FastQCFastQC Report
Mon 6 Jun 2016
ERR1211532_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211532_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6615548
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233310.3526691968677425No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208750.3155445323652704No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144300.21812251985776537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117550.048.4023441
GTATCAA199450.035.9062081
TACCTGG58000.031.2934272
GTACCTG63750.029.5779321
TCAACGC242150.029.1282374
ATCAACG242250.029.0192243
CAACGCA245150.028.7334465
TATCAAC254800.027.921152
AACGCAG253400.027.8906886
GTACATG221550.026.3817181
TACATGG226100.025.4550912
ACGCAGA286150.024.6325267
ACATGGG225050.024.5762393
ACCTGGG72950.024.4136033
CGCAGAG289300.024.266868
CATGGGG124550.023.3541954
ATGGGGG78850.021.8120945
GCAGAGT326350.021.1659899
GAGTACT200000.020.8998812-13
AGAGTAC299850.019.37766810-11
Produced by FastQC (version 0.11.5)