FastQCFastQC Report
Mon 6 Jun 2016
ERR1211534_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211534_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6929406
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251750.3633067538545151No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231760.3344586823170702No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145700.21026333281669454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123900.051.702671
GTATCAA212100.036.4207231
TACCTGG61950.029.3774972
TCAACGC258100.029.0566164
ATCAACG260650.028.7914163
CAACGCA260800.028.755185
GTACCTG67550.028.2406711
GTACATG256550.028.1275651
AACGCAG270900.027.7630086
TATCAAC275450.027.4529112
TACATGG264300.026.8139762
ACATGGG261700.026.3954733
CATGGGG152750.024.794424
ACGCAGA305950.024.4912017
CGCAGAG311950.023.8404568
ACCTGGG74900.023.7151473
GCAGAGT346800.020.769639
ATGGGGG90250.020.617635
GAGTACT205650.020.60903412-13
CAGAGTA334650.019.66280410-11
Produced by FastQC (version 0.11.5)