FastQCFastQC Report
Mon 6 Jun 2016
ERR1211536_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211536_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5848765
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203390.34774862727430494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179810.30743242376809465No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116590.1993412284473731No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102200.050.5342251
GTATCAA173700.036.7527471
TACCTGG52250.030.7762452
TCAACGC209200.029.6737024
CAACGCA212300.029.3714585
GTACCTG56050.029.0623281
ATCAACG213400.029.0458893
AACGCAG220000.028.5283686
GTACATG213700.028.0008341
TATCAAC222050.027.9511032
TACATGG221900.026.190092
ACATGGG218450.025.5563433
CATGGGG120200.024.9819894
ACGCAGA250900.024.8468237
ACCTGGG65350.024.1617283
CGCAGAG256250.024.0901538
ATGGGGG73950.022.7483275
GCAGAGT282500.021.0881549
GAGTACT169450.020.3517412-13
CAGAGTA273200.019.54375310-11
Produced by FastQC (version 0.11.5)