FastQCFastQC Report
Mon 6 Jun 2016
ERR1211531_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211531_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6966893
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238890.3428931662937841No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210300.3018562219916396No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137600.19750554515477703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116750.048.8438451
GTATCAA194400.036.5523721
TACCTGG63300.032.7612952
GTACCTG69400.031.2119521
ATCAACG234650.029.7202573
TCAACGC234000.029.7009054
CAACGCA235750.029.4376095
GTACATG228700.029.0936761
AACGCAG244050.028.4497816
TATCAAC248600.028.1278152
TACATGG233500.028.013442
ACCTGGG74100.027.6507853
ACATGGG233250.026.896693
CATGGGG129950.026.397574
ACGCAGA275450.025.0037827
CGCAGAG279950.024.4343998
ATGGGGG85350.022.5161195
GCAGAGT307950.021.3449759
GAGTACT194200.020.83266312-13
CAGAGTA301550.019.59333410-11
Produced by FastQC (version 0.11.5)