##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211537_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 250177 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.057839049952634 33.0 31.0 34.0 30.0 34.0 2 32.25161385738897 33.0 31.0 34.0 30.0 34.0 3 32.261151105017646 34.0 31.0 34.0 30.0 34.0 4 35.930773012707 37.0 35.0 37.0 35.0 37.0 5 35.87520035814643 37.0 35.0 37.0 35.0 37.0 6 35.90554687281405 37.0 35.0 37.0 35.0 37.0 7 35.82048709513664 37.0 35.0 37.0 35.0 37.0 8 35.81122165506821 37.0 35.0 37.0 35.0 37.0 9 37.462632456220994 39.0 37.0 39.0 34.0 39.0 10-11 37.40457955767317 39.0 37.0 39.0 34.0 39.0 12-13 37.30487814627244 39.0 37.0 39.0 34.0 39.0 14-15 38.522032401060045 40.0 38.0 41.0 34.0 41.0 16-17 38.440444165530806 40.0 38.0 41.0 33.5 41.0 18-19 38.42037037777254 40.0 38.0 41.0 33.5 41.0 20-21 38.328353525703804 40.0 38.0 41.0 34.0 41.0 22-23 38.17893331521283 40.0 37.5 41.0 33.5 41.0 24-25 38.075542515898746 40.0 37.0 41.0 33.0 41.0 26-27 37.89539406100481 40.0 37.0 41.0 33.0 41.0 28-29 37.92204918917406 40.0 37.0 41.0 33.0 41.0 30-31 37.826336953436964 40.0 37.0 41.0 33.0 41.0 32-33 37.5753886248536 40.0 36.5 41.0 32.5 41.0 34-35 37.38810122433317 40.0 36.0 41.0 32.0 41.0 36-37 37.297191588355446 40.0 36.0 41.0 31.0 41.0 38-39 37.1861502056544 40.0 36.0 41.0 31.0 41.0 40-41 37.00605970972552 39.5 36.0 41.0 30.5 41.0 42-43 36.86258928678495 39.0 36.0 41.0 30.0 41.0 44-45 36.68018842659397 39.0 35.0 41.0 30.0 41.0 46-47 36.44581436343069 39.0 35.0 41.0 29.5 41.0 48-49 36.225478361320185 39.0 35.0 41.0 28.5 41.0 50-51 36.02556589934326 39.0 35.0 40.0 28.0 41.0 52-53 35.814389412296094 39.0 35.0 40.0 27.5 41.0 54-55 35.62718795093075 38.0 34.0 40.0 27.0 41.0 56-57 35.41271180004557 38.0 34.0 40.0 26.0 41.0 58-59 35.1280253580465 38.0 34.0 40.0 26.0 41.0 60-61 34.80140260695428 37.5 33.5 40.0 26.0 41.0 62-63 34.4379139569185 37.0 33.0 40.0 25.0 41.0 64-65 34.132566143170635 36.5 33.0 39.5 24.5 41.0 66-67 33.799044276652126 36.0 32.0 39.0 23.5 40.5 68-69 33.390147775375034 35.5 32.0 39.0 23.0 40.0 70-71 32.99999000707499 35.0 31.0 38.0 22.5 40.0 72-73 32.60166002470251 35.0 31.0 37.5 22.0 39.5 74-75 32.1851629046635 35.0 31.0 37.0 21.0 39.0 76-77 30.656213400912154 33.5 28.5 35.5 20.0 37.5 78-79 31.49803339235821 34.0 30.0 36.0 20.0 38.0 80-81 31.479492519296336 34.0 30.0 36.0 20.0 37.0 82-83 31.21470398957538 34.0 30.0 35.0 20.0 37.0 84-85 30.914510526547204 34.0 30.0 35.0 19.5 36.5 86-87 30.62816525899663 34.0 30.0 35.0 19.0 36.0 88-89 30.37249427405397 34.0 29.0 35.0 18.5 36.0 90-91 30.08787978111497 34.0 29.0 35.0 18.0 35.0 92-93 29.862149598084557 34.0 29.0 35.0 17.5 35.0 94-95 29.66454750036974 34.0 29.0 35.0 17.0 35.0 96-97 29.465950107324012 33.5 29.0 35.0 15.5 35.0 98-99 29.310328287572396 33.0 27.5 35.0 15.0 35.0 100 27.45385467089301 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2558893251034391 1101 2 0.1937954431705151 1101 3 0.23923518121301157 1101 4 0.0620108403897035 1101 5 -0.08337790216628349 1101 6 0.23786118975617399 1101 7 0.2910208509908472 1101 8 0.4116317495743047 1101 9 0.058792888145816846 1101 10-11 -0.10988683803607557 1101 12-13 -0.12679243308541288 1101 14-15 0.04951476865360149 1101 16-17 0.1472048281951217 1101 18-19 -0.012433636473076604 1101 20-21 -0.02369287848035384 1101 22-23 0.12199168576167807 1101 24-25 0.07112736080161852 1101 26-27 0.11539814327380782 1101 28-29 -0.16808278387136255 1101 30-31 -0.31670737273920935 1101 32-33 -0.2613942965307885 1101 34-35 -0.29831415696320107 1101 36-37 -0.02285331253701628 1101 38-39 0.2724536916012781 1101 40-41 0.03519166145139252 1101 42-43 0.2692710695230076 1101 44-45 0.378650097473745 1101 46-47 0.6448180048781325 1101 48-49 0.3140632487781261 1101 50-51 0.4173170841931295 1101 52-53 0.44004296053731906 1101 54-55 0.25646358682862314 1101 56-57 -0.04942714386147884 1101 58-59 -0.21170671501724314 1101 60-61 -0.2872139808088292 1101 62-63 -0.22856742080582393 1101 64-65 0.04378222042057445 1101 66-67 0.26385810561527023 1101 68-69 0.6074028961418563 1101 70-71 0.33589013177994076 1101 72-73 0.07252327000511372 1101 74-75 0.11631698878238694 1101 76-77 0.29135024935863285 1101 78-79 0.3613968748201728 1101 80-81 6.642051367720114E-4 1101 82-83 -0.012937000098769857 1101 84-85 -0.31565899577006107 1101 86-87 -0.1011379418714391 1101 88-89 0.31582211043674846 1101 90-91 0.18225169345639713 1101 92-93 -0.3169944101022253 1101 94-95 0.21450587509858465 1101 96-97 0.13118748006060343 1101 98-99 0.015264547427086939 1101 100 0.09684139969346006 1102 1 0.20988948029859955 1102 2 0.01774205603619805 1102 3 0.11389957168402276 1102 4 0.04216137969287814 1102 5 0.2567478059116297 1102 6 0.08767883544562238 1102 7 -0.13913356819127642 1102 8 0.12574674918631956 1102 9 0.14331217114418848 1102 10-11 0.14714738249624304 1102 12-13 0.07180305073548254 1102 14-15 0.08385169734143005 1102 16-17 0.3102606208543932 1102 18-19 0.13263503738429705 1102 20-21 0.16652206681335002 1102 22-23 0.25489305923883165 1102 24-25 0.13831910441921735 1102 26-27 0.16407510455261587 1102 28-29 0.12188384916968431 1102 30-31 0.17775991988126094 1102 32-33 0.4820836269579942 1102 34-35 0.49016260256190236 1102 36-37 0.2561565423555052 1102 38-39 0.25548310108370487 1102 40-41 -3.945882576203985E-4 1102 42-43 -0.04706322084712866 1102 44-45 -0.0931897411232896 1102 46-47 0.16861718234379452 1102 48-49 0.40635780918783837 1102 50-51 0.34104642383773864 1102 52-53 -0.07682675312761233 1102 54-55 0.23227642146817828 1102 56-57 0.48657813277389295 1102 58-59 0.6305218874503637 1102 60-61 0.7903991176624956 1102 62-63 0.5193490841992272 1102 64-65 0.45511146694808957 1102 66-67 0.3373352531282663 1102 68-69 0.19234120606658678 1102 70-71 -0.2964990976760973 1102 72-73 -0.40605203844526017 1102 74-75 -0.3359139416125849 1102 76-77 0.18549939190538467 1102 78-79 0.5035013211614512 1102 80-81 0.20520366350567087 1102 82-83 0.1193979110520047 1102 84-85 0.06208601858549301 1102 86-87 0.18375380950591946 1102 88-89 -0.6502832670754728 1102 90-91 0.05916641371712572 1102 92-93 -0.32191409660800474 1102 94-95 -0.42154596008416334 1102 96-97 -0.0035374365219986714 1102 98-99 -0.00926404728075525 1102 100 -0.15474547079618262 1103 1 0.28242746803589114 1103 2 0.10526468031186909 1103 3 0.16563024108197766 1103 4 -0.10890564428535043 1103 5 0.030968057619844558 1103 6 0.0990856795520898 1103 7 0.047192249439525824 1103 8 -0.06456374707453705 1103 9 -0.10768049087369036 1103 10-11 0.06612792211153362 1103 12-13 -0.2420044219400026 1103 14-15 0.04537289050013271 1103 16-17 0.3175565113037919 1103 18-19 -0.11063380214820029 1103 20-21 -0.24843871777548543 1103 22-23 -0.060710324931896764 1103 24-25 -0.18380147420101878 1103 26-27 -0.15031463630297281 1103 28-29 0.03728659635304865 1103 30-31 0.17266682712524073 1103 32-33 -0.20913885560903367 1103 34-35 -0.20819441903113045 1103 36-37 0.06158075629345916 1103 38-39 0.2431291942746654 1103 40-41 0.06115747212990641 1103 42-43 -0.03374120549769799 1103 44-45 -0.07162477262189526 1103 46-47 0.2952359580372601 1103 48-49 0.2033847338297079 1103 50-51 0.06357809782367951 1103 52-53 0.06420140620341641 1103 54-55 0.14076210328317273 1103 56-57 0.31633835052816295 1103 58-59 0.31661620086497777 1103 60-61 0.23732852992239373 1103 62-63 0.062177364193047424 1103 64-65 0.11751518223999113 1103 66-67 0.028810285749315767 1103 68-69 -0.261435118768901 1103 70-71 -0.2789679147597113 1103 72-73 -0.05529508981113196 1103 74-75 -0.1525134954408216 1103 76-77 -0.4099224368376184 1103 78-79 0.03743422703043109 1103 80-81 0.3103486693283557 1103 82-83 0.3279621639949113 1103 84-85 0.19029024739583988 1103 86-87 0.055297030641551004 1103 88-89 0.09642305930337969 1103 90-91 0.05378569451069737 1103 92-93 -0.021512822633287243 1103 94-95 -0.14185379053889235 1103 96-97 0.32281335550303325 1103 98-99 0.13299178592396288 1103 100 -0.2505040329445407 1104 1 -0.06638453493562224 1104 2 -0.10901320504124357 1104 3 -0.027700900150229302 1104 4 -0.055844618625584985 1104 5 -0.013653378693526008 1104 6 -0.1418384128571546 1104 7 -0.04516827812614821 1104 8 0.13607439792077258 1104 9 0.2816123129454411 1104 10-11 0.1264167923269639 1104 12-13 0.26088184454643226 1104 14-15 0.2467600033811621 1104 16-17 0.11021419035583335 1104 18-19 0.14210936884786207 1104 20-21 0.03196146246393994 1104 22-23 0.08610564817832511 1104 24-25 0.22421552676107126 1104 26-27 0.17563253405982238 1104 28-29 0.21881692650783435 1104 30-31 -0.041385758945175155 1104 32-33 -0.07272318452162807 1104 34-35 0.10147031127954165 1104 36-37 0.28206350567540994 1104 38-39 0.25469252772978734 1104 40-41 -0.04196870764674543 1104 42-43 -0.12610173306337202 1104 44-45 0.11545888039285757 1104 46-47 -0.05722954681580461 1104 48-49 -0.004627395796539702 1104 50-51 -0.005387839360444957 1104 52-53 0.35912425642201384 1104 54-55 0.19101602166393405 1104 56-57 0.20547283593241872 1104 58-59 0.4874783055256273 1104 60-61 0.15042998766965354 1104 62-63 0.25542934621781654 1104 64-65 -0.06945277654850912 1104 66-67 -0.2354414161736429 1104 68-69 -0.05804466902282002 1104 70-71 -0.026681431498836616 1104 72-73 0.15591385396147217 1104 74-75 0.018896634502063137 1104 76-77 -0.10590845610375155 1104 78-79 -0.11372941331884334 1104 80-81 -0.20743170649520692 1104 82-83 -0.30929990169505217 1104 84-85 -0.3117861595068412 1104 86-87 -0.37749680224727555 1104 88-89 -0.527223622707993 1104 90-91 -0.5540047893347371 1104 92-93 -0.3894265512755233 1104 94-95 -0.24503477284376274 1104 96-97 -0.44015353159158366 1104 98-99 -0.3912552882299458 1104 100 -0.69848425532102 1105 1 0.34726087372288816 1105 2 0.1994827180804819 1105 3 0.35892305636306787 1105 4 0.12697182478346036 1105 5 0.1489000089981829 1105 6 0.19467391484620578 1105 7 0.14324173934618756 1105 8 0.13118457070275724 1105 9 0.15196515705337532 1105 10-11 0.30886316196392727 1105 12-13 0.35701609032394543 1105 14-15 0.42744093912179437 1105 16-17 0.3349824392395462 1105 18-19 0.5499913335149174 1105 20-21 0.35421078298423225 1105 22-23 0.6281666557496735 1105 24-25 0.5334697977764122 1105 26-27 0.6372843977756091 1105 28-29 0.6771077923591307 1105 30-31 0.7331725792415824 1105 32-33 0.940083794705707 1105 34-35 1.0814802608039074 1105 36-37 0.9314459071508523 1105 38-39 1.0447150961630953 1105 40-41 1.2335785161932833 1105 42-43 0.9483376961736738 1105 44-45 0.9921029786262068 1105 46-47 1.4854017383715359 1105 48-49 1.3725566461370704 1105 50-51 1.4013657036409057 1105 52-53 1.6857962618578526 1105 54-55 1.1579641870687212 1105 56-57 1.4182349209644585 1105 58-59 1.6660844014293374 1105 60-61 1.821677075613735 1105 62-63 1.7514070689900976 1105 64-65 1.6752873224613936 1105 66-67 1.490831394931 1105 68-69 1.725079966970192 1105 70-71 1.5602373706755017 1105 72-73 1.812236655359257 1105 74-75 1.7885408783382033 1105 76-77 1.8501437667655942 1105 78-79 1.915846154532062 1105 80-81 1.6419907357490189 1105 82-83 1.8150266853754147 1105 84-85 1.825960151889003 1105 86-87 1.7844863078284412 1105 88-89 1.8447611860670037 1105 90-91 1.0993210211874924 1105 92-93 1.0931496475208284 1105 94-95 1.4776586362030244 1105 96-97 1.9174044668553236 1105 98-99 1.820681714293837 1105 100 1.9911391187810956 1106 1 0.10292462571017325 1106 2 -0.13905066824702317 1106 3 0.06222194348548271 1106 4 0.0903064829710587 1106 5 0.14224261631137836 1106 6 0.2882748687412757 1106 7 0.16589674729531367 1106 8 0.04722193159305732 1106 9 0.01931412366705132 1106 10-11 0.07828883763960448 1106 12-13 0.13414248142092333 1106 14-15 -0.0979962627859976 1106 16-17 -0.17738838746952013 1106 18-19 -0.03715095583324768 1106 20-21 0.185739002380096 1106 22-23 0.19389598802312236 1106 24-25 0.06432234149659877 1106 26-27 0.15770371415080575 1106 28-29 0.37439515324943073 1106 30-31 0.38023140277099543 1106 32-33 0.06354365162144404 1106 34-35 0.3265617393412654 1106 36-37 0.24936124896325396 1106 38-39 0.27787288630618434 1106 40-41 0.30765443034272977 1106 42-43 0.4430714004662022 1106 44-45 0.5849587019966407 1106 46-47 0.22303091166246958 1106 48-49 0.006996232782540801 1106 50-51 -0.1848823090776932 1106 52-53 -0.23530866660001237 1106 54-55 0.262296460518634 1106 56-57 0.2990974010916361 1106 58-59 -0.22600621860245695 1106 60-61 -0.11885352851980713 1106 62-63 0.08502193385496781 1106 64-65 -0.34920592078184853 1106 66-67 -0.23894801683371014 1106 68-69 0.06222225631835698 1106 70-71 -0.1451978439508892 1106 72-73 -0.2015252683291351 1106 74-75 -0.14856166538198323 1106 76-77 0.1405849432361812 1106 78-79 -0.1392512222564939 1106 80-81 0.25854478980307505 1106 82-83 0.6878903578714457 1106 84-85 0.3256123776441662 1106 86-87 0.10423988493495884 1106 88-89 -0.15290375828912062 1106 90-91 0.2130431992479025 1106 92-93 0.11769257279904721 1106 94-95 0.11552628326184688 1106 96-97 0.20869023792050712 1106 98-99 0.2995171673939936 1106 100 0.45616296615788343 1107 1 0.3254183131692585 1107 2 0.08897233745764055 1107 3 0.13227945774715266 1107 4 0.1501119631917618 1107 5 0.16036194671444548 1107 6 0.20635211553825172 1107 7 0.2903005628756006 1107 8 0.13053578177542136 1107 9 0.20797726778002357 1107 10-11 0.28907509968018985 1107 12-13 0.23482750900906524 1107 14-15 0.10975062770310728 1107 16-17 0.3342255188243186 1107 18-19 0.28874565877443814 1107 20-21 0.33312514284581596 1107 22-23 0.3778422612860055 1107 24-25 0.4416661645584128 1107 26-27 0.42437349812163205 1107 28-29 0.30394602765324663 1107 30-31 0.382826558480339 1107 32-33 0.47457773934238645 1107 34-35 0.5590970428108264 1107 36-37 0.4780505784311302 1107 38-39 0.7122107709035816 1107 40-41 0.7416883777849819 1107 42-43 0.3979484228172723 1107 44-45 0.4560951931590793 1107 46-47 0.36115865878676345 1107 48-49 0.7250652966560978 1107 50-51 0.7722567071011213 1107 52-53 0.3715661580516283 1107 54-55 0.4685178202867135 1107 56-57 0.7086760974350454 1107 58-59 1.1954961661352215 1107 60-61 1.2770187711154648 1107 62-63 1.0793698717582672 1107 64-65 0.9785961459907995 1107 66-67 0.7556454087718265 1107 68-69 0.66409380780064 1107 70-71 0.6959423879765367 1107 72-73 0.26282056539385934 1107 74-75 1.096283092871083 1107 76-77 0.8401956698797797 1107 78-79 0.3980044590303322 1107 80-81 0.5654336423234128 1107 82-83 0.6399618064826882 1107 84-85 0.6634385255914239 1107 86-87 0.5660607022921091 1107 88-89 0.44543160129191506 1107 90-91 0.3563495679002955 1107 92-93 0.7064714468287825 1107 94-95 0.6821352798016385 1107 96-97 0.36647453605947433 1107 98-99 0.5514343094495473 1107 100 0.365706592829536 1108 1 0.08561198339752707 1108 2 0.12817534897899208 1108 3 0.08559834186188198 1108 4 0.04243993510451105 1108 5 0.11212183619060312 1108 6 -0.0767384962720854 1108 7 0.12869746009602778 1108 8 0.24857575794688813 1108 9 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0.7060808739577027 2316 100 0.826433236428155 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 5.0 13 12.0 14 39.0 15 100.0 16 237.0 17 594.0 18 1064.0 19 1533.0 20 1742.0 21 1987.0 22 2214.0 23 2571.0 24 2750.0 25 2921.0 26 3486.0 27 3937.0 28 4862.0 29 5809.0 30 7008.0 31 8478.0 32 10389.0 33 12923.0 34 17396.0 35 22368.0 36 32702.0 37 49338.0 38 46480.0 39 7230.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.970164049758566 18.0754532953855 10.212737040708644 23.741645614147295 2 16.37113451546194 21.136715453138187 38.62784548861804 23.864304542781827 3 17.577625351752367 28.271696725505247 30.932623433103096 23.218054489639293 4 11.163696103158964 15.602953109198687 36.83831847052287 36.39503231711948 5 10.964500281807243 38.71511885870065 35.298255992900856 15.022124866591252 6 30.005795942840013 36.093134805636055 20.085140401718796 13.815928849805136 7 27.90104605938995 32.537763263609364 22.62278306958673 16.938407607413954 8 28.523176054874245 34.30120077386758 20.689765441376334 16.485857729881843 9 28.706875532123256 12.132210395040312 20.040211530236594 39.12070254259984 10-11 25.78514411796448 24.95293332320717 30.359905187127513 18.902017371700836 12-13 24.78345331505294 23.12562705604432 30.173836923458193 21.917082705444546 14-15 22.235857013234632 24.74667935101948 27.34144225888071 25.676021376865176 16-17 22.135216807367613 27.176467766692248 28.346843821949346 22.34147160399079 18-19 22.014745648554715 26.763012365319543 29.433937375962078 21.788304610163664 20-21 22.950102130142984 26.303808195194488 29.25578904029644 21.490300634366093 22-23 22.396942964381218 25.9921975241529 29.31484508967651 22.296014421789373 24-25 22.133929178141877 25.909655963577787 29.406580141259987 22.54983471702035 26-27 21.849817728319262 25.973314786390382 29.441473202865183 22.735394282425172 28-29 22.23803366104317 26.816511139402927 28.959649519028023 21.98580568052588 30-31 23.053431452886226 25.986225421811483 29.46564549847904 21.494697626823253 32-33 22.877323137742405 26.07530373920499 29.317853409733647 21.729519713318957 34-35 22.838760997565647 26.72173610639208 28.808725301696054 21.630777594346224 36-37 22.684302462695815 26.78506473520324 28.75270312943443 21.777929672666517 38-39 22.776719635937027 27.077683155692085 28.326601419397907 21.818995788972988 40-41 22.668348216784057 26.585147462085175 28.791282588361327 21.95522173276944 42-43 22.372457902363465 26.900314795383 28.34177784440114 22.385449457852395 44-45 22.048403956580746 27.067881062736472 28.619054186128785 22.264660794554008 46-47 22.03898650282715 26.68634712897765 28.172775005146633 23.101891363048566 48-49 22.120842660697154 26.690318196354333 28.644267668692038 22.544571474256475 50-51 22.281208462837668 27.090674688440053 28.55518133938298 22.072935509339302 52-53 22.331016866834155 27.12944031051444 28.241903859644914 22.297638963006488 54-55 21.983719168650307 26.935122505960912 28.73109047193625 22.350067853452533 56-57 22.46124081361813 27.164508437380704 27.704348903630184 22.669901845370983 58-59 22.425354415377022 27.10285790806486 28.18234328874285 22.289444387815266 60-61 22.732351406830578 27.192395099828303 28.30510744359985 21.77014604974126 62-63 22.673731803308836 27.250013491985758 28.060532043965885 22.01572266073952 64-65 22.348649891071894 26.983790698139227 28.067675334279375 22.599884076509504 66-67 22.500559650777916 27.11228193607189 27.651145685092505 22.73601272805769 68-69 22.116885634847822 27.09189068180955 28.111077131566798 22.68014655177583 70-71 22.09068760205703 26.79715309603804 28.301551166922827 22.810608134982104 72-73 21.88672381709996 27.708232980290497 27.579714461031706 22.825328741577838 74-75 22.1955962561982 27.63222323707347 27.629625040222084 22.542555466506244 76-77 21.975964007970692 27.11893878676801 27.856447605894473 23.048649599366822 78-79 21.907592553357343 27.055242191778632 28.082813318249233 22.954351936614792 80-81 22.129481390872623 27.27240020866687 27.415508905318408 23.1826094951421 82-83 22.25880217640733 27.451156766092183 27.47434405945542 22.81569699804507 84-85 22.205875003748087 27.66189243485822 27.185735275009744 22.946497286383945 86-87 21.794469421494213 27.76064145594988 27.44059315884271 23.0042959637132 88-89 21.63283745587277 27.34069220282334 27.601357699427098 23.42511264187679 90-91 22.3203755012614 27.084707679144728 27.513103762609 23.081813056984878 92-93 21.96036721898791 27.314511227688566 27.52043215405284 23.20468939927068 94-95 21.696189371826144 27.153624695109762 27.46851133591907 23.681674597145026 96-97 21.328194651559794 27.128201847846512 27.77446837431885 23.769135126274843 98-99 21.52611758309413 27.240962988430383 27.22297291894874 24.009946509526742 100 20.662594247975118 27.869016303000745 27.56798247367453 23.900406975349604 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4.0 1 5.0 2 40.5 3 89.0 4 89.0 5 88.5 6 117.5 7 181.0 8 234.5 9 278.5 10 304.5 11 323.5 12 363.5 13 377.5 14 402.5 15 448.5 16 499.0 17 505.5 18 501.0 19 530.0 20 574.0 21 644.5 22 718.0 23 841.5 24 1052.0 25 1280.5 26 1461.5 27 1713.0 28 2088.5 29 2423.5 30 2797.0 31 3220.5 32 3623.5 33 4138.0 34 4666.0 35 5080.0 36 5637.5 37 6184.5 38 6651.0 39 7057.0 40 7169.5 41 7252.5 42 7502.5 43 7816.5 44 8066.0 45 8351.5 46 8756.5 47 9153.5 48 9470.0 49 9788.5 50 10081.5 51 10168.0 52 9819.0 53 9304.0 54 8839.5 55 8101.0 56 7212.0 57 6460.5 58 5801.0 59 5126.0 60 4184.5 61 3258.5 62 2623.0 63 1995.5 64 1510.5 65 1181.0 66 898.5 67 699.0 68 531.0 69 425.0 70 342.0 71 260.5 72 200.0 73 155.0 74 129.5 75 93.0 76 68.5 77 51.0 78 32.5 79 19.5 80 14.5 81 7.0 82 4.0 83 6.0 84 4.5 85 1.5 86 3.0 87 2.0 88 1.0 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003597453003273682 2 0.07035019206401867 3 3.9971700036374246E-4 4 0.0 5 0.002398302002182455 6 7.994340007274849E-4 7 0.0 8 0.0019985850018187125 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.9971700036374246E-4 18-19 5.995755005456137E-4 20-21 0.002398302002182455 22-23 0.0 24-25 0.0 26-27 3.9971700036374246E-4 28-29 0.002598160502364326 30-31 0.002398302002182455 32-33 0.0013990095012730987 34-35 0.0031977360029099397 36-37 0.0015988680014549699 38-39 1.9985850018187123E-4 40-41 0.004396887004001167 42-43 0.005795896505274266 44-45 0.004596745504183038 46-47 0.005396179504910523 48-49 0.006795189006183622 50-51 0.009793066508911692 52-53 0.004596745504183038 54-55 0.0021984435020005835 56-57 0.0041970285038192955 58-59 0.004596745504183038 60-61 0.008993632508184206 62-63 0.011391934510366662 64-65 0.00479660400436491 66-67 0.008394057007638591 68-69 0.010592500509639176 70-71 0.0041970285038192955 72-73 0.0073947645067292365 74-75 0.0013990095012730987 76-77 0.0041970285038192955 78-79 0.00959320800872982 80-81 0.006995047506365494 82-83 0.0159886800145497 84-85 0.019785991518005253 86-87 0.023383444521278933 88-89 0.01918641601745964 90-91 0.023183586021097064 92-93 0.03237707702946314 94-95 0.0347753790316456 96-97 0.01918641601745964 98-99 0.015588963014185957 100 0.015588963014185957 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 250177.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.52377604932758 #Duplication Level Percentage of deduplicated Percentage of total 1 79.2387411525598 58.259314589314435 2 15.104499081472046 22.2107961560685 3 3.4854180725168207 7.6878329340601725 4 0.9798504954783867 2.881692335655023 5 0.3886441258835398 1.4287291837174028 6 0.22734433021317246 1.00291281724066 7 0.13931342859448836 0.7169994527251606 8 0.08122456169527505 0.4777549187030556 9 0.055128612130889175 0.36479373589995656 >10 0.2877367381654462 3.443520731211938 >50 0.006049700645084533 0.30003568351167603 >100 0.004949755073250981 0.7181447087826637 >500 0.0010999455718335512 0.5074727531093709 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 735 0.2937919952673507 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 520 0.20785284018914607 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 415 0.16588255515095313 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 355 0.14189953512912856 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.9985850018187123E-4 0.0 20-21 0.0 0.0 0.0 3.9971700036374246E-4 0.0 22-23 0.0 0.0 0.0 3.9971700036374246E-4 0.0 24-25 0.0 0.0 0.0 3.9971700036374246E-4 0.0 26-27 0.0 0.0 0.0 3.9971700036374246E-4 0.0 28-29 0.0 0.0 0.0 0.0019985850018187125 0.0 30-31 0.0 0.0 0.0 0.0063954720058198794 0.0 32-33 0.0 0.0 0.0 0.00979306650891169 0.0 34-35 0.0 0.0 0.0 0.020385567018550866 0.0 36-37 0.0 0.0 0.0 0.03717368103382805 0.0 38-39 0.0 0.0 0.0 0.0695507580632912 0.0 40-41 0.0 0.0 0.0 0.11112132610112041 0.0 42-43 0.0 0.0 0.0 0.14189953512912856 0.0 44-45 0.0 0.0 0.0 0.18366996166713967 0.0 46-47 0.0 0.0 0.0 0.23743189821606303 0.0 48-49 0.0 0.0 0.0 0.30358506177626243 0.0 50-51 0.0 0.0 0.0 0.3891244998541033 0.0 52-53 0.0 0.0 0.0 0.4550778049141208 0.0 54-55 0.0 0.0 0.0 0.5388185164903249 0.0 56-57 0.0 0.0 0.0 0.6411460685834429 0.0 58-59 0.0 0.0 0.0 0.7588627251905651 0.0 60-61 0.0 0.0 0.0 0.8955659393149651 0.0 62-63 0.0 0.0 0.0 1.032269153439365 0.0 64-65 0.0 0.0 0.0 1.163776046559036 0.0 66-67 0.0 0.0 0.0 1.3340554887139904 0.0 68-69 0.0 0.0 0.0 1.5596957354193233 0.0 70-71 0.0 0.0 0.0 1.8109178701479354 0.0 72-73 0.0 0.0 0.0 2.0237671728416284 0.0 74-75 0.0 0.0 0.0 2.2106348705116776 0.0 76-77 0.0 0.0 0.0 2.4416712967219207 0.0 78-79 0.0 0.0 0.0 2.7294675369838153 0.0 80-81 0.0 0.0 0.0 3.0074707107367984 0.0 82-83 0.0 0.0 0.0 3.301862281504695 0.0 84-85 0.0 0.0 0.0 3.6136415417884136 0.0 86-87 0.0 0.0 0.0 3.917826179065222 0.0 88 0.0 0.0 0.0 4.189833597812749 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 610 0.0 72.459206 2 ACCTGGG 675 0.0 64.74619 3 GTACCTG 700 0.0 63.14302 1 GCGTTAG 25 0.005221847 56.42568 1 TATAACG 25 0.005221847 56.42568 2 CCTGGGG 445 0.0 48.577244 4 GGTATCA 505 0.0 45.62473 1 CTGGGGG 360 0.0 37.855637 5 TGGGGGG 460 0.0 36.77729 6 GTTTTAG 105 5.0644303E-8 35.82583 1 ATGGGGG 405 0.0 34.80978 5 CTATAGC 55 0.00271489 34.176872 3 GTATCAA 705 0.0 34.01548 2 CATGGGG 585 0.0 33.73871 4 TACATGG 1145 0.0 33.674713 2 ATTGGAC 70 2.1819901E-4 33.566578 3 GTACATG 1145 0.0 33.264046 1 CCTGGGA 400 0.0 32.89524 4 ACATGGG 1130 0.0 31.606049 3 CAGTCCG 60 0.00415776 31.328802 9 >>END_MODULE