##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211531_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6966893 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12933972145115 33.0 31.0 34.0 30.0 34.0 2 32.34264283949818 34.0 31.0 34.0 30.0 34.0 3 32.355403190489646 34.0 31.0 34.0 30.0 34.0 4 35.987838194156275 37.0 35.0 37.0 35.0 37.0 5 35.91491271647203 37.0 35.0 37.0 35.0 37.0 6 35.934784989521155 37.0 35.0 37.0 35.0 37.0 7 35.86359988591758 37.0 35.0 37.0 35.0 37.0 8 35.857308702745975 37.0 35.0 37.0 35.0 37.0 9 37.51181064500345 39.0 37.0 39.0 35.0 39.0 10-11 37.47873593006237 39.0 37.0 39.0 34.5 39.0 12-13 37.3946583505732 39.0 37.0 39.0 34.0 39.0 14-15 38.63887553605316 40.0 38.0 41.0 34.0 41.0 16-17 38.5709721679377 40.0 38.0 41.0 34.0 41.0 18-19 38.566225719269696 40.0 38.0 41.0 34.0 41.0 20-21 38.51314179792915 40.0 38.0 41.0 34.0 41.0 22-23 38.40867234504678 40.0 38.0 41.0 34.0 41.0 24-25 38.30878133193663 40.0 38.0 41.0 34.0 41.0 26-27 38.14104931710592 40.0 38.0 41.0 33.0 41.0 28-29 38.17175776920932 40.0 38.0 41.0 33.5 41.0 30-31 38.094974761346265 40.0 37.5 41.0 33.0 41.0 32-33 37.91433577349329 40.0 37.0 41.0 33.0 41.0 34-35 37.80423569014194 40.0 37.0 41.0 33.0 41.0 36-37 37.75598276017731 40.0 37.0 41.0 33.0 41.0 38-39 37.66796619382557 40.0 37.0 41.0 33.0 41.0 40-41 37.51246631748184 40.0 37.0 41.0 32.0 41.0 42-43 37.374163346559214 40.0 36.5 41.0 31.5 41.0 44-45 37.20478712677229 40.0 36.0 41.0 31.0 41.0 46-47 36.96093093434907 39.5 36.0 41.0 30.5 41.0 48-49 36.75785748396021 39.0 36.0 41.0 30.0 41.0 50-51 36.57228092924636 39.0 35.0 41.0 30.0 41.0 52-53 36.393640034374 39.0 35.0 40.5 29.0 41.0 54-55 36.21213581147292 39.0 35.0 40.0 28.5 41.0 56-57 36.03410731297294 39.0 35.0 40.0 28.0 41.0 58-59 35.79842470668058 38.0 34.5 40.0 28.0 41.0 60-61 35.503651125401234 38.0 34.0 40.0 27.0 41.0 62-63 35.1971263947932 38.0 34.0 40.0 26.5 41.0 64-65 34.899218561272576 37.0 34.0 40.0 26.0 41.0 66-67 34.57674683678937 37.0 33.0 39.5 26.0 41.0 68-69 34.18895467463043 36.0 33.0 39.0 26.0 40.5 70-71 33.812370449782996 36.0 32.0 39.0 25.0 40.0 72-73 33.40685367207448 35.0 32.0 38.5 24.5 40.0 74-75 32.99869863079568 35.0 31.5 37.5 24.0 39.5 76-77 31.416686103834234 33.5 29.5 36.0 21.5 38.5 78-79 32.25114365901701 34.5 31.0 36.0 23.5 39.0 80-81 32.2090782792272 35.0 31.0 36.0 23.5 38.5 82-83 31.905383002150316 34.5 31.0 36.0 23.0 37.0 84-85 31.58701934994552 34.0 31.0 35.0 22.0 37.0 86-87 31.278772546097663 34.0 31.0 35.0 20.5 36.5 88-89 30.990015922449217 34.0 30.0 35.0 20.0 36.0 90-91 30.692513506379385 34.0 30.0 35.0 20.0 36.0 92-93 30.47433052294617 34.0 29.5 35.0 19.0 35.0 94-95 30.246505508266022 34.0 29.0 35.0 19.0 35.0 96-97 30.036604552416694 34.0 29.0 35.0 18.0 35.0 98-99 29.873004293305495 34.0 29.0 35.0 18.0 35.0 100 28.022956000616055 32.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32082637917339696 1101 2 0.23379031800539707 1101 3 0.28587993851511584 1101 4 0.140607217644785 1101 5 0.13364820089514495 1101 6 0.14559749547156997 1101 7 0.1863942703814203 1101 8 0.15092252579722754 1101 9 0.15827251054087554 1101 10-11 0.17092980889347587 1101 12-13 0.20894152281941558 1101 14-15 0.29179869744073983 1101 16-17 0.26678472326915426 1101 18-19 0.30310136415052824 1101 20-21 0.258793524608393 1101 22-23 0.25907610344602716 1101 24-25 0.20755384445815395 1101 26-27 0.188545691775154 1101 28-29 0.27815818089464983 1101 30-31 0.2979549570850466 1101 32-33 0.37570669367860177 1101 34-35 0.3393263600584646 1101 36-37 0.39848314454809497 1101 38-39 0.3727065023536298 1101 40-41 0.3274790522222233 1101 42-43 0.398266518371571 1101 44-45 0.4206567150653342 1101 46-47 0.43270746720236986 1101 48-49 0.42957932853256153 1101 50-51 0.3786527430397726 1101 52-53 0.3130379170278914 1101 54-55 0.30699978706110187 1101 56-57 0.33153357879695733 1101 58-59 0.3045688419606165 1101 60-61 0.33476733872979736 1101 62-63 0.36632985007454977 1101 64-65 0.34337601828934083 1101 66-67 0.288982265209782 1101 68-69 0.2829924293557653 1101 70-71 0.319899394472003 1101 72-73 0.11149325877038763 1101 74-75 0.16862343515190759 1101 76-77 0.29962878963065265 1101 78-79 0.3864158273844964 1101 80-81 0.3269931082489421 1101 82-83 0.2559035712047937 1101 84-85 0.2999558991136375 1101 86-87 0.25419914858188264 1101 88-89 0.1661334370863301 1101 90-91 0.1578302092982682 1101 92-93 0.10438597266879412 1101 94-95 0.18337334303856778 1101 96-97 0.09238767398970182 1101 98-99 0.03462343133143264 1101 100 -0.0243710640120689 1102 1 0.2949617314625428 1102 2 0.24595767856754946 1102 3 0.33178698870779044 1102 4 0.2077278127323794 1102 5 0.23980978015972454 1102 6 0.18826766276932716 1102 7 0.16625845605307177 1102 8 0.14238671072079256 1102 9 0.23074912829576277 1102 10-11 0.18948488118135032 1102 12-13 0.22916730387878204 1102 14-15 0.32958450299389597 1102 16-17 0.3176863542951196 1102 18-19 0.2694432745418993 1102 20-21 0.32325978122756993 1102 22-23 0.26307502128602067 1102 24-25 0.24393081923528115 1102 26-27 0.171481197932998 1102 28-29 0.3229654517814282 1102 30-31 0.3516986607673189 1102 32-33 0.44841113200123317 1102 34-35 0.41170033797882155 1102 36-37 0.4193952418350122 1102 38-39 0.44053684045431396 1102 40-41 0.44013969426773514 1102 42-43 0.36909335397095333 1102 44-45 0.4349442243265429 1102 46-47 0.4248698540020186 1102 48-49 0.4882398026532968 1102 50-51 0.5032800676683848 1102 52-53 0.468406204306433 1102 54-55 0.5541505288781394 1102 56-57 0.45913900892897885 1102 58-59 0.4909417662662108 1102 60-61 0.4642526945772474 1102 62-63 0.467070761539901 1102 64-65 0.4636248471715376 1102 66-67 0.4750261317672724 1102 68-69 0.47202970757276574 1102 70-71 0.4677496522306299 1102 72-73 0.43339953765091366 1102 74-75 0.35002641120018296 1102 76-77 0.40669298916150254 1102 78-79 0.4405336706122469 1102 80-81 0.43950588101473187 1102 82-83 0.42587230727361103 1102 84-85 0.35755739393941965 1102 86-87 0.35150606732863565 1102 88-89 0.3364258663662305 1102 90-91 0.30458189354142107 1102 92-93 0.24035183688931383 1102 94-95 0.2961542196157261 1102 96-97 0.3089898874665842 1102 98-99 0.2844064628775662 1102 100 0.30905769902117086 1103 1 0.32685537331062875 1103 2 0.2766064356205362 1103 3 0.3452167156845718 1103 4 0.18101146192485373 1103 5 0.21102389695066393 1103 6 0.23526931496170533 1103 7 0.21207761999793462 1103 8 0.24765010038832713 1103 9 0.29425302008382204 1103 10-11 0.2637138852521659 1103 12-13 0.3124576888853454 1103 14-15 0.3977786484031469 1103 16-17 0.39055639168983447 1103 18-19 0.3524658212504477 1103 20-21 0.32154416588478085 1103 22-23 0.3242439511560917 1103 24-25 0.31716582428453677 1103 26-27 0.3108601607407948 1103 28-29 0.3905220067854174 1103 30-31 0.45328112595012016 1103 32-33 0.4568693656246765 1103 34-35 0.41098626255104165 1103 36-37 0.47200721114835886 1103 38-39 0.4280822312938568 1103 40-41 0.49790550418128987 1103 42-43 0.44372786110271534 1103 44-45 0.445917921210075 1103 46-47 0.5574485698790852 1103 48-49 0.5042581668727522 1103 50-51 0.4760152927610761 1103 52-53 0.4209486301924841 1103 54-55 0.4941855014063634 1103 56-57 0.5440009045427203 1103 58-59 0.5318698040745744 1103 60-61 0.5003369623978031 1103 62-63 0.5525558528127732 1103 64-65 0.531468096845984 1103 66-67 0.6177472334535636 1103 68-69 0.6014599390195841 1103 70-71 0.5313881212368798 1103 72-73 0.4879824034861002 1103 74-75 0.5059482317860855 1103 76-77 0.556561218877416 1103 78-79 0.5827108926010709 1103 80-81 0.5155926516503726 1103 82-83 0.5877735597125415 1103 84-85 0.4527010287556834 1103 86-87 0.41709862105145845 1103 88-89 0.5229683001656689 1103 90-91 0.5273269068959188 1103 92-93 0.42815056201642676 1103 94-95 0.4889256631809644 1103 96-97 0.4699910999197421 1103 98-99 0.46517721602475604 1103 100 0.4940571054344858 1104 1 0.23730809174721657 1104 2 0.20231809736823436 1104 3 0.2422787725350375 1104 4 0.10482864630797195 1104 5 0.15061552937331868 1104 6 0.15836303741618707 1104 7 0.16388080874088473 1104 8 0.17324458522134023 1104 9 0.26055433964896935 1104 10-11 0.2152575713467968 1104 12-13 0.26323357810377956 1104 14-15 0.36630865747632413 1104 16-17 0.3205872688787963 1104 18-19 0.2980869646690181 1104 20-21 0.24146333357762728 1104 22-23 0.29365798769428153 1104 24-25 0.31492067875611696 1104 26-27 0.2597444283213548 1104 28-29 0.40010843006109553 1104 30-31 0.37081621407977394 1104 32-33 0.3469926765733007 1104 34-35 0.4550156306098856 1104 36-37 0.38192844618633615 1104 38-39 0.41960562213247954 1104 40-41 0.5154247258599369 1104 42-43 0.5421786034099227 1104 44-45 0.48446224224748846 1104 46-47 0.4356877700022679 1104 48-49 0.5048403331392564 1104 50-51 0.386677768929367 1104 52-53 0.4384470243277576 1104 54-55 0.4650615946409289 1104 56-57 0.5078544308535058 1104 58-59 0.5893187259690791 1104 60-61 0.5433218128263135 1104 62-63 0.524952968056489 1104 64-65 0.5418423657066782 1104 66-67 0.49375002271970203 1104 68-69 0.5546282739917388 1104 70-71 0.44087921348836545 1104 72-73 0.6050584297810957 1104 74-75 0.5962298538381745 1104 76-77 0.47342090670746373 1104 78-79 0.5556792704275537 1104 80-81 0.6646345101218003 1104 82-83 0.557143747027947 1104 84-85 0.5731311772368883 1104 86-87 0.5928034868395891 1104 88-89 0.6960453362992176 1104 90-91 0.4874944856280976 1104 92-93 0.6400362288360668 1104 94-95 0.6148070840626012 1104 96-97 0.5317959513488439 1104 98-99 0.4786832456194965 1104 100 0.6471245252224413 1105 1 0.21379917878412869 1105 2 0.1657706457299426 1105 3 0.2450772619364372 1105 4 0.16061496326233993 1105 5 0.11422505566137175 1105 6 0.16207755580556693 1105 7 0.1619689440346761 1105 8 0.1896597943687226 1105 9 0.16424947566561343 1105 10-11 0.2030760119139856 1105 12-13 0.2514118293727847 1105 14-15 0.3266242570065998 1105 16-17 0.2825986193147827 1105 18-19 0.25720145826714 1105 20-21 0.22710744124329807 1105 22-23 0.29924459661760494 1105 24-25 0.31001748278681873 1105 26-27 0.29689283342354855 1105 28-29 0.3549990409241701 1105 30-31 0.4048168526025151 1105 32-33 0.4444525935439785 1105 34-35 0.479186415772368 1105 36-37 0.46779599202585587 1105 38-39 0.47446387793804234 1105 40-41 0.4718952274802888 1105 42-43 0.43316110263631913 1105 44-45 0.41621883196138043 1105 46-47 0.47330266464945936 1105 48-49 0.6220327957596297 1105 50-51 0.6433819218490555 1105 52-53 0.5948924780382328 1105 54-55 0.5129286137870324 1105 56-57 0.5794214736401742 1105 58-59 0.6084468385607522 1105 60-61 0.6568581350000713 1105 62-63 0.646405582151516 1105 64-65 0.6293902540512519 1105 66-67 0.6700097753157408 1105 68-69 0.6701904606430915 1105 70-71 0.7243666291056172 1105 72-73 0.6548109152276069 1105 74-75 0.5875897718906344 1105 76-77 0.6086414306351138 1105 78-79 0.7406226515746397 1105 80-81 0.6573231077399484 1105 82-83 0.6334368451600163 1105 84-85 0.682655157049183 1105 86-87 0.6634225900041812 1105 88-89 0.6980465593937772 1105 90-91 0.7604762314706868 1105 92-93 0.6850517292220246 1105 94-95 0.8052089234380624 1105 96-97 0.796679906540767 1105 98-99 0.7570432037045123 1105 100 0.8003754617672563 1106 1 0.2821615147414249 1106 2 0.22155383541471707 1106 3 0.20200542549694944 1106 4 0.13953304661981747 1106 5 0.15431278692339134 1106 6 0.13748014970153832 1106 7 0.15899796628067975 1106 8 0.15401552322469314 1106 9 0.13425020389215803 1106 10-11 0.12886288695017356 1106 12-13 0.20304511576521378 1106 14-15 0.23450320184916507 1106 16-17 0.2753220712080946 1106 18-19 0.27715479649493346 1106 20-21 0.31432887558980127 1106 22-23 0.30276157155072525 1106 24-25 0.268736606914068 1106 26-27 0.27818604769253596 1106 28-29 0.4068861495041318 1106 30-31 0.3874337429812087 1106 32-33 0.38353979404353566 1106 34-35 0.3479475386728268 1106 36-37 0.3521533403913324 1106 38-39 0.36013294954869224 1106 40-41 0.3927428057953932 1106 42-43 0.3833068619465294 1106 44-45 0.42174286903377833 1106 46-47 0.4918485358053104 1106 48-49 0.5352338399010748 1106 50-51 0.46385975937273116 1106 52-53 0.5037428176723182 1106 54-55 0.4930572528642756 1106 56-57 0.5368444892470237 1106 58-59 0.6116183377083644 1106 60-61 0.7094304432667968 1106 62-63 0.7081184981129311 1106 64-65 0.6559243869414004 1106 66-67 0.6074008781561204 1106 68-69 0.6277043663845987 1106 70-71 0.66034520390685 1106 72-73 0.5934519387852504 1106 74-75 0.5918823184574009 1106 76-77 0.5848114606167698 1106 78-79 0.6855004103890465 1106 80-81 0.6854601163096703 1106 82-83 0.6700281562704156 1106 84-85 0.621365255036423 1106 86-87 0.5893937326109757 1106 88-89 0.6180076393468319 1106 90-91 0.6530950992539069 1106 92-93 0.6817256373900307 1106 94-95 0.8262367412775156 1106 96-97 0.8381002267421174 1106 98-99 0.8583373569297343 1106 100 0.9233863980164791 1107 1 0.21915780250920847 1107 2 0.189212017053066 1107 3 0.25515409801259636 1107 4 0.12429516155241771 1107 5 0.10294889515574113 1107 6 0.15279185315997523 1107 7 0.17143664968337902 1107 8 0.17041996648141833 1107 9 0.22808719074441797 1107 10-11 0.19741541952105024 1107 12-13 0.25148958586763115 1107 14-15 0.2794515667381887 1107 16-17 0.21653061121482153 1107 18-19 0.18609771610075398 1107 20-21 0.23389939325898723 1107 22-23 0.31916735978163757 1107 24-25 0.3480582960816392 1107 26-27 0.3805735268932864 1107 28-29 0.3712099849401227 1107 30-31 0.2756685864412134 1107 32-33 0.2861507562246217 1107 34-35 0.34405518337161567 1107 36-37 0.33820308705509916 1107 38-39 0.36852649375535407 1107 40-41 0.4049444374973845 1107 42-43 0.3342069755761514 1107 44-45 0.3695079771546972 1107 46-47 0.35292620361593663 1107 48-49 0.41713770880209466 1107 50-51 0.4181029356720458 1107 52-53 0.41703000390664613 1107 54-55 0.46387449435739825 1107 56-57 0.4567744512680534 1107 58-59 0.5327282300659419 1107 60-61 0.5953012100769328 1107 62-63 0.5131896301766687 1107 64-65 0.6241360254582773 1107 66-67 0.6351846048581891 1107 68-69 0.5575545476405637 1107 70-71 0.6195561458305576 1107 72-73 0.5695696976899427 1107 74-75 0.6585458606455745 1107 76-77 0.5230694376328167 1107 78-79 0.5817197707131143 1107 80-81 0.5405852904708937 1107 82-83 0.5407794992012747 1107 84-85 0.5254581718540052 1107 86-87 0.4908788996665976 1107 88-89 0.40108008907143855 1107 90-91 0.44595497787722493 1107 92-93 0.44277713510673067 1107 94-95 0.5611486139130086 1107 96-97 0.6393932487159937 1107 98-99 0.7586423349999301 1107 100 0.8482702103520232 1108 1 0.22902506252510335 1108 2 0.17203647349523976 1108 3 0.2216587040088811 1108 4 0.14443631821259828 1108 5 0.10684963345006082 1108 6 0.1361131420698456 1108 7 0.10289279026555676 1108 8 0.10731256085023233 1108 9 0.14269255459175412 1108 10-11 0.13859392996234732 1108 12-13 0.17168405317862323 1108 14-15 0.18705112241295296 1108 16-17 0.20025688871339042 1108 18-19 0.24861766424793785 1108 20-21 0.2495482683757615 1108 22-23 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72-73 -0.3646764409658658 2316 74-75 -0.36004870593030347 2316 76-77 -0.23662534237299937 2316 78-79 -0.1634546207649734 2316 80-81 -0.18286845301242494 2316 82-83 -0.04396430240572258 2316 84-85 -0.15070358742661938 2316 86-87 -0.22292672966428384 2316 88-89 -0.15309092368454813 2316 90-91 -0.1009161562671359 2316 92-93 0.04009605730597343 2316 94-95 0.02443528465916245 2316 96-97 0.013432107694644202 2316 98-99 -0.12346298007337708 2316 100 -0.23481329939701467 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 2.0 10 10.0 11 20.0 12 49.0 13 192.0 14 567.0 15 1725.0 16 3888.0 17 8170.0 18 14675.0 19 22419.0 20 31316.0 21 42994.0 22 55022.0 23 62961.0 24 66480.0 25 72549.0 26 84435.0 27 101541.0 28 123996.0 29 149814.0 30 179815.0 31 217814.0 32 267225.0 33 335023.0 34 439786.0 35 597236.0 36 871485.0 37 1399581.0 38 1544894.0 39 271174.0 40 33.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.73613567255223 20.500251483638635 13.940174453855622 23.823438389953516 2 14.652458925031306 21.71439750440461 40.78456472297 22.848578847594077 3 16.919121926783216 27.44434532417472 31.31571033947217 24.3208224095699 4 11.550603658928686 18.071012888468356 39.159789333713036 31.218594118889918 5 11.827567274671566 38.806434828166545 36.08356807848681 13.282429818675068 6 29.524111908283345 38.93112064451528 18.56133505424548 12.983432392955896 7 25.907933421684533 33.466065863219086 23.52080044863614 17.105200266460244 8 24.82771212379579 37.15174251467096 20.946462292452175 17.07408306908108 9 26.29495710496978 15.430828471408553 20.983750140485906 37.29046428313577 10-11 23.78296896478818 26.960310715264324 29.95132119870364 19.30539912124386 12-13 24.468970601385724 24.874840190598594 29.649156374297696 21.007032833717986 14-15 21.82951568224171 26.436332522976887 27.287221147217274 24.44693064756413 16-17 21.188585946302847 29.23431680027559 28.03095373088268 21.54614352253888 18-19 21.035701341715917 28.246740803737712 30.016169488970945 20.701388365575426 20-21 21.903455755799946 27.567278624693834 29.888211482037754 20.641054137468462 22-23 21.553196062663517 27.57430231627659 29.681777062011506 21.190724559048384 24-25 21.28507231057981 27.53349700268068 29.95682292764119 21.22460775909832 26-27 21.300836090601997 27.685450972841032 29.868692458620515 21.145020477936455 28-29 21.261415492343165 27.872676424116044 29.828525037434435 21.03738304610636 30-31 21.71548659476462 27.595250784686726 29.89536218458048 20.793900435968173 32-33 21.38786610809594 27.754896339512936 29.80794591765309 21.049291634738037 34-35 21.53117358864831 27.91192359613043 29.79354881401795 20.76335400120331 36-37 21.537146620298163 27.83438675845058 29.656744748448283 20.971721872802974 38-39 21.473855141761476 28.021863152049715 29.567546203664367 20.93673550252444 40-41 21.638057946832596 27.80628474114207 29.689799389193233 20.865857922832102 42-43 21.391321311186907 27.928422643420486 29.474941293384365 21.205314752008242 44-45 21.330461885528077 28.085039009072034 29.41649278143946 21.168006323960434 46-47 21.331885625845082 27.952484609555 29.219656906818592 21.49597285778133 48-49 21.333396877365853 28.017183588758332 29.261495847579305 21.38792368629651 50-51 21.45652589734655 28.24088531900531 29.01873698421796 21.283851799430188 52-53 21.413530378809096 28.31994980657128 28.90807330755432 21.358446507065302 54-55 21.32930390437771 28.212505009513773 29.080507335995122 21.3776837501134 56-57 21.45767912361867 28.257094172754282 28.744768535178366 21.54045816844868 58-59 21.382647259927655 28.275120460781462 28.955953450754528 21.386278828536348 60-61 21.67661397450094 28.31642401053977 28.869631461072863 21.13733055388643 62-63 21.451076884206415 28.325744014079955 28.963864121517158 21.25931498019647 64-65 21.38117540077821 28.12276141777861 28.92689801090273 21.569165170540447 66-67 21.35793887111054 28.185932539091535 28.786526910542463 21.66960167925546 68-69 21.28559748419096 28.157410186103863 28.951822555445684 21.605169774259497 70-71 21.31104689594171 28.058867073881622 28.931621275448006 21.69846475472866 72-73 21.254380333844168 28.389171165905196 28.57671519891869 21.779733301331945 74-75 21.319778847868736 28.3541735521027 28.528732588145246 21.79731501188332 76-77 21.146945070456802 28.180087158719196 28.73195410376669 21.94101366705731 78-79 21.143635126913185 28.106342246481358 28.731087764457648 22.01893486214781 80-81 21.128321751875237 28.39469356559265 28.467477357967713 22.0095073245644 82-83 21.30806930381078 28.347813378893115 28.509237928833087 21.834879388463023 84-85 21.242478597465826 28.42844131150822 28.357027152526044 21.97205293849991 86-87 21.026238525432213 28.442987677789894 28.415106808351826 22.115666988426067 88-89 21.032944125488303 28.20357106452868 28.54777092010113 22.21571388988189 90-91 21.263139623430337 28.204434278337192 28.482768314211583 22.049657784020884 92-93 21.167501506027662 28.246746883325013 28.41920688358203 22.166544727065286 94-95 20.998095634677757 28.218350471424646 28.375107917026387 22.408445976871207 96-97 20.95532649995011 28.14733432730609 28.45121146710522 22.44612770563858 98-99 20.943848349837744 28.042246083233852 28.368431808398437 22.645473758529967 100 20.16796166667887 28.554617474324317 28.510701892734293 22.766718966262527 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3635.0 1 2588.5 2 2683.0 3 3657.5 4 2704.5 5 2205.5 6 3241.5 7 6175.0 8 9741.0 9 11370.5 10 9691.0 11 7495.5 12 7372.5 13 7799.0 14 8330.0 15 8699.0 16 9182.0 17 9918.5 18 10786.0 19 11779.5 20 13120.5 21 15269.5 22 18555.0 23 23058.0 24 28756.5 25 36351.5 26 46150.5 27 57864.5 28 70905.0 29 84902.5 30 101579.5 31 118382.0 32 134710.0 33 152207.5 34 170375.5 35 186956.5 36 204675.5 37 222323.5 38 234647.0 39 244131.0 40 248537.5 41 250190.5 42 252959.0 43 256910.0 44 260092.5 45 262471.0 46 263863.0 47 263641.5 48 263618.0 49 261543.5 50 255449.5 51 242536.0 52 225102.5 53 207640.0 54 189124.0 55 169383.5 56 149064.5 57 129903.0 58 111786.0 59 92067.0 60 72946.0 61 57893.0 62 45801.0 63 34825.5 64 25543.0 65 18713.0 66 13731.0 67 10192.5 68 7560.0 69 5522.5 70 4204.0 71 3230.5 72 2426.0 73 1802.0 74 1355.0 75 986.5 76 682.0 77 437.5 78 273.5 79 201.0 80 141.5 81 99.5 82 70.0 83 50.0 84 38.5 85 30.0 86 19.5 87 15.5 88 13.0 89 7.5 90 5.0 91 3.5 92 3.0 93 3.0 94 4.0 95 3.0 96 1.5 97 3.0 98 4.0 99 3.5 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0034018033576803893 2 0.06985897443810318 3 6.889728319352687E-4 4 6.459120299393144E-4 5 0.0016937248785075356 6 6.459120299393144E-4 7 0.0 8 9.329840432456763E-4 9 2.8707201330636195E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.172048286086782E-4 18-19 9.760448452416306E-4 20-21 0.0030357865407147776 22-23 7.176800332659049E-6 24-25 1.4353600665318098E-5 26-27 6.459120299393144E-5 28-29 6.459120299393144E-4 30-31 0.0014353600665318097 32-33 0.002633885722085871 34-35 0.004054892187952363 36-37 0.0013635920632052193 38-39 0.001471244068195105 40-41 0.005296478645502378 42-43 0.006774899514030142 44-45 0.0053467162478309915 46-47 0.006839490717024074 48-49 0.008540392395864268 50-51 0.008475801192870336 52-53 0.003502278562337616 54-55 0.0021386864991323963 56-57 0.0022104545024589873 58-59 0.002964018537388187 60-61 0.007449518745300093 62-63 0.013822517440701329 64-65 0.004169720993274907 66-67 0.01019823327270851 68-69 0.012394334174502177 70-71 0.005511782655482149 72-73 0.007593054751953274 74-75 0.002806128930069688 76-77 0.0025477641180939622 78-79 0.007700706756943159 80-81 0.005303655445835037 82-83 0.015788960731849908 84-85 0.01704490079006524 86-87 0.022154782626918484 88-89 0.02099931777336038 90-91 0.024415474731706084 92-93 0.03415439278312442 94-95 0.037757146550119254 96-97 0.022649981849871957 98-99 0.0176477520180086 100 0.018028122435639532 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6966893.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.344445703397135 #Duplication Level Percentage of deduplicated Percentage of total 1 74.75714906117989 47.35450169646676 2 16.230244205671067 20.56191645678014 3 4.862529188669359 9.240426485185498 4 1.7695468796078133 4.483638649397318 5 0.8175234605925594 2.589278523037935 6 0.43093773591735335 1.6378507208617006 7 0.25929052529903324 1.1497230220846952 8 0.18114314184158856 0.9179529530341816 9 0.11438221338181903 0.6520930114499118 >10 0.5274216709583402 5.691453533201259 >50 0.03139645952681074 1.3837485129556137 >100 0.01679283435834696 2.0366732700192647 >500 0.0011146404367030193 0.46912216364499926 >1k 4.106531017128995E-4 0.5220727435570797 >5k 2.9332364402561694E-5 0.09464867803491171 >10k+ 8.799709320768508E-5 1.2148995802887195 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25846 0.37098316279581156 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23276 0.33409440908594407 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16264 0.23344696122073355 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 1.4353600665318098E-5 0.0 0.0 0.0 8 0.0 1.4353600665318098E-5 0.0 0.0 0.0 9 0.0 1.4353600665318098E-5 0.0 1.4353600665318098E-5 0.0 10-11 0.0 1.4353600665318098E-5 0.0 2.8707201330636195E-5 0.0 12-13 0.0 1.4353600665318098E-5 0.0 2.8707201330636195E-5 0.0 14-15 0.0 2.1530400997977146E-5 0.0 2.8707201330636195E-5 1.4353600665318098E-5 16-17 0.0 2.8707201330636195E-5 0.0 2.8707201330636195E-5 1.4353600665318098E-5 18-19 0.0 2.8707201330636195E-5 0.0 2.8707201330636195E-5 1.4353600665318098E-5 20-21 0.0 7.176800332659049E-5 0.0 5.023760232861334E-5 1.4353600665318098E-5 22-23 0.0 8.612160399190858E-5 0.0 1.5071280698584003E-4 1.4353600665318098E-5 24-25 0.0 8.612160399190858E-5 0.0 2.583648119757258E-4 1.4353600665318098E-5 26-27 0.0 1.0047520465722669E-4 0.0 4.019008186289067E-4 1.4353600665318098E-5 28-29 0.0 1.0047520465722669E-4 0.0 0.0010765200498988573 1.4353600665318098E-5 30-31 0.0 1.148288053225448E-4 0.0 0.004614682613899769 1.4353600665318098E-5 32-33 0.0 1.291824059878629E-4 0.0 0.01372921903637676 1.4353600665318098E-5 34-35 0.0 1.5071280698584003E-4 0.0 0.02714983565844918 1.4353600665318098E-5 36-37 0.0 1.9377360898179433E-4 0.0 0.04862282225376506 1.4353600665318098E-5 38-39 0.0 2.0095040931445338E-4 0.0 0.08400444789377418 1.4353600665318098E-5 40-41 0.0 2.3683441097774863E-4 0.0 0.1257088346268559 1.4353600665318098E-5 42-43 0.0 2.7271841264104387E-4 0.0 0.1676285253699174 1.4353600665318098E-5 44-45 0.0 2.7271841264104387E-4 0.0 0.2194019629697198 1.4353600665318098E-5 46-47 0.0 2.7271841264104387E-4 0.0 0.280799489815618 1.4353600665318098E-5 48-49 0.0 2.7271841264104387E-4 0.0 0.34839059534860084 1.4353600665318098E-5 50-51 0.0 2.798952129737029E-4 0.0 0.42321591561690414 1.4353600665318098E-5 52-53 0.0 2.8707201330636196E-4 0.0 0.4998354359683721 2.8707201330636195E-5 54-55 0.0 2.94248813639021E-4 0.0 0.5871986264178307 4.306080199595429E-5 56-57 0.0 3.0142561397168005E-4 0.0 0.688291035903666 4.306080199595429E-5 58-59 0.0 3.0142561397168005E-4 0.0 0.8048781573077124 4.306080199595429E-5 60-61 0.0 3.0142561397168005E-4 0.0 0.9281971174237929 4.306080199595429E-5 62-63 0.0 3.1577921463699814E-4 0.0 1.0594033811054655 8.612160399190858E-5 64-65 0.0 3.4448641596763433E-4 0.0 1.1913703856224003 8.612160399190858E-5 66-67 0.0 3.4448641596763433E-4 0.0 1.3479035776780266 8.612160399190858E-5 68-69 0.0 3.4448641596763433E-4 0.0 1.5286154100543814 8.612160399190858E-5 70-71 0.0 3.4448641596763433E-4 0.0 1.7261640160111544 8.612160399190858E-5 72-73 0.0 3.4448641596763433E-4 0.0 1.9231384779413148 1.0047520465722669E-4 74-75 0.0 3.4448641596763433E-4 0.0 2.122639173588571 1.0047520465722669E-4 76-77 0.0 3.4448641596763433E-4 0.0 2.348729914468329 1.0047520465722669E-4 78-79 0.0 3.4448641596763433E-4 0.0 2.609742965766806 1.0047520465722669E-4 80-81 0.0 3.5166321630029343E-4 0.0 2.888095166668987 1.0765200498988573E-4 82-83 0.0 3.588400166329525E-4 0.0 3.1774278720801368 1.1482880532254478E-4 84-85 0.0 3.588400166329525E-4 0.0 3.4731335761866875 1.1482880532254478E-4 86-87 0.0 3.588400166329525E-4 0.0 3.7840182129968123 1.1482880532254478E-4 88 0.0 3.588400166329525E-4 0.0 4.033490969360374 1.1482880532254478E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12580 0.0 48.488342 1 GTATCAA 20775 0.0 36.582916 1 TACCTGG 5955 0.0 31.74684 2 GTACCTG 6750 0.0 29.263315 1 ATCAACG 25995 0.0 28.726011 3 TCAACGC 26130 0.0 28.667522 4 CAACGCA 26600 0.0 28.195919 5 TATCAAC 27325 0.0 27.605803 2 AACGCAG 27570 0.0 27.271688 6 ACCTGGG 6980 0.0 26.526628 3 GTACATG 23915 0.0 26.017614 1 TACATGG 24320 0.0 24.96427 2 ACATGGG 23710 0.0 24.4384 3 ACGCAGA 31430 0.0 23.86223 7 CGCAGAG 31840 0.0 23.43689 8 CATGGGG 13255 0.0 23.222172 4 ATGGGGG 8100 0.0 23.090487 5 GCAGAGT 35305 0.0 20.737226 9 GAGTACT 21865 0.0 20.48214 12-13 AGAGTAC 32850 0.0 19.533447 10-11 >>END_MODULE