FastQCFastQC Report
Mon 6 Jun 2016
ERR1211537_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211537_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250177
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7350.2937919952673507No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5200.20785284018914607No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4150.16588255515095313No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3550.14189953512912856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG6100.072.4592062
ACCTGGG6750.064.746193
GTACCTG7000.063.143021
GCGTTAG250.00522184756.425681
TATAACG250.00522184756.425682
CCTGGGG4450.048.5772444
GGTATCA5050.045.624731
CTGGGGG3600.037.8556375
TGGGGGG4600.036.777296
GTTTTAG1055.0644303E-835.825831
ATGGGGG4050.034.809785
CTATAGC550.0027148934.1768723
GTATCAA7050.034.015482
CATGGGG5850.033.738714
TACATGG11450.033.6747132
ATTGGAC702.1819901E-433.5665783
GTACATG11450.033.2640461
CCTGGGA4000.032.895244
ACATGGG11300.031.6060493
CAGTCCG600.0041577631.3288029
Produced by FastQC (version 0.11.5)