FastQCFastQC Report
Mon 6 Jun 2016
ERR1211530_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211530_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10219603
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT374940.36688313626272956No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT329240.32216515651341837No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217710.21303175867007748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA179950.050.4232941
GTATCAA306200.038.137111
TCAACGC375000.030.3488314
CAACGCA377350.030.132865
ATCAACG378750.030.0741923
GTACATG381350.029.5227761
AACGCAG392300.029.0986146
TATCAAC395850.028.8650592
TACATGG387250.028.44972
ACATGGG382300.027.4963913
TACCTGG88200.027.1360112
CATGGGG218400.025.9904274
GTACCTG96050.025.49221
ACGCAGA443000.025.451117
CGCAGAG450050.024.8231378
ATGGGGG137550.022.1352165
GCAGAGT500650.021.6873979
GAGTACT295650.021.39116312-13
ACCTGGG109550.020.975413
CAGAGTA486500.019.47394410-11
Produced by FastQC (version 0.11.5)