##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1211535_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5933515
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.363732290219204	31.0	31.0	34.0	28.0	34.0
2	31.414885780182573	33.0	31.0	34.0	28.0	34.0
3	31.51159877408248	33.0	31.0	34.0	28.0	34.0
4	35.04195523226957	37.0	35.0	37.0	32.0	37.0
5	35.133235864407524	37.0	35.0	37.0	32.0	37.0
6	35.24447448097797	37.0	35.0	37.0	32.0	37.0
7	35.18141641168852	37.0	35.0	37.0	32.0	37.0
8	35.14916723055389	37.0	35.0	37.0	32.0	37.0
9	36.725814462422356	39.0	37.0	39.0	32.0	39.0
10-11	36.717312587901105	39.0	37.0	39.0	32.0	39.0
12-13	36.669955835621884	39.0	37.0	39.0	32.0	39.0
14-15	37.86398517573479	40.0	38.0	41.0	32.0	41.0
16-17	37.847142882422986	40.0	38.0	41.0	32.0	41.0
18-19	37.87084898243284	40.0	38.0	41.0	32.0	41.0
20-21	37.83383601457147	40.0	38.0	41.0	32.0	41.0
22-23	37.693334136679525	40.0	37.5	41.0	32.0	41.0
24-25	37.618661198294774	40.0	37.0	41.0	32.0	41.0
26-27	37.47619404349699	40.0	37.0	41.0	31.0	41.0
28-29	37.322309878714385	40.0	37.0	41.0	31.0	41.0
30-31	37.14793254925621	40.0	36.5	41.0	30.5	41.0
32-33	36.98375692991422	40.0	36.0	41.0	30.0	41.0
34-35	36.818791643739	39.0	36.0	41.0	30.0	41.0
36-37	36.65033643632822	39.0	35.5	41.0	30.0	41.0
38-39	36.52334189767785	39.0	35.0	41.0	30.0	41.0
40-41	36.49117260173776	39.0	35.0	41.0	29.5	41.0
42-43	36.43576236008504	39.0	35.0	41.0	29.0	41.0
44-45	36.25313233386955	39.0	35.0	41.0	28.0	41.0
46-47	36.191000022752114	39.0	35.0	41.0	28.0	41.0
48-49	36.14286995145373	39.0	35.0	41.0	27.5	41.0
50-51	35.17727645417598	38.0	34.0	40.0	25.5	40.5
52-53	35.379218304832804	38.5	34.5	39.5	26.5	40.5
54-55	35.97047795446713	39.0	35.0	40.5	27.0	41.0
56-57	35.943679336784356	39.0	35.0	41.0	27.0	41.0
58-59	35.794962682322364	39.0	35.0	41.0	27.0	41.0
60-61	35.622672058636404	39.0	34.5	40.5	26.0	41.0
62-63	35.386914080439674	38.0	34.0	40.0	26.0	41.0
64-65	35.12034906796393	38.0	34.0	40.0	26.0	41.0
66-67	34.830290392794154	37.0	34.0	40.0	26.0	41.0
68-69	34.55727970688538	37.0	34.0	40.0	26.0	41.0
70-71	34.218223430799455	36.0	33.0	39.0	25.0	41.0
72-73	33.87225683258574	36.0	33.0	39.0	25.0	41.0
74-75	33.505130938406666	35.0	33.0	38.5	24.5	40.0
76-77	33.15649223099629	35.0	32.5	37.0	24.0	39.5
78-79	32.83018859815809	35.0	32.0	37.0	24.0	39.0
80-81	32.47927746032495	35.0	32.0	36.5	23.0	39.0
82-83	32.16711721466955	35.0	32.0	36.0	23.0	37.5
84-85	31.892592249282256	35.0	31.5	36.0	22.0	37.0
86-87	31.591642896327052	35.0	31.0	35.0	21.0	37.0
88-89	31.356303388463665	34.5	31.0	35.0	20.0	36.0
90-91	31.12476415750192	34.0	31.0	35.0	20.0	36.0
92-93	30.970973444914186	34.0	31.0	35.0	20.0	36.0
94-95	30.816113045976962	34.0	30.5	35.0	19.5	35.5
96-97	30.723156425828535	34.0	30.0	35.0	19.0	35.0
98-99	30.548742187388083	34.0	30.0	35.0	19.0	35.0
100	29.18525258636744	33.0	27.0	35.0	15.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.44363807288083734
1101	2	0.4304688653462385
1101	3	0.42900596158766646
1101	4	0.41757961998784054
1101	5	0.4232189314094583
1101	6	0.4102500764100654
1101	7	0.4357774511516226
1101	8	0.5089895067234735
1101	9	0.46609929081672163
1101	10-11	0.4933356646151168
1101	12-13	0.43752521642463904
1101	14-15	0.5084423157562767
1101	16-17	0.511085906848443
1101	18-19	0.48337645423237063
1101	20-21	0.5104728212781069
1101	22-23	0.5397042248997934
1101	24-25	0.47007948550487555
1101	26-27	0.44998099710340256
1101	28-29	0.534973465098858
1101	30-31	0.524685149870372
1101	32-33	0.366905861036102
1101	34-35	0.45468930520907946
1101	36-37	0.45320847561418987
1101	38-39	0.4293558720255817
1101	40-41	0.35289402178842977
1101	42-43	0.2806282623109482
1101	44-45	0.27034212065186125
1101	46-47	0.27136471400553575
1101	48-49	0.4449587812546696
1101	50-51	0.28709106290249764
1101	52-53	0.38238288842571677
1101	54-55	0.4329237730685449
1101	56-57	0.4777665375787308
1101	58-59	0.5240701296442865
1101	60-61	0.37084037720509144
1101	62-63	0.41840246707360507
1101	64-65	0.388636343979222
1101	66-67	0.45455274063841244
1101	68-69	0.4423252057731446
1101	70-71	0.3185199271391639
1101	72-73	0.33493813996295074
1101	74-75	0.22795555193854256
1101	76-77	0.22601818548164232
1101	78-79	0.16140116652110237
1101	80-81	0.16037029164989036
1101	82-83	0.12235102132952136
1101	84-85	0.07335903465302351
1101	86-87	-0.010143800962271854
1101	88-89	0.0922708784241415
1101	90-91	0.0011503906582923662
1101	92-93	-0.05442247120093313
1101	94-95	-0.1003274293561951
1101	96-97	-0.20911116654104944
1101	98-99	-0.16309787455087488
1101	100	-0.14232622390966299
1102	1	0.3602906462879787
1102	2	0.28541961733586163
1102	3	0.2951836699342465
1102	4	0.23242442202501223
1102	5	0.3144589263282924
1102	6	0.3011577864458701
1102	7	0.24057344677946446
1102	8	0.2872316209598438
1102	9	0.3209677494404204
1102	10-11	0.3288194174326975
1102	12-13	0.278098602005322
1102	14-15	0.29492849070354765
1102	16-17	0.3122490264358433
1102	18-19	0.36260442290306827
1102	20-21	0.37567432794003963
1102	22-23	0.34446653390121185
1102	24-25	0.3787222488764215
1102	26-27	0.24185491308308116
1102	28-29	0.32931324213258506
1102	30-31	0.34236275219594603
1102	32-33	0.28915154139999544
1102	34-35	0.2959633236205903
1102	36-37	0.2961363358874607
1102	38-39	0.2904855965290025
1102	40-41	0.29899089938115253
1102	42-43	0.29820558873693415
1102	44-45	0.2223597597044389
1102	46-47	0.11835639414379528
1102	48-49	0.2533856194756936
1102	50-51	0.18854681515120575
1102	52-53	0.30122005793081286
1102	54-55	0.3232056958325842
1102	56-57	0.39267656917433413
1102	58-59	0.27751437895129527
1102	60-61	0.28513052587581456
1102	62-63	0.2811952941986178
1102	64-65	0.3734239534745356
1102	66-67	0.21773894181225728
1102	68-69	0.24792436407296492
1102	70-71	0.27704860309427204
1102	72-73	0.2962810946867833
1102	74-75	0.3304445648922112
1102	76-77	0.2732013526541621
1102	78-79	0.11638581338174703
1102	80-81	0.21377160582031252
1102	82-83	0.2508868890731364
1102	84-85	0.06058942680087753
1102	86-87	0.013668234032127913
1102	88-89	0.057341043992071405
1102	90-91	-0.06593966177892341
1102	92-93	0.010716378335850152
1102	94-95	-0.12577599741120338
1102	96-97	-0.1457479452077841
1102	98-99	-0.1388135898030889
1102	100	-0.050836555965478425
1103	1	0.37661973645339586
1103	2	0.4452711408150485
1103	3	0.38541259414127893
1103	4	0.44366228932412355
1103	5	0.3926526307589029
1103	6	0.37394592712968944
1103	7	0.42914947752787214
1103	8	0.4165186653201687
1103	9	0.40012762172226246
1103	10-11	0.38118556307900064
1103	12-13	0.3600821898817159
1103	14-15	0.5037305054940617
1103	16-17	0.5527447172679132
1103	18-19	0.4471126830306389
1103	20-21	0.46706054880102954
1103	22-23	0.5575144650991888
1103	24-25	0.46445557772536716
1103	26-27	0.47692941715175863
1103	28-29	0.5106000868654803
1103	30-31	0.529070856021022
1103	32-33	0.5083742167620287
1103	34-35	0.6127408522257198
1103	36-37	0.5180574533960538
1103	38-39	0.4848541500004018
1103	40-41	0.36558679233600344
1103	42-43	0.3248400112332064
1103	44-45	0.3854675610505467
1103	46-47	0.43817589043893435
1103	48-49	0.4345691425454987
1103	50-51	0.42610982708074374
1103	52-53	0.43465479621455216
1103	54-55	0.4043882424638525
1103	56-57	0.3767978703256958
1103	58-59	0.401337309416931
1103	60-61	0.4213678235857756
1103	62-63	0.4093148073825219
1103	64-65	0.4497123731666264
1103	66-67	0.407430571672613
1103	68-69	0.4114054542557497
1103	70-71	0.3856658540147464
1103	72-73	0.3716470331111239
1103	74-75	0.38657979945987364
1103	76-77	0.35114642690145814
1103	78-79	0.2748684423593346
1103	80-81	0.23369684679268232
1103	82-83	0.22871366959977024
1103	84-85	0.1809118612719054
1103	86-87	0.2434596631439554
1103	88-89	0.34629604138642733
1103	90-91	0.28434149827345934
1103	92-93	0.13231208866053024
1103	94-95	0.14138930386890536
1103	96-97	0.1690089704181581
1103	98-99	0.116067029736314
1103	100	0.0863507441132505
1104	1	0.42569281243235224
1104	2	0.5027149357954457
1104	3	0.43585625216713453
1104	4	0.4415435109341175
1104	5	0.36895233299998864
1104	6	0.33518223199959607
1104	7	0.3820100451067887
1104	8	0.4320591535988001
1104	9	0.4570608469715296
1104	10-11	0.48916917640815427
1104	12-13	0.4673610398103776
1104	14-15	0.5429142526631168
1104	16-17	0.5755805380825549
1104	18-19	0.45581515102307435
1104	20-21	0.5049385158264386
1104	22-23	0.4958160323062941
1104	24-25	0.5822242077660107
1104	26-27	0.5454887470343479
1104	28-29	0.5280374061319577
1104	30-31	0.5423197647609683
1104	32-33	0.4892072721457339
1104	34-35	0.5028757808379609
1104	36-37	0.5373924282519482
1104	38-39	0.6293368198322256
1104	40-41	0.4386348172962684
1104	42-43	0.28201841644482073
1104	44-45	0.3879458421643349
1104	46-47	0.43958830334539556
1104	48-49	0.4619874391961929
1104	50-51	0.6122695682840273
1104	52-53	0.6741528532294367
1104	54-55	0.7063540266348483
1104	56-57	0.6236959842757912
1104	58-59	0.6647597084368755
1104	60-61	0.7129601082459587
1104	62-63	0.68981848627174
1104	64-65	0.5764528685321153
1104	66-67	0.5613863467826548
1104	68-69	0.4424239142341051
1104	70-71	0.43670337831016326
1104	72-73	0.6459001849915822
1104	74-75	0.6304387029692649
1104	76-77	0.5521235161479225
1104	78-79	0.5126139147607347
1104	80-81	0.47374644445661573
1104	82-83	0.540735846858432
1104	84-85	0.48812281821943415
1104	86-87	0.4138280958315299
1104	88-89	0.5235811495697931
1104	90-91	0.45781558072810213
1104	92-93	0.46992479785340535
1104	94-95	0.48967206285664844
1104	96-97	0.5682323041169077
1104	98-99	0.5038423491804842
1104	100	0.6096547265783592
1105	1	0.31249592690614136
1105	2	0.29944986654131256
1105	3	0.3180074894366598
1105	4	0.2841601220603991
1105	5	0.24332132235799264
1105	6	0.27052270133625456
1105	7	0.3131457532123747
1105	8	0.28187615437692415
1105	9	0.38807843384093843
1105	10-11	0.30860345611914397
1105	12-13	0.3470753370774773
1105	14-15	0.43237731838085836
1105	16-17	0.3500597002183312
1105	18-19	0.33502728116279457
1105	20-21	0.4101487266722401
1105	22-23	0.4921189542529234
1105	24-25	0.3791930603551279
1105	26-27	0.4188920023399447
1105	28-29	0.3715304860645787
1105	30-31	0.46880488355069616
1105	32-33	0.49976903837436026
1105	34-35	0.3988078370946724
1105	36-37	0.38830206868954065
1105	38-39	0.42708058029236895
1105	40-41	0.4042366545929994
1105	42-43	0.38397975141064933
1105	44-45	0.30311509961696714
1105	46-47	0.4192657402744615
1105	48-49	0.45057603111961697
1105	50-51	0.4693278795454603
1105	52-53	0.47750641256660487
1105	54-55	0.45000631630360743
1105	56-57	0.44504882890780095
1105	58-59	0.5817190821673677
1105	60-61	0.500326744364564
1105	62-63	0.538724597603526
1105	64-65	0.4887617622957592
1105	66-67	0.4500463047333412
1105	68-69	0.4450971453084449
1105	70-71	0.4238641758425885
1105	72-73	0.47207453145018263
1105	74-75	0.4646937594013778
1105	76-77	0.43981938028142764
1105	78-79	0.44016861271761343
1105	80-81	0.515585187998532
1105	82-83	0.4426560495846985
1105	84-85	0.3637876177071142
1105	86-87	0.3877059282929949
1105	88-89	0.38439176013727305
1105	90-91	0.28336462091833425
1105	92-93	0.3995305431381446
1105	94-95	0.392572968300513
1105	96-97	0.3513372932167549
1105	98-99	0.4301043109300018
1105	100	0.4936441936924183
1106	1	0.36345143063618934
1106	2	0.3104874150804484
1106	3	0.2766658598050604
1106	4	0.2755031406903896
1106	5	0.2920097263929762
1106	6	0.2559596890330553
1106	7	0.27788035572505265
1106	8	0.31734178282924574
1106	9	0.3392082862138892
1106	10-11	0.3192604239904284
1106	12-13	0.3092478244625241
1106	14-15	0.41371509794367967
1106	16-17	0.4033854759005706
1106	18-19	0.3560589421094562
1106	20-21	0.2878086585878421
1106	22-23	0.43879273839513644
1106	24-25	0.36124619854266626
1106	26-27	0.47684218894503516
1106	28-29	0.49137153084960516
1106	30-31	0.45514606518140965
1106	32-33	0.45561984683988754
1106	34-35	0.5491926141027221
1106	36-37	0.5328698769498601
1106	38-39	0.5767960703828408
1106	40-41	0.4775713433409621
1106	42-43	0.4373845630575772
1106	44-45	0.44239062838028786
1106	46-47	0.5485997820621904
1106	48-49	0.640445386039417
1106	50-51	0.4895238916723912
1106	52-53	0.5168251421143353
1106	54-55	0.5074814556778549
1106	56-57	0.5314356273975918
1106	58-59	0.49994182644864793
1106	60-61	0.4987019830619843
1106	62-63	0.4927328556880255
1106	64-65	0.5596966384215563
1106	66-67	0.5248429387402851
1106	68-69	0.46780355193635614
1106	70-71	0.4966299132432468
1106	72-73	0.4035317404202985
1106	74-75	0.4671078532745838
1106	76-77	0.4708547641350478
1106	78-79	0.41645919274573373
1106	80-81	0.45248585231146166
1106	82-83	0.4555006410108291
1106	84-85	0.3846815281621154
1106	86-87	0.45439210777858463
1106	88-89	0.4120333660776474
1106	90-91	0.5582900747442139
1106	92-93	0.6074295373272385
1106	94-95	0.5745794918977367
1106	96-97	0.47606793023685157
1106	98-99	0.5134143557163959
1106	100	0.43675683539715493
1107	1	0.24402356662980296
1107	2	0.2043366519800287
1107	3	0.20738811550127068
1107	4	0.20579547997602532
1107	5	0.21425804347770594
1107	6	0.138360773396613
1107	7	0.12027829669708012
1107	8	0.17775127978395489
1107	9	0.07513366833544666
1107	10-11	0.20080896266199488
1107	12-13	0.2259508541457862
1107	14-15	0.2332493685616015
1107	16-17	0.25776357825660057
1107	18-19	0.2651389810309581
1107	20-21	0.2930901325460127
1107	22-23	0.35007194089946836
1107	24-25	0.3163402213923945
1107	26-27	0.3123466994116697
1107	28-29	0.36535878936479804
1107	30-31	0.3945954690700333
1107	32-33	0.35375321360201895
1107	34-35	0.47219784844033086
1107	36-37	0.42631115566526034
1107	38-39	0.39861869323415533
1107	40-41	0.37394040171604814
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2316	90-91	-0.03964324527797203
2316	92-93	-0.028064617580785267
2316	94-95	-0.009262628856667732
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2316	98-99	-0.016404926442920242
2316	100	-0.3665839978059431
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	15.0
1	50.0
2	83.0
3	107.0
4	103.0
5	139.0
6	205.0
7	1193.0
8	2280.0
9	2968.0
10	4403.0
11	7092.0
12	10818.0
13	13845.0
14	15793.0
15	17759.0
16	20163.0
17	24272.0
18	29077.0
19	34654.0
20	40984.0
21	49680.0
22	57503.0
23	60430.0
24	60279.0
25	60285.0
26	64758.0
27	71717.0
28	82888.0
29	97533.0
30	116224.0
31	142464.0
32	180020.0
33	234782.0
34	320974.0
35	459417.0
36	709248.0
37	1176624.0
38	1376131.0
39	385967.0
40	588.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.604788864450164	19.337186694912724	13.627067324435792	23.43095711620132
2	15.285565477244958	20.907674299090175	40.867344166994826	22.939416056670044
3	17.81487112413981	26.66725336262767	30.979974448379355	24.53790106485317
4	11.974143914629938	17.671000010621356	39.696165202184226	30.658690872564488
5	12.539621741964133	37.940390962618245	35.937952545957174	13.582034749460442
6	29.081209844612417	38.295403428447	19.027626350479164	13.595760376461419
7	25.552285102046802	33.18980903914848	23.76673995973207	17.49116589907265
8	24.681196541293644	35.70376260639072	21.578301493578255	18.036739358737382
9	25.84975218142204	15.668889367928212	21.349089719477156	37.13226873117259
10-11	23.580919940470675	26.570642198742334	29.94969839260704	19.898739468179958
12-13	24.52048811077157	24.658958474650046	29.48501008378759	21.33554333079079
14-15	22.091627885337623	25.996349205921103	27.31647081589803	24.595552092843242
16-17	21.334047980825872	28.690416897368028	28.02428621632076	21.95124890548534
18-19	21.097220063656795	27.855242650660657	29.917758938230794	21.129778347451754
20-21	21.9791260083234	27.12048703452053	29.931440709315538	20.968946247840535
22-23	21.701405882064808	27.11612199559596	29.60983710241448	21.57263501992475
24-25	21.442356186292	27.196581697945028	29.787404577064514	21.57365753869846
26-27	21.367618608423566	27.325547293190578	29.693316586116193	21.613517512269663
28-29	21.34752871812499	27.352639070136437	29.793981032323476	21.505851179415096
30-31	21.758291011278473	27.177800838740684	29.756719015127388	21.307189134853452
32-33	21.448005760379054	27.341690085976673	29.676959756490305	21.533344397153964
34-35	21.669004443996425	27.49319695840026	29.589501359765475	21.24829723783784
36-37	21.676508588773643	27.40525149938265	29.42976469994268	21.488475211901033
38-39	21.509132190328234	27.582840729927916	29.44159364639799	21.46643343334586
40-41	21.71774591481493	27.329374373404907	29.512424515678976	21.44045519610119
42-43	21.517522365807473	27.434173795561005	29.34161614460324	21.706687694028286
44-45	21.56282294986086	27.625586847237166	29.26923540899936	21.542354793902614
46-47	21.618798493470567	27.48623081490734	28.96284711642563	21.93212357519646
48-49	21.588588267924823	27.56005000420129	29.020177401475532	21.831184326398354
50-51	21.665014393444643	27.760760613716894	28.85951177159339	21.714713221245074
52-53	21.679256383500586	27.849147602278457	28.692771013251654	21.778825000969306
54-55	21.621522751075233	27.82811469416225	28.747825830420044	21.80253672434248
56-57	21.639859149065217	27.927293433884632	28.5148381830843	21.918009233965847
58-59	21.637847957684254	27.843374821294052	28.718712604086917	21.800064616934776
60-61	21.818605572484014	27.94400542834625	28.650229903950304	21.58715909521943
62-63	21.616371189750755	27.847544585437618	28.82724677951085	21.708837445300777
64-65	21.56527562132908	27.72672032202318	28.72808616574715	21.979917890900587
66-67	21.56022302626877	27.78722150373486	28.61423117432002	22.038324295676354
68-69	21.537326196086596	27.842427057251246	28.659751668656654	21.96049507800551
70-71	21.59677702724804	27.671018098029275	28.678610172246405	22.053594702476275
72-73	21.509745179635246	27.874601852400595	28.480699193677083	22.134953774287077
74-75	21.54998909965144	27.912100196206275	28.432164003289156	22.10574670085313
76-77	21.534662895531717	27.789401959987714	28.51191437469298	22.164020769787584
78-79	21.541409066459714	27.732790041805444	28.47629853344663	22.249502358288208
80-81	21.50369802710077	27.947877321992515	28.28802771101276	22.260396939893955
82-83	21.68202478335801	27.90754046276293	28.3510564415424	22.05937831233666
84-85	21.647809109597915	27.916952635184973	28.263031082835898	22.17220717238122
86-87	21.45799941876107	27.89732771693799	28.455076029693082	22.189596834607855
88-89	21.540439594615837	27.72503022597525	28.477432606294506	22.25709757311441
90-91	21.716061620626522	27.74971425103426	28.392860153276082	22.141363975063136
92-93	21.638413054976642	27.753904249737115	28.401309037882445	22.206373657403798
94-95	21.50089303752696	27.706304901411034	28.39492697708293	22.397875083979073
96-97	21.554448983554696	27.664431123028617	28.428054578099854	22.353065315316833
98-99	21.55906720584605	27.54713415774545	28.377884407836124	22.515914228572377
100	21.666001785231945	27.519127846414747	28.347031689384476	22.46783867896883
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1860.0
1	1299.0
2	1359.5
3	2042.5
4	1591.5
5	1307.0
6	1909.5
7	3676.5
8	6270.5
9	7792.5
10	6725.5
11	5072.5
12	5050.5
13	5560.0
14	5954.0
15	6371.5
16	6940.0
17	7582.5
18	8310.5
19	9228.5
20	10512.5
21	12533.0
22	15381.5
23	19321.0
24	24576.0
25	31322.5
26	39814.0
27	48913.0
28	58961.0
29	70653.0
30	83889.5
31	96743.5
32	110255.5
33	125141.0
34	140102.0
35	154182.0
36	169710.5
37	184383.5
38	193474.5
39	201571.0
40	205802.5
41	207481.0
42	210311.0
43	213269.0
44	215662.5
45	219212.0
46	220909.0
47	218518.5
48	218741.0
49	218816.0
50	215440.0
51	205744.0
52	192071.5
53	180729.5
54	168732.0
55	153787.5
56	137537.5
57	121740.5
58	105948.0
59	89295.0
60	73362.5
61	59347.0
62	47143.5
63	36218.5
64	27068.0
65	20243.0
66	15106.5
67	11524.5
68	8799.5
69	6612.0
70	5062.0
71	3820.0
72	2897.5
73	2314.0
74	1873.5
75	1464.5
76	1074.0
77	737.5
78	541.5
79	431.0
80	331.5
81	247.0
82	212.5
83	183.0
84	145.0
85	111.5
86	94.5
87	77.0
88	62.5
89	50.5
90	38.5
91	32.0
92	27.0
93	17.5
94	12.0
95	12.5
96	12.5
97	12.5
98	11.5
99	7.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16610727368178896
2	0.1084180287738381
3	0.03358885921751272
4	0.03485286546001822
5	0.03028558957043169
6	0.002662839817544912
7	0.0043313280576521675
8	0.005291972801956344
9	0.005797575298958543
10-11	0.005140292052855685
12-13	0.008030652994051587
14-15	0.018656732139381127
16-17	0.008763776614704774
18-19	0.018151129642378926
20-21	0.02029994025463827
22-23	0.04761932850932373
24-25	0.016499494818838414
26-27	0.00992666235780983
28-29	0.02753848267005308
30-31	0.015682104115351526
32-33	0.02134485208177615
34-35	0.020931943375891018
36-37	0.022305496826080327
38-39	0.021201598040958856
40-41	0.027875551001387878
42-43	0.03700167607227756
44-45	0.03961395564012225
46-47	0.022541444658014685
48-49	0.01371025437704295
50-51	0.013137238213773791
52-53	0.024016118607604428
54-55	0.02785027087653777
56-57	0.021673493704827577
58-59	0.02753005596176971
60-61	0.02848227399779052
62-63	0.054664056634221025
64-65	0.03398491450683111
66-67	0.021892588120195195
68-69	0.029030010036209565
70-71	0.04370090915755669
72-73	0.04136671096306321
74-75	0.04233578241565075
76-77	0.02421835960640531
78-79	0.01742643273000911
80-81	0.02415094594013835
82-83	0.019330868802050725
84-85	0.020047139006137173
86-87	0.022903793114199593
88-89	0.018833693013331893
90-91	0.029240677743293815
92-93	0.028271606290706266
94-95	0.02078868933507373
96-97	0.014814153162164416
98-99	0.019659510425102153
100	0.04437504582022629
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5933515.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.94932722652837
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.97278414927176	49.3438121802146
2	15.698227452449833	20.391786233712704
3	4.390185044419576	8.554186951050543
4	1.7289409356561896	4.491742023410961
5	0.7514669696231513	2.4403637054990854
6	0.3977505025375065	1.550017652629477
7	0.2454425711336301	1.1158930907515028
8	0.16059889879175	0.8344632343876387
9	0.10246651209846465	0.5989617921639787
>10	0.5081070516435287	5.621471616732682
>50	0.027530991711048308	1.2314391514194412
>100	0.014936480196799218	1.804177281450561
>500	0.0010637897986463284	0.4639987119973099
>1k	3.9892053455041607E-4	0.44294655099042946
>5k	0.0	0.0
>10k+	9.97301336364654E-5	1.114739823588944
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21110	0.3557756237238804	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19393	0.3268383074787879	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11126	0.1875111127215487	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	5.0560249700219855E-5	0.0	0.0	0.0
6	0.0	5.0560249700219855E-5	0.0	0.0	0.0
7	0.0	5.0560249700219855E-5	0.0	0.0	0.0
8	0.0	8.426708283369975E-5	0.0	1.685341656673995E-5	1.685341656673995E-5
9	0.0	8.426708283369975E-5	0.0	1.685341656673995E-5	1.685341656673995E-5
10-11	1.685341656673995E-5	8.426708283369975E-5	0.0	1.685341656673995E-5	1.685341656673995E-5
12-13	1.685341656673995E-5	9.269379111706972E-5	0.0	1.685341656673995E-5	1.685341656673995E-5
14-15	1.685341656673995E-5	1.2640062425054962E-4	0.0	3.37068331334799E-5	5.056024970021985E-5
16-17	5.0560249700219855E-5	1.5168074910065955E-4	0.0	3.37068331334799E-5	8.426708283369975E-5
18-19	5.0560249700219855E-5	1.685341656673995E-4	0.0	3.37068331334799E-5	1.5168074910065955E-4
20-21	5.0560249700219855E-5	1.685341656673995E-4	0.0	7.584037455032978E-5	1.5168074910065955E-4
22-23	5.0560249700219855E-5	1.685341656673995E-4	0.0	1.348273325339196E-4	1.5168074910065955E-4
24-25	5.0560249700219855E-5	2.0224099880087942E-4	0.0	2.5280124850109924E-4	1.5168074910065955E-4
26-27	5.0560249700219855E-5	2.3594783193435932E-4	0.0	4.4661553901860875E-4	1.8538758223413945E-4
28-29	5.0560249700219855E-5	2.443745402177293E-4	0.0	0.0011797391596717966	1.8538758223413945E-4
30-31	5.0560249700219855E-5	2.528012485010993E-4	0.0	0.0035897777287156097	1.8538758223413945E-4
32-33	5.0560249700219855E-5	2.528012485010993E-4	0.0	0.009463193402224483	1.8538758223413945E-4
34-35	5.0560249700219855E-5	2.696546650678392E-4	0.0	0.019044360720416147	2.0224099880087942E-4
36-37	5.0560249700219855E-5	2.9493478991794914E-4	0.0	0.036108444994240346	2.0224099880087942E-4
38-39	5.0560249700219855E-5	3.45495039618169E-4	0.0	0.061211608970399504	2.0224099880087942E-4
40-41	5.0560249700219855E-5	3.707751644682789E-4	0.0	0.09080620846159485	2.0224099880087942E-4
42-43	5.0560249700219855E-5	4.0448199760175884E-4	0.0	0.11989520545578802	2.0224099880087942E-4
44-45	5.0560249700219855E-5	4.0448199760175884E-4	0.0	0.15578455603466074	2.0224099880087942E-4
46-47	5.0560249700219855E-5	4.129087058851288E-4	0.0	0.20184494351156101	2.0224099880087942E-4
48-49	5.0560249700219855E-5	4.297621224518687E-4	0.0	0.25409053486845484	2.0224099880087942E-4
50-51	5.0560249700219855E-5	4.3818883073523874E-4	0.0	0.31219268848229087	2.0224099880087942E-4
52-53	5.0560249700219855E-5	4.3818883073523874E-4	0.0	0.3697723861825579	2.1066770708424938E-4
54-55	5.0560249700219855E-5	4.5504224730197866E-4	0.0	0.43444737225742247	2.1909441536761937E-4
56-57	5.0560249700219855E-5	4.5504224730197866E-4	0.0	0.5145011009494372	2.2752112365098936E-4
58-59	5.0560249700219855E-5	4.5504224730197866E-4	0.0	0.6069505175262893	2.3594783193435932E-4
60-61	5.898695798358983E-5	4.5504224730197866E-4	0.0	0.7066131963937059	2.443745402177293E-4
62-63	6.74136662669598E-5	4.5504224730197866E-4	0.0	0.8107420306513087	2.528012485010993E-4
64-65	6.74136662669598E-5	4.5504224730197866E-4	0.0	0.9189325383014958	2.528012485010993E-4
66-67	6.74136662669598E-5	5.393093301356784E-4	0.0	1.045526976842563	2.528012485010993E-4
68-69	6.74136662669598E-5	5.393093301356784E-4	0.0	1.1954296904954314	2.528012485010993E-4
70-71	7.584037455032978E-5	5.730161632691584E-4	0.0	1.361638084676621	2.528012485010993E-4
72-73	8.426708283369975E-5	6.067229964026382E-4	0.0	1.5298183285961189	4.0448199760175884E-4
74-75	8.426708283369975E-5	6.067229964026382E-4	0.0	1.702666968904604	4.0448199760175884E-4
76-77	8.426708283369975E-5	6.067229964026382E-4	0.0	1.8968857414197149	4.0448199760175884E-4
78-79	8.426708283369975E-5	6.067229964026382E-4	0.0	2.121213142631307	4.0448199760175884E-4
80-81	8.426708283369975E-5	6.320031212527482E-4	0.0	2.3601608827145464	4.0448199760175884E-4
82-83	8.426708283369975E-5	6.572832461028581E-4	0.0	2.618102423268501	4.0448199760175884E-4
84-85	8.426708283369975E-5	6.572832461028581E-4	0.0	2.8803837185883916	4.0448199760175884E-4
86-87	8.426708283369975E-5	6.572832461028581E-4	0.0	3.152709650182059	4.2133541416849876E-4
88	8.426708283369975E-5	6.572832461028581E-4	0.0	3.372503482337198	4.2133541416849876E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10805	0.0	49.546078	1
GTATCAA	18500	0.0	36.72554	1
TCAACGC	21935	0.0	30.031878	4
ATCAACG	22055	0.0	29.933395	3
CAACGCA	22080	0.0	29.875706	5
AACGCAG	22865	0.0	28.969887	6
TATCAAC	23130	0.0	28.86736	2
ACGCAGA	25710	0.0	25.50963	7
CGCAGAG	26130	0.0	24.902037	8
GTACATG	19390	0.0	24.35548	1
TACCTGG	4405	0.0	23.910929	2
TACATGG	20045	0.0	23.05907	2
GTACCTG	4870	0.0	22.720217	1
ACATGGG	19515	0.0	22.585049	3
GCAGAGT	28425	0.0	22.214384	9
CATGGGG	10565	0.0	21.658659	4
GAGTACT	17915	0.0	21.034996	12-13
CAGAGTA	28145	0.0	19.789461	10-11
AGAGTAC	26465	0.0	19.55448	10-11
GTACTTT	20445	0.0	18.377163	14-15
>>END_MODULE