FastQCFastQC Report
Mon 6 Jun 2016
ERR1211530_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211530_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10219603
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT415660.4067281282844353No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT372850.3648380470356823No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261760.25613519429277243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA203150.048.750551
GTATCAA331800.035.7868081
TCAACGC420600.027.8308014
ATCAACG422900.027.6019293
CAACGCA425350.027.4868665
TACCTGG83950.027.279452
TATCAAC438150.026.8314742
AACGCAG440450.026.5976146
GTACATG401150.026.5052431
TACATGG408300.025.5797632
ACATGGG399700.025.1714363
GTACCTG97100.024.118381
CATGGGG222100.023.9507524
TATAACG8850.023.3795832
ACGCAGA503450.023.0917117
CGCAGAG511450.022.6018878
ATGGGGG132450.021.0744275
ACCTGGG109000.020.5644653
GAGTACT335900.020.36194212-13
GCAGAGT568450.020.0294789
Produced by FastQC (version 0.11.5)