##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211533_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4085668 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.309916517935378 31.0 31.0 34.0 28.0 34.0 2 31.340269938722383 31.0 31.0 34.0 28.0 34.0 3 31.430024906575866 33.0 31.0 34.0 28.0 34.0 4 34.97939847290578 37.0 35.0 37.0 32.0 37.0 5 35.06947701085844 37.0 35.0 37.0 32.0 37.0 6 35.18661403716602 37.0 35.0 37.0 32.0 37.0 7 35.12015929830813 37.0 35.0 37.0 32.0 37.0 8 35.08984528356196 37.0 35.0 37.0 32.0 37.0 9 36.65704286300306 39.0 37.0 39.0 32.0 39.0 10-11 36.63965855767037 39.0 37.0 39.0 32.0 39.0 12-13 36.57818782632363 39.0 37.0 39.0 32.0 39.0 14-15 37.753809046647945 40.0 38.0 41.0 32.0 41.0 16-17 37.73063682609551 40.0 37.5 41.0 32.0 41.0 18-19 37.745328426098254 40.0 38.0 41.0 32.0 41.0 20-21 37.7023235857637 40.0 37.5 41.0 32.0 41.0 22-23 37.553392370598885 40.0 37.0 41.0 32.0 41.0 24-25 37.467879303947356 40.0 37.0 41.0 31.0 41.0 26-27 37.31967881384391 40.0 37.0 41.0 31.0 41.0 28-29 37.15173222591753 40.0 36.0 41.0 30.5 41.0 30-31 36.964475454197455 39.5 36.0 41.0 30.0 41.0 32-33 36.78003964100852 39.0 36.0 41.0 30.0 41.0 34-35 36.60268222968679 39.0 35.0 41.0 30.0 41.0 36-37 36.431035022914244 39.0 35.0 41.0 29.5 41.0 38-39 36.29616699154214 39.0 35.0 40.0 29.0 41.0 40-41 36.25149277914897 39.0 35.0 40.0 28.5 41.0 42-43 36.18482032803449 39.0 35.0 40.0 28.0 41.0 44-45 36.000846128466634 39.0 35.0 40.0 27.5 41.0 46-47 35.93801454254237 39.0 35.0 40.0 27.0 41.0 48-49 35.89814015725213 39.0 35.0 41.0 27.0 41.0 50-51 34.91127913477062 38.0 34.0 40.0 25.0 40.5 52-53 35.116131193234494 38.0 34.0 39.5 26.0 40.5 54-55 35.70059559415988 39.0 35.0 40.5 26.5 41.0 56-57 35.65931213696267 39.0 35.0 41.0 26.0 41.0 58-59 35.49787929415704 38.5 34.0 40.5 26.0 41.0 60-61 35.30274045761917 38.0 34.0 40.0 26.0 41.0 62-63 35.037151574724135 38.0 34.0 40.0 26.0 41.0 64-65 34.75115207598856 37.0 34.0 40.0 25.5 41.0 66-67 34.44227822720789 37.0 33.5 40.0 25.0 41.0 68-69 34.152918200891506 36.0 33.0 39.0 25.0 41.0 70-71 33.80491280740383 36.0 33.0 39.0 24.5 41.0 72-73 33.45453534158918 35.0 33.0 38.5 24.0 40.0 74-75 33.092550961066834 35.0 32.0 37.5 23.5 40.0 76-77 32.755399239487886 35.0 32.0 37.0 23.0 39.0 78-79 32.43984912626283 35.0 32.0 36.5 23.0 39.0 80-81 32.109057074632595 35.0 31.5 36.0 22.0 38.5 82-83 31.817678161808548 35.0 31.0 36.0 21.0 37.0 84-85 31.56204126228563 35.0 31.0 35.0 20.5 37.0 86-87 31.276016553474243 34.5 31.0 35.0 20.0 36.5 88-89 31.062942583685214 34.0 31.0 35.0 20.0 36.0 90-91 30.84101290168462 34.0 30.0 35.0 19.5 36.0 92-93 30.69061154748746 34.0 30.0 35.0 19.0 35.5 94-95 30.53043235524766 34.0 30.0 35.0 18.5 35.0 96-97 30.431830853608272 34.0 30.0 35.0 18.0 35.0 98-99 30.25688553254939 34.0 29.5 35.0 18.0 35.0 100 28.87395280282196 33.0 27.0 35.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.468425319809306 1101 2 0.42505757270440014 1101 3 0.3801246247327157 1101 4 0.29641520274550714 1101 5 0.2757929350875372 1101 6 0.32360980569877995 1101 7 0.3710613211214877 1101 8 0.3646784899523752 1101 9 0.42873903787656786 1101 10-11 0.4041563932179457 1101 12-13 0.35101826581342976 1101 14-15 0.4464139517154777 1101 16-17 0.43063270137383114 1101 18-19 0.3731039659569575 1101 20-21 0.405491631624308 1101 22-23 0.4645246196032886 1101 24-25 0.4469292602594521 1101 26-27 0.4457147883208492 1101 28-29 0.4348166417469699 1101 30-31 0.41971330393516837 1101 32-33 0.33771569733290363 1101 34-35 0.36787829920930193 1101 36-37 0.3624730122108275 1101 38-39 0.33119356041212455 1101 40-41 0.32172014532717697 1101 42-43 0.3096991327749308 1101 44-45 0.2713559588839729 1101 46-47 0.11849642049057252 1101 48-49 0.24936696614000198 1101 50-51 0.25853129229295035 1101 52-53 0.47559835763023983 1101 54-55 0.4740624747927953 1101 56-57 0.343035766713669 1101 58-59 0.3592684827610313 1101 60-61 0.3488003130360653 1101 62-63 0.35860426904404363 1101 64-65 0.3421610795307046 1101 66-67 0.3245708276586754 1101 68-69 0.19648900363371524 1101 70-71 0.2454740297181388 1101 72-73 0.20134879152045215 1101 74-75 0.22850070655677257 1101 76-77 0.09453888296422264 1101 78-79 0.045712257616372654 1101 80-81 -0.03973635322874003 1101 82-83 -0.021960148450673955 1101 84-85 -0.1895947978610728 1101 86-87 -0.10928183476485387 1101 88-89 -0.07532110756783439 1101 90-91 -0.23087030658664887 1101 92-93 -0.13375997757404434 1101 94-95 -0.23740489568651668 1101 96-97 -0.32922739549550784 1101 98-99 -0.2900377143981956 1101 100 -0.21324051740593575 1102 1 0.2903611467407252 1102 2 0.3061164681062891 1102 3 0.326197564943957 1102 4 0.2994097593692189 1102 5 0.28846852282266866 1102 6 0.2904093346416161 1102 7 0.26736043652681474 1102 8 0.29774608032118266 1102 9 0.31534883669037583 1102 10-11 0.27846915867552013 1102 12-13 0.29621662757148215 1102 14-15 0.33259741654858743 1102 16-17 0.31363173399800814 1102 18-19 0.3716066876450981 1102 20-21 0.35710903098519253 1102 22-23 0.32922954235683477 1102 24-25 0.33855734420719585 1102 26-27 0.3456981805363242 1102 28-29 0.24471058207825536 1102 30-31 0.257052206584099 1102 32-33 0.32714591039126617 1102 34-35 0.4051506694531568 1102 36-37 0.355894755299083 1102 38-39 0.2977287825403181 1102 40-41 0.32658970609362825 1102 42-43 0.1845232504449541 1102 44-45 0.08184622362899319 1102 46-47 0.19228359191605193 1102 48-49 0.280616390759441 1102 50-51 0.2800716072295657 1102 52-53 0.3179517248861643 1102 54-55 0.3166727839921819 1102 56-57 0.39970320300938056 1102 58-59 0.39270076313874114 1102 60-61 0.26910076111482795 1102 62-63 0.32666033707189257 1102 64-65 0.32902956023879426 1102 66-67 0.2453168964745558 1102 68-69 0.24712626752515376 1102 70-71 0.2757823158610577 1102 72-73 0.2379218212148757 1102 74-75 0.22778797114786897 1102 76-77 0.12058166586917451 1102 78-79 0.07381398232190861 1102 80-81 0.07848050834924436 1102 82-83 0.10991505086115083 1102 84-85 0.0902223434347178 1102 86-87 -0.03780465518646281 1102 88-89 8.314303299528092E-4 1102 90-91 -0.1974434117710011 1102 92-93 -0.14754384033114576 1102 94-95 -0.07614574759590198 1102 96-97 -0.22051480812051594 1102 98-99 -0.3310505185949957 1102 100 -0.17272498010544268 1103 1 0.32448810989551546 1103 2 0.35318253191284654 1103 3 0.27800626116857075 1103 4 0.36529688952979456 1103 5 0.2799340468181626 1103 6 0.27241309811313386 1103 7 0.31778214653737535 1103 8 0.3287153990809131 1103 9 0.3127087971178355 1103 10-11 0.3654177519169437 1103 12-13 0.26838399867211393 1103 14-15 0.40672139414577657 1103 16-17 0.40504276229543734 1103 18-19 0.3551853327489951 1103 20-21 0.34630444175665076 1103 22-23 0.4069880190919761 1103 24-25 0.4351991250459477 1103 26-27 0.4399123182641773 1103 28-29 0.4173971805870522 1103 30-31 0.3998672582449032 1103 32-33 0.4155455588001331 1103 34-35 0.33536754888717013 1103 36-37 0.37172497906291113 1103 38-39 0.3364371162792992 1103 40-41 0.2011801770653392 1103 42-43 0.21857385935498996 1103 44-45 0.2832932918717077 1103 46-47 0.25109012917455686 1103 48-49 0.3350368240268935 1103 50-51 0.3839590674704283 1103 52-53 0.31705791199068756 1103 54-55 0.3975194352767275 1103 56-57 0.3802055416188921 1103 58-59 0.3743475577671447 1103 60-61 0.279994731632911 1103 62-63 0.29402052002537715 1103 64-65 0.39143128370291436 1103 66-67 0.35743427339332356 1103 68-69 0.35796168588104393 1103 70-71 0.3660731573849745 1103 72-73 0.2996186702926167 1103 74-75 0.328379310898292 1103 76-77 0.3100327497795732 1103 78-79 0.1721314073619311 1103 80-81 0.12014359373758055 1103 82-83 0.1220335635898735 1103 84-85 0.10197487038007225 1103 86-87 0.19547870250883648 1103 88-89 0.2001525173139882 1103 90-91 0.1626013901367891 1103 92-93 0.04005909534353691 1103 94-95 0.02678434075157199 1103 96-97 0.10967128854571584 1103 98-99 0.07233329105994457 1103 100 0.03662032512841762 1104 1 0.35206265661296854 1104 2 0.3867143041598382 1104 3 0.37257876183863203 1104 4 0.39900325530418 1104 5 0.2809879310994532 1104 6 0.3239115352651254 1104 7 0.2907802665897776 1104 8 0.2990221363148251 1104 9 0.3867697670650969 1104 10-11 0.4330266652468211 1104 12-13 0.38814805675419706 1104 14-15 0.46802172426910715 1104 16-17 0.40413428610999347 1104 18-19 0.35229774455567053 1104 20-21 0.39584153297186475 1104 22-23 0.5203380300394613 1104 24-25 0.47996077656859626 1104 26-27 0.5056999379787186 1104 28-29 0.460388878428887 1104 30-31 0.37628130066074306 1104 32-33 0.2929101685977571 1104 34-35 0.3225585183436763 1104 36-37 0.4527623052715697 1104 38-39 0.46932010841598526 1104 40-41 0.4207504127382862 1104 42-43 0.21585730203734954 1104 44-45 0.2965254521881988 1104 46-47 0.3522528748795608 1104 48-49 0.31652934387393117 1104 50-51 0.37465257183621503 1104 52-53 0.5103248069816502 1104 54-55 0.4907035163107807 1104 56-57 0.4640542483055441 1104 58-59 0.5355139699362184 1104 60-61 0.5524310380266186 1104 62-63 0.5373108199864944 1104 64-65 0.3983549315940067 1104 66-67 0.35829369823505175 1104 68-69 0.21238621617727205 1104 70-71 0.2535984262590105 1104 72-73 0.39127968233108135 1104 74-75 0.4573481520880236 1104 76-77 0.4570879468154416 1104 78-79 0.29281040630870336 1104 80-81 0.22499198521212804 1104 82-83 0.27504013491274293 1104 84-85 0.07579603375808475 1104 86-87 0.16498509357568736 1104 88-89 0.28954865020891773 1104 90-91 0.24057426962761852 1104 92-93 0.30907407641193174 1104 94-95 0.2709070979325574 1104 96-97 0.2178371693842216 1104 98-99 0.1348722914789704 1104 100 0.1414675133232528 1105 1 0.34321573681136996 1105 2 0.42420686716265976 1105 3 0.4294312071543729 1105 4 0.435433667535186 1105 5 0.33877689436263125 1105 6 0.36398326053973307 1105 7 0.3450064615967747 1105 8 0.3735472558893136 1105 9 0.4727365999994575 1105 10-11 0.47047267420074235 1105 12-13 0.4246759946205074 1105 14-15 0.5213180519070804 1105 16-17 0.5219315155081503 1105 18-19 0.563625941765423 1105 20-21 0.5902778435377556 1105 22-23 0.6925229130125743 1105 24-25 0.596753622992857 1105 26-27 0.5581447003132567 1105 28-29 0.618233658006389 1105 30-31 0.6850776081042724 1105 32-33 0.6118104522522074 1105 34-35 0.6305742808941446 1105 36-37 0.6154050160046225 1105 38-39 0.6146663151979013 1105 40-41 0.6553892271061841 1105 42-43 0.779345519736701 1105 44-45 0.6110066449349745 1105 46-47 0.5986503973303599 1105 48-49 0.5120586794296926 1105 50-51 0.5565261824963912 1105 52-53 0.5650916406150515 1105 54-55 0.7146535890108225 1105 56-57 0.6803595539331155 1105 58-59 0.7092331462043191 1105 60-61 0.706224644461507 1105 62-63 0.6592281069854593 1105 64-65 0.6688872089384716 1105 66-67 0.636671085258719 1105 68-69 0.626819874278894 1105 70-71 0.525507033524967 1105 72-73 0.6252602836132723 1105 74-75 0.5951514378815332 1105 76-77 0.5671349707738358 1105 78-79 0.5092030432543027 1105 80-81 0.5168980565203114 1105 82-83 0.489260071579821 1105 84-85 0.4624150311498916 1105 86-87 0.500592795275594 1105 88-89 0.6383797406629981 1105 90-91 0.4764632471045829 1105 92-93 0.4828847588137073 1105 94-95 0.3825967176709284 1105 96-97 0.42496627876206006 1105 98-99 0.47330639191688206 1105 100 0.43691999343559473 1106 1 0.2729997706236098 1106 2 0.2590724430934195 1106 3 0.22252673845614623 1106 4 0.17032427539859896 1106 5 0.20261155127268893 1106 6 0.19334559796420336 1106 7 0.1781642458801045 1106 8 0.2435901709057049 1106 9 0.2676504341481589 1106 10-11 0.27975739868306704 1106 12-13 0.24452998431397077 1106 14-15 0.26856807686926487 1106 16-17 0.2776039088937452 1106 18-19 0.23711053825803674 1106 20-21 0.24795241286793868 1106 22-23 0.3325332785445667 1106 24-25 0.366905175799765 1106 26-27 0.33571261089026905 1106 28-29 0.33116387691023874 1106 30-31 0.45635549823122545 1106 32-33 0.40441864809758243 1106 34-35 0.3373452692030767 1106 36-37 0.45154266652256325 1106 38-39 0.45088597718311263 1106 40-41 0.3838887700143232 1106 42-43 0.37130757882042786 1106 44-45 0.38843170807782457 1106 46-47 0.4313008664499023 1106 48-49 0.4984780089445948 1106 50-51 0.4457392530715012 1106 52-53 0.5279712376168746 1106 54-55 0.5351268998087733 1106 56-57 0.4741414205699215 1106 58-59 0.4160351753055309 1106 60-61 0.37417541007791044 1106 62-63 0.44837278816812187 1106 64-65 0.5273554459868919 1106 66-67 0.6034154352864505 1106 68-69 0.550319069410584 1106 70-71 0.5481372658416319 1106 72-73 0.46114068917044193 1106 74-75 0.5358117567476981 1106 76-77 0.49035599456792056 1106 78-79 0.46453848211331206 1106 80-81 0.4766322696118124 1106 82-83 0.4502387563709931 1106 84-85 0.42677357492119583 1106 86-87 0.4715641239470614 1106 88-89 0.4789322587937086 1106 90-91 0.5003864305605497 1106 92-93 0.5360423398122407 1106 94-95 0.575779013716982 1106 96-97 0.47026077367007346 1106 98-99 0.49064150352721114 1106 100 0.6162846526914016 1107 1 0.29377527321026875 1107 2 0.31928524163166827 1107 3 0.26255302573317607 1107 4 0.2672393685306105 1107 5 0.2947981646204241 1107 6 0.22952619458809664 1107 7 0.25979786536140637 1107 8 0.313986298477154 1107 9 0.24286165552953776 1107 10-11 0.3886721931322725 1107 12-13 0.358106962130492 1107 14-15 0.36521474428297296 1107 16-17 0.37373341440820695 1107 18-19 0.3386285467575334 1107 20-21 0.5043217040485857 1107 22-23 0.4633713509917001 1107 24-25 0.41591278323884495 1107 26-27 0.47489829947774354 1107 28-29 0.4334711617001119 1107 30-31 0.5068400702076019 1107 32-33 0.4134990141096324 1107 34-35 0.5458134052509038 1107 36-37 0.5956822925589336 1107 38-39 0.528981910192023 1107 40-41 0.5116116087212177 1107 42-43 0.5850784312866821 1107 44-45 0.5065686332081327 1107 46-47 0.43864735684719136 1107 48-49 0.5240546681395415 1107 50-51 0.500413309977553 1107 52-53 0.4625761528432051 1107 54-55 0.5582752658064649 1107 56-57 0.5740140317554889 1107 58-59 0.5397130851798906 1107 60-61 0.6300616845064013 1107 62-63 0.5512565527039825 1107 64-65 0.5422810125570621 1107 66-67 0.5517288544389203 1107 68-69 0.6238179191428301 1107 70-71 0.6553296494699836 1107 72-73 0.6169243088311873 1107 74-75 0.5291023101164143 1107 76-77 0.5436251157505936 1107 78-79 0.5189848880898396 1107 80-81 0.5684647703684931 1107 82-83 0.607250654235024 1107 84-85 0.5529606795602753 1107 86-87 0.5105216431969488 1107 88-89 0.4995205363111097 1107 90-91 0.5032448529171738 1107 92-93 0.5280017309140419 1107 94-95 0.5024226763577424 1107 96-97 0.4129661141106489 1107 98-99 0.4913541650860971 1107 100 0.6750064298580796 1108 1 0.3234710302119872 1108 2 0.293003393588652 1108 3 0.34538109761233926 1108 4 0.2562373536075597 1108 5 0.25242827809660895 1108 6 0.26994804264707994 1108 7 0.2291429634601272 1108 8 0.21539103446473717 1108 9 0.2576888696887849 1108 10-11 0.28400440786253256 1108 12-13 0.246641107609058 1108 14-15 0.3412139600362636 1108 16-17 0.3583946829232616 1108 18-19 0.38726182517230257 1108 20-21 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2316 94-95 0.15240585054737465 2316 96-97 0.04278869719452061 2316 98-99 0.32978482568941914 2316 100 0.18378941202958288 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 9.0 1 30.0 2 56.0 3 70.0 4 60.0 5 74.0 6 175.0 7 1687.0 8 2850.0 9 3180.0 10 4302.0 11 6193.0 12 8514.0 13 10151.0 14 11289.0 15 12465.0 16 14457.0 17 17372.0 18 20947.0 19 25389.0 20 29630.0 21 34740.0 22 39900.0 23 42779.0 24 42625.0 25 43072.0 26 46853.0 27 52184.0 28 60097.0 29 71147.0 30 85953.0 31 105670.0 32 132878.0 33 173589.0 34 241503.0 35 340271.0 36 516442.0 37 823694.0 38 853295.0 39 209752.0 40 324.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.568011179416146 18.121931636399296 12.535950035855034 23.774107148329524 2 16.183497351679083 19.96837401573067 40.44499827757461 23.403130355015637 3 18.412433916905645 26.143381676954082 30.406608884789865 25.037575521350412 4 12.379170442239671 16.505511622480732 37.90377412031087 33.21154381496873 5 12.728418060104868 37.34499252184017 35.12585563050717 14.800733787547793 6 30.41556012992067 36.44299326654543 18.941812280783342 14.19963432275056 7 26.741741785844013 32.81601700449491 22.851821609454685 17.59041960020639 8 25.869710909821663 35.16717179406468 20.88593683223194 18.077180463881717 9 27.075790801633996 15.169542201420997 20.435042172905117 37.319624824039884 10-11 24.62740936963994 25.634891350042498 29.45658193371285 20.281117346604717 12-13 25.135281272695277 23.791145835500615 29.045936274669387 22.02763661713472 14-15 22.69810265808041 25.147185615197927 26.829997615654232 25.324714111067426 16-17 22.16010047616518 27.74324922705464 27.52531180337925 22.571338493400926 18-19 21.84200721471614 26.927743168639097 29.383383141769954 21.84686647487481 20-21 22.6897658057897 26.382620271568047 29.29151030993578 21.63610361270647 22-23 22.382128569028854 26.174527047509997 29.20335010360413 22.23999427985702 24-25 22.0260570331897 26.239636824515568 29.304474639710016 22.42983150258471 26-27 21.92382096561036 26.389705848155483 29.18565255524052 22.500820630993633 28-29 21.987225336721533 26.800890522244757 29.14439330754441 22.067490833489305 30-31 22.679271300521155 26.39973209685981 29.18657101238575 21.734425590233286 32-33 22.29369117724461 26.436624735148424 29.16235294693666 22.107331140670308 34-35 22.42455177225532 26.72251956167167 28.981495591929914 21.871433074143095 36-37 22.391125849733942 26.718611988617337 28.89974265620318 21.990519505445544 38-39 22.31290082276611 26.9441028730594 28.745441419439842 21.99755488473465 40-41 22.423780750912968 26.53918748023834 28.950308529727835 22.086723239120854 42-43 22.211197421494305 26.787278666753505 28.589199030611923 22.412324881140268 44-45 21.985176392409603 27.04871330978382 28.746650780907025 22.219459516899555 46-47 22.025562379447276 26.694898760805525 28.459573970134873 22.819964889612322 48-49 22.09367607717795 26.82116275110428 28.476054804124374 22.609106367593398 50-51 22.14741517288176 27.143821080925147 28.468990262721817 22.23977348347128 52-53 22.174387287198563 27.21269488118593 28.15861232636545 22.45430550525006 54-55 22.075141766813864 27.006943743133526 28.48764723703901 22.430267253013596 56-57 22.20639147607346 27.11954922521554 28.09256482078534 22.581494477925656 58-59 22.16798474468425 27.195148911438032 28.27362423184202 22.363242112035692 60-61 22.48900011457704 27.228229628055512 28.284284730797822 21.998485526569635 62-63 22.32562177273491 27.232032003400946 28.30050989747686 22.141836326387278 64-65 22.216576069791504 26.95058312200047 28.245059120153442 22.58778168805458 66-67 22.097589940698683 27.015497513780605 28.191780463088122 22.69513208243259 68-69 21.950244990058277 27.097502823748055 28.322728543034142 22.629523643159526 70-71 22.013358465544336 26.898395250541014 28.36483674591736 22.723409537997288 72-73 21.886577442249987 27.37193369370305 27.963050861443424 22.778438002603547 74-75 22.067679427465794 27.306233713955226 27.9014318741984 22.724654984380578 76-77 21.987228135802347 27.103110097043647 28.01501189788384 22.894649869270165 78-79 21.959465992976686 27.031981914165726 28.12521037511949 22.883341717738094 80-81 22.077563283066592 27.196075051986146 27.82148786875646 22.9048737961908 82-83 22.234533096886203 27.288468110482565 27.898811138344094 22.578187654287138 84-85 22.20114876263092 27.404571500473573 27.6847852722589 22.709494464636613 86-87 21.92990584251808 27.417033687405677 27.864949750973455 22.788110719102782 88-89 21.801219527271307 27.1025658004817 28.080106860719127 23.01610781152787 90-91 22.322976442559934 27.07542391077351 27.960508034345228 22.64109161232133 92-93 22.14937576177555 27.118138450384045 27.945988101459168 22.78649768638124 94-95 22.028079194589797 27.025037485847182 27.888943970133724 23.057939349429297 96-97 21.95061738670228 26.949390985140713 27.933645555750847 23.16634607240616 98-99 21.92120894695361 26.92151066471299 27.88559988651006 23.27168050182334 100 22.082948699427273 26.952401707162394 27.806644025298908 23.15800556811142 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2306.0 1 1712.0 2 2600.5 3 3689.5 4 2398.0 5 1721.5 6 2413.5 7 4348.0 8 6804.0 9 7830.5 10 6380.5 11 4621.0 12 4556.0 13 4800.0 14 5081.0 15 5459.0 16 5721.0 17 6106.0 18 6584.5 19 7004.5 20 7619.5 21 8702.5 22 10281.5 23 12686.5 24 15621.5 25 19110.5 26 23523.5 27 28473.0 28 33989.5 29 40436.5 30 47640.0 31 54225.0 32 61073.5 33 68933.5 34 76759.5 35 84397.0 36 93779.0 37 102921.5 38 110173.5 39 116793.0 40 120826.0 41 123644.5 42 126619.0 43 130307.0 44 135837.0 45 141987.0 46 146626.0 47 150133.5 48 154259.0 49 159118.5 50 162529.5 51 160270.0 52 153465.5 53 146705.5 54 139367.5 55 129281.0 56 117866.0 57 106369.0 58 94373.5 59 79981.5 60 64459.0 61 52055.5 62 42124.5 63 32961.0 64 24967.5 65 18759.5 66 13973.5 67 10608.0 68 8241.5 69 6302.0 70 4950.5 71 3875.0 72 3016.5 73 2433.0 74 1972.5 75 1537.5 76 1166.5 77 879.5 78 697.5 79 551.0 80 439.0 81 374.0 82 321.0 83 270.5 84 222.0 85 172.0 86 131.0 87 110.0 88 91.5 89 64.0 90 53.5 91 48.0 92 32.0 93 21.0 94 17.0 95 13.5 96 13.0 97 17.0 98 13.5 99 8.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16504033122612996 2 0.10314102859067355 3 0.030031808751959287 4 0.03179406647823563 5 0.02709471254149872 6 0.002398628571876129 7 0.003916128280614088 8 0.005629434403382752 9 0.005507055394613561 10-11 0.004540261225336958 12-13 0.007122458310366873 14-15 0.016888303210148253 16-17 0.007856732362982014 18-19 0.016741448399625228 20-21 0.017744956271532587 22-23 0.045378136451615744 24-25 0.01445296093564137 26-27 0.009716893296273706 28-29 0.026886668226591096 30-31 0.01491800116896429 32-33 0.020388342860947095 34-35 0.020327153356562502 36-37 0.021049189508300724 38-39 0.019311407583778223 40-41 0.025785257147668387 42-43 0.03529410612903447 44-45 0.036885033243033945 46-47 0.020486246067962448 48-49 0.013155743442687953 50-51 0.012029856562011401 52-53 0.022113886884592676 54-55 0.025834208751176063 56-57 0.01933588338553206 58-59 0.025919874057314496 60-61 0.026433865894145096 62-63 0.05144813528656758 64-65 0.031182171434389675 66-67 0.0208411451933931 68-69 0.02727828105465251 70-71 0.04077668572189419 72-73 0.0396875125438484 74-75 0.04038507289383278 76-77 0.022224027992484952 78-79 0.01720648863294815 80-81 0.022664592424054032 82-83 0.018405802918886216 84-85 0.018381327117132376 86-87 0.02111037901268532 88-89 0.017732718370655667 90-91 0.02792688980112922 92-93 0.025687353940653038 94-95 0.019409310790793572 96-97 0.013363787757595577 98-99 0.017732718370655667 100 0.041559911378017005 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4085668.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.6050355966999 #Duplication Level Percentage of deduplicated Percentage of total 1 78.6251357123073 52.36829962913588 2 13.986580638473283 18.631534026032533 3 3.836905897157483 7.6667176158408585 4 1.4062667042149437 3.746577755707608 5 0.6758385289385881 2.250712463878798 6 0.37796508982928223 1.5104626957433531 7 0.24444544500752502 1.1396908296324146 8 0.16036113755778528 0.8544687420290862 9 0.1187341228997291 0.7117461434051457 >10 0.5250567922076598 6.040071567076834 >50 0.026468032164969728 1.193561284781606 >100 0.015102973717775402 1.827628975596175 >500 6.570724215004629E-4 0.30039837396599195 >1k 3.0663379232223945E-4 0.4432451484070575 >5k 8.760965494921127E-5 0.42935726724317125 >10k+ 8.760965494921127E-5 0.8855274815233414 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16028 0.39229815051051625 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14323 0.35056690852022243 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9258 0.22659697263703268 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5458 0.1335889259724481 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 2.4475801753838052E-5 8 0.0 0.0 0.0 0.0 2.4475801753838052E-5 9 0.0 0.0 0.0 0.0 2.4475801753838052E-5 10-11 0.0 0.0 0.0 0.0 2.4475801753838052E-5 12-13 0.0 0.0 0.0 0.0 2.4475801753838052E-5 14-15 1.2237900876919026E-5 0.0 0.0 1.2237900876919026E-5 6.118950438459513E-5 16-17 2.4475801753838052E-5 0.0 0.0 2.4475801753838052E-5 7.342740526151415E-5 18-19 2.4475801753838052E-5 0.0 0.0 2.4475801753838052E-5 1.468548105230283E-4 20-21 2.4475801753838052E-5 0.0 0.0 7.342740526151415E-5 1.468548105230283E-4 22-23 2.4475801753838052E-5 0.0 0.0 1.5909271139994733E-4 2.0804431490762342E-4 24-25 2.4475801753838052E-5 0.0 0.0 2.937096210460566E-4 2.5699591841529953E-4 26-27 2.4475801753838052E-5 0.0 0.0 5.629434403382752E-4 2.6923381929221856E-4 28-29 2.4475801753838052E-5 0.0 0.0 0.001933588338553206 2.6923381929221856E-4 30-31 2.4475801753838052E-5 0.0 0.0 0.006204615744597946 2.6923381929221856E-4 32-33 2.4475801753838052E-5 0.0 0.0 0.015627799419825596 2.6923381929221856E-4 34-35 2.4475801753838052E-5 0.0 0.0 0.03101084082211281 3.1818542279989467E-4 36-37 2.4475801753838052E-5 0.0 0.0 0.05257402216724413 3.1818542279989467E-4 38-39 2.4475801753838052E-5 0.0 0.0 0.08899401517695515 3.304233236768137E-4 40-41 2.4475801753838052E-5 0.0 0.0 0.13415186941278634 3.9161282806140883E-4 42-43 2.4475801753838052E-5 0.0 0.0 0.17609115571798784 4.2832653069216586E-4 44-45 2.4475801753838052E-5 0.0 0.0 0.2264134041238789 4.405644315690849E-4 46-47 2.4475801753838052E-5 0.0 0.0 0.2894630694417657 4.405644315690849E-4 48-49 4.8951603507676104E-5 0.0 0.0 0.3571508991920048 4.405644315690849E-4 50-51 4.8951603507676104E-5 0.0 0.0 0.4330258846289028 4.6504023332292295E-4 52-53 4.8951603507676104E-5 0.0 0.0 0.5119848210867843 4.89516035076761E-4 54-55 4.8951603507676104E-5 0.0 0.0 0.6050785330575074 5.139918368305991E-4 56-57 4.8951603507676104E-5 0.0 0.0 0.7100185330770881 5.139918368305991E-4 58-59 4.8951603507676104E-5 0.0 0.0 0.8363503838295231 5.262297377075181E-4 60-61 4.8951603507676104E-5 0.0 0.0 0.9651665284599728 5.384676385844371E-4 62-63 4.8951603507676104E-5 0.0 0.0 1.099820151808713 5.384676385844371E-4 64-65 4.8951603507676104E-5 0.0 0.0 1.2401521611643433 5.384676385844371E-4 66-67 4.8951603507676104E-5 0.0 0.0 1.4056575326237961 5.507055394613561E-4 68-69 4.8951603507676104E-5 0.0 0.0 1.5992244107940246 5.629434403382752E-4 70-71 4.8951603507676104E-5 0.0 0.0 1.8158842079189987 5.996571429690322E-4 72-73 4.8951603507676104E-5 0.0 0.0 2.0243078977538067 6.853224491074654E-4 74-75 4.8951603507676104E-5 0.0 0.0 2.2378837438577976 6.853224491074654E-4 76-77 4.8951603507676104E-5 0.0 0.0 2.480071802212025 6.853224491074654E-4 78-79 4.8951603507676104E-5 0.0 0.0 2.760576728211886 6.853224491074654E-4 80-81 6.118950438459513E-5 0.0 0.0 3.051545059461513 7.220361517382226E-4 82-83 7.342740526151415E-5 0.0 0.0 3.3559506058739967 7.832256561228177E-4 84-85 7.342740526151415E-5 0.0 0.0 3.6686901627836623 7.832256561228177E-4 86-87 7.342740526151415E-5 0.0 0.0 3.998366974506984 7.832256561228177E-4 88 7.342740526151415E-5 0.0 0.0 4.266058818288711 8.077014578766556E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7475 0.0 49.99824 1 TACCTGG 4255 0.0 41.22248 2 GTATCAA 12415 0.0 37.079815 1 GTACCTG 4860 0.0 36.610104 1 ACCTGGG 5290 0.0 32.78035 3 TATAACG 440 0.0 29.924726 2 TCAACGC 15075 0.0 29.768871 4 ATCAACG 15190 0.0 29.576244 3 CAACGCA 15315 0.0 29.298779 5 GTACATG 13625 0.0 29.019386 1 TATCAAC 15710 0.0 28.765509 2 AACGCAG 16005 0.0 28.146889 6 ATAACGC 505 0.0 27.91726 3 TACATGG 13945 0.0 27.516708 2 ACATGGG 13920 0.0 26.670477 3 CATGGGG 9095 0.0 26.453527 4 ATGGGGG 5850 0.0 25.94238 5 TAACGCA 545 0.0 25.866705 4 ACGCAGA 17880 0.0 25.14393 7 CGCAGAG 18385 0.0 24.300854 8 >>END_MODULE