FastQCFastQC Report
Mon 6 Jun 2016
ERR1211531_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211531_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6966893
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258460.37098316279581156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232760.33409440908594407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162640.23344696122073355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125800.048.4883421
GTATCAA207750.036.5829161
TACCTGG59550.031.746842
GTACCTG67500.029.2633151
ATCAACG259950.028.7260113
TCAACGC261300.028.6675224
CAACGCA266000.028.1959195
TATCAAC273250.027.6058032
AACGCAG275700.027.2716886
ACCTGGG69800.026.5266283
GTACATG239150.026.0176141
TACATGG243200.024.964272
ACATGGG237100.024.43843
ACGCAGA314300.023.862237
CGCAGAG318400.023.436898
CATGGGG132550.023.2221724
ATGGGGG81000.023.0904875
GCAGAGT353050.020.7372269
GAGTACT218650.020.4821412-13
AGAGTAC328500.019.53344710-11
Produced by FastQC (version 0.11.5)