183586021097064 38-39 0.021384859519460224 40-41 0.027380614524916358 42-43 0.03737353953400992 44-45 0.03937212453582863 46-47 0.022584010520551447 48-49 0.013390519512185374 50-51 0.013990095012730987 52-53 0.02418287852200642 54-55 0.027380614524916358 56-57 0.021384859519460224 58-59 0.023983020021824546 60-61 0.029179341026553198 62-63 0.05256278554783214 64-65 0.0347753790316456 66-67 0.023583303021460805 68-69 0.03177750152891753 70-71 0.04336929453946606 72-73 0.04217014353837483 74-75 0.04536787954128477 76-77 0.026381322024007007 78-79 0.018386982016732153 80-81 0.025581888023279518 82-83 0.020785284018914607 84-85 0.022783869020733323 86-87 0.021584718019642093 88-89 0.020185708518368994 90-91 0.03177750152891753 92-93 0.02937919952673507 94-95 0.02098514251909648 96-97 0.015588963014185957 98-99 0.019586133017823384 100 0.04476830404073916 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 250177.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.88149698276993 #Duplication Level Percentage of deduplicated Percentage of total 1 80.20897969123799 60.061684707404915 2 14.490787570727198 21.701837315107372 3 3.2793413842185455 7.366859759035008 4 0.9047640560614477 2.7100034773633594 5 0.4237516914326753 1.586558050172977 6 0.20062486958798698 0.9013854340032873 7 0.10599348139337791 0.5555865390006055 8 0.07652980186546418 0.458453290198459 9 0.06544088697947704 0.4410280422813148 >10 0.23073126569481708 2.789714036346849 >50 0.008703533867374252 0.47454356028690137 >100 0.0038077960669762336 0.7042796701597015 >500 5.439708667108905E-4 0.24806611863924707 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 609 0.24342765322151916 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 404 0.16148566814695195 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 329 0.1315068931196713 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 325 0.1299080251182163 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 3.9971700036374246E-4 16-17 0.0 0.0 0.0 0.0 3.9971700036374246E-4 18-19 0.0 0.0 0.0 0.0 3.9971700036374246E-4 20-21 0.0 0.0 0.0 0.0 3.9971700036374246E-4 22-23 0.0 0.0 0.0 0.0 3.9971700036374246E-4 24-25 0.0 0.0 0.0 0.0 3.9971700036374246E-4 26-27 0.0 0.0 0.0 0.0 3.9971700036374246E-4 28-29 0.0 0.0 0.0 0.001798726501636841 7.994340007274849E-4 30-31 0.0 0.0 0.0 0.006995047506365494 0.0011991510010912274 32-33 0.0 0.0 0.0 0.010592500509639176 0.0011991510010912274 34-35 0.0 0.0 0.0 0.02118500101927835 0.0011991510010912274 36-37 0.0 0.0 0.0 0.037973115034555534 0.0011991510010912274 38-39 0.0 0.0 0.0 0.0701503335638368 0.0011991510010912274 40-41 0.0 0.0 0.0 0.11172090160166602 0.0011991510010912274 42-43 0.0 0.0 0.0 0.14249911062967419 0.0011991510010912274 44-45 0.0 0.0 0.0 0.18686769767004963 0.0011991510010912274 46-47 0.0 0.0 0.0 0.24162892671988234 0.0011991510010912274 48-49 0.0 0.0 0.0 0.30978067528190045 0.0011991510010912274 50-51 0.0 0.0 0.0 0.3965192643608325 0.0011991510010912274 52-53 0.0 0.0 0.0 0.464071437422305 0.0013990095012730987 54-55 0.0 0.0 0.0 0.549411016999964 0.0015988680014549699 56-57 0.0 0.0 0.0 0.6543367295954464 0.0015988680014549699 58-59 0.0 0.0 0.0 0.7732525372036598 0.0015988680014549699 60-61 0.0 0.0 0.0 0.9113547608293329 0.0015988680014549699 62-63 0.0 0.0 0.0 1.052454861957734 0.0015988680014549699 64-65 0.0 0.0 0.0 1.1897576515826795 0.0015988680014549699 66-67 0.0 0.0 0.0 1.364633839241817 0.0015988680014549699 68-69 0.0 0.0 0.0 1.5976688504538785 0.0015988680014549699 70-71 0.0 0.0 0.0 1.8560858911890383 0.0015988680014549699 72-73 0.0 0.0 0.0 2.0747310903880054 0.0015988680014549699 74-75 0.0 0.0 0.0 2.267794401563693 0.0015988680014549699 76-77 0.0 0.0 0.0 2.502028563776846 0.0015988680014549699 78-79 0.0 0.0 0.0 2.798218861046379 0.0015988680014549699 80-81 0.0 0.0 0.0 3.0826175068051818 0.0015988680014549699 82-83 0.0 0.0 0.0 3.3814059645770795 0.0015988680014549699 84-85 0.0 0.0 0.0 3.7041774423708014 0.0015988680014549699 86-87 0.0 0.0 0.0 4.017355712155794 0.0015988680014549699 88 0.0 0.0 0.0 4.297757187910959 0.0015988680014549699 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 640 0.0 70.554634 2 ACCTGGG 660 0.0 66.9243 3 GTACCTG 730 0.0 62.56305 1 CCTGGGG 610 0.0 48.53004 4 TGGGGGG 560 0.0 43.63302 6 GGTATCA 415 0.0 43.112637 1 CTGGGGG 435 0.0 42.128433 5 CATGGGG 615 0.0 41.258987 4 ATGGGGG 435 0.0 39.968 5 ATAGGGC 60 8.815989E-5 39.157837 3 TGCACCG 50 0.0017013658 37.591522 5 CTAATAG 50 0.0017013658 37.591522 3 GGATAGG 65 1.3956682E-4 36.2181 1 GTACATG 1110 0.0 36.05496 1 TACATGG 1140 0.0 35.071014 2 ACATGGG 1170 0.0 32.932743 3 GTATACA 105 2.0285133E-6 31.389025 1 GTCTAAA 75 3.234402E-4 31.389023 1 TACAGCG 60 0.0041594035 31.32627 7 GTATCAA 605 0.0 29.543537 2 >>END_MODULE