##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1211532_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6615548
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15225118161035	33.0	31.0	34.0	30.0	34.0
2	32.36500211320362	34.0	31.0	34.0	31.0	34.0
3	32.37906247524771	34.0	31.0	34.0	30.0	34.0
4	36.00389808977276	37.0	35.0	37.0	35.0	37.0
5	35.92936987230687	37.0	35.0	37.0	35.0	37.0
6	35.950435398549	37.0	35.0	37.0	35.0	37.0
7	35.879854095231416	37.0	35.0	37.0	35.0	37.0
8	35.87671694015371	37.0	35.0	37.0	35.0	37.0
9	37.53735215888389	39.0	37.0	39.0	35.0	39.0
10-11	37.50145959185845	39.0	37.0	39.0	35.0	39.0
12-13	37.41518004253011	39.0	37.0	39.0	34.0	39.0
14-15	38.66655763059991	40.0	38.0	41.0	34.0	41.0
16-17	38.59611924817113	40.0	38.0	41.0	34.0	41.0
18-19	38.58443896106566	40.0	38.0	41.0	34.0	41.0
20-21	38.52615482496688	40.0	38.0	41.0	34.0	41.0
22-23	38.41817881149075	40.0	38.0	41.0	34.0	41.0
24-25	38.31484028231675	40.0	38.0	41.0	34.0	41.0
26-27	38.14230922366522	40.0	38.0	41.0	33.0	41.0
28-29	38.18203699829553	40.0	38.0	41.0	33.5	41.0
30-31	38.1040987836533	40.0	37.5	41.0	33.0	41.0
32-33	37.921291705539744	40.0	37.0	41.0	33.0	41.0
34-35	37.81135742647472	40.0	37.0	41.0	33.0	41.0
36-37	37.757140980611126	40.0	37.0	41.0	33.0	41.0
38-39	37.664673281790115	40.0	37.0	41.0	33.0	41.0
40-41	37.50902888165878	40.0	37.0	41.0	32.0	41.0
42-43	37.362003873299685	40.0	36.5	41.0	31.0	41.0
44-45	37.18355947232187	40.0	36.0	41.0	31.0	41.0
46-47	36.93285461763712	39.5	36.0	41.0	30.5	41.0
48-49	36.71978496717128	39.0	36.0	41.0	30.0	41.0
50-51	36.52783374861765	39.0	35.0	41.0	29.5	41.0
52-53	36.33415281696996	39.0	35.0	40.5	29.0	41.0
54-55	36.14554803320904	39.0	35.0	40.0	28.0	41.0
56-57	35.95059290628683	38.5	35.0	40.0	28.0	41.0
58-59	35.70102529677058	38.0	34.0	40.0	28.0	41.0
60-61	35.3972930133679	38.0	34.0	40.0	27.0	41.0
62-63	35.0784504171083	37.5	34.0	40.0	26.0	41.0
64-65	34.76598771560572	37.0	33.5	40.0	26.0	41.0
66-67	34.42831992149404	36.5	33.0	39.0	26.0	41.0
68-69	34.028880600669815	36.0	33.0	39.0	25.5	40.5
70-71	33.64326167688603	35.5	32.0	39.0	25.0	40.0
72-73	33.23634497096839	35.0	32.0	38.0	24.0	40.0
74-75	32.82754459645671	35.0	31.5	37.0	23.5	39.5
76-77	31.257765267518277	33.5	29.5	35.5	21.5	38.5
78-79	32.09165574794409	34.5	31.0	36.0	23.0	39.0
80-81	32.05848041613484	35.0	31.0	36.0	23.0	38.0
82-83	31.768962677014812	34.0	31.0	35.5	22.5	37.0
84-85	31.46623242700378	34.0	31.0	35.0	21.5	37.0
86-87	31.16543285605365	34.0	30.0	35.0	20.0	36.0
88-89	30.88571385167185	34.0	30.0	35.0	20.0	36.0
90-91	30.603306559033356	34.0	30.0	35.0	19.5	36.0
92-93	30.393166295520793	34.0	29.0	35.0	19.0	35.0
94-95	30.169787068282176	34.0	29.0	35.0	18.5	35.0
96-97	29.960576584131807	34.0	29.0	35.0	18.0	35.0
98-99	29.80514063234066	34.0	29.0	35.0	18.0	35.0
100	27.9348581553637	31.0	25.0	34.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3202539571631746
1101	2	0.2494538612934818
1101	3	0.30876675250446795
1101	4	0.18041065261750333
1101	5	0.2194747146043099
1101	6	0.17660414105930045
1101	7	0.18485574942427263
1101	8	0.22825562857040183
1101	9	0.19579293526107477
1101	10-11	0.21128441474436954
1101	12-13	0.246377003755903
1101	14-15	0.25930648660223454
1101	16-17	0.3261353908512774
1101	18-19	0.37413643276573083
1101	20-21	0.31795873144546505
1101	22-23	0.3198589380780419
1101	24-25	0.3152273925813631
1101	26-27	0.28816524262713017
1101	28-29	0.3052954255454523
1101	30-31	0.33541497990131575
1101	32-33	0.34426436307882113
1101	34-35	0.29159923174746183
1101	36-37	0.3292060336986964
1101	38-39	0.32797321384665423
1101	40-41	0.40453413072766864
1101	42-43	0.38044533369305356
1101	44-45	0.3761591466413421
1101	46-47	0.3783815968274027
1101	48-49	0.3884493118356076
1101	50-51	0.31897036471687557
1101	52-53	0.4013278460852021
1101	54-55	0.3326407279490411
1101	56-57	0.34183744217310874
1101	58-59	0.3190667375987104
1101	60-61	0.2340883353445875
1101	62-63	0.23028529596752634
1101	64-65	0.23779814669023125
1101	66-67	0.16260385174373226
1101	68-69	0.14624002706830908
1101	70-71	0.08029324799625925
1101	72-73	0.054458345325258506
1101	74-75	0.05668954768955814
1101	76-77	0.1893468933152569
1101	78-79	0.25991444889475446
1101	80-81	0.13923787983992497
1101	82-83	0.08436115360008145
1101	84-85	0.19012518281362745
1101	86-87	0.08793100081680905
1101	88-89	0.10145651405510492
1101	90-91	0.05020781774604899
1101	92-93	-0.04800513234092918
1101	94-95	0.010306477449201878
1101	96-97	-0.09848641194657048
1101	98-99	-0.2113299012939045
1101	100	-0.12138138335066984
1102	1	0.31747916462942527
1102	2	0.23996643962463793
1102	3	0.32054309603514497
1102	4	0.18016070544531004
1102	5	0.19857731784767196
1102	6	0.14502630119568494
1102	7	0.14822264124052253
1102	8	0.17526681035980118
1102	9	0.2523156303970211
1102	10-11	0.2146704499359302
1102	12-13	0.26563501195457917
1102	14-15	0.33632651428340665
1102	16-17	0.28314586720745183
1102	18-19	0.29508040511331757
1102	20-21	0.28245374980142657
1102	22-23	0.2644482210104897
1102	24-25	0.26207258331339744
1102	26-27	0.28987026090978674
1102	28-29	0.37148395462759254
1102	30-31	0.4299930261891376
1102	32-33	0.46799668594753996
1102	34-35	0.40296975817381764
1102	36-37	0.3623214045750771
1102	38-39	0.38591398203298155
1102	40-41	0.42771991814642973
1102	42-43	0.41388612636768585
1102	44-45	0.40004356792421447
1102	46-47	0.44665799753288127
1102	48-49	0.45743088901500784
1102	50-51	0.4581050534853688
1102	52-53	0.429776441552427
1102	54-55	0.459313329769131
1102	56-57	0.5111356515661569
1102	58-59	0.463902058073451
1102	60-61	0.47205312048652814
1102	62-63	0.4613334801374407
1102	64-65	0.42848103119109027
1102	66-67	0.4948012971193094
1102	68-69	0.4591924343715661
1102	70-71	0.4379102021487995
1102	72-73	0.41164391218990204
1102	74-75	0.3788965518269336
1102	76-77	0.43642801285902166
1102	78-79	0.5033520961588067
1102	80-81	0.4270422214201872
1102	82-83	0.4467264400245661
1102	84-85	0.35868253681860907
1102	86-87	0.32540893256734904
1102	88-89	0.3491082394785394
1102	90-91	0.31729772545789103
1102	92-93	0.3494228499922194
1102	94-95	0.30372352832966953
1102	96-97	0.33660369209668417
1102	98-99	0.30170883835757323
1102	100	0.3114869601812842
1103	1	0.29814324594258323
1103	2	0.22956909104117074
1103	3	0.3405711032396823
1103	4	0.16938179798536623
1103	5	0.18327803191609604
1103	6	0.19875599365857965
1103	7	0.19909054290178574
1103	8	0.21788173550911694
1103	9	0.23770172155933977
1103	10-11	0.2728919702570849
1103	12-13	0.2638268786073965
1103	14-15	0.3720986737855938
1103	16-17	0.36720861533956395
1103	18-19	0.3367004609115938
1103	20-21	0.30699112116513305
1103	22-23	0.37509126543789506
1103	24-25	0.3241642407592309
1103	26-27	0.3528162717811014
1103	28-29	0.38456695522074114
1103	30-31	0.4924228798579122
1103	32-33	0.4830101680613481
1103	34-35	0.4719774115603812
1103	36-37	0.4616816525340468
1103	38-39	0.5055444167594985
1103	40-41	0.5563718866386083
1103	42-43	0.5488900515583381
1103	44-45	0.45314366749323653
1103	46-47	0.585541260993736
1103	48-49	0.599843811132672
1103	50-51	0.5747906254992046
1103	52-53	0.506426277760319
1103	54-55	0.5127380132781383
1103	56-57	0.5066100140084089
1103	58-59	0.5985317041174696
1103	60-61	0.58243518614767
1103	62-63	0.6820729126984588
1103	64-65	0.6254308929402654
1103	66-67	0.6686494458515426
1103	68-69	0.6871222644872077
1103	70-71	0.6495822395481738
1103	72-73	0.6591900433508613
1103	74-75	0.6100069653749429
1103	76-77	0.6387904574922594
1103	78-79	0.701662130778395
1103	80-81	0.653748308199873
1103	82-83	0.6315789603653634
1103	84-85	0.6130522194698322
1103	86-87	0.5788941054896206
1103	88-89	0.6352560628095425
1103	90-91	0.6176371430878014
1103	92-93	0.5703970109925258
1103	94-95	0.5963575097730498
1103	96-97	0.6212989341897419
1103	98-99	0.6546961553361861
1103	100	0.6065179392656184
1104	1	0.2718918931668881
1104	2	0.24162423741869787
1104	3	0.2985358906385329
1104	4	0.09320025574078983
1104	5	0.1573403256363406
1104	6	0.20013092080290562
1104	7	0.210180609228118
1104	8	0.22338770074178171
1104	9	0.2727365143497593
1104	10-11	0.256307990896957
1104	12-13	0.2802892238853758
1104	14-15	0.3352713582697646
1104	16-17	0.3233935831933792
1104	18-19	0.3500930306667911
1104	20-21	0.286139035948473
1104	22-23	0.3761851855746201
1104	24-25	0.3341335701282304
1104	26-27	0.3943088461076414
1104	28-29	0.45037015281285875
1104	30-31	0.3827546782492277
1104	32-33	0.39211489817961365
1104	34-35	0.46295250262183885
1104	36-37	0.3682781238672064
1104	38-39	0.4236705748736469
1104	40-41	0.495349135414358
1104	42-43	0.5299594105252936
1104	44-45	0.49172907604705074
1104	46-47	0.4594844739258761
1104	48-49	0.46838106586273653
1104	50-51	0.46955488448914906
1104	52-53	0.43618812414054986
1104	54-55	0.45214642879065536
1104	56-57	0.4791058654480693
1104	58-59	0.516340110935765
1104	60-61	0.47876707184284584
1104	62-63	0.527699461763163
1104	64-65	0.6413898487023104
1104	66-67	0.5766060887487043
1104	68-69	0.5424418464876553
1104	70-71	0.4057446563183831
1104	72-73	0.5694801474630466
1104	74-75	0.5654013162154854
1104	76-77	0.4464975430206657
1104	78-79	0.5129340542073422
1104	80-81	0.6322893002869705
1104	82-83	0.4053403089617582
1104	84-85	0.4339831801347849
1104	86-87	0.48881653164264094
1104	88-89	0.39779141652901373
1104	90-91	0.40791133323428497
1104	92-93	0.5021378501279123
1104	94-95	0.5263535557838424
1104	96-97	0.44609369600212645
1104	98-99	0.4819899356131536
1104	100	0.6360847769089553
1105	1	0.2390222670970772
1105	2	0.1291416584916334
1105	3	0.20130958000479637
1105	4	0.12490893943803627
1105	5	0.1185280176899326
1105	6	0.14201163523672022
1105	7	0.12614393961779768
1105	8	0.13717146380601974
1105	9	0.12223681426527122
1105	10-11	0.18236872648839153
1105	12-13	0.21767226016693542
1105	14-15	0.3483970369275795
1105	16-17	0.31406430911618344
1105	18-19	0.28586589879883206
1105	20-21	0.26909786153765225
1105	22-23	0.32283879670385573
1105	24-25	0.32941996464950307
1105	26-27	0.3693968875520639
1105	28-29	0.36690724828396526
1105	30-31	0.37254529497112543
1105	32-33	0.42138765635003494
1105	34-35	0.48335932768652157
1105	36-37	0.47721011164326654
1105	38-39	0.4226124440258303
1105	40-41	0.42289808054498934
1105	42-43	0.45287012679575156
1105	44-45	0.4206493224972334
1105	46-47	0.4953045918852368
1105	48-49	0.5167203716068016
1105	50-51	0.5833285597217213
1105	52-53	0.521768899830235
1105	54-55	0.47511423667542374
1105	56-57	0.5213546220159841
1105	58-59	0.5402363119763862
1105	60-61	0.6533928952415167
1105	62-63	0.5519050686069988
1105	64-65	0.6175425328536193
1105	66-67	0.5765938790129681
1105	68-69	0.6389788990396923
1105	70-71	0.6558920518781477
1105	72-73	0.5717881020096769
1105	74-75	0.6091281167926113
1105	76-77	0.5857488390656123
1105	78-79	0.7222727205312509
1105	80-81	0.6669539055062614
1105	82-83	0.6546189540747598
1105	84-85	0.5781216449018522
1105	86-87	0.6035083142537019
1105	88-89	0.6528088964306811
1105	90-91	0.6114689116840566
1105	92-93	0.586036114983866
1105	94-95	0.6749638085135352
1105	96-97	0.7925874317417083
1105	98-99	0.7633141976308302
1105	100	0.851166468180832
1106	1	0.26054341277604465
1106	2	0.20921697859925814
1106	3	0.25443111829015663
1106	4	0.18120207445752357
1106	5	0.194755073289393
1106	6	0.1912867420337463
1106	7	0.19738072156062003
1106	8	0.1883243576907958
1106	9	0.1863080193378579
1106	10-11	0.19215086357786504
1106	12-13	0.22945274791649695
1106	14-15	0.28484999993799676
1106	16-17	0.33106785459227694
1106	18-19	0.2918956553246588
1106	20-21	0.3209167557769419
1106	22-23	0.3443335714635154
1106	24-25	0.38676825769231016
1106	26-27	0.4026790664041613
1106	28-29	0.4227760297641936
1106	30-31	0.39478974649568954
1106	32-33	0.5013712971204853
1106	34-35	0.43774889995795974
1106	36-37	0.4512517257287172
1106	38-39	0.46913793604047527
1106	40-41	0.4570192344630968
1106	42-43	0.5349387047918768
1106	44-45	0.5364969222050107
1106	46-47	0.5758425605288906
1106	48-49	0.5961541631195075
1106	50-51	0.5625270821943502
1106	52-53	0.6057810079655539
1106	54-55	0.5348407595652986
1106	56-57	0.5572206407384854
1106	58-59	0.5870858516616408
1106	60-61	0.663033389030609
1106	62-63	0.69491742694008
1106	64-65	0.7177955651786405
1106	66-67	0.7007803111135686
1106	68-69	0.6400815266074815
1106	70-71	0.6509764312610997
1106	72-73	0.635483963202077
1106	74-75	0.6642039943650317
1106	76-77	0.578413792898079
1106	78-79	0.6602216922827111
1106	80-81	0.718027752772997
1106	82-83	0.6954091349087754
1106	84-85	0.7087175048470407
1106	86-87	0.6762047563433136
1106	88-89	0.6818295025971075
1106	90-91	0.7604297878599766
1106	92-93	0.7315931489234586
1106	94-95	0.7638693804024932
1106	96-97	0.7800826365996265
1106	98-99	0.8838015201255338
1106	100	0.9550018826530717
1107	1	0.18533368489251956
1107	2	0.16013317470261512
1107	3	0.2396616631463715
1107	4	0.1476007381823905
1107	5	0.12508303409863686
1107	6	0.14908999047870708
1107	7	0.14314315876691097
1107	8	0.17254085420675835
1107	9	0.2334883790850668
1107	10-11	0.19933552383739794
1107	12-13	0.21252275277795007
1107	14-15	0.22107090915356054
1107	16-17	0.24966725875317763
1107	18-19	0.22217655980342954
1107	20-21	0.26516248331144254
1107	22-23	0.2851351495645531
1107	24-25	0.3072617570628111
1107	26-27	0.35820989920771495
1107	28-29	0.3350655228023456
1107	30-31	0.27938331691813545
1107	32-33	0.25233454955431966
1107	34-35	0.30857210527314294
1107	36-37	0.3893215436113664
1107	38-39	0.37532288300082683
1107	40-41	0.40133570230897675
1107	42-43	0.36329310440296325
1107	44-45	0.39501910412290897
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2316	74-75	-0.3809476631544868
2316	76-77	-0.41962473188462823
2316	78-79	-0.238415252689677
2316	80-81	-0.21014545214439195
2316	82-83	-0.1168886728611902
2316	84-85	-0.21637438183751456
2316	86-87	-0.1563093084293854
2316	88-89	-0.14696863855127873
2316	90-91	-0.04947896611149716
2316	92-93	0.08949297167528769
2316	94-95	0.1784625163663165
2316	96-97	0.15863139645564317
2316	98-99	0.05321173692827941
2316	100	-0.3063920662320676
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	5.0
11	28.0
12	74.0
13	209.0
14	597.0
15	1609.0
16	3823.0
17	7909.0
18	14136.0
19	21765.0
20	30200.0
21	41326.0
22	53313.0
23	61158.0
24	65273.0
25	71859.0
26	82158.0
27	98410.0
28	119007.0
29	142775.0
30	171828.0
31	207742.0
32	254717.0
33	321816.0
34	420834.0
35	575251.0
36	846587.0
37	1348363.0
38	1415447.0
39	237290.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.218975542321935	20.021858402598216	13.769871532756236	23.98929452232361
2	14.956566803441982	21.39284224309097	40.16119277998173	23.489398173485316
3	17.22395107371276	26.89951164711134	30.982330444109845	24.894206835066058
4	11.812116855957113	17.776674307818237	38.56660763858184	31.84460119764281
5	12.285697787473637	38.351298420176846	35.81286702107076	13.550136771278757
6	30.26726308381039	38.32979316466289	18.231747724738735	13.171196026787982
7	26.344590047566737	33.1238016865723	23.054325960600693	17.477282305260275
8	25.30241191871187	36.592174717480816	20.593229818546803	17.51218354526051
9	26.360616892299305	15.168239300544611	20.51500805602318	37.956135751132905
10-11	24.208576523063545	26.56782930151818	29.508001453545496	19.715592721872778
12-13	24.796509676900538	24.48562084350382	29.219710899233142	21.498158580362503
14-15	22.185954965484342	25.90013707103327	26.8592337324134	25.05467423106899
16-17	21.732080778850378	28.731558066563135	27.55040666653163	21.98595448805486
18-19	21.538479328918836	27.901341612105195	29.453165411881294	21.107013647094675
20-21	22.33326324353323	27.17260366576205	29.423400792372494	21.07073229833222
22-23	22.09527631688836	27.070110221414023	29.17053350459656	21.664079957101055
24-25	21.792619718369473	27.14835220730803	29.349697009181554	21.709331065140947
26-27	21.80282926240116	27.255074623154375	29.265802242462275	21.676293871982192
28-29	21.792131821418366	27.408022573668138	29.33816522133527	21.461680383578223
30-31	22.17166129930814	27.242796655678	29.306608929110883	21.278933115902973
32-33	21.887167738266594	27.324732417462315	29.231285396885	21.556814447386092
34-35	22.02496454599716	27.43564256681312	29.22519389445149	21.31419899273823
36-37	22.081003310730715	27.402600092087347	29.048691715675616	21.467704881506315
38-39	21.96562906695083	27.536819332397087	29.06800140187103	21.42955019878105
40-41	22.093833231824426	27.378441382498416	29.129927320226816	21.397798065450342
42-43	21.912609002166043	27.47523134131668	28.91528554487799	21.696874111639293
44-45	21.899016306013134	27.614488039379808	28.8898375505864	21.596658104020655
46-47	21.866092742282323	27.499738852714593	28.659708987442407	21.974459417560677
48-49	21.874167367416547	27.609595706686573	28.675720670074355	21.84051625582252
50-51	21.993724095264046	27.79522092462074	28.48956894443787	21.721486035677344
52-53	21.978918007309307	27.844804136008733	28.3626139627179	21.813663893964062
54-55	21.89962510899774	27.72139871733932	28.539672531753425	21.83930364190952
56-57	21.989233634100465	27.81713533704678	28.242014071070653	21.951616957782104
58-59	21.896637431353728	27.789815027773685	28.51220371063064	21.801343830241947
60-61	22.135455975050746	27.921214235314974	28.309821841254628	21.633507948379656
62-63	21.964343014890275	27.906037483261336	28.415406786778092	21.714212715070293
64-65	21.863799797876258	27.64617748266526	28.44013786620001	22.04988485325848
66-67	21.861566411545567	27.730641705591186	28.29936358932747	22.108428293535777
68-69	21.795982769779297	27.78405592308112	28.36033674913151	22.059624558008075
70-71	21.808593620089674	27.618561360757294	28.387048497839807	22.185796521313232
72-73	21.73375694604592	27.910124626521416	28.092180436158433	22.263937991274233
74-75	21.785409151319538	27.911613773537674	28.036709157728872	22.266267917413916
76-77	21.642569424530738	27.738476080400293	28.208519308656278	22.410435186412688
78-79	21.611731745187345	27.673892809518474	28.225698559140284	22.488676886153897
80-81	21.59009587939024	27.901203382641093	28.013996885348348	22.49470385262032
82-83	21.73500604361837	27.845894156928463	28.037483284613984	22.38161651483918
84-85	21.672451337030665	27.904459127399473	27.915042224288783	22.508047311281082
86-87	21.482066829135498	27.8855724909629	28.07106576343934	22.561294916462256
88-89	21.489454059214268	27.67029220077248	28.097770884670037	22.742482855343216
90-91	21.67543732522848	27.68877592330053	28.044826180841053	22.59096057062994
92-93	21.565756149733755	27.689658642531583	27.981057025879576	22.76352818185509
94-95	21.368952601936474	27.61363340795579	28.036250363686182	22.98116362642155
96-97	21.363752441255272	27.558579920842625	28.068678859247164	23.008988778654935
98-99	21.25358070559271	27.49067962007593	27.98421110480992	23.271528569521436
100	20.484714137614112	27.983312995927427	28.17116501057036	23.360807855888098
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2447.0
1	1824.0
2	2106.0
3	2921.0
4	2210.0
5	1801.0
6	2688.5
7	5177.0
8	8123.0
9	9474.0
10	8202.0
11	6568.5
12	6623.0
13	7040.0
14	7543.5
15	8017.5
16	8550.0
17	9166.5
18	9858.0
19	10767.5
20	12028.0
21	13841.0
22	16379.0
23	20326.0
24	25409.5
25	31426.0
26	39429.0
27	49297.0
28	60240.0
29	72718.5
30	86411.5
31	99346.0
32	113741.5
33	129814.0
34	145840.5
35	160384.0
36	175778.5
37	192077.0
38	203661.5
39	212285.0
40	217393.0
41	221104.5
42	226395.0
43	231841.0
44	237022.5
45	243506.5
46	247908.0
47	249908.5
48	253408.5
49	254595.5
50	253558.0
51	244906.5
52	230070.5
53	217201.5
54	203252.5
55	184802.5
56	164721.5
57	146255.5
58	126793.5
59	106057.5
60	86024.0
61	68988.0
62	55569.5
63	42700.0
64	31370.5
65	23246.5
66	16870.5
67	12305.5
68	9318.0
69	7098.5
70	5326.0
71	4040.0
72	3141.0
73	2426.0
74	1864.5
75	1355.5
76	911.5
77	576.0
78	405.5
79	301.0
80	196.5
81	139.5
82	92.5
83	68.5
84	56.5
85	34.5
86	21.0
87	14.0
88	17.0
89	10.0
90	3.0
91	5.5
92	5.5
93	3.0
94	2.0
95	2.5
96	2.0
97	3.5
98	4.5
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.003673165095317878
2	0.07113545242208204
3	6.802157583921997E-4
4	8.011430043285907E-4
5	0.0018743723120140613
6	6.650998526501508E-4
7	0.0
8	0.001027881590459324
9	1.5115905742048881E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	8.162589100706397E-4
18-19	0.0011185770249116172
20-21	0.003401078791960998
22-23	7.5579528710244404E-6
24-25	1.5115905742048881E-5
26-27	1.1336929306536662E-4
28-29	7.935850514575663E-4
30-31	0.001458684904107717
32-33	0.00311387658286207
34-35	0.004330706995097005
36-37	0.0013604315167843994
38-39	0.0015644962443020593
40-41	0.005517305595847842
42-43	0.007323656332022684
44-45	0.0057591600877206234
46-47	0.007663764211218784
48-49	0.009228260455520844
50-51	0.008548044697128642
52-53	0.004255127466386761
54-55	0.0023958710601147478
56-57	0.0019801836522084035
58-59	0.0030760868185069476
60-61	0.007867828938736443
62-63	0.015047884166209663
64-65	0.004436518335291347
66-67	0.010815430558435975
68-69	0.013468272016165555
70-71	0.006167289542755944
72-73	0.008215494770803567
74-75	0.002962717525441581
76-77	0.0030080652426677274
78-79	0.008147473194964347
80-81	0.005471957878621696
82-83	0.017126321205741386
84-85	0.01854721634549398
86-87	0.02360348681620933
88-89	0.023187799408302987
90-91	0.026218538509583788
92-93	0.0368299043405021
94-95	0.04087340912650018
96-97	0.024714505888249923
98-99	0.019333243444080522
100	0.0196355615589215
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6615548.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.03467036141923
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.11172478297048	47.346428119721885
2	15.8030411093869	19.922789740763207
3	4.793174412728317	9.06408507273356
4	1.776327886453511	4.478809711055744
5	0.8354956576479601	2.6332596684118164
6	0.4595469301718281	1.7380433555389996
7	0.28059816053657666	1.238118878741059
8	0.18419224445171886	0.928839792971524
9	0.13261253487330799	0.7523268679378063
>10	0.5709287291323003	6.22996527828679
>50	0.033159485447251495	1.4388768630862627
>100	0.017484194480816704	2.0414270755572748
>500	0.0011743212948124248	0.4853500638637476
>1k	4.443356435892852E-4	0.52860137846021
>5k	0.0	0.0
>10k+	9.521478076522344E-5	1.1730781328700908
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23331	0.3526691968677425	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20875	0.3155445323652704	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14430	0.21812251985776537	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	3.0231811484097765E-5	0.0	0.0	0.0
6	0.0	3.0231811484097765E-5	0.0	0.0	0.0
7	0.0	3.0231811484097765E-5	0.0	0.0	0.0
8	0.0	3.0231811484097765E-5	0.0	0.0	0.0
9	0.0	3.0231811484097765E-5	0.0	0.0	0.0
10-11	1.5115905742048883E-5	4.534771722614665E-5	0.0	0.0	0.0
12-13	3.0231811484097765E-5	4.534771722614665E-5	0.0	0.0	0.0
14-15	3.0231811484097765E-5	6.046362296819553E-5	0.0	1.5115905742048883E-5	7.557952871024441E-6
16-17	9.06954344522933E-5	7.557952871024441E-5	0.0	6.802157583921998E-5	1.5115905742048883E-5
18-19	9.06954344522933E-5	7.557952871024441E-5	0.0	9.06954344522933E-5	1.5115905742048883E-5
20-21	9.06954344522933E-5	7.557952871024441E-5	0.0	1.2092724593639105E-4	1.5115905742048883E-5
22-23	9.06954344522933E-5	7.557952871024441E-5	0.0	2.0406472751765992E-4	3.7789764355122206E-5
24-25	9.06954344522933E-5	7.557952871024441E-5	0.0	3.174340205830265E-4	6.046362296819553E-5
26-27	1.2092724593639106E-4	7.557952871024441E-5	0.0	5.290567009717109E-4	6.046362296819553E-5
28-29	1.2092724593639106E-4	7.557952871024441E-5	0.0	0.0014511269512366928	6.046362296819553E-5
30-31	1.2092724593639106E-4	7.557952871024441E-5	0.0	0.00513940795229662	6.046362296819553E-5
32-33	1.2092724593639106E-4	7.557952871024441E-5	0.0	0.014292088879107219	6.046362296819553E-5
34-35	1.2092724593639106E-4	7.557952871024441E-5	0.0	0.027609201837852284	6.046362296819553E-5
36-37	1.2092724593639106E-4	9.06954344522933E-5	0.0	0.049549939022436235	7.557952871024441E-5
38-39	1.2092724593639106E-4	1.0581134019434217E-4	0.0	0.08439210175785891	7.557952871024441E-5
40-41	1.2092724593639106E-4	1.1336929306536662E-4	0.0	0.12497075072238914	7.557952871024441E-5
42-43	1.436011045494644E-4	1.2092724593639106E-4	0.0	0.16375060690361554	7.557952871024441E-5
44-45	1.662749631625377E-4	1.2092724593639106E-4	0.0	0.2125069608745942	7.557952871024441E-5
46-47	1.662749631625377E-4	1.2092724593639106E-4	0.0	0.2735752200724717	9.825338732331773E-5
48-49	1.662749631625377E-4	1.2092724593639106E-4	0.0	0.33986602470422705	1.5115905742048882E-4
50-51	1.813908689045866E-4	1.3604315167843993E-4	0.0	0.41222586549141504	1.662749631625377E-4
52-53	1.813908689045866E-4	1.3604315167843993E-4	0.0	0.4864827524492302	2.0406472751765992E-4
54-55	1.813908689045866E-4	1.3604315167843993E-4	0.0	0.5700888271085025	2.2673858613073323E-4
56-57	1.813908689045866E-4	1.3604315167843993E-4	0.0	0.6686747643581454	2.2673858613073323E-4
58-59	1.813908689045866E-4	1.436011045494644E-4	0.0	0.7829358958622928	2.3429653900175766E-4
60-61	2.1162268038868435E-4	1.813908689045866E-4	0.0	0.9052613630798234	2.4185449187278212E-4
62-63	2.4185449187278212E-4	1.813908689045866E-4	0.0	1.0323861303704547	3.174340205830265E-4
64-65	2.494124447438066E-4	1.8894882177561103E-4	0.0	1.1603498304297695	3.174340205830265E-4
66-67	2.6452835048585544E-4	1.9650677464663548E-4	0.0	1.3131338477175285	3.3254992632507537E-4
68-69	2.7208630335687987E-4	1.9650677464663548E-4	0.0	1.4862109684639881	3.476658320671243E-4
70-71	2.7208630335687987E-4	2.0406472751765992E-4	0.0	1.6764446422276733	3.5522378493814876E-4
72-73	3.325499263250754E-4	2.1162268038868435E-4	0.0	1.8700567209247065	4.0812945503531983E-4
74-75	3.325499263250754E-4	2.1162268038868435E-4	0.0	2.0695942346726226	4.0812945503531983E-4
76-77	3.325499263250754E-4	2.1162268038868435E-4	0.0	2.294420658726987	4.0812945503531983E-4
78-79	3.325499263250754E-4	2.1162268038868435E-4	0.0	2.5500684145893886	4.0812945503531983E-4
80-81	3.627817378091732E-4	2.191806332597088E-4	0.0	2.8241349015984767	4.2324536077736875E-4
82-83	3.627817378091732E-4	2.2673858613073323E-4	0.0	3.1079435898583156	4.5347717226146647E-4
84-85	3.627817378091732E-4	2.2673858613073323E-4	0.0	3.3927196960856456	4.5347717226146647E-4
86-87	3.627817378091732E-4	2.2673858613073323E-4	0.0	3.6920750934011815	4.610351251324909E-4
88	3.627817378091732E-4	2.2673858613073323E-4	0.0	3.936756259647727	4.685930780035154E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11755	0.0	48.402344	1
GTATCAA	19945	0.0	35.906208	1
TACCTGG	5800	0.0	31.293427	2
GTACCTG	6375	0.0	29.577932	1
TCAACGC	24215	0.0	29.128237	4
ATCAACG	24225	0.0	29.019224	3
CAACGCA	24515	0.0	28.733446	5
TATCAAC	25480	0.0	27.92115	2
AACGCAG	25340	0.0	27.890688	6
GTACATG	22155	0.0	26.381718	1
TACATGG	22610	0.0	25.455091	2
ACGCAGA	28615	0.0	24.632526	7
ACATGGG	22505	0.0	24.576239	3
ACCTGGG	7295	0.0	24.413603	3
CGCAGAG	28930	0.0	24.26686	8
CATGGGG	12455	0.0	23.354195	4
ATGGGGG	7885	0.0	21.812094	5
GCAGAGT	32635	0.0	21.165989	9
GAGTACT	20000	0.0	20.89988	12-13
AGAGTAC	29985	0.0	19.377668	10-11
>>END_MODULE