FastQCFastQC Report
Mon 6 Jun 2016
ERR1211534_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211534_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6929406
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280370.4046089953453442No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262650.37903681787443255No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178580.2577132874015464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA138250.052.0731241
GTATCAA231450.036.1428451
TACCTGG58350.029.7362352
TCAACGC287950.028.4930654
ATCAACG288150.028.4569843
CAACGCA292450.028.102845
GTACCTG64500.027.630141
TATCAAC301100.027.4229532
AACGCAG303450.027.1301826
GTACATG262550.026.0946271
TACATGG270900.024.9429742
ACATGGG267600.024.1450793
ACGCAGA341700.023.9966137
CGCAGAG345050.023.6138388
ACCTGGG74000.022.923853
CATGGGG150800.022.7163334
TATAACG6150.022.1729432
ATGGGGG89700.021.1117695
GAGTACT232350.021.06371112-13
GCAGAGT381700.021.01389
Produced by FastQC (version 0.11.5)