FastQCFastQC Report
Mon 6 Jun 2016
ERR1211535_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211535_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5933515
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232160.3912689190134347No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221270.3729155483722549No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135670.22865030256096092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112150.051.9513931
GTATCAA191900.037.0266721
TCAACGC238300.029.2845364
ATCAACG239900.029.0502933
CAACGCA240950.029.0402285
TATCAAC248700.028.2480852
AACGCAG249150.028.1405726
TACCTGG47500.025.0461582
ACGCAGA279750.024.978267
CGCAGAG285500.024.3599788
GTACATG199150.023.730721
GTACCTG51550.023.5351091
TACATGG203000.022.654632
ACATGGG200150.022.140923
GCAGAGT314500.021.7996049
GAGTACT199450.020.8163612-13
AGAGTAC293750.020.15644610-11
CATGGGG111000.020.1520824
CAGAGTA307450.019.92314310-11
ACCTGGG57800.019.675563
Produced by FastQC (version 0.11.5)