FastQCFastQC Report
Mon 6 Jun 2016
ERR1211532_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211532_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6615548
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218390.3301162655006055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195310.29522875504795676No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122060.18450474548744866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113350.048.8635751
GTATCAA190150.036.3109051
TACCTGG59900.031.1653922
TCAACGC225850.029.8363464
CAACGCA228350.029.4667535
ATCAACG228300.029.4550763
AACGCAG236650.028.6076456
GTACCTG67300.028.1326391
TATCAAC242200.027.879772
GTACATG213800.026.6549031
TACATGG220150.025.2468782
ACGCAGA268000.025.1039877
ACATGGG218900.024.6446443
CGCAGAG272400.024.5440048
ACCTGGG74800.024.1243723
CATGGGG125000.022.3678134
TAACGCA6800.021.4224594
GCAGAGT307250.021.1038199
GAGTACT184150.020.99964112-13
TATAACG6900.020.4447152
Produced by FastQC (version 0.11.5)