FastQCFastQC Report
Mon 6 Jun 2016
ERR1211535_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211535_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5933515
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211100.3557756237238804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193930.3268383074787879No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111260.1875111127215487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108050.049.5460781
GTATCAA185000.036.725541
TCAACGC219350.030.0318784
ATCAACG220550.029.9333953
CAACGCA220800.029.8757065
AACGCAG228650.028.9698876
TATCAAC231300.028.867362
ACGCAGA257100.025.509637
CGCAGAG261300.024.9020378
GTACATG193900.024.355481
TACCTGG44050.023.9109292
TACATGG200450.023.059072
GTACCTG48700.022.7202171
ACATGGG195150.022.5850493
GCAGAGT284250.022.2143849
CATGGGG105650.021.6586594
GAGTACT179150.021.03499612-13
CAGAGTA281450.019.78946110-11
AGAGTAC264650.019.5544810-11
GTACTTT204450.018.37716314-15
Produced by FastQC (version 0.11.5)