FastQCFastQC Report
Mon 6 Jun 2016
ERR1211533_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211533_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4085668
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174560.427249595414997No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163080.3991513750015909No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111770.2735660362026479No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53670.1313616280128488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82850.048.8074261
TACCTGG41650.042.1078642
GTACCTG47000.037.7158161
GTATCAA136850.035.561131
ACCTGGG50150.034.4839973
TATAACG4450.028.5281182
ATCAACG172800.027.7666023
TCAACGC172700.027.7282564
CAACGCA174150.027.5783425
TAACGCA4500.027.1519854
TATCAAC179600.026.8602712
GTACATG145350.026.4940811
AACGCAG181750.026.4503466
TACATGG149750.024.9300422
CCTGGGG49750.024.3706654
ACATGGG149250.024.0243113
CATGGGG94700.023.4720974
ACGCAGA204050.023.2833067
ATGGGGG62450.022.8008445
CGCAGAG210300.022.546658
Produced by FastQC (version 0.11.5)