##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211534_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6929406 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.134654398948484 33.0 31.0 34.0 30.0 34.0 2 32.355694124431444 34.0 31.0 34.0 31.0 34.0 3 32.36853548485974 34.0 31.0 34.0 30.0 34.0 4 36.00171645304085 37.0 35.0 37.0 35.0 37.0 5 35.92543689892034 37.0 35.0 37.0 35.0 37.0 6 35.94773953207533 37.0 35.0 37.0 35.0 37.0 7 35.87468651714158 37.0 35.0 37.0 35.0 37.0 8 35.87238314510652 37.0 35.0 37.0 35.0 37.0 9 37.52757711122714 39.0 37.0 39.0 35.0 39.0 10-11 37.4951420078431 39.0 37.0 39.0 35.0 39.0 12-13 37.409863269665536 39.0 37.0 39.0 34.0 39.0 14-15 38.65027406389523 40.0 38.0 41.0 34.0 41.0 16-17 38.578536313213576 40.0 38.0 41.0 34.0 41.0 18-19 38.5757288575673 40.0 38.0 41.0 34.0 41.0 20-21 38.52123226723907 40.0 38.0 41.0 34.0 41.0 22-23 38.41447167332957 40.0 38.0 41.0 34.0 41.0 24-25 38.30313817663448 40.0 38.0 41.0 34.0 41.0 26-27 38.12574706980656 40.0 38.0 41.0 33.0 41.0 28-29 38.1621257291029 40.0 38.0 41.0 33.5 41.0 30-31 38.07886166577626 40.0 37.5 41.0 33.0 41.0 32-33 37.89174714254007 40.0 37.0 41.0 33.0 41.0 34-35 37.780067295811506 40.0 37.0 41.0 33.0 41.0 36-37 37.71911272048427 40.0 37.0 41.0 33.0 41.0 38-39 37.62293795456638 40.0 37.0 41.0 32.5 41.0 40-41 37.460377628327734 40.0 36.5 41.0 32.0 41.0 42-43 37.305807958142445 40.0 36.0 41.0 31.0 41.0 44-45 37.11708377312572 40.0 36.0 41.0 31.0 41.0 46-47 36.850931667158775 39.0 36.0 41.0 30.0 41.0 48-49 36.62070392469427 39.0 35.0 41.0 30.0 41.0 50-51 36.41980200034462 39.0 35.0 41.0 29.5 41.0 52-53 36.2235973761676 39.0 35.0 40.0 28.5 41.0 54-55 36.02862979886011 39.0 35.0 40.0 28.0 41.0 56-57 35.833078621746225 38.0 35.0 40.0 28.0 41.0 58-59 35.57455545251642 38.0 34.0 40.0 27.5 41.0 60-61 35.265225691783684 38.0 34.0 40.0 26.5 41.0 62-63 34.947549688963235 37.5 34.0 40.0 26.0 41.0 64-65 34.63483074884052 37.0 33.0 40.0 26.0 41.0 66-67 34.29701377001145 36.5 33.0 39.0 26.0 41.0 68-69 33.8979141213547 36.0 32.5 39.0 25.0 40.0 70-71 33.51174732726008 35.5 32.0 38.5 24.5 40.0 72-73 33.10493958645229 35.0 32.0 38.0 24.0 40.0 74-75 32.69364646839859 35.0 31.0 37.0 23.0 39.0 76-77 31.147687911489093 33.5 29.5 35.5 21.0 38.5 78-79 31.971024644825256 34.0 31.0 36.0 22.5 39.0 80-81 31.934818583295595 34.0 31.0 36.0 22.5 37.5 82-83 31.649794008317595 34.0 31.0 35.5 21.5 37.0 84-85 31.345957936365686 34.0 30.5 35.0 20.5 37.0 86-87 31.04551017792867 34.0 30.0 35.0 20.0 36.0 88-89 30.766613184449 34.0 30.0 35.0 20.0 36.0 90-91 30.48264786332335 34.0 29.5 35.0 19.0 35.5 92-93 30.269201790167873 34.0 29.0 35.0 19.0 35.0 94-95 30.047603863880973 34.0 29.0 35.0 18.0 35.0 96-97 29.839662808038668 34.0 29.0 35.0 18.0 35.0 98-99 29.671850732948826 34.0 29.0 35.0 17.5 35.0 100 27.77071584490792 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32930068369080345 1101 2 0.22460973879883994 1101 3 0.2630221938143791 1101 4 0.16356380004380355 1101 5 0.16921765277351142 1101 6 0.1653702939402777 1101 7 0.1730233568835189 1101 8 0.1507883270187662 1101 9 0.16243888964521602 1101 10-11 0.16524319685807853 1101 12-13 0.21350890744442808 1101 14-15 0.26950815823045104 1101 16-17 0.28303979998464257 1101 18-19 0.27329534286177903 1101 20-21 0.22283489606402185 1101 22-23 0.2235825675360701 1101 24-25 0.25462636462406607 1101 26-27 0.21177808002047271 1101 28-29 0.294736047904955 1101 30-31 0.3240449521979727 1101 32-33 0.43209187538877103 1101 34-35 0.3753781884276819 1101 36-37 0.38159007662723354 1101 38-39 0.37594447360817895 1101 40-41 0.421169940757963 1101 42-43 0.4001611210256968 1101 44-45 0.3259369981585394 1101 46-47 0.4170935935625053 1101 48-49 0.4338022677296891 1101 50-51 0.3910878083945377 1101 52-53 0.42498008130345966 1101 54-55 0.3438494203298177 1101 56-57 0.37284514196819174 1101 58-59 0.4478800775455909 1101 60-61 0.40234833712931106 1101 62-63 0.46672663304052975 1101 64-65 0.4282560647011522 1101 66-67 0.3706413514167224 1101 68-69 0.4397605269227043 1101 70-71 0.3111947309689995 1101 72-73 0.32217623995916256 1101 74-75 0.3226161939964456 1101 76-77 0.34438441270356535 1101 78-79 0.3695326907035188 1101 80-81 0.3527387903491892 1101 82-83 0.33673812181532625 1101 84-85 0.3838593496245686 1101 86-87 0.30561291452882955 1101 88-89 0.32015189202739336 1101 90-91 0.35549111973016423 1101 92-93 0.232980552015011 1101 94-95 0.28010632234819965 1101 96-97 0.2471118562682122 1101 98-99 0.11428818244115035 1101 100 0.23763315318850786 1102 1 0.31443025478562703 1102 2 0.24680123391053144 1102 3 0.32249949612489104 1102 4 0.21269438672746332 1102 5 0.20682320517488506 1102 6 0.17800968025455433 1102 7 0.20462082923483393 1102 8 0.2072609563195229 1102 9 0.22433632115143354 1102 10-11 0.1852816295808637 1102 12-13 0.28658052386412436 1102 14-15 0.3111992314402343 1102 16-17 0.31336289824795927 1102 18-19 0.26300919156541624 1102 20-21 0.2969495306533645 1102 22-23 0.2467776687265939 1102 24-25 0.25658073134833614 1102 26-27 0.2564472936301385 1102 28-29 0.3031999768306122 1102 30-31 0.37880012689208087 1102 32-33 0.40591998768238113 1102 34-35 0.3962974194004758 1102 36-37 0.41761734314774657 1102 38-39 0.422278138144911 1102 40-41 0.4147248375014527 1102 42-43 0.3692603362828777 1102 44-45 0.398749451289099 1102 46-47 0.3930539324977005 1102 48-49 0.3808560842671582 1102 50-51 0.36393929558337135 1102 52-53 0.43090190839278364 1102 54-55 0.348994170750089 1102 56-57 0.3743955501466374 1102 58-59 0.4713292078157849 1102 60-61 0.42587841032168683 1102 62-63 0.47884385091089143 1102 64-65 0.4501460656699692 1102 66-67 0.41906783929567837 1102 68-69 0.4020841857291728 1102 70-71 0.39282418663202634 1102 72-73 0.4070625427146979 1102 74-75 0.3736618759768291 1102 76-77 0.48522558083468326 1102 78-79 0.5450308101234747 1102 80-81 0.5069443672611378 1102 82-83 0.43847657041547095 1102 84-85 0.3482977752438501 1102 86-87 0.34898748974772076 1102 88-89 0.29097387192977564 1102 90-91 0.3560669600042061 1102 92-93 0.2995812007188583 1102 94-95 0.3439783936729164 1102 96-97 0.4162434944098301 1102 98-99 0.3923804754828346 1102 100 0.41528513501973663 1103 1 0.2846792588044096 1103 2 0.249965718074165 1103 3 0.2936251030373356 1103 4 0.12970170278898507 1103 5 0.18554214502563582 1103 6 0.18866467514829566 1103 7 0.1831421413159191 1103 8 0.20293005005503773 1103 9 0.19708861025262792 1103 10-11 0.21630622674435784 1103 12-13 0.23453305485892173 1103 14-15 0.3154723655657037 1103 16-17 0.33883893021695854 1103 18-19 0.3114415885776509 1103 20-21 0.2601172214757028 1103 22-23 0.26826576749167685 1103 24-25 0.291002643980832 1103 26-27 0.3218375126240076 1103 28-29 0.3789079687303101 1103 30-31 0.4243696225055871 1103 32-33 0.4113398508417063 1103 34-35 0.4311621646279562 1103 36-37 0.4571459468918704 1103 38-39 0.4268285436826673 1103 40-41 0.4412079171485743 1103 42-43 0.5095265586646605 1103 44-45 0.4913393684388012 1103 46-47 0.5964328619616595 1103 48-49 0.557125938538455 1103 50-51 0.5342161835908144 1103 52-53 0.46822636997038813 1103 54-55 0.5544911505655676 1103 56-57 0.5883692625163235 1103 58-59 0.6090721906357572 1103 60-61 0.6026092217806109 1103 62-63 0.5977483238512278 1103 64-65 0.5804499488639436 1103 66-67 0.440817786141821 1103 68-69 0.5640243710879815 1103 70-71 0.5881971001254342 1103 72-73 0.5120583949713904 1103 74-75 0.4899398514598303 1103 76-77 0.519751797012276 1103 78-79 0.5269341886172434 1103 80-81 0.56758054046254 1103 82-83 0.6128958863515201 1103 84-85 0.5844507607476608 1103 86-87 0.5403503371212253 1103 88-89 0.5830614415961826 1103 90-91 0.6224553386548308 1103 92-93 0.5417795221027433 1103 94-95 0.6265912454032936 1103 96-97 0.6399887149198982 1103 98-99 0.5814886409603695 1103 100 0.720455697892902 1104 1 0.24129580814401663 1104 2 0.22069915557815278 1104 3 0.28451361365332417 1104 4 0.08619003325021168 1104 5 0.1638726717748611 1104 6 0.1534371245737418 1104 7 0.17396490440860646 1104 8 0.14769894052722066 1104 9 0.17675365826228528 1104 10-11 0.23045849752552527 1104 12-13 0.23569037852606556 1104 14-15 0.3165138163357639 1104 16-17 0.2538252444552853 1104 18-19 0.29256118630557637 1104 20-21 0.2123422742306289 1104 22-23 0.2583286147439594 1104 24-25 0.2965476104242484 1104 26-27 0.3700113592145087 1104 28-29 0.40416315637026656 1104 30-31 0.30454524408953176 1104 32-33 0.32120802300537576 1104 34-35 0.4652460048764766 1104 36-37 0.38544863483269864 1104 38-39 0.45238999270083724 1104 40-41 0.5273320946148203 1104 42-43 0.5565646784337801 1104 44-45 0.44412074042217853 1104 46-47 0.38784670615192596 1104 48-49 0.5207092423575403 1104 50-51 0.46213696515349767 1104 52-53 0.5058852167108938 1104 54-55 0.5060263974800208 1104 56-57 0.5266838774337899 1104 58-59 0.5424563361886712 1104 60-61 0.49610038940351586 1104 62-63 0.5388944102613706 1104 64-65 0.5844484863134767 1104 66-67 0.5554164520861278 1104 68-69 0.565625356904242 1104 70-71 0.46020180297870183 1104 72-73 0.5226598942839047 1104 74-75 0.5346258203900334 1104 76-77 0.46386113447104904 1104 78-79 0.5092973939374694 1104 80-81 0.6271952837713108 1104 82-83 0.4408549732179132 1104 84-85 0.5743624731869943 1104 86-87 0.593379870225867 1104 88-89 0.6694397721229315 1104 90-91 0.61668173877705 1104 92-93 0.5210349370053073 1104 94-95 0.5654871991286257 1104 96-97 0.5242893771541972 1104 98-99 0.5899315333823836 1104 100 0.753981020431663 1105 1 0.25394121837168626 1105 2 0.17866100797954942 1105 3 0.2007923194405663 1105 4 0.08769668501474115 1105 5 0.0722798139555394 1105 6 0.1133193391247076 1105 7 0.12767900764345086 1105 8 0.1613164953874744 1105 9 0.1347254509727449 1105 10-11 0.16547453634614584 1105 12-13 0.16347908253126775 1105 14-15 0.25971127491013846 1105 16-17 0.29328922147019654 1105 18-19 0.3017347632610452 1105 20-21 0.20958427830319692 1105 22-23 0.28289894752386857 1105 24-25 0.2857508513731659 1105 26-27 0.28271922449196296 1105 28-29 0.37088489293557103 1105 30-31 0.4193336371653871 1105 32-33 0.39994823714496874 1105 34-35 0.421647501454224 1105 36-37 0.45279864467360653 1105 38-39 0.41368502425815024 1105 40-41 0.46197095588538417 1105 42-43 0.3663833251481421 1105 44-45 0.4193948725940544 1105 46-47 0.49241680458684556 1105 48-49 0.5659052853967168 1105 50-51 0.6089920299406586 1105 52-53 0.5911908026596535 1105 54-55 0.4855594946538986 1105 56-57 0.49316221717486286 1105 58-59 0.5760883250814217 1105 60-61 0.5096062785264266 1105 62-63 0.5972013338942261 1105 64-65 0.5663436120415852 1105 66-67 0.5569880413386983 1105 68-69 0.5569107935122659 1105 70-71 0.5733240858985837 1105 72-73 0.4937301026164249 1105 74-75 0.49920266697530025 1105 76-77 0.6305623781655747 1105 78-79 0.7150982099541601 1105 80-81 0.6374770204555453 1105 82-83 0.67777019693143 1105 84-85 0.6854415651649184 1105 86-87 0.7192892259726236 1105 88-89 0.7185838772056137 1105 90-91 0.7305713167922789 1105 92-93 0.7342262144667977 1105 94-95 0.8179323084341128 1105 96-97 0.8116410340955156 1105 98-99 0.7616626790220025 1105 100 0.9148786982505541 1106 1 0.1857011213894424 1106 2 0.15090577577049658 1106 3 0.17630307739444362 1106 4 0.12137945672305506 1106 5 0.13356494345998016 1106 6 0.14705692881347687 1106 7 0.13606457482767098 1106 8 0.1369846680557032 1106 9 0.09810447070793771 1106 10-11 0.11919651091761096 1106 12-13 0.18716144438058535 1106 14-15 0.2658447821921541 1106 16-17 0.23135946595187562 1106 18-19 0.26533352126686793 1106 20-21 0.2563962115035352 1106 22-23 0.22497093977522553 1106 24-25 0.324746893233403 1106 26-27 0.29424924078548287 1106 28-29 0.3095924426350152 1106 30-31 0.29640929623808887 1106 32-33 0.37747865492080024 1106 34-35 0.3452934546615509 1106 36-37 0.3911631464430201 1106 38-39 0.38906953367531827 1106 40-41 0.482159766960109 1106 42-43 0.49939786127513486 1106 44-45 0.5332989896285198 1106 46-47 0.5083156731384477 1106 48-49 0.5289788994683917 1106 50-51 0.5808788442383346 1106 52-53 0.5457552759166759 1106 54-55 0.44870918517163716 1106 56-57 0.5183455885989261 1106 58-59 0.5941263443337448 1106 60-61 0.6152684550050864 1106 62-63 0.5858889089070871 1106 64-65 0.6515066531221123 1106 66-67 0.6138815818817633 1106 68-69 0.6737343595632055 1106 70-71 0.6062927192261469 1106 72-73 0.6567633695985933 1106 74-75 0.570244138739703 1106 76-77 0.519713602425302 1106 78-79 0.6365640969536877 1106 80-81 0.6451866634627343 1106 82-83 0.7121564601896146 1106 84-85 0.695469049650864 1106 86-87 0.6249895849784828 1106 88-89 0.6731464234742326 1106 90-91 0.7203131216405865 1106 92-93 0.6547217264228244 1106 94-95 0.7606371447931117 1106 96-97 0.7172189545463041 1106 98-99 0.7961685739434863 1106 100 0.748495181557967 1107 1 0.17068135428953468 1107 2 0.09328760148370208 1107 3 0.19274477923093514 1107 4 0.14333062587857626 1107 5 0.10349013128005424 1107 6 0.10103872066262909 1107 7 0.1364199925634466 1107 8 0.13761668203830624 1107 9 0.14571270279470383 1107 10-11 0.11956146918389976 1107 12-13 0.1863611917156831 1107 14-15 0.2171302442561398 1107 16-17 0.22046900752700793 1107 18-19 0.1904373670837387 1107 20-21 0.18218884845095573 1107 22-23 0.22283831905005513 1107 24-25 0.24204130042846117 1107 26-27 0.23213374834621447 1107 28-29 0.29687962857174455 1107 30-31 0.19274037927242915 1107 32-33 0.2310043496182672 1107 34-35 0.18160319610069564 1107 36-37 0.24310500755800746 1107 38-39 0.2858654120113826 1107 40-41 0.2916568102398003 1107 42-43 0.3316945126491717 1107 44-45 0.307922254742401 1107 46-47 0.31805701555106936 1107 48-49 0.3609197462095537 1107 50-51 0.32783439500339995 1107 52-53 0.3395642577185427 1107 54-55 0.35365214018538893 1107 56-57 0.3886244900489544 1107 58-59 0.510346074516633 1107 60-61 0.528188621287832 1107 62-63 0.4896288513853406 1107 64-65 0.588070909535972 1107 66-67 0.4849867899984588 1107 68-69 0.43325316228695243 1107 70-71 0.512194255745726 1107 72-73 0.4880805118514644 1107 74-75 0.479706544178093 1107 76-77 0.3996137200173955 1107 78-79 0.5077486629393881 1107 80-81 0.5990937050354965 1107 82-83 0.58896911262487 1107 84-85 0.509605419901245 1107 86-87 0.4588189115879686 1107 88-89 0.4527275197949727 1107 90-91 0.4038278991573492 1107 92-93 0.4804608678130684 1107 94-95 0.5917059079073859 1107 96-97 0.5593902132769415 1107 98-99 0.5835450486940701 1107 100 0.7839395625864611 1108 1 0.22716901171258996 1108 2 0.16290685468545973 1108 3 0.2067263072434784 1108 4 0.16317602006439103 1108 5 0.11901860575480327 1108 6 0.15944863990161906 1108 7 0.14225022946580168 1108 8 0.14972226779675424 1108 9 0.09583351436418752 1108 10-11 0.15431097553157969 1108 12-13 0.17625258186090775 1108 14-15 0.21645607255143062 1108 16-17 0.270958757579038 1108 18-19 0.2664852044043613 1108 20-21 0.311785589076635 1108 22-23 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-0.49093096322587115 2316 60-61 -0.31363609611970844 2316 62-63 -0.260406240027784 2316 64-65 -0.33064822218221224 2316 66-67 -0.08913586728075984 2316 68-69 -0.10726479353001395 2316 70-71 -0.2519447414509983 2316 72-73 -0.24924818110708458 2316 74-75 -0.4128798973962873 2316 76-77 -0.3725359034276927 2316 78-79 -0.2764883945330183 2316 80-81 -0.21581535190518863 2316 82-83 -0.10251494477768475 2316 84-85 -0.115042477122838 2316 86-87 -0.11373337762387337 2316 88-89 -0.07569207009025902 2316 90-91 -0.015765238317801078 2316 92-93 0.15734995828627163 2316 94-95 0.1890222401616306 2316 96-97 0.08324335538503291 2316 98-99 -0.06631192920063356 2316 100 -0.2732163691140599 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 2.0 10 5.0 11 28.0 12 101.0 13 190.0 14 606.0 15 1707.0 16 4010.0 17 7977.0 18 14619.0 19 22800.0 20 32305.0 21 44418.0 22 58858.0 23 67624.0 24 71476.0 25 77076.0 26 87955.0 27 103828.0 28 125951.0 29 151822.0 30 183168.0 31 222592.0 32 271844.0 33 343081.0 34 453039.0 35 620320.0 36 907646.0 37 1406027.0 38 1418567.0 39 229732.0 40 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.09224877532336 20.367847490091172 14.115242562752051 23.42466117183342 2 15.01930415506865 21.504243881481802 40.09493586341597 23.381516100033576 3 17.120768510089537 27.310229117972867 31.16530598225867 24.403696389678924 4 11.902061866084486 17.82080984749805 38.58925377459428 31.68787451182318 5 12.275393752291901 38.61424246311997 35.63673812032483 13.473625664263295 6 30.020036476662643 38.412309820153716 18.183117002690867 13.384536700492772 7 26.26210673757606 33.38610841968273 23.107233145236403 17.24455169750481 8 25.529993367326025 36.39034758328797 20.56825968217427 17.51139936721173 9 26.483674418611365 15.198427685163551 20.693780025142143 37.62411787108294 10-11 24.272484539078818 26.702180533223196 29.328473465113746 19.696861462584238 12-13 24.91274865406934 24.6136393220429 29.19279805512911 21.28081396875865 14-15 22.326747776072004 25.90429829050282 26.861638645505835 24.90731528791934 16-17 21.66602735669427 28.83885434498436 27.536696825356966 21.958421472964403 18-19 21.55791807318773 27.784305584478503 29.625262525343356 21.032513816990413 20-21 22.334833629483978 27.042643888623306 29.646895830138813 20.975626651753903 22-23 22.150529363594035 26.911608795299973 29.401945087497044 21.535916753608948 24-25 21.87029360678061 27.054390247703843 29.52403987413636 21.551276271379184 26-27 21.809438039644014 27.104215238431006 29.5491330447415 21.537213677183484 28-29 21.797288312781347 27.17336386630817 29.616872547294914 21.412475273615563 30-31 22.108833204664574 27.096969437640194 29.52079588512043 21.273401472574793 32-33 21.90444062806673 27.174637764821334 29.436507821970793 21.484413785141143 34-35 22.080210936333046 27.326371876310144 29.385108177751672 21.208309009605138 36-37 22.1307523909149 27.205180145450402 29.290952546673704 21.37311491696099 38-39 22.011314862858303 27.378269327043895 29.2502640601285 21.360151749969297 40-41 22.05544122550779 27.189729100686556 29.363840898852338 21.390988774953318 42-43 21.934377616519953 27.27492687725373 29.152496312021558 21.638199194204763 44-45 21.91774742675225 27.45844641725389 29.05876294038326 21.5650432156106 46-47 21.93289332171823 27.39540631799412 28.796918648652863 21.874781711634792 48-49 21.906496149715384 27.52259111948168 28.775872093379874 21.79504063742306 50-51 22.00009366707388 27.690801380990386 28.566624603078562 21.74248034885717 52-53 22.012797000024822 27.747223169912967 28.440001939639124 21.79997789042309 54-55 21.910670895514958 27.7355741207069 28.523778468346634 21.82997651543151 56-57 21.9998888762211 27.786919432373963 28.274507483537086 21.938684207867855 58-59 21.956866375674412 27.65285571926776 28.552668322950893 21.837609582106936 60-61 22.131990237655685 27.811330233789256 28.443251592954894 21.613427935600164 62-63 21.980307913026316 27.781632674416635 28.544065076909696 21.693994335647353 64-65 21.878417413568275 27.56644399065943 28.49825710329536 22.056881492476936 66-67 21.870675804059147 27.651855387882744 28.363340885285027 22.114127922773086 68-69 21.752443724039797 27.720080216340776 28.43330679805993 22.094169261559497 70-71 21.82776750170678 27.6027879105298 28.3886457597671 22.180798827996323 72-73 21.76258304931616 27.89039041747819 28.135206848452093 22.211819684753564 74-75 21.789192257689106 27.904779092276762 28.076848725709848 22.229179924324285 76-77 21.68666883093502 27.800758196264407 28.216440593467496 22.29613237933308 78-79 21.658670955228516 27.735132620373875 28.14750451321535 22.45869191118226 80-81 21.641039170287105 27.981104210435316 27.933954216157687 22.443902403119896 82-83 21.817024362654315 27.94332847902267 27.9725135213565 22.267133636966506 84-85 21.700989627694852 27.985990799001353 27.89168727458845 22.421332298715342 86-87 21.54365943378306 27.974867649257973 28.039231479412525 22.442241437546443 88-89 21.48671379938765 27.838819749206234 28.070824227918962 22.603642223487157 90-91 21.735484067467887 27.896470694883323 27.90905066211911 22.458994575529676 92-93 21.594737022939665 27.85402501590165 27.86662795797402 22.68461000318466 94-95 21.427440202045926 27.757963267579477 27.903955423776434 22.910641106598167 96-97 21.453477269213764 27.736583885780885 27.932591078244528 22.87734776676082 98-99 21.330361286818388 27.718733700913113 27.860496740056323 23.09040827221217 100 20.446523448990792 28.223588509522525 28.081312890601207 23.248575150885483 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3132.0 1 2295.5 2 2370.5 3 3401.0 4 2716.5 5 2216.5 6 3339.0 7 6591.0 8 10440.0 9 12297.5 10 10474.0 11 7888.0 12 7889.0 13 8518.0 14 9038.5 15 9467.0 16 10008.0 17 10617.5 18 11299.5 19 12260.5 20 13539.5 21 15359.0 22 18000.0 23 22079.0 24 27441.5 25 33985.0 26 42513.5 27 52331.5 28 62749.5 29 74754.5 30 89186.0 31 103410.5 32 119121.0 33 136165.5 34 152949.5 35 168380.0 36 187123.5 37 205292.5 38 216762.0 39 226321.0 40 229851.5 41 230361.5 42 233222.0 43 237916.0 44 241934.5 45 248105.5 46 252940.5 47 253443.0 48 256247.0 49 258484.0 50 256857.5 51 248361.0 52 235589.5 53 224066.5 54 211679.0 55 194757.5 56 174452.0 57 154198.0 58 135008.5 59 115176.0 60 94481.0 61 77477.5 62 63281.5 63 48959.5 64 36065.5 65 26267.0 66 19127.0 67 14362.0 68 10989.0 69 8347.5 70 6445.5 71 4867.5 72 3662.0 73 2837.0 74 2222.5 75 1649.5 76 1140.0 77 740.5 78 484.5 79 335.0 80 236.0 81 164.5 82 110.0 83 83.0 84 66.5 85 51.0 86 35.0 87 29.5 88 22.5 89 13.5 90 11.5 91 5.5 92 3.0 93 2.0 94 2.0 95 4.0 96 3.0 97 2.0 98 4.0 99 3.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0033191878207165233 2 0.06827425034699944 3 7.215625697209833E-4 4 7.504250725098227E-4 5 0.0016307314075694223 6 6.349750613544653E-4 7 0.0 8 0.0010679126031870553 9 7.215625697209832E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 8.6587508366518E-4 18-19 0.001082343854581475 20-21 0.003153228429680697 22-23 7.215625697209833E-6 24-25 2.8862502788839333E-5 26-27 1.6595939103582617E-4 28-29 9.668938434261177E-4 30-31 0.0016523782846610518 32-33 0.002972837787250451 34-35 0.0043438066697203195 36-37 0.0014792032679280158 38-39 0.0016307314075694223 40-41 0.005664266172309719 42-43 0.006847628786652131 44-45 0.005346778641632487 46-47 0.007186763194420994 48-49 0.009127766506970439 50-51 0.008810278976293206 52-53 0.0040696128932263455 54-55 0.0025254689940234416 56-57 0.0028285252733062545 58-59 0.0034779315860551394 60-61 0.00775679762450057 62-63 0.014265292003383839 64-65 0.004047966016134716 66-67 0.010585322897806825 68-69 0.012879891869519553 70-71 0.006025047457170211 72-73 0.007807307004381038 74-75 0.0033841284519914114 76-77 0.002850172150397884 78-79 0.008478360194221553 80-81 0.006566219384460948 82-83 0.01731028604760639 84-85 0.017966907986052487 86-87 0.023118864733860305 88-89 0.02197158024800394 90-91 0.02489390865537392 92-93 0.03538542841911702 94-95 0.03844485371473399 96-97 0.023075570979677044 98-99 0.018695686181470675 100 0.018818351818323243 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6929406.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.54903741550002 #Duplication Level Percentage of deduplicated Percentage of total 1 72.5733184641418 41.76524619659895 2 16.496887214583822 18.987599591027365 3 5.335362911356765 9.211349994328248 4 2.1909497730241596 5.043482018529946 5 1.1086020141586546 3.189948939585755 6 0.6048774598069944 2.08860693397352 7 0.38347690270535767 1.5448108635239484 8 0.24078521915797585 1.108556606913738 9 0.18126574732307194 0.9388502347359634 >10 0.8058235855074607 7.997215714178059 >50 0.04655526905716734 1.848864710144435 >100 0.029317285274927753 3.1651476537335554 >500 0.0018164865170358076 0.7302831897403034 >1k 8.54815453101767E-4 0.9009435710912314 >5k 0.0 0.0 >10k+ 1.0685193163614281E-4 1.4790937818947847 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28037 0.4046089953453442 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26265 0.37903681787443255 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17858 0.2577132874015464 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 2.8862502788839333E-5 7 0.0 0.0 0.0 1.4431251394419667E-5 2.8862502788839333E-5 8 0.0 0.0 0.0 1.4431251394419667E-5 2.8862502788839333E-5 9 0.0 0.0 0.0 1.4431251394419667E-5 4.3293754183258996E-5 10-11 0.0 0.0 0.0 2.8862502788839333E-5 4.3293754183258996E-5 12-13 0.0 0.0 0.0 2.8862502788839333E-5 4.3293754183258996E-5 14-15 0.0 0.0 0.0 2.8862502788839333E-5 4.3293754183258996E-5 16-17 0.0 0.0 0.0 5.7725005577678666E-5 4.3293754183258996E-5 18-19 0.0 0.0 0.0 8.658750836651799E-5 7.215625697209834E-5 20-21 0.0 0.0 0.0 9.380313406372783E-5 7.937188266930816E-5 22-23 0.0 0.0 0.0 1.515281396414065E-4 1.2266563685256717E-4 24-25 0.0 4.3293754183258996E-5 0.0 3.102719049800228E-4 1.4431251394419667E-4 26-27 0.0 4.3293754183258996E-5 0.0 4.4736879322700965E-4 1.5152813964140651E-4 28-29 0.0 5.050937988046883E-5 0.0 0.0012843813741033503 1.5874376533861633E-4 30-31 0.0 5.7725005577678666E-5 0.0 0.004480903557967306 1.5874376533861633E-4 32-33 7.215625697209833E-6 5.7725005577678666E-5 0.0 0.01208617304282647 1.5874376533861633E-4 34-35 1.4431251394419667E-5 5.7725005577678666E-5 0.0 0.024064111700194794 1.5874376533861633E-4 36-37 1.4431251394419667E-5 6.49406312748885E-5 0.0 0.044433823043418155 1.5874376533861633E-4 38-39 1.4431251394419667E-5 7.937188266930816E-5 0.0 0.07588673545755581 1.6595939103582614E-4 40-41 1.4431251394419667E-5 9.380313406372783E-5 0.0 0.11241223273683199 1.8760626812745567E-4 42-43 1.4431251394419667E-5 1.0101875976093766E-4 0.0 0.14900267064738304 1.8760626812745567E-4 44-45 1.4431251394419667E-5 1.0101875976093766E-4 0.0 0.19443225003701617 1.948218938246655E-4 46-47 1.4431251394419667E-5 1.0101875976093766E-4 0.0 0.2511687148941771 2.1646877091629498E-4 48-49 2.1646877091629498E-5 1.0101875976093766E-4 0.0 0.311137780063688 2.3090002231071467E-4 50-51 2.8862502788839333E-5 1.0101875976093766E-4 0.0 0.3779256115170622 2.3090002231071467E-4 52-53 2.8862502788839333E-5 1.0101875976093766E-4 0.0 0.4440063116521099 2.453312737051343E-4 54-55 2.8862502788839333E-5 1.29881262549777E-4 0.0 0.522108244198709 2.59762525099554E-4 56-57 2.8862502788839333E-5 1.29881262549777E-4 0.0 0.6135518686594492 2.59762525099554E-4 58-59 2.8862502788839333E-5 1.29881262549777E-4 0.0 0.7191958444922985 2.59762525099554E-4 60-61 2.8862502788839333E-5 1.29881262549777E-4 0.0 0.8333759055249468 2.59762525099554E-4 62-63 4.3293754183258996E-5 1.29881262549777E-4 0.0 0.951019466892256 2.8862502788839335E-4 64-65 4.3293754183258996E-5 1.29881262549777E-4 0.0 1.074644781962552 2.958406535856032E-4 66-67 4.3293754183258996E-5 1.29881262549777E-4 0.0 1.2207612023310512 3.03056279282813E-4 68-69 4.3293754183258996E-5 1.29881262549777E-4 0.0 1.389253277986598 3.03056279282813E-4 70-71 4.3293754183258996E-5 1.29881262549777E-4 0.0 1.5736846708072814 3.1748753067723266E-4 72-73 4.3293754183258996E-5 1.29881262549777E-4 0.0 1.7609085107727849 3.7521253625491134E-4 74-75 4.3293754183258996E-5 1.29881262549777E-4 0.0 1.9520576511175705 3.7521253625491134E-4 76-77 4.3293754183258996E-5 1.29881262549777E-4 0.0 2.1685913626651407 3.824281619521212E-4 78-79 4.3293754183258996E-5 1.29881262549777E-4 0.0 2.412241395582825 3.8964378764933097E-4 80-81 4.3293754183258996E-5 1.29881262549777E-4 0.0 2.68353449054652 4.401531675297998E-4 82-83 4.3293754183258996E-5 1.29881262549777E-4 0.0 2.9656510240560303 5.123094245018981E-4 84-85 4.3293754183258996E-5 1.29881262549777E-4 0.0 3.246100748029485 5.19525050199108E-4 86-87 4.3293754183258996E-5 1.29881262549777E-4 0.0 3.5396612638947698 5.483875529879473E-4 88 4.3293754183258996E-5 1.29881262549777E-4 0.0 3.7797179152152434 5.483875529879473E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13825 0.0 52.073124 1 GTATCAA 23145 0.0 36.142845 1 TACCTGG 5835 0.0 29.736235 2 TCAACGC 28795 0.0 28.493065 4 ATCAACG 28815 0.0 28.456984 3 CAACGCA 29245 0.0 28.10284 5 GTACCTG 6450 0.0 27.63014 1 TATCAAC 30110 0.0 27.422953 2 AACGCAG 30345 0.0 27.130182 6 GTACATG 26255 0.0 26.094627 1 TACATGG 27090 0.0 24.942974 2 ACATGGG 26760 0.0 24.145079 3 ACGCAGA 34170 0.0 23.996613 7 CGCAGAG 34505 0.0 23.613838 8 ACCTGGG 7400 0.0 22.92385 3 CATGGGG 15080 0.0 22.716333 4 TATAACG 615 0.0 22.172943 2 ATGGGGG 8970 0.0 21.111769 5 GAGTACT 23235 0.0 21.063711 12-13 GCAGAGT 38170 0.0 21.0138 9 >>END_MODULE