FastQCFastQC Report
Mon 6 Jun 2016
ERR1211536_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211536_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5848765
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219830.37585712539313854No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201240.34407263755681755No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138840.2373834476167191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111950.048.184521
GTATCAA186100.035.0761031
TACCTGG48200.030.7333162
GTACATG224850.028.0481431
TCAACGC231600.027.6365384
ATCAACG232200.027.5246493
CAACGCA235100.027.2446295
TACATGG232950.026.7282922
GTACCTG58150.026.6890091
AACGCAG243250.026.4859076
TATCAAC245850.026.2631152
ACATGGG230050.025.841243
CATGGGG127150.024.7630164
ACCTGGG61550.023.6689343
ACGCAGA280300.022.8171567
ATGGGGG76250.022.8033965
CGCAGAG287800.022.1572368
TAACGCA6950.020.2853034
GAGTACT187250.019.61337312-13
GCAGAGT319000.019.4892759
Produced by FastQC (version 0.11.5)