##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1211533_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4085668
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09216387626209	33.0	31.0	34.0	30.0	34.0
2	32.30813835093796	34.0	31.0	34.0	30.0	34.0
3	32.315748122461244	34.0	31.0	34.0	30.0	34.0
4	35.96449882858813	37.0	35.0	37.0	35.0	37.0
5	35.888103487605946	37.0	35.0	37.0	35.0	37.0
6	35.910369613977444	37.0	35.0	37.0	35.0	37.0
7	35.83615629072162	37.0	35.0	37.0	35.0	37.0
8	35.83111060419006	37.0	35.0	37.0	35.0	37.0
9	37.47383781550532	39.0	37.0	39.0	35.0	39.0
10-11	37.43528842774303	39.0	37.0	39.0	34.0	39.0
12-13	37.34285849951587	39.0	37.0	39.0	34.0	39.0
14-15	38.56516829071769	40.0	38.0	41.0	34.0	41.0
16-17	38.48772036788109	40.0	38.0	41.0	34.0	41.0
18-19	38.48013739736073	40.0	38.0	41.0	34.0	41.0
20-21	38.41971006944274	40.0	38.0	41.0	34.0	41.0
22-23	38.30262799131012	40.0	38.0	41.0	34.0	41.0
24-25	38.18620284369655	40.0	37.5	41.0	33.0	41.0
26-27	38.005159009493674	40.0	37.0	41.0	33.0	41.0
28-29	38.03334313997124	40.0	37.0	41.0	33.0	41.0
30-31	37.94054950133002	40.0	37.0	41.0	33.0	41.0
32-33	37.72802599232243	40.0	37.0	41.0	33.0	41.0
34-35	37.595180641207264	40.0	37.0	41.0	32.5	41.0
36-37	37.52961865721835	40.0	37.0	41.0	32.0	41.0
38-39	37.42502817164782	40.0	36.0	41.0	32.0	41.0
40-41	37.25215387055434	40.0	36.0	41.0	31.0	41.0
42-43	37.09209534401718	39.5	36.0	41.0	31.0	41.0
44-45	36.90157374510117	39.0	35.5	41.0	30.5	41.0
46-47	36.62786978775564	39.0	35.0	41.0	30.0	41.0
48-49	36.393674669601154	39.0	35.0	41.0	29.5	41.0
50-51	36.183032737853395	39.0	35.0	40.0	28.5	41.0
52-53	35.981721104113205	39.0	35.0	40.0	28.0	41.0
54-55	35.77836549616856	38.0	35.0	40.0	27.5	41.0
56-57	35.57818758156561	38.0	34.0	40.0	27.0	41.0
58-59	35.317125498204945	38.0	34.0	40.0	26.5	41.0
60-61	34.99037391192823	38.0	34.0	40.0	26.0	41.0
62-63	34.652359051200435	37.0	33.0	40.0	26.0	41.0
64-65	34.3315310984642	36.5	33.0	39.5	25.5	41.0
66-67	33.98960414796308	36.0	32.5	39.0	25.0	40.5
68-69	33.58671262569548	35.5	32.0	39.0	23.5	40.0
70-71	33.19685801685306	35.0	32.0	38.5	23.5	40.0
72-73	32.785734058665554	35.0	31.0	37.5	22.5	40.0
74-75	32.372755813737186	35.0	31.0	37.0	22.0	39.0
76-77	30.826136019862602	33.5	29.5	35.5	20.5	37.5
78-79	31.66919142720358	34.0	30.5	36.0	21.0	38.0
80-81	31.65315353572537	34.0	31.0	36.0	21.0	37.5
82-83	31.371549279089734	34.0	30.5	35.0	20.0	37.0
84-85	31.077649358685044	34.0	30.0	35.0	20.0	36.5
86-87	30.789124936240537	34.0	30.0	35.0	20.0	36.0
88-89	30.52131365054625	34.0	29.5	35.0	19.0	36.0
90-91	30.235162034702768	34.0	29.0	35.0	18.0	35.0
92-93	30.024417794103684	34.0	29.0	35.0	18.0	35.0
94-95	29.80681604574821	34.0	29.0	35.0	18.0	35.0
96-97	29.603790249232194	34.0	29.0	35.0	17.0	35.0
98-99	29.43600189736415	33.0	28.5	35.0	16.5	35.0
100	27.57685793363533	31.0	25.0	34.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.319334360019333
1101	2	0.29540466440045066
1101	3	0.3081545702847919
1101	4	0.16250422444381485
1101	5	0.19431673862404608
1101	6	0.20915464248624716
1101	7	0.2154790790433836
1101	8	0.16897858676317412
1101	9	0.24068258947264098
1101	10-11	0.1878846448939271
1101	12-13	0.2590733490066839
1101	14-15	0.2671884051321882
1101	16-17	0.3537625160222646
1101	18-19	0.3476255840254794
1101	20-21	0.2604055923907751
1101	22-23	0.24984566524945961
1101	24-25	0.23523063808513456
1101	26-27	0.2723008654916228
1101	28-29	0.3230203698591936
1101	30-31	0.3826727951483946
1101	32-33	0.383721118872117
1101	34-35	0.3600966571005202
1101	36-37	0.3375433549470017
1101	38-39	0.34759023807391287
1101	40-41	0.35022711580574395
1101	42-43	0.3862139573741885
1101	44-45	0.3051342513538273
1101	46-47	0.42500773618824894
1101	48-49	0.41467701345453634
1101	50-51	0.37200915500053355
1101	52-53	0.30107189728049377
1101	54-55	0.24191917902095383
1101	56-57	0.26559202649009706
1101	58-59	0.23512501022790389
1101	60-61	0.399029955055866
1101	62-63	0.3630287146652691
1101	64-65	0.2172026514576899
1101	66-67	0.16793303561117057
1101	68-69	0.1988381898298215
1101	70-71	0.17821954757006608
1101	72-73	0.09125867266707388
1101	74-75	0.04734587848822969
1101	76-77	0.10732939299818867
1101	78-79	0.21010529856287619
1101	80-81	0.21544826932426275
1101	82-83	0.18320130928522715
1101	84-85	0.21738779225747962
1101	86-87	0.02669188448079396
1101	88-89	-0.04232783126660067
1101	90-91	-0.04510201938658298
1101	92-93	-0.11832610076306693
1101	94-95	-0.01368241663780978
1101	96-97	0.16655190228146566
1101	98-99	-0.13620229669731998
1101	100	-0.1935173321525845
1102	1	0.32198727868096455
1102	2	0.21461469726987303
1102	3	0.27348195353869187
1102	4	0.20632355089688303
1102	5	0.18380274058163337
1102	6	0.17814908716390931
1102	7	0.1675074382163686
1102	8	0.16087244129370504
1102	9	0.23096731171020934
1102	10-11	0.20103273514600772
1102	12-13	0.22324030474455014
1102	14-15	0.2971318079383565
1102	16-17	0.24457351354251244
1102	18-19	0.22531353185568292
1102	20-21	0.27301121384965654
1102	22-23	0.3047801473554017
1102	24-25	0.2280985888907452
1102	26-27	0.1855615554180261
1102	28-29	0.2635614632626968
1102	30-31	0.27169707491923134
1102	32-33	0.3555405900449031
1102	34-35	0.37823131750999295
1102	36-37	0.5139839881933526
1102	38-39	0.40636770996403726
1102	40-41	0.41245535205901973
1102	42-43	0.3438218508958286
1102	44-45	0.3913061901635331
1102	46-47	0.4180894063495515
1102	48-49	0.4566078488445129
1102	50-51	0.4724695423043812
1102	52-53	0.5178427032257176
1102	54-55	0.542809978602925
1102	56-57	0.4816123980920324
1102	58-59	0.5016694568572646
1102	60-61	0.41262210100994423
1102	62-63	0.5078236011615544
1102	64-65	0.5216299240912363
1102	66-67	0.41291921543039223
1102	68-69	0.4409811611281853
1102	70-71	0.42919106614021985
1102	72-73	0.3486624395777369
1102	74-75	0.37974106532396945
1102	76-77	0.41980798884130976
1102	78-79	0.34586740070332667
1102	80-81	0.3055358799906571
1102	82-83	0.3138782440552994
1102	84-85	0.22679185818191172
1102	86-87	0.19748361549802596
1102	88-89	0.3256793616348439
1102	90-91	0.3036194758001045
1102	92-93	0.16834971491482875
1102	94-95	0.13872595147612898
1102	96-97	0.1790757276397521
1102	98-99	0.11784345719667044
1102	100	0.14340039192546428
1103	1	0.3020979946538347
1103	2	0.26069515619272465
1103	3	0.3320730114228141
1103	4	0.1319618413221022
1103	5	0.20444008295322647
1103	6	0.23270959593259732
1103	7	0.1852619094829251
1103	8	0.19126634482218208
1103	9	0.22386038656100737
1103	10-11	0.228592306060186
1103	12-13	0.30320169647798423
1103	14-15	0.37777236766550004
1103	16-17	0.38761558811562935
1103	18-19	0.40816525858814856
1103	20-21	0.34660597354703526
1103	22-23	0.3561444869097272
1103	24-25	0.2903232419007935
1103	26-27	0.2518853697367476
1103	28-29	0.37043315985216196
1103	30-31	0.40887178706095284
1103	32-33	0.4157789664208025
1103	34-35	0.5044951378832678
1103	36-37	0.4107448204592572
1103	38-39	0.41673387654980587
1103	40-41	0.4989211101704498
1103	42-43	0.4891987335008281
1103	44-45	0.46277378643269884
1103	46-47	0.548639680775139
1103	48-49	0.5211859232618536
1103	50-51	0.48613774022830114
1103	52-53	0.4976369222775574
1103	54-55	0.6205840785198973
1103	56-57	0.581639912508706
1103	58-59	0.6147245723830466
1103	60-61	0.5573224812976605
1103	62-63	0.5163668854881678
1103	64-65	0.6165028315931878
1103	66-67	0.5681192315635286
1103	68-69	0.5458661406673784
1103	70-71	0.48309621256466784
1103	72-73	0.5818164540168951
1103	74-75	0.3966808363354062
1103	76-77	0.43136619956511524
1103	78-79	0.5809593850838581
1103	80-81	0.5329639519277691
1103	82-83	0.48209811924600743
1103	84-85	0.4474944516764552
1103	86-87	0.4449579218542503
1103	88-89	0.4518536384600118
1103	90-91	0.4084857724245232
1103	92-93	0.4166230519847467
1103	94-95	0.4992247296691339
1103	96-97	0.420983939360287
1103	98-99	0.45285491496934327
1103	100	0.6118425845300877
1104	1	0.26158856849085055
1104	2	0.24567176066005203
1104	3	0.29935456188884046
1104	4	0.06992309854649648
1104	5	0.14262128083470316
1104	6	0.1482420023787796
1104	7	0.19732283035525455
1104	8	0.22772402146364357
1104	9	0.27610301682665295
1104	10-11	0.23526274614912523
1104	12-13	0.24161894952723628
1104	14-15	0.34425800758380376
1104	16-17	0.2858313838279827
1104	18-19	0.26413963675206276
1104	20-21	0.18121732363632503
1104	22-23	0.276884569997371
1104	24-25	0.2655741375184064
1104	26-27	0.28512830766450037
1104	28-29	0.3870867398688489
1104	30-31	0.250630689443355
1104	32-33	0.26881462880033524
1104	34-35	0.35917834972467233
1104	36-37	0.26079384820924645
1104	38-39	0.3992214143469468
1104	40-41	0.5222916806854627
1104	42-43	0.5755807450825117
1104	44-45	0.439104802548151
1104	46-47	0.4874644445548171
1104	48-49	0.5209894583560981
1104	50-51	0.5197753067154096
1104	52-53	0.515069000676661
1104	54-55	0.4560343505082969
1104	56-57	0.6387239269140892
1104	58-59	0.5899694751957938
1104	60-61	0.4988552142809226
1104	62-63	0.5390774216884822
1104	64-65	0.6087111943400245
1104	66-67	0.5678454028501605
1104	68-69	0.6718307699062294
1104	70-71	0.587926318906284
1104	72-73	0.5992584417783604
1104	74-75	0.5812891428348195
1104	76-77	0.5681609596830448
1104	78-79	0.7078948015224427
1104	80-81	0.7416318363449648
1104	82-83	0.5289204226725737
1104	84-85	0.6616767075003303
1104	86-87	0.6562926044337765
1104	88-89	0.6848509185759788
1104	90-91	0.4821966351619231
1104	92-93	0.525228955699717
1104	94-95	0.5657787205940465
1104	96-97	0.5628763114162645
1104	98-99	0.6001372251531478
1104	100	0.6555496675535579
1105	1	0.2344141109062008
1105	2	0.1795501566298583
1105	3	0.23817351236736783
1105	4	0.12368715117014517
1105	5	0.11548235772742999
1105	6	0.11089196564039838
1105	7	0.14829185361150365
1105	8	0.1743960914420981
1105	9	0.1247003176044501
1105	10-11	0.1730558084987308
1105	12-13	0.19954928369890013
1105	14-15	0.3053602793048924
1105	16-17	0.34320490438982887
1105	18-19	0.28654854227462323
1105	20-21	0.20992524926168699
1105	22-23	0.2933594119757501
1105	24-25	0.28984758367143826
1105	26-27	0.3410957891516162
1105	28-29	0.427015453435331
1105	30-31	0.47977389299881423
1105	32-33	0.49181774651382426
1105	34-35	0.4582108993059464
1105	36-37	0.48066614545301434
1105	38-39	0.5316535085432079
1105	40-41	0.577459927697852
1105	42-43	0.5514013293702931
1105	44-45	0.4959470535466082
1105	46-47	0.534617773688737
1105	48-49	0.6439070506020457
1105	50-51	0.6591040507289136
1105	52-53	0.614480681772001
1105	54-55	0.5081328458659016
1105	56-57	0.5800915538012958
1105	58-59	0.6626066072100656
1105	60-61	0.6382201723929626
1105	62-63	0.6770745451434124
1105	64-65	0.6085940082356771
1105	66-67	0.5751778354589234
1105	68-69	0.6239149058397828
1105	70-71	0.7605730044521763
1105	72-73	0.7017216218461257
1105	74-75	0.7618367155387418
1105	76-77	0.6544499815878204
1105	78-79	0.7938921688642822
1105	80-81	0.6970898058544321
1105	82-83	0.7026978036378999
1105	84-85	0.6056843227524276
1105	86-87	0.6660360901679461
1105	88-89	0.6234903519816477
1105	90-91	0.8379809781663319
1105	92-93	0.8319166414304711
1105	94-95	0.8615980504953562
1105	96-97	0.8141830772584271
1105	98-99	0.791166643428511
1105	100	0.9742519872753093
1106	1	0.2386325695250875
1106	2	0.23538731035562677
1106	3	0.2333841622589219
1106	4	0.15632143722494618
1106	5	0.15550086461649215
1106	6	0.10601233316338465
1106	7	0.12610959523292564
1106	8	0.19742098526066343
1106	9	0.1783670074562167
1106	10-11	0.14270887507988306
1106	12-13	0.19037002815575477
1106	14-15	0.2837692408962198
1106	16-17	0.24596700676125494
1106	18-19	0.23448002971551318
1106	20-21	0.28648061218385834
1106	22-23	0.2735991824153885
1106	24-25	0.2514381656857978
1106	26-27	0.25504732485869397
1106	28-29	0.3420491319475829
1106	30-31	0.3672236173784995
1106	32-33	0.34285721944016956
1106	34-35	0.3801141592567916
1106	36-37	0.34019171260267456
1106	38-39	0.266194247316065
1106	40-41	0.39310965881384163
1106	42-43	0.3445070249122395
1106	44-45	0.4581674198538721
1106	46-47	0.5143230246747166
1106	48-49	0.6326686395797054
1106	50-51	0.6224216069027904
1106	52-53	0.5932257608348479
1106	54-55	0.4781879978302612
1106	56-57	0.4797594920609214
1106	58-59	0.6201255141915283
1106	60-61	0.6205047487962503
1106	62-63	0.6965522686166921
1106	64-65	0.7623662068863766
1106	66-67	0.7328604763916005
1106	68-69	0.664081242633678
1106	70-71	0.6632001815178228
1106	72-73	0.6894644948702435
1106	74-75	0.6907296344713529
1106	76-77	0.6070943382123453
1106	78-79	0.6911577092401302
1106	80-81	0.6689050654082749
1106	82-83	0.7422741162548512
1106	84-85	0.7814579659210317
1106	86-87	0.6008226241839729
1106	88-89	0.5991269174171308
1106	90-91	0.638988772544149
1106	92-93	0.7490051406724341
1106	94-95	0.8691528711254186
1106	96-97	0.9341550917171588
1106	98-99	0.9457041495743859
1106	100	1.023264252324413
1107	1	0.20333595835865736
1107	2	0.18311153879493247
1107	3	0.2451082598998866
1107	4	0.18059340287283732
1107	5	0.13287913143474128
1107	6	0.1617456871798666
1107	7	0.19623106621982345
1107	8	0.15298713273499942
1107	9	0.20703397692636827
1107	10-11	0.20354301560117705
1107	12-13	0.20380262932408755
1107	14-15	0.2366122007351521
1107	16-17	0.25927528968605174
1107	18-19	0.22287940292027741
1107	20-21	0.2965112102233931
1107	22-23	0.31559003689942955
1107	24-25	0.31860376356782183
1107	26-27	0.32585713210260536
1107	28-29	0.3124734030957441
1107	30-31	0.23263406497602546
1107	32-33	0.2560740133095578
1107	34-35	0.3899903813539751
1107	36-37	0.5215900745129147
1107	38-39	0.4277037339844014
1107	40-41	0.45951155188060966
1107	42-43	0.44786114508002584
1107	44-45	0.47403484416728503
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2316	90-91	0.05657293764258142
2316	92-93	0.11606113784318595
2316	94-95	0.1818178951514895
2316	96-97	0.1529582735720787
2316	98-99	0.04719843356345521
2316	100	0.10755549762058791
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	3.0
11	15.0
12	48.0
13	147.0
14	450.0
15	1258.0
16	2895.0
17	6006.0
18	10946.0
19	16420.0
20	22223.0
21	29578.0
22	37505.0
23	42841.0
24	45306.0
25	49016.0
26	55327.0
27	65533.0
28	78238.0
29	94288.0
30	111872.0
31	136424.0
32	166247.0
33	209445.0
34	275555.0
35	372584.0
36	536410.0
37	821335.0
38	780625.0
39	117105.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.108158781567646	19.520197223750657	13.645951841531733	23.72569215314996
2	15.4775809744983	21.114170693338668	39.79276915921521	23.61547917294782
3	17.383214490001667	27.057999119352676	31.034251502399506	24.524534888246148
4	12.156277336638572	17.46730128053565	37.6244666066515	32.75195477617427
5	12.392020276066825	37.95738941968203	35.11634139864231	14.534248905608838
6	30.344585358212374	37.29168170920799	18.57040582430883	13.793327108270809
7	26.67169725978714	33.16789323067856	22.736722611822596	17.423686897711708
8	26.017357404016124	35.864788671721456	20.41149549861784	17.706358425644588
9	26.972291182810544	15.245931692426232	20.385068092920935	37.39670903184229
10-11	24.679195666412447	26.11826266843023	29.22589402761066	19.976647637546662
12-13	25.147013903234427	24.140226273892054	28.98714481940285	21.72561500347067
14-15	22.618504489351558	25.472591018163982	26.836590736202748	25.072313756281716
16-17	22.070617900201817	28.1379604225585	27.479702973992666	22.31171870324702
18-19	21.771431900171017	27.175926730462667	29.509289516639676	21.543351852726644
20-21	22.62781896364352	26.60551446759599	29.438943748984226	21.327722819776266
22-23	22.395175036248663	26.371012035241236	29.342790456786993	21.891022471723108
24-25	22.03684245436547	26.432220247024535	29.434643107233168	22.09629419137683
26-27	21.96279209905757	26.54716264637001	29.392880888775863	22.097164365796555
28-29	22.10427767565834	26.927389411180158	29.302212877818107	21.666120035343386
30-31	22.645562320773134	26.542109807999015	29.42375227750903	21.388575593718823
32-33	22.348814446199405	26.60190441503843	29.348982599443552	21.70029853931861
34-35	22.46987373065191	26.878121165949192	29.18267903965537	21.469326063743534
36-37	22.432523887177258	26.885785179088817	29.097742382568097	21.58394855116583
38-39	22.382782903962894	27.04104291803422	28.94661964476082	21.62955453324207
40-41	22.487618242904205	26.714965758940394	29.11077517607625	21.686640822079156
42-43	22.268711640824222	26.944510669462314	28.75471373902861	22.032063950684854
44-45	22.0343932400169	27.183172298598883	28.88579824910524	21.896636212278977
46-47	22.04835868710634	26.865428693676098	28.635627754328354	22.45058486488921
48-49	22.132195984594	26.992768323329763	28.638453005973503	22.23658268610274
50-51	22.215203839812638	27.28832753796627	28.49765017458311	21.998818447637976
52-53	22.287924678118205	27.34688187950281	28.15935162009308	22.205841822285905
54-55	22.10114571500532	27.213111704581806	28.508977080559855	22.17676549985302
56-57	22.25300546608589	27.301828686230223	28.104118509206582	22.34104733847731
58-59	22.224835783179582	27.329132571220587	28.31612850087816	22.12990314472167
60-61	22.52802932615062	27.38981153209359	28.31057403942464	21.77158510233115
62-63	22.39414043585906	27.410857358554857	28.30125564186961	21.89374656371647
64-65	22.255128490690268	27.10567346549146	28.284228896759522	22.35496914705875
66-67	22.14994038911022	27.17680108909053	28.195932506838332	22.47732601496092
68-69	21.984093830707824	27.275216335676745	28.28809360354328	22.452596230072157
70-71	22.072289775929477	27.074159223044315	28.291973983170692	22.561577017855512
72-73	21.92315125082108	27.54367969443888	27.865109765131958	22.668059289608088
74-75	22.071566444240258	27.438731567335182	27.827443382108534	22.662258606316023
76-77	21.930133630510312	27.307724174173092	27.947208660558235	22.81493353475836
78-79	21.89246414308741	27.20705890704189	28.078089067359222	22.82238788251147
80-81	21.97899676618197	27.417276834674343	27.740756546728324	22.862969852415365
82-83	22.121818964954834	27.484732757621444	27.784306451097642	22.609141826326084
84-85	22.072506097377374	27.583989338433025	27.545910564944077	22.797593999245525
86-87	21.826136951886497	27.560266274865647	27.70689787920929	22.90669889403856
88-89	21.67094003964218	27.291509809377008	27.85180782221267	23.185742328768146
90-91	22.11722392717452	27.2839210124589	27.747140864150698	22.851714196215873
92-93	21.904291109024243	27.353183389787723	27.664450726268008	23.078074774920026
94-95	21.710670326360816	27.21326206560516	27.645740953397663	23.43032665463636
96-97	21.639152264177433	27.1626991145947	27.697200763965483	23.50094785726238
98-99	21.57689650170842	27.105532601903708	27.593412114917726	23.724158781470152
100	20.806959546266413	27.652891259048033	27.756787006862403	23.783362187823144
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2554.0
1	1892.0
2	2640.0
3	3703.0
4	2450.5
5	1743.0
6	2519.0
7	4707.0
8	7177.0
9	8167.5
10	6940.0
11	5395.0
12	5253.0
13	5512.5
14	5916.0
15	6220.5
16	6476.5
17	6860.5
18	7369.0
19	7797.5
20	8385.5
21	9531.0
22	11126.0
23	13419.5
24	16334.5
25	19786.0
26	24132.5
27	29173.0
28	34913.5
29	41335.0
30	48393.0
31	55123.0
32	62294.5
33	70293.5
34	78043.5
35	85400.5
36	94561.0
37	104078.0
38	111204.0
39	116960.0
40	120565.0
41	123492.5
42	126878.0
43	130964.5
44	135559.0
45	140998.0
46	145991.0
47	149024.5
48	153152.0
49	157833.5
50	161335.0
51	160138.0
52	153322.0
53	146602.5
54	139396.0
55	129008.5
56	116877.5
57	105418.0
58	93557.0
59	78838.0
60	63458.5
61	50701.5
62	40874.5
63	31828.5
64	23738.5
65	17722.0
66	13135.5
67	10038.0
68	7695.0
69	5694.5
70	4329.0
71	3304.0
72	2543.0
73	1937.0
74	1504.5
75	1181.5
76	834.0
77	531.0
78	340.5
79	248.5
80	176.5
81	110.0
82	81.0
83	62.0
84	45.5
85	29.0
86	16.5
87	13.5
88	11.0
89	8.0
90	4.0
91	2.0
92	2.0
93	2.0
94	2.0
95	1.0
96	2.5
97	3.0
98	0.5
99	0.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0038182250735987362
2	0.06571752770905516
3	9.056046648920079E-4
4	8.321772596304938E-4
5	0.0015419755104917972
6	8.077014578766556E-4
7	0.0
8	8.321772596304938E-4
9	2.4475801753838052E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	7.954635569997366E-4
18-19	0.0010035078719073602
20-21	0.0030349994174759184
22-23	0.0
24-25	2.4475801753838052E-5
26-27	8.566530613843318E-5
28-29	9.300804666458459E-4
30-31	0.0015664513122456353
32-33	0.0026678623911683473
34-35	0.003805987172721817
36-37	0.0013584069973380118
38-39	0.0013339311955841736
40-41	0.005433627989352047
42-43	0.006608466473536274
44-45	0.005237821575321343
46-47	0.006853224491074654
48-49	0.009239615162073864
50-51	0.00892142973927397
52-53	0.003965079884121764
54-55	0.002374152770122291
56-57	0.0027412897964298618
58-59	0.003414374344660408
60-61	0.007440643733166767
62-63	0.014342819827749096
64-65	0.0038671766771064117
66-67	0.010389977844504251
68-69	0.012813082218134219
70-71	0.005764051313028861
72-73	0.008113728281397314
74-75	0.002986047813968242
76-77	0.0027290518955529427
78-79	0.00815044198402807
80-81	0.006106712537582593
82-83	0.017047395921548202
84-85	0.017500198253994206
86-87	0.023043967351238526
88-89	0.021477516038992887
90-91	0.024439088051207297
92-93	0.03420493295098868
94-95	0.03764378309740292
96-97	0.02272578192843863
98-99	0.018246710207486267
100	0.019678544610085794
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4085668.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.71428286891796
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.87959689609586	51.17801860147348
2	14.494865805277465	19.050394233500196
3	3.9474605542893224	7.782136184353927
4	1.434032224979655	3.769455971018274
5	0.7050547150271641	2.31660824906797
6	0.3935890976019184	1.5518655176360776
7	0.26116439264891794	1.2013561541674107
8	0.16912513436121052	0.8891149535725076
9	0.12069766400116205	0.7138404390410982
>10	0.5495950200350972	6.194969795579841
>50	0.029414558929780423	1.3304208807492774
>100	0.014204900981578995	1.7574026507320564
>500	6.661309885223649E-4	0.2959727693053188
>1k	3.552698548982E-4	0.500311289139194
>5k	4.440873186227499E-5	0.15662451521290566
>10k+	1.3322619558682496E-4	1.3115077954505667
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17456	0.427249595414997	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16308	0.3991513750015909	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11177	0.2735660362026479	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5367	0.1313616280128488	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	2.4475801753838052E-5	0.0	0.0	0.0
16-17	0.0	4.8951603507676104E-5	0.0	0.0	2.4475801753838052E-5
18-19	0.0	4.8951603507676104E-5	0.0	0.0	2.4475801753838052E-5
20-21	0.0	4.8951603507676104E-5	0.0	4.8951603507676104E-5	4.8951603507676104E-5
22-23	0.0	4.8951603507676104E-5	0.0	1.3461690964610928E-4	8.566530613843318E-5
24-25	0.0	4.8951603507676104E-5	0.0	2.6923381929221856E-4	1.2237900876919025E-4
26-27	0.0	4.8951603507676104E-5	0.0	5.384676385844371E-4	1.2237900876919025E-4
28-29	0.0	4.8951603507676104E-5	0.0	0.001945826239430125	1.2237900876919025E-4
30-31	0.0	4.8951603507676104E-5	0.0	0.006192377843721027	1.2237900876919025E-4
32-33	0.0	6.118950438459513E-5	0.0	0.015554372014564081	1.468548105230283E-4
34-35	0.0	7.342740526151415E-5	0.0	0.030961889218605133	1.468548105230283E-4
36-37	0.0	7.342740526151415E-5	0.0	0.052671925374259486	1.468548105230283E-4
38-39	0.0	7.342740526151415E-5	0.0	0.08933667640150889	2.447580175383805E-4
40-41	0.0	8.566530613843318E-5	0.0	0.1344333411329555	2.937096210460566E-4
42-43	0.0	9.790320701535221E-5	0.0	0.17614010732149554	2.937096210460566E-4
44-45	0.0	9.790320701535221E-5	0.0	0.2262910251151097	2.937096210460566E-4
46-47	0.0	9.790320701535221E-5	0.0	0.28909593241545817	2.937096210460566E-4
48-49	0.0	9.790320701535221E-5	0.0	0.3565634799499127	3.671370263075708E-4
50-51	0.0	9.790320701535221E-5	0.0	0.43189999774822624	3.671370263075708E-4
52-53	0.0	9.790320701535221E-5	0.0	0.5102837528648926	4.0385072893832786E-4
54-55	0.0	9.790320701535221E-5	0.0	0.6024963359724775	4.6504023332292295E-4
56-57	0.0	9.790320701535221E-5	0.0	0.706702061939443	4.77278134199842E-4
58-59	0.0	9.790320701535221E-5	0.0	0.8320304048199707	4.89516035076761E-4
60-61	0.0	9.790320701535221E-5	0.0	0.9598919931820207	4.89516035076761E-4
62-63	2.4475801753838052E-5	9.790320701535221E-5	0.0	1.0931627337316687	4.89516035076761E-4
64-65	2.4475801753838052E-5	9.790320701535221E-5	0.0	1.232197525594346	4.89516035076761E-4
66-67	2.4475801753838052E-5	9.790320701535221E-5	0.0	1.3947902766450921	4.89516035076761E-4
68-69	2.4475801753838052E-5	9.790320701535221E-5	0.0	1.5854445344066135	4.89516035076761E-4
70-71	2.4475801753838052E-5	9.790320701535221E-5	0.0	1.7988857636009583	4.89516035076761E-4
72-73	2.4475801753838052E-5	9.790320701535221E-5	0.0	2.004029696000752	5.629434403382752E-4
74-75	2.4475801753838052E-5	9.790320701535221E-5	0.0	2.214692921696036	5.629434403382752E-4
76-77	2.4475801753838052E-5	9.790320701535221E-5	0.0	2.453907170137172	5.629434403382752E-4
78-79	2.4475801753838052E-5	9.790320701535221E-5	0.0	2.7310221975941267	5.629434403382752E-4
80-81	2.4475801753838052E-5	9.790320701535221E-5	0.0	3.017364602312278	5.996571429690322E-4
82-83	2.4475801753838052E-5	9.790320701535221E-5	0.0	3.317511359219594	6.608466473536274E-4
84-85	2.4475801753838052E-5	9.790320701535221E-5	0.0	3.6259064613179532	6.608466473536274E-4
86-87	2.4475801753838052E-5	9.790320701535221E-5	0.0	3.949855935430877	6.608466473536274E-4
88	2.4475801753838052E-5	9.790320701535221E-5	0.0	4.211893869007467	6.608466473536274E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8285	0.0	48.807426	1
TACCTGG	4165	0.0	42.107864	2
GTACCTG	4700	0.0	37.715816	1
GTATCAA	13685	0.0	35.56113	1
ACCTGGG	5015	0.0	34.483997	3
TATAACG	445	0.0	28.528118	2
ATCAACG	17280	0.0	27.766602	3
TCAACGC	17270	0.0	27.728256	4
CAACGCA	17415	0.0	27.578342	5
TAACGCA	450	0.0	27.151985	4
TATCAAC	17960	0.0	26.860271	2
GTACATG	14535	0.0	26.494081	1
AACGCAG	18175	0.0	26.450346	6
TACATGG	14975	0.0	24.930042	2
CCTGGGG	4975	0.0	24.370665	4
ACATGGG	14925	0.0	24.024311	3
CATGGGG	9470	0.0	23.472097	4
ACGCAGA	20405	0.0	23.283306	7
ATGGGGG	6245	0.0	22.800844	5
CGCAGAG	21030	0.0	22.54665	8
>>END_MODULE