FastQCFastQC Report
Mon 6 Jun 2016
ERR1211533_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211533_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4085668
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160280.39229815051051625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143230.35056690852022243No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92580.22659697263703268No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54580.1335889259724481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74750.049.998241
TACCTGG42550.041.222482
GTATCAA124150.037.0798151
GTACCTG48600.036.6101041
ACCTGGG52900.032.780353
TATAACG4400.029.9247262
TCAACGC150750.029.7688714
ATCAACG151900.029.5762443
CAACGCA153150.029.2987795
GTACATG136250.029.0193861
TATCAAC157100.028.7655092
AACGCAG160050.028.1468896
ATAACGC5050.027.917263
TACATGG139450.027.5167082
ACATGGG139200.026.6704773
CATGGGG90950.026.4535274
ATGGGGG58500.025.942385
TAACGCA5450.025.8667054
ACGCAGA178800.025.143937
CGCAGAG183850.024.3008548
Produced by FastQC (version 0.11.5)